Restriction map for
pF1KB8252
(Circular) (Six-base) MAPSORT of: pF1KB8252.seq Check: 3161 from: 1 to: 4118
pF1KB8252 4118 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1156 1156
Size: 4118
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1943 2375 3122 3390 1943
Size: 432 747 268 2671
Fragments arranged by size:
2671 747 432 268
AflIII A'CryG_T
Cuts at: 242 824 1470 2574 3551 242
Size: 582 646 1104 977 809
Fragments arranged by size:
1104 977 809 646 582
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AhdI GACnn_n'nnGTC
Cuts at: 848 848
Size: 4118
AlwNI CAG_nnn'CTG
Cuts at: 1423 2990 1423
Size: 1567 2551
ApaI G_GGCC'C
Cuts at: 913 913
Size: 4118
ApaLI G'TGCA_C
Cuts at: 2888 2888
Size: 4118
ApoI r'AATT_y
Cuts at: 271 1057 1122 3471 3727 271
Size: 786 65 2349 256 662
Fragments arranged by size:
2349 786 662 256 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 1138 1138
Size: 4118
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 597 630 597
Size: 33 4085
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 152 280 1134 1548 1796 1831 152
Size: 128 854 414 248 35 2439
Fragments arranged by size:
2439 854 414 248 128 35
BanII G_rGCy'C
Cuts at: 438 714 913 1132 2162 438
Size: 276 199 219 1030 2394
Fragments arranged by size:
2394 1030 276 219 199
BbeI G_GCGC'C
Cuts at: 156 1552 1800 156
Size: 1396 248 2474
Fragments arranged by size:
2474 1396 248
BbsI GAAGACnn'nnnn_
Cuts at: 402 670 402
Size: 268 3850
BciVI GTATCCnnnnn_n'
Cuts at: 2016 2777 4118 2016
Size: 761 1341 2016
Fragments arranged by size:
2016 1341 761
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglI GCCn_nnn'nGGC
Cuts at: 1099 1099
Size: 4118
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 576 1230 576
Size: 654 3464
Bme1580I G_kGCm'C
Cuts at: 913 1743 1836 2892 913
Size: 830 93 1056 2139
Fragments arranged by size:
2139 1056 830 93
BmrI ACTGGGnnnn_n'
Cuts at: 1330 1411 1746 3890 1330
Size: 81 335 2144 1558
Fragments arranged by size:
2144 1558 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2485 2485
Size: 4118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 4118
Bpu10I CC'TnA_GC
Cuts at: 773 773
Size: 4118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 901 1294 2173 2665 2963 523
Size: 378 393 879 492 298 1678
Fragments arranged by size:
1678 879 492 393 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 957 3822 957
Size: 2865 1253
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 153 407 901 1549 1797 2499 3995 153
Size: 254 494 648 248 702 1496 276
Fragments arranged by size:
1496 702 648 494 276 254 248
BsaWI w'CCGG_w
Cuts at: 1307 1828 2506 2780 2927 3338 1307
Size: 521 678 274 147 411 2087
Fragments arranged by size:
2087 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 964 994 1087 1117 964
Size: 30 93 30 3965
Fragments arranged by size:
3965 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 945 972 945
Size: 27 4091
BseYI C'CCAG_C
Cuts at: 430 997 1543 2301 2878 3374 430
Size: 567 546 758 577 496 1174
Fragments arranged by size:
1174 758 577 567 546 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 546
Size: 4118
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 944 1132 1910 2100 2892 944
Size: 188 778 190 792 2170
Fragments arranged by size:
2170 792 778 190 188
Bsp1286I G_dGCh'C
Cuts at: 438 714 913 944 1132 1743 1836 1910 2100
Size: 276 199 31 188 611 93 74 190
Cuts at: 2100 2162 2892 438
Size: 62 730 1664
Fragments arranged by size:
1664 730 611 276 199 190 188 93
74 62 31
BspEI T'CCGG_A
Cuts at: 3338 3338
Size: 4118
BspHI T'CATG_A
Cuts at: 629 3294 629
Size: 2665 1453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 853 870 1168 1382 1684 2065 853
Size: 17 298 214 302 381 2906
Fragments arranged by size:
2906 381 302 298 214 17
BsrBI CCG'CTC
Cuts at: 50 2410 2464 3948 4113 50
Size: 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 558 1007 2030 558
Size: 449 1023 2646
Fragments arranged by size:
2646 1023 449
BsrFI r'CCGG_y
Cuts at: 2116 2297 2506 2116
Size: 181 209 3728
Fragments arranged by size:
3728 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstEII G'GTnAC_C
Cuts at: 159 159
Size: 4118
BstXI CCAn_nnnn'nTGG
Cuts at: 756 756
Size: 4118
BstYI r'GATC_y
Cuts at: 1 348 1143 1610 1633 1968 2214 3226 3312
Size: 347 795 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 795 467 449 347 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 264 276 419 705 107
Size: 157 12 143 286 3520
Fragments arranged by size:
3520 286 157 143 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 386 2068 2249 2412 386
Size: 1682 181 163 2092
Fragments arranged by size:
2092 1682 181 163
BtsI GCAGTG_nn'
Cuts at: 650 815 1361 650
Size: 165 546 3407
Fragments arranged by size:
3407 546 165
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DraIII CAC_nnn'GTG
Cuts at: 948 948
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1027 1824 2682 1027
Size: 797 858 2463
Fragments arranged by size:
2463 858 797
EaeI y'GGCC_r
Cuts at: 587 702 1703 1877 2268 2295 3640 587
Size: 115 1001 174 391 27 1345 1065
Fragments arranged by size:
1345 1065 1001 391 174 115 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2326 2636 2782 3928 2326
Size: 310 146 1146 2516
Fragments arranged by size:
2516 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1943 2375 3122 3390 700
Size: 1243 432 747 268 1428
Fragments arranged by size:
1428 1243 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 589 589
Size: 4118
EcoO109I rG'GnC_Cy
Cuts at: 909 910 1257 909
Size: 1 347 3770
Fragments arranged by size:
3770 347 1
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 4118
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 156 383 746 1552 1800 2822 3801 156
Size: 227 363 806 248 1022 979 473
Fragments arranged by size:
1022 979 806 473 363 248 227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 664 696 834 866 964 996 1960 1990 1992
Size: 32 138 32 98 32 964 30 2
Cuts at: 1992 2022 664
Size: 30 2760
Fragments arranged by size:
2760 964 138 98 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 132 1157 132
Size: 1025 3093
HindIII A'AGCT_T
Cuts at: 304 304
Size: 4118
KasI G'GCGC_C
Cuts at: 152 1548 1796 152
Size: 1396 248 2474
Fragments arranged by size:
2474 1396 248
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 105 884 2789 2973 105
Size: 779 1905 184 1250
Fragments arranged by size:
1905 1250 779 184
MscI TGG'CCA
Cuts at: 589 1879 589
Size: 1290 2828
MslI CAynn'nnrTG
Cuts at: 418 508 796 2107 2234 418
Size: 90 288 1311 127 2302
Fragments arranged by size:
2302 1311 288 127 90
MspA1I CmG'CkG
Cuts at: 707 1229 1543 1903 2916 3161 707
Size: 522 314 360 1013 245 1664
Fragments arranged by size:
1664 1013 522 360 314 245
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 153 1549 1797 153
Size: 1396 248 2474
Fragments arranged by size:
2474 1396 248
NcoI C'CATG_G
Cuts at: 107 264 276 419 107
Size: 157 12 143 3806
Fragments arranged by size:
3806 157 143 12
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NspI r_CATG'y
Cuts at: 246 1171 1671 2578 246
Size: 925 500 907 1786
Fragments arranged by size:
1786 925 907 500
PciI A'CATG_T
Cuts at: 242 2574 242
Size: 2332 1786
PfoI T'CCnGG_A
Cuts at: 338 682 338
Size: 344 3774
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3257 3289 3257
Size: 32 4086
PshAI GACnn'nnGTC
Cuts at: 637 637
Size: 4118
PspOMI G'GGCC_C
Cuts at: 909 909
Size: 4118
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 1543 1903 1543
Size: 360 3758
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SacII CC_GC'GG
Cuts at: 708 708
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SexAI A'CCwGG_T
Cuts at: 944 944
Size: 4118
SfcI C'TryA_G
Cuts at: 33 1065 1161 1846 2839 3030 33
Size: 1032 96 685 993 191 1121
Fragments arranged by size:
1121 1032 993 685 191 96
SfiI GGCCn_nnn'nGGCC
Cuts at: 1099 1099
Size: 4118
SfoI GGC'GCC
Cuts at: 154 1550 1798 154
Size: 1396 248 2474
Fragments arranged by size:
2474 1396 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 1140 1140
Size: 4118
SmlI C'TyrA_G
Cuts at: 538 916 1273 2188 2680 2942 538
Size: 378 357 915 492 262 1714
Fragments arranged by size:
1714 915 492 378 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StyI C'CwwG_G
Cuts at: 107 264 276 419 590 749 1106 1252 1606
Size: 157 12 143 171 159 357 146 354
Cuts at: 1606 107
Size: 2619
Fragments arranged by size:
2619 357 354 171 159 157 146 143
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 277 496 673 991 1701 1834 2293 2502 277
Size: 219 177 318 710 133 459 209 1893
Fragments arranged by size:
1893 710 459 318 219 209 177 133
TatI w'GTAC_w
Cuts at: 3633 3633
Size: 4118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1087 1119 3635 3667 1087
Size: 32 2516 32 1538
Fragments arranged by size:
2516 1538 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 852 1149 852
Size: 297 3821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 607 828 1613 501
Size: 106 221 785 3006
Fragments arranged by size:
3006 785 221 106
XmaI C'CCGG_G
Cuts at: 1138 1138
Size: 4118
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PsiI PspXI
PsrI SanDI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI