Restriction map for
pF1KB8253
(Circular) (Six-base) MAPSORT of: pF1KB8253.seq Check: 3441 from: 1 to: 3341
pF1KB8253 3341 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 379 379
Size: 3341
Acc65I G'GTAC_C
Cuts at: 357 357
Size: 3341
AclI AA'CG_TT
Cuts at: 3181 3181
Size: 3341
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1166 1598 2345 2613 1166
Size: 432 747 268 1894
Fragments arranged by size:
1894 747 432 268
AflIII A'CryG_T
Cuts at: 693 1797 2774 693
Size: 1104 977 1260
Fragments arranged by size:
1260 1104 977
AgeI A'CCGG_T
Cuts at: 1729 1729
Size: 3341
AlwNI CAG_nnn'CTG
Cuts at: 646 2213 646
Size: 1567 1774
ApaLI G'TGCA_C
Cuts at: 2111 2111
Size: 3341
ApoI r'AATT_y
Cuts at: 210 345 2694 2950 210
Size: 135 2349 256 601
Fragments arranged by size:
2349 601 256 135
AseI AT'TA_AT
Cuts at: 20 20
Size: 3341
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3341
AvaI C'yCGr_G
Cuts at: 361 361
Size: 3341
BamHI G'GATC_C
Cuts at: 366 366
Size: 3341
BanI G'GyrC_C
Cuts at: 357 771 1019 1054 357
Size: 414 248 35 2644
Fragments arranged by size:
2644 414 248 35
BanII G_rGCy'C
Cuts at: 355 1385 355
Size: 1030 2311
BbeI G_GCGC'C
Cuts at: 775 1023 775
Size: 248 3093
BciVI GTATCCnnnnn_n'
Cuts at: 1239 2000 3341 1239
Size: 761 1341 1239
Fragments arranged by size:
1341 1239 761
BclI T'GATC_A
Cuts at: 861 861
Size: 3341
BglII A'GATC_T
Cuts at: 856 2893 856
Size: 2037 1304
BlpI GC'TnA_GC
Cuts at: 453 453
Size: 3341
Bme1580I G_kGCm'C
Cuts at: 966 1059 2115 966
Size: 93 1056 2192
Fragments arranged by size:
2192 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 280 553 634 969 3113 280
Size: 273 81 335 2144 508
Fragments arranged by size:
2144 508 335 273 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1708 1708
Size: 3341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 517 1396 1888 2186 148
Size: 369 879 492 298 1303
Fragments arranged by size:
1303 879 492 369 298
BsaI GGTCTCn'nnnn_
Cuts at: 3045 3045
Size: 3341
BsaAI yAC'GTr
Cuts at: 694 694
Size: 3341
BsaBI GATnn'nnATC
Cuts at: 6 855 2892 6
Size: 849 2037 455
Fragments arranged by size:
2037 849 455
BsaHI Gr'CG_yC
Cuts at: 772 1020 1722 3218 772
Size: 248 702 1496 895
Fragments arranged by size:
1496 895 702 248
BsaWI w'CCGG_w
Cuts at: 530 1051 1729 2003 2150 2561 530
Size: 521 678 274 147 411 1310
Fragments arranged by size:
1310 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 766 1524 2101 2597 766
Size: 758 577 496 1510
Fragments arranged by size:
1510 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 883 929 2137 104
Size: 779 46 1208 1308
Fragments arranged by size:
1308 1208 779 46
BsiHKAI G_wGCw'C
Cuts at: 355 1133 1323 2115 355
Size: 778 190 792 1581
Fragments arranged by size:
1581 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 355 966 1059 1133 1323 1385 2115 355
Size: 611 93 74 190 62 730 1581
Fragments arranged by size:
1581 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2561 2561
Size: 3341
BspHI T'CATG_A
Cuts at: 2517 2517
Size: 3341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 391 605 907 1288 391
Size: 214 302 381 2444
Fragments arranged by size:
2444 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1633 1687 3171 3336 50
Size: 1583 54 1484 165 55
Fragments arranged by size:
1583 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1253 1253
Size: 3341
BsrFI r'CCGG_y
Cuts at: 1339 1520 1729 1339
Size: 181 209 2951
Fragments arranged by size:
2951 209 181
BssSI C'ACGA_G
Cuts at: 1612 1970 1612
Size: 358 2983
BstAPI GCAn_nnn'nTGC
Cuts at: 718 718
Size: 3341
BstBI TT'CG_AA
Cuts at: 1702 1702
Size: 3341
BstYI r'GATC_y
Cuts at: 1 366 833 856 1191 1437 2449 2535 2547
Size: 365 467 23 335 246 1012 86 12
Cuts at: 2547 2893 1
Size: 346 449
Fragments arranged by size:
1012 467 449 365 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1291 1472 1635 1291
Size: 181 163 2997
Fragments arranged by size:
2997 181 163
BtsI GCAGTG_nn'
Cuts at: 132 584 132
Size: 452 2889
DraI TTT'AAA
Cuts at: 340 340
Size: 3341
DrdI GACnn_nn'nnGTC
Cuts at: 1047 1905 1047
Size: 858 2483
EaeI y'GGCC_r
Cuts at: 926 1100 1491 1518 2863 926
Size: 174 391 27 1345 1404
Fragments arranged by size:
1404 1345 391 174 27
EagI C'GGCC_G
Cuts at: 926 926
Size: 3341
EarI CTCTTCn'nnn_
Cuts at: 1364 1574 1364
Size: 210 3131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1549 1859 2005 3151 1549
Size: 310 146 1146 1739
Fragments arranged by size:
1739 1146 310 146
EcoICRI GAG'CTC
Cuts at: 353 353
Size: 3341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1166 1598 2345 2613 1166
Size: 432 747 268 1894
Fragments arranged by size:
1894 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 480 480
Size: 3341
EcoRI G'AATT_C
Cuts at: 210 345 210
Size: 135 3206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1360 1360
Size: 3341
FspI TGC'GCA
Cuts at: 1122 1122
Size: 3341
HaeII r_GCGC'y
Cuts at: 775 1023 2045 3024 775
Size: 248 1022 979 1092
Fragments arranged by size:
1092 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 144 176 1183 1213 1215 1245 144
Size: 32 1007 30 2 30 2240
Fragments arranged by size:
2240 1007 32 30 30 2
HincII GTy'rAC
Cuts at: 380 380
Size: 3341
KasI G'GCGC_C
Cuts at: 771 1019 771
Size: 248 3093
KpnI G_GTAC'C
Cuts at: 361 361
Size: 3341
MluI A'CGCG_T
Cuts at: 2774 2774
Size: 3341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2196 2012
Size: 184 3157
MscI TGG'CCA
Cuts at: 1102 1102
Size: 3341
MslI CAynn'nnrTG
Cuts at: 1330 1457 1330
Size: 127 3214
MspA1I CmG'CkG
Cuts at: 452 766 1126 2139 2384 452
Size: 314 360 1013 245 1409
Fragments arranged by size:
1409 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1522 1522
Size: 3341
NarI GG'CG_CC
Cuts at: 772 1020 772
Size: 248 3093
NgoMIV G'CCGG_C
Cuts at: 1520 1520
Size: 3341
NspI r_CATG'y
Cuts at: 394 894 1801 394
Size: 500 907 1934
Fragments arranged by size:
1934 907 500
PciI A'CATG_T
Cuts at: 1797 1797
Size: 3341
PmeI GTTT'AAAC
Cuts at: 340 340
Size: 3341
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2480 2512 2480
Size: 32 3309
PstI C_TGCA'G
Cuts at: 388 1073 388
Size: 685 2656
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3341
PvuII CAG'CTG
Cuts at: 766 1126 766
Size: 360 2981
RsrII CG'GwC_CG
Cuts at: 1536 1536
Size: 3341
SacI G_AGCT'C
Cuts at: 355 355
Size: 3341
SalI G'TCGA_C
Cuts at: 378 378
Size: 3341
SapI GCTCTTCn'nnn_
Cuts at: 1364 1574 1364
Size: 210 3131
SbfI CC_TGCA'GG
Cuts at: 388 388
Size: 3341
ScaI AGT'ACT
Cuts at: 2858 2858
Size: 3341
SfcI C'TryA_G
Cuts at: 33 384 1069 2062 2253 33
Size: 351 685 993 191 1121
Fragments arranged by size:
1121 993 685 351 191
SfoI GGC'GCC
Cuts at: 773 1021 773
Size: 248 3093
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3341
SmaI CCC'GGG
Cuts at: 363 363
Size: 3341
SmlI C'TyrA_G
Cuts at: 163 496 1411 1903 2165 163
Size: 333 915 492 262 1339
Fragments arranged by size:
1339 915 492 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3341
SphI G_CATG'C
Cuts at: 394 894 394
Size: 500 2841
StyI C'CwwG_G
Cuts at: 475 829 475
Size: 354 2987
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 924 1057 1516 1725 924
Size: 133 459 209 2540
Fragments arranged by size:
2540 459 209 133
TatI w'GTAC_w
Cuts at: 2856 2856
Size: 3341
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3309
Tth111I GACn'n_nGTC
Cuts at: 1138 1138
Size: 3341
XbaI T'CTAG_A
Cuts at: 372 372
Size: 3341
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 836 836
Size: 3341
XmaI C'CCGG_G
Cuts at: 361 361
Size: 3341
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI