Restriction map for
pF1KB8258
(Circular) (Six-base) MAPSORT of: pF1KB8258.seq Check: 6052 from: 1 to: 3830
pF1KB8258 3830 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 528 528
Size: 3830
AccI GT'mk_AC
Cuts at: 868 868
Size: 3830
Acc65I G'GTAC_C
Cuts at: 846 846
Size: 3830
AclI AA'CG_TT
Cuts at: 3670 3670
Size: 3830
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1655 2087 2834 3102 1655
Size: 432 747 268 2383
Fragments arranged by size:
2383 747 432 268
AflIII A'CryG_T
Cuts at: 1182 2286 3263 1182
Size: 1104 977 1749
Fragments arranged by size:
1749 1104 977
AgeI A'CCGG_T
Cuts at: 2218 2218
Size: 3830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 599 692 599
Size: 93 3737
AlwNI CAG_nnn'CTG
Cuts at: 358 376 1135 2702 358
Size: 18 759 1567 1486
Fragments arranged by size:
1567 1486 759 18
ApaLI G'TGCA_C
Cuts at: 2600 2600
Size: 3830
ApoI r'AATT_y
Cuts at: 796 834 3183 3439 796
Size: 38 2349 256 1187
Fragments arranged by size:
2349 1187 256 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 3830
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
AvaI C'yCGr_G
Cuts at: 131 850 131
Size: 719 3111
BamHI G'GATC_C
Cuts at: 855 855
Size: 3830
BanI G'GyrC_C
Cuts at: 846 1260 1508 1543 846
Size: 414 248 35 3133
Fragments arranged by size:
3133 414 248 35
BanII G_rGCy'C
Cuts at: 607 844 1874 607
Size: 237 1030 2563
Fragments arranged by size:
2563 1030 237
BbeI G_GCGC'C
Cuts at: 1264 1512 1264
Size: 248 3582
BbsI GAAGACnn'nnnn_
Cuts at: 491 491
Size: 3830
BciVI GTATCCnnnnn_n'
Cuts at: 1728 2489 3830 1728
Size: 761 1341 1728
Fragments arranged by size:
1728 1341 761
BclI T'GATC_A
Cuts at: 1350 1350
Size: 3830
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3830
BglII A'GATC_T
Cuts at: 1345 3382 1345
Size: 2037 1793
BlpI GC'TnA_GC
Cuts at: 351 942 351
Size: 591 3239
Bme1580I G_kGCm'C
Cuts at: 1455 1548 2604 1455
Size: 93 1056 2681
Fragments arranged by size:
2681 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 192 215 284 1042 1123 1458 3602 192
Size: 23 69 758 81 335 2144 420
Fragments arranged by size:
2144 758 420 335 81 69 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 3830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 246 699 161
Size: 85 453 3292
Fragments arranged by size:
3292 453 85
Bpu10I CC'TnA_GC
Cuts at: 345 345
Size: 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 1006 1885 2377 2675 509
Size: 497 879 492 298 1664
Fragments arranged by size:
1664 879 497 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 776 3534 776
Size: 2758 1072
BsaAI yAC'GTr
Cuts at: 1183 1183
Size: 3830
BsaBI GATnn'nnATC
Cuts at: 6 1344 3381 6
Size: 1338 2037 455
Fragments arranged by size:
2037 1338 455
BsaHI Gr'CG_yC
Cuts at: 1261 1509 2211 3707 1261
Size: 248 702 1496 1384
Fragments arranged by size:
1496 1384 702 248
BsaWI w'CCGG_w
Cuts at: 1019 1540 2218 2492 2639 3050 1019
Size: 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 524 554 524
Size: 30 3800
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 737 737
Size: 3830
BseYI C'CCAG_C
Cuts at: 515 1255 2013 2590 3086 515
Size: 740 758 577 496 1259
Fragments arranged by size:
1259 758 740 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 247
Size: 3830
BsiEI CG_ry'CG
Cuts at: 104 327 1372 1418 2626 104
Size: 223 1045 46 1208 1308
Fragments arranged by size:
1308 1208 1045 223 46
BsiHKAI G_wGCw'C
Cuts at: 607 844 1622 1812 2604 607
Size: 237 778 190 792 1833
Fragments arranged by size:
1833 792 778 237 190
Bsp1286I G_dGCh'C
Cuts at: 607 844 1455 1548 1622 1812 1874 2604 607
Size: 237 611 93 74 190 62 730 1833
Fragments arranged by size:
1833 730 611 237 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3050 3050
Size: 3830
BspHI T'CATG_A
Cuts at: 3006 3006
Size: 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 528 880 1094 1396 1777 528
Size: 352 214 302 381 2581
Fragments arranged by size:
2581 381 352 302 214
BsrBI CCG'CTC
Cuts at: 50 705 765 2122 2176 3660 3825 50
Size: 655 60 1357 54 1484 165 55
Fragments arranged by size:
1484 1357 655 165 60 55 54
BsrDI GCAATG_nn'
Cuts at: 743 1742 743
Size: 999 2831
BsrFI r'CCGG_y
Cuts at: 150 1828 2009 2218 150
Size: 1678 181 209 1762
Fragments arranged by size:
1762 1678 209 181
BssSI C'ACGA_G
Cuts at: 2101 2459 2101
Size: 358 3472
BstAPI GCAn_nnn'nTGC
Cuts at: 335 745 1207 335
Size: 410 462 2958
Fragments arranged by size:
2958 462 410
BstBI TT'CG_AA
Cuts at: 2191 2191
Size: 3830
BstXI CCAn_nnnn'nTGG
Cuts at: 219 219
Size: 3830
BstYI r'GATC_y
Cuts at: 1 855 1322 1345 1680 1926 2938 3024 3036
Size: 854 467 23 335 246 1012 86 12
Cuts at: 3036 3382 1
Size: 346 449
Fragments arranged by size:
1012 854 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 698 107
Size: 591 3239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 270 492 1780 1961 2124 270
Size: 222 1288 181 163 1976
Fragments arranged by size:
1976 1288 222 181 163
BtsI GCAGTG_nn'
Cuts at: 674 1073 674
Size: 399 3431
DraI TTT'AAA
Cuts at: 829 829
Size: 3830
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2394 1536
Size: 858 2972
EaeI y'GGCC_r
Cuts at: 221 695 1415 1589 1980 2007 3352 221
Size: 474 720 174 391 27 1345 699
Fragments arranged by size:
1345 720 699 474 391 174 27
EagI C'GGCC_G
Cuts at: 1415 1415
Size: 3830
EarI CTCTTCn'nnn_
Cuts at: 653 1853 2063 653
Size: 1200 210 2420
Fragments arranged by size:
2420 1200 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 271 721 2038 2348 2494 3640 271
Size: 450 1317 310 146 1146 461
Fragments arranged by size:
1317 1146 461 450 310 146
EcoICRI GAG'CTC
Cuts at: 605 842 605
Size: 237 3593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 246 699 1655 2087 2834 3102 161
Size: 85 453 956 432 747 268 889
Fragments arranged by size:
956 889 747 453 432 268 85
EcoNI CCTnn'n_nnAGG
Cuts at: 457 527 457
Size: 70 3760
EcoO109I rG'GnC_Cy
Cuts at: 278 519 969 278
Size: 241 450 3139
Fragments arranged by size:
3139 450 241
EcoRI G'AATT_C
Cuts at: 834 834
Size: 3830
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1849 1849
Size: 3830
FspI TGC'GCA
Cuts at: 1611 1611
Size: 3830
HaeII r_GCGC'y
Cuts at: 1264 1512 2534 3513 1264
Size: 248 1022 979 1581
Fragments arranged by size:
1581 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 713 745 1672 1702 1704 1734 713
Size: 32 927 30 2 30 2809
Fragments arranged by size:
2809 927 32 30 30 2
HincII GTy'rAC
Cuts at: 869 869
Size: 3830
KasI G'GCGC_C
Cuts at: 1260 1508 1260
Size: 248 3582
KpnI G_GTAC'C
Cuts at: 850 850
Size: 3830
MluI A'CGCG_T
Cuts at: 3263 3263
Size: 3830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2685 2501
Size: 184 3646
MscI TGG'CCA
Cuts at: 697 1591 697
Size: 894 2936
MslI CAynn'nnrTG
Cuts at: 217 223 1819 1946 217
Size: 6 1596 127 2101
Fragments arranged by size:
2101 1596 127 6
MspA1I CmG'CkG
Cuts at: 355 941 1255 1615 2628 2873 355
Size: 586 314 360 1013 245 1312
Fragments arranged by size:
1312 1013 586 360 314 245
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 3830
NarI GG'CG_CC
Cuts at: 1261 1509 1261
Size: 248 3582
NcoI C'CATG_G
Cuts at: 107 698 107
Size: 591 3239
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 3830
NspI r_CATG'y
Cuts at: 883 1383 2290 883
Size: 500 907 2423
Fragments arranged by size:
2423 907 500
PciI A'CATG_T
Cuts at: 2286 2286
Size: 3830
PfoI T'CCnGG_A
Cuts at: 598 598
Size: 3830
PmeI GTTT'AAAC
Cuts at: 829 829
Size: 3830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2969 3001 2969
Size: 32 3798
PpuMI rG'GwC_Cy
Cuts at: 278 519 278
Size: 241 3589
PstI C_TGCA'G
Cuts at: 877 1562 877
Size: 685 3145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3830
PvuII CAG'CTG
Cuts at: 355 1255 1615 355
Size: 900 360 2570
Fragments arranged by size:
2570 900 360
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 3830
SacI G_AGCT'C
Cuts at: 607 844 607
Size: 237 3593
SalI G'TCGA_C
Cuts at: 867 867
Size: 3830
SanDI GG'GwC_CC
Cuts at: 519 519
Size: 3830
SapI GCTCTTCn'nnn_
Cuts at: 1853 2063 1853
Size: 210 3620
SbfI CC_TGCA'GG
Cuts at: 877 877
Size: 3830
ScaI AGT'ACT
Cuts at: 773 3347 773
Size: 2574 1256
SfcI C'TryA_G
Cuts at: 33 873 1558 2551 2742 33
Size: 840 685 993 191 1121
Fragments arranged by size:
1121 993 840 685 191
SfoI GGC'GCC
Cuts at: 1262 1510 1262
Size: 248 3582
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3830
SmaI CCC'GGG
Cuts at: 852 852
Size: 3830
SmlI C'TyrA_G
Cuts at: 524 985 1900 2392 2654 524
Size: 461 915 492 262 1700
Fragments arranged by size:
1700 915 492 461 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3830
SphI G_CATG'C
Cuts at: 883 1383 883
Size: 500 3330
StyI C'CwwG_G
Cuts at: 107 653 698 964 1318 107
Size: 546 45 266 354 2619
Fragments arranged by size:
2619 546 354 266 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1413 1546 2005 2214 1413
Size: 133 459 209 3029
Fragments arranged by size:
3029 459 209 133
TatI w'GTAC_w
Cuts at: 542 771 3345 542
Size: 229 2574 1027
Fragments arranged by size:
2574 1027 229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 3347 3379 524
Size: 32 2791 32 975
Fragments arranged by size:
2791 975 32 32
Tth111I GACn'n_nGTC
Cuts at: 1627 1627
Size: 3830
XbaI T'CTAG_A
Cuts at: 861 861
Size: 3830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 152 1325 152
Size: 1173 2657
XmaI C'CCGG_G
Cuts at: 850 850
Size: 3830
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI