Restriction map for
pF1KB8262
(Circular) (Six-base) MAPSORT of: pF1KB8262.seq Check: 554 from: 1 to: 3686
pF1KB8262 3686 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1511 1943 2690 2958 1511
Size: 432 747 268 2239
Fragments arranged by size:
2239 747 432 268
AflIII A'CryG_T
Cuts at: 1038 2142 3119 1038
Size: 1104 977 1605
Fragments arranged by size:
1605 1104 977
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AhdI GACnn_n'nnGTC
Cuts at: 426 426
Size: 3686
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 151 183 151
Size: 32 3654
AlwNI CAG_nnn'CTG
Cuts at: 991 2558 991
Size: 1567 2119
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 690 3039 3295 690
Size: 2349 256 1081
Fragments arranged by size:
2349 1081 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 117 706 117
Size: 589 3097
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 702 1116 1364 1399 702
Size: 414 248 35 2989
Fragments arranged by size:
2989 414 248 35
BanII G_rGCy'C
Cuts at: 118 299 700 1730 118
Size: 181 401 1030 2074
Fragments arranged by size:
2074 1030 401 181
BbeI G_GCGC'C
Cuts at: 1120 1368 1120
Size: 248 3438
BbsI GAAGACnn'nnnn_
Cuts at: 327 491 327
Size: 164 3522
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 1206 1206
Size: 3686
BglII A'GATC_T
Cuts at: 1201 3238 1201
Size: 2037 1649
BlpI GC'TnA_GC
Cuts at: 798 798
Size: 3686
Bme1580I G_kGCm'C
Cuts at: 261 269 1311 1404 2460 261
Size: 8 1042 93 1056 1487
Fragments arranged by size:
1487 1056 1042 93 8
BmgBI CAC'GTC
Cuts at: 633 633
Size: 3686
BmrI ACTGGGnnnn_n'
Cuts at: 898 979 1314 3458 898
Size: 81 335 2144 1126
Fragments arranged by size:
2144 1126 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 632 598
Size: 34 3652
Bpu10I CC'TnA_GC
Cuts at: 364 490 364
Size: 126 3560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 862 1741 2233 2531 283
Size: 579 879 492 298 1438
Fragments arranged by size:
1438 879 579 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3390 3390
Size: 3686
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 1117 1365 2067 3563 1117
Size: 248 702 1496 1240
Fragments arranged by size:
1496 1240 702 248
BsaWI w'CCGG_w
Cuts at: 875 1396 2074 2348 2495 2906 875
Size: 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 622 652 622
Size: 30 3656
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 595 379
Size: 216 3470
BseYI C'CCAG_C
Cuts at: 348 1111 1869 2446 2942 348
Size: 763 758 577 496 1092
Fragments arranged by size:
1092 763 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 299 700 1478 1668 2460 299
Size: 401 778 190 792 1525
Fragments arranged by size:
1525 792 778 401 190
Bsp1286I G_dGCh'C
Cuts at: 118 261 269 299 700 1311 1404 1478 1668
Size: 143 8 30 401 611 93 74 190
Cuts at: 1668 1730 2460 118
Size: 62 730 1344
Fragments arranged by size:
1344 730 611 401 190 143 93 74
62 30 8
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 2862 2862
Size: 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 950 1252 1633 736
Size: 214 302 381 2789
Fragments arranged by size:
2789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1598 1598
Size: 3686
BsrFI r'CCGG_y
Cuts at: 494 1684 1865 2074 494
Size: 1190 181 209 2106
Fragments arranged by size:
2106 1190 209 181
BssSI C'ACGA_G
Cuts at: 1957 2315 1957
Size: 358 3328
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1063
Size: 3686
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstYI r'GATC_y
Cuts at: 1 185 384 711 1178 1201 1536 1782 2794
Size: 184 199 327 467 23 335 246 1012
Cuts at: 2794 2880 2892 3238 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 327 246 199
184 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1636 1817 1980 1636
Size: 181 163 3342
Fragments arranged by size:
3342 181 163
BtsI GCAGTG_nn'
Cuts at: 929 929
Size: 3686
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 146 1271 1445 1836 1863 3208 146
Size: 1125 174 391 27 1345 624
Fragments arranged by size:
1345 1125 624 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 1709 1919 1709
Size: 210 3476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1894 2204 2350 3496 1894
Size: 310 146 1146 2084
Fragments arranged by size:
2084 1146 310 146
EcoICRI GAG'CTC
Cuts at: 297 698 297
Size: 401 3285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 632 1511 1943 2690 2958 598
Size: 34 879 432 747 268 1326
Fragments arranged by size:
1326 879 747 432 268 34
EcoNI CCTnn'n_nnAGG
Cuts at: 133 133
Size: 3686
EcoO109I rG'GnC_Cy
Cuts at: 825 825
Size: 3686
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 379 1705 379
Size: 1326 2360
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 1120 1368 2390 3369 1120
Size: 248 1022 979 1437
Fragments arranged by size:
1437 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 412 444 1528 1558 1560 1590 412
Size: 32 1084 30 2 30 2508
Fragments arranged by size:
2508 1084 32 30 30 2
HincII GTy'rAC
Cuts at: 725 725
Size: 3686
KasI G'GCGC_C
Cuts at: 1116 1364 1116
Size: 248 3438
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 1447 1447
Size: 3686
MslI CAynn'nnrTG
Cuts at: 1675 1802 1675
Size: 127 3559
MspA1I CmG'CkG
Cuts at: 234 797 1111 1471 2484 2729 234
Size: 563 314 360 1013 245 1191
Fragments arranged by size:
1191 1013 563 360 314 245
NaeI GCC'GGC
Cuts at: 496 1867 496
Size: 1371 2315
NarI GG'CG_CC
Cuts at: 1117 1365 1117
Size: 248 3438
NcoI C'CATG_G
Cuts at: 107 107
Size: 3686
NgoMIV G'CCGG_C
Cuts at: 494 1865 494
Size: 1371 2315
NspI r_CATG'y
Cuts at: 739 1239 2146 739
Size: 500 907 2279
Fragments arranged by size:
2279 907 500
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PfoI T'CCnGG_A
Cuts at: 380 575 380
Size: 195 3491
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PstI C_TGCA'G
Cuts at: 331 733 1418 331
Size: 402 685 2599
Fragments arranged by size:
2599 685 402
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 1111 1471 1111
Size: 360 3326
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 299 700 299
Size: 401 3285
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 1709 1919 1709
Size: 210 3476
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 327 729 1414 2407 2598 33
Size: 294 402 685 993 191 1121
Fragments arranged by size:
1121 993 685 402 294 191
SfoI GGC'GCC
Cuts at: 1118 1366 1118
Size: 248 3438
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 298 841 1756 2248 2510 298
Size: 543 915 492 262 1474
Fragments arranged by size:
1474 915 543 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
StyI C'CwwG_G
Cuts at: 107 134 820 1174 107
Size: 27 686 354 2619
Fragments arranged by size:
2619 686 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1269 1402 1861 2070 1269
Size: 133 459 209 2885
Fragments arranged by size:
2885 459 209 133
TatI w'GTAC_w
Cuts at: 3201 3201
Size: 3686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 622 654 3203 3235 622
Size: 32 2549 32 1073
Fragments arranged by size:
2549 1073 32 32
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1181 141
Size: 1040 2646
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI