Restriction map for
pF1KB8263
(Circular) (Six-base) MAPSORT of: pF1KB8263.seq Check: 2687 from: 1 to: 4076
pF1KB8263 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 1092 1092
Size: 4076
AclI AA'CG_TT
Cuts at: 1070 3916 1070
Size: 2846 1230
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
AflIII A'CryG_T
Cuts at: 1428 2532 3509 1428
Size: 1104 977 1995
Fragments arranged by size:
1995 1104 977
AgeI A'CCGG_T
Cuts at: 2464 2464
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 1381 2948 1381
Size: 1567 2509
ApaLI G'TGCA_C
Cuts at: 2846 2846
Size: 4076
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 604 615 1096 604
Size: 11 481 3584
Fragments arranged by size:
3584 481 11
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 619 1092 1506 1754 1789 619
Size: 473 414 248 35 2906
Fragments arranged by size:
2906 473 414 248 35
BanII G_rGCy'C
Cuts at: 1090 2120 1090
Size: 1030 3046
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BbsI GAAGACnn'nnnn_
Cuts at: 387 387
Size: 4076
BbvCI CC'TCA_GC
Cuts at: 927 927
Size: 4076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 768 802 768
Size: 34 4042
BciVI GTATCCnnnnn_n'
Cuts at: 1974 2735 4076 1974
Size: 761 1341 1974
Fragments arranged by size:
1974 1341 761
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglI GCCn_nnn'nGGC
Cuts at: 155 191 381 155
Size: 36 190 3850
Fragments arranged by size:
3850 190 36
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 1188 1188
Size: 4076
Bme1580I G_kGCm'C
Cuts at: 622 1701 1794 2850 622
Size: 1079 93 1056 1848
Fragments arranged by size:
1848 1079 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1288 1369 1704 3848 1288
Size: 81 335 2144 1516
Fragments arranged by size:
2144 1516 335 81
BmtI G_CTAG'C
Cuts at: 541 541
Size: 4076
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4076
Bpu10I CC'TnA_GC
Cuts at: 590 927 590
Size: 337 3739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1252 2131 2623 2921 1024
Size: 228 879 492 298 2179
Fragments arranged by size:
2179 879 492 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 633 1013 3780 633
Size: 380 2767 929
Fragments arranged by size:
2767 929 380
BsaAI yAC'GTr
Cuts at: 1429 1429
Size: 4076
BsaBI GATnn'nnATC
Cuts at: 6 574 1590 3627 6
Size: 568 1016 2037 455
Fragments arranged by size:
2037 1016 568 455
BsaHI Gr'CG_yC
Cuts at: 1507 1755 2457 3953 1507
Size: 248 702 1496 1630
Fragments arranged by size:
1630 1496 702 248
BsaWI w'CCGG_w
Cuts at: 654 1265 1786 2464 2738 2885 3296 654
Size: 611 521 678 274 147 411 1434
Fragments arranged by size:
1434 678 611 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 257 260 287 290 257
Size: 3 27 3 4043
Fragments arranged by size:
4043 27 3 3
BseYI C'CCAG_C
Cuts at: 821 1501 2259 2836 3332 821
Size: 680 758 577 496 1565
Fragments arranged by size:
1565 758 680 577 496
BsiEI CG_ry'CG
Cuts at: 104 150 1618 1664 2872 104
Size: 46 1468 46 1208 1308
Fragments arranged by size:
1468 1308 1208 46 46
BsiHKAI G_wGCw'C
Cuts at: 1090 1868 2058 2850 1090
Size: 778 190 792 2316
Fragments arranged by size:
2316 792 778 190
BsmI GAATG_Cn'
Cuts at: 858 858
Size: 4076
Bsp1286I G_dGCh'C
Cuts at: 622 1090 1701 1794 1868 2058 2120 2850 622
Size: 468 611 93 74 190 62 730 1848
Fragments arranged by size:
1848 730 611 468 190 93 74 62
BspEI T'CCGG_A
Cuts at: 654 3296 654
Size: 2642 1434
BspHI T'CATG_A
Cuts at: 3252 3252
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1126 1340 1642 2023 1126
Size: 214 302 381 3179
Fragments arranged by size:
3179 381 302 214
BsrBI CCG'CTC
Cuts at: 50 530 692 2368 2422 3906 4071 50
Size: 480 162 1676 54 1484 165 55
Fragments arranged by size:
1676 1484 480 165 162 55 54
BsrDI GCAATG_nn'
Cuts at: 797 1988 797
Size: 1191 2885
BsrFI r'CCGG_y
Cuts at: 2074 2255 2464 2074
Size: 181 209 3686
Fragments arranged by size:
3686 209 181
BsrGI T'GTAC_A
Cuts at: 1043 1043
Size: 4076
BssSI C'ACGA_G
Cuts at: 2347 2705 2347
Size: 358 3718
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstEII G'GTnAC_C
Cuts at: 163 426 163
Size: 263 3813
BstXI CCAn_nnnn'nTGG
Cuts at: 307 438 694 307
Size: 131 256 3689
Fragments arranged by size:
3689 256 131
BstYI r'GATC_y
Cuts at: 1 569 1101 1568 1591 1926 2172 3184 3270
Size: 568 532 467 23 335 246 1012 86
Cuts at: 3270 3282 3628 1
Size: 12 346 449
Fragments arranged by size:
1012 568 532 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 129 168 623 1028 129
Size: 39 455 405 3177
Fragments arranged by size:
3177 455 405 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 771 2026 2207 2370 771
Size: 1255 181 163 2477
Fragments arranged by size:
2477 1255 181 163
BtsI GCAGTG_nn'
Cuts at: 1319 1319
Size: 4076
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DraIII CAC_nnn'GTG
Cuts at: 888 888
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 2640 1782
Size: 858 3218
EaeI y'GGCC_r
Cuts at: 147 696 716 1661 1835 2226 2253 3598 147
Size: 549 20 945 174 391 27 1345 625
Fragments arranged by size:
1345 945 625 549 391 174 27 20
EagI C'GGCC_G
Cuts at: 147 1661 147
Size: 1514 2562
EarI CTCTTCn'nnn_
Cuts at: 276 463 718 2099 2309 276
Size: 187 255 1381 210 2043
Fragments arranged by size:
2043 1381 255 210 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 233 2284 2594 2740 3886 233
Size: 2051 310 146 1146 423
Fragments arranged by size:
2051 1146 423 310 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 3080 3348 1901
Size: 432 747 268 2629
Fragments arranged by size:
2629 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1215 1215
Size: 4076
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 1510 1758 2780 3759 1510
Size: 248 1022 979 1827
Fragments arranged by size:
1827 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1918 1948 1950 1980 1918
Size: 30 2 30 4014
Fragments arranged by size:
4014 30 30 2
HincII GTy'rAC
Cuts at: 998 1115 998
Size: 117 3959
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 1096 1096
Size: 4076
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2747 2931 2747
Size: 184 3892
MscI TGG'CCA
Cuts at: 718 1837 718
Size: 1119 2957
MslI CAynn'nnrTG
Cuts at: 305 383 389 742 790 2065 2192 305
Size: 78 6 353 48 1275 127 2189
Fragments arranged by size:
2189 1275 353 127 78 48 6
MspA1I CmG'CkG
Cuts at: 1187 1501 1861 2874 3119 1187
Size: 314 360 1013 245 2144
Fragments arranged by size:
2144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 4076
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NdeI CA'TA_TG
Cuts at: 1011 1011
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 4076
NheI G'CTAG_C
Cuts at: 537 537
Size: 4076
NspI r_CATG'y
Cuts at: 783 1129 1629 2536 783
Size: 346 500 907 2323
Fragments arranged by size:
2323 907 500 346
PasI CC'CwG_GG
Cuts at: 445 445
Size: 4076
PciI A'CATG_T
Cuts at: 2532 2532
Size: 4076
PflMI CCAn_nnn'nTGG
Cuts at: 1029 1029
Size: 4076
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4044
PstI C_TGCA'G
Cuts at: 1123 1808 1123
Size: 685 3391
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 1501 1861 1501
Size: 360 3716
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SexAI A'CCwGG_T
Cuts at: 437 437
Size: 4076
SfcI C'TryA_G
Cuts at: 33 1119 1804 2797 2988 33
Size: 1086 685 993 191 1121
Fragments arranged by size:
1121 1086 993 685 191
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 1098 1098
Size: 4076
SmlI C'TyrA_G
Cuts at: 1003 1231 2146 2638 2900 1003
Size: 228 915 492 262 2179
Fragments arranged by size:
2179 915 492 262 228
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 783 1129 1629 783
Size: 346 500 3230
Fragments arranged by size:
3230 500 346
StyI C'CwwG_G
Cuts at: 1058 1210 1564 1058
Size: 152 354 3570
Fragments arranged by size:
3570 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 544 779 1659 1792 2251 2460 544
Size: 235 880 133 459 209 2160
Fragments arranged by size:
2160 880 459 235 209 133
TatI w'GTAC_w
Cuts at: 1043 3591 1043
Size: 2548 1528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 3593 3625 872
Size: 32 2689 32 1323
Fragments arranged by size:
2689 1323 32 32
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1571 1571
Size: 4076
XmaI C'CCGG_G
Cuts at: 1096 1096
Size: 4076
XmnI GAAnn'nnTTC
Cuts at: 523 523
Size: 4076
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NspI PasI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmgBI BpmI BsaXI
BsgI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI