Restriction map for
pF1KB8264
(Circular) (Six-base) MAPSORT of: pF1KB8264.seq Check: 7082 from: 1 to: 3743
pF1KB8264 3743 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 663 663
Size: 3743
AccI GT'mk_AC
Cuts at: 464 781 464
Size: 317 3426
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1568 2000 2747 3015 1568
Size: 432 747 268 2296
Fragments arranged by size:
2296 747 432 268
AflIII A'CryG_T
Cuts at: 1095 2199 3176 1095
Size: 1104 977 1662
Fragments arranged by size:
1662 1104 977
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AleI CACnn'nnGTG
Cuts at: 176 176
Size: 3743
AlwNI CAG_nnn'CTG
Cuts at: 216 459 498 510 1048 2615 216
Size: 243 39 12 538 1567 1344
Fragments arranged by size:
1567 1344 538 243 39 12
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 747 3096 3352 747
Size: 2349 256 1138
Fragments arranged by size:
2349 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 763 763
Size: 3743
BamHI G'GATC_C
Cuts at: 623 768 623
Size: 145 3598
BanI G'GyrC_C
Cuts at: 759 1173 1421 1456 759
Size: 414 248 35 3046
Fragments arranged by size:
3046 414 248 35
BanII G_rGCy'C
Cuts at: 347 414 757 1787 347
Size: 67 343 1030 2303
Fragments arranged by size:
2303 1030 343 67
BbeI G_GCGC'C
Cuts at: 1177 1425 1177
Size: 248 3495
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglII A'GATC_T
Cuts at: 1258 3295 1258
Size: 2037 1706
BlpI GC'TnA_GC
Cuts at: 855 855
Size: 3743
Bme1580I G_kGCm'C
Cuts at: 1368 1461 2517 1368
Size: 93 1056 2594
Fragments arranged by size:
2594 1056 93
BmgBI CAC'GTC
Cuts at: 552 552
Size: 3743
BmrI ACTGGGnnnn_n'
Cuts at: 689 955 1036 1371 3515 689
Size: 266 81 335 2144 917
Fragments arranged by size:
2144 917 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 3743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 508 238
Size: 270 3473
Bpu10I CC'TnA_GC
Cuts at: 545 545
Size: 3743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2290 2588 919
Size: 879 492 298 2074
Fragments arranged by size:
2074 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3447 3447
Size: 3743
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 1174 1422 2124 3620 1174
Size: 248 702 1496 1297
Fragments arranged by size:
1496 1297 702 248
BsaWI w'CCGG_w
Cuts at: 932 1453 2131 2405 2552 2963 932
Size: 521 678 274 147 411 1712
Fragments arranged by size:
1712 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 112
Size: 30 3713
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 136 235 355 409 583 133
Size: 3 99 120 54 174 3293
Fragments arranged by size:
3293 174 120 99 54 3
BseYI C'CCAG_C
Cuts at: 1168 1926 2503 2999 1168
Size: 758 577 496 1912
Fragments arranged by size:
1912 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 754 445
Size: 309 3434
BsiEI CG_ry'CG
Cuts at: 104 177 265 1285 1331 2539 104
Size: 73 88 1020 46 1208 1308
Fragments arranged by size:
1308 1208 1020 88 73 46
BsiHKAI G_wGCw'C
Cuts at: 533 551 757 1535 1725 2517 533
Size: 18 206 778 190 792 1759
Fragments arranged by size:
1759 792 778 206 190 18
BsmBI CGTCTCn'nnnn_
Cuts at: 118 694 118
Size: 576 3167
Bsp1286I G_dGCh'C
Cuts at: 347 414 533 551 757 1368 1461 1535 1725
Size: 67 119 18 206 611 93 74 190
Cuts at: 1725 1787 2517 347
Size: 62 730 1573
Fragments arranged by size:
1573 730 611 206 190 119 93 74
67 62 18
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 557 2919 557
Size: 2362 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 663 701 726 793 1007 1309 1690 663
Size: 38 25 67 214 302 381 2716
Fragments arranged by size:
2716 381 302 214 67 38 25
BsrBI CCG'CTC
Cuts at: 50 2035 2089 3573 3738 50
Size: 1985 54 1484 165 55
Fragments arranged by size:
1985 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1655 1655
Size: 3743
BsrFI r'CCGG_y
Cuts at: 131 725 1741 1922 2131 131
Size: 594 1016 181 209 1743
Fragments arranged by size:
1743 1016 594 209 181
BssHII G'CGCG_C
Cuts at: 646 646
Size: 3743
BssSI C'ACGA_G
Cuts at: 532 2014 2372 532
Size: 1482 358 1903
Fragments arranged by size:
1903 1482 358
BstAPI GCAn_nnn'nTGC
Cuts at: 498 510 1120 498
Size: 12 610 3121
Fragments arranged by size:
3121 610 12
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstEII G'GTnAC_C
Cuts at: 189 189
Size: 3743
BstXI CCAn_nnnn'nTGG
Cuts at: 178 178
Size: 3743
BstYI r'GATC_y
Cuts at: 1 623 768 1235 1258 1593 1839 2851 2937
Size: 622 145 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 622 467 449 346 335 246 145
86 23 12
BtgI C'CryG_G
Cuts at: 107 177 265 107
Size: 70 88 3585
Fragments arranged by size:
3585 88 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 986 986
Size: 3743
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2307 1449
Size: 858 2885
EaeI y'GGCC_r
Cuts at: 262 1328 1502 1893 1920 3265 262
Size: 1066 174 391 27 1345 740
Fragments arranged by size:
1345 1066 740 391 174 27
EagI C'GGCC_G
Cuts at: 262 1328 262
Size: 1066 2677
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 435 1951 2261 2407 3553 435
Size: 1516 310 146 1146 625
Fragments arranged by size:
1516 1146 625 310 146
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 508 1568 2000 2747 3015 238
Size: 270 1060 432 747 268 966
Fragments arranged by size:
1060 966 747 432 270 268
EcoO109I rG'GnC_Cy
Cuts at: 636 882 636
Size: 246 3497
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1762 1762
Size: 3743
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 1177 1425 2447 3426 1177
Size: 248 1022 979 1494
Fragments arranged by size:
1494 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 584 616 1585 1615 1617 1647 584
Size: 32 969 30 2 30 2680
Fragments arranged by size:
2680 969 32 30 30 2
HincII GTy'rAC
Cuts at: 465 782 465
Size: 317 3426
KasI G'GCGC_C
Cuts at: 1173 1421 1173
Size: 248 3495
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2598 2414
Size: 184 3559
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 176 302 370 421 1732 1859 176
Size: 126 68 51 1311 127 2060
Fragments arranged by size:
2060 1311 127 126 68 51
MspA1I CmG'CkG
Cuts at: 165 267 615 854 1168 1528 2541 2786 165
Size: 102 348 239 314 360 1013 245 1122
Fragments arranged by size:
1122 1013 360 348 314 245 239 102
NaeI GCC'GGC
Cuts at: 1924 1924
Size: 3743
NarI GG'CG_CC
Cuts at: 1174 1422 1174
Size: 248 3495
NcoI C'CATG_G
Cuts at: 107 107
Size: 3743
NgoMIV G'CCGG_C
Cuts at: 1922 1922
Size: 3743
NotI GC'GGCC_GC
Cuts at: 262 262
Size: 3743
NspI r_CATG'y
Cuts at: 390 796 1296 2203 390
Size: 406 500 907 1930
Fragments arranged by size:
1930 907 500 406
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PflMI CCAn_nnn'nTGG
Cuts at: 216 216
Size: 3743
PfoI T'CCnGG_A
Cuts at: 485 485
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2882 2914 2882
Size: 32 3711
PpuMI rG'GwC_Cy
Cuts at: 636 636
Size: 3743
PstI C_TGCA'G
Cuts at: 503 790 1475 503
Size: 287 685 2771
Fragments arranged by size:
2771 685 287
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 165 615 1168 1528 165
Size: 450 553 360 2380
Fragments arranged by size:
2380 553 450 360
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SacII CC_GC'GG
Cuts at: 268 268
Size: 3743
SalI G'TCGA_C
Cuts at: 463 780 463
Size: 317 3426
SanDI GG'GwC_CC
Cuts at: 636 636
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 499 786 1471 2464 2655 33
Size: 466 287 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 287 191
SfoI GGC'GCC
Cuts at: 1175 1423 1175
Size: 248 3495
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 765 765
Size: 3743
SmlI C'TyrA_G
Cuts at: 898 1813 2305 2567 898
Size: 915 492 262 2074
Fragments arranged by size:
2074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 390 796 1296 390
Size: 406 500 2837
Fragments arranged by size:
2837 500 406
StyI C'CwwG_G
Cuts at: 107 347 563 877 1231 107
Size: 240 216 314 354 2619
Fragments arranged by size:
2619 354 314 240 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1326 1459 1918 2127 1326
Size: 133 459 209 2942
Fragments arranged by size:
2942 459 209 133
TatI w'GTAC_w
Cuts at: 374 479 3258 374
Size: 105 2779 859
Fragments arranged by size:
2779 859 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 651 683 3260 3292 651
Size: 32 2577 32 1102
Fragments arranged by size:
2577 1102 32 32
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 456 483 1238 456
Size: 27 755 2961
Fragments arranged by size:
2961 755 27
XmaI C'CCGG_G
Cuts at: 763 763
Size: 3743
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BsiWI BsmI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI