Restriction map for
pF1KB8265
(Circular) (Six-base) MAPSORT of: pF1KB8265.seq Check: 783 from: 1 to: 4466
pF1KB8265 4466 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 311 719 1504 311
Size: 408 785 3273
Fragments arranged by size:
3273 785 408
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 487 2291 2723 3470 3738 249
Size: 238 1804 432 747 268 977
Fragments arranged by size:
1804 977 747 432 268 238
AflIII A'CryG_T
Cuts at: 1818 2922 3899 1818
Size: 1104 977 2385
Fragments arranged by size:
2385 1104 977
AgeI A'CCGG_T
Cuts at: 496 2854 496
Size: 2358 2108
AleI CACnn'nnGTG
Cuts at: 1192 1192
Size: 4466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 4466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 179
Size: 32 4434
AlwNI CAG_nnn'CTG
Cuts at: 1771 3338 1771
Size: 1567 2899
ApaLI G'TGCA_C
Cuts at: 492 3236 492
Size: 2744 1722
ApoI r'AATT_y
Cuts at: 470 868 1470 3819 4075 470
Size: 398 602 2349 256 861
Fragments arranged by size:
2349 861 602 398 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 296 419 1486 296
Size: 123 1067 3276
Fragments arranged by size:
3276 1067 123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1320 1353 1320
Size: 33 4433
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 349 627 885 1145 1194 1482 1896 2144 2179
Size: 278 258 260 49 288 414 248 35
Cuts at: 2179 349
Size: 2636
Fragments arranged by size:
2636 414 288 278 260 258 248 49
35
BanII G_rGCy'C
Cuts at: 176 659 849 1275 1480 2510 176
Size: 483 190 426 205 1030 2132
Fragments arranged by size:
2132 1030 483 426 205 190
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 493 493
Size: 4466
BbvCI CC'TCA_GC
Cuts at: 1262 1262
Size: 4466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4432
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3125 4466 2364
Size: 761 1341 2364
Fragments arranged by size:
2364 1341 761
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BglI GCCn_nnn'nGGC
Cuts at: 1154 1154
Size: 4466
BglII A'GATC_T
Cuts at: 1981 4018 1981
Size: 2037 2429
BlpI GC'TnA_GC
Cuts at: 1240 1578 1240
Size: 338 4128
Bme1580I G_kGCm'C
Cuts at: 296 428 496 890 1150 2091 2184 3240 296
Size: 132 68 394 260 941 93 1056 1522
Fragments arranged by size:
1522 1056 941 394 260 132 93 68
BmgBI CAC'GTC
Cuts at: 1074 1074
Size: 4466
BmrI ACTGGGnnnn_n'
Cuts at: 1678 1759 2094 4238 1678
Size: 81 335 2144 1906
Fragments arranged by size:
2144 1906 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 138 2833 138
Size: 2695 1771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 712 628
Size: 84 4382
Bpu10I CC'TnA_GC
Cuts at: 374 1262 374
Size: 888 3578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 1642 2521 3013 3311 1411
Size: 231 879 492 298 2566
Fragments arranged by size:
2566 879 492 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 1199 4170 1199
Size: 2971 1495
BsaAI yAC'GTr
Cuts at: 291 1332 1819 291
Size: 1041 487 2938
Fragments arranged by size:
2938 1041 487
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 1897 2145 2847 4343 1897
Size: 248 702 1496 2020
Fragments arranged by size:
2020 1496 702 248
BsaWI w'CCGG_w
Cuts at: 496 1655 2176 2854 3128 3275 3686 496
Size: 1159 521 678 274 147 411 1276
Fragments arranged by size:
1276 1159 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 254 1420 254
Size: 1166 3300
BseYI C'CCAG_C
Cuts at: 167 1891 2649 3226 3722 167
Size: 1724 758 577 496 911
Fragments arranged by size:
1724 911 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2008 2054 3262 104
Size: 1904 46 1208 1308
Fragments arranged by size:
1904 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 176 496 698 1480 2258 2448 3240 176
Size: 320 202 782 778 190 792 1402
Fragments arranged by size:
1402 792 782 778 320 202 190
BsmBI CGTCTCn'nnnn_
Cuts at: 265 580 265
Size: 315 4151
Bsp1286I G_dGCh'C
Cuts at: 176 296 428 496 659 698 849 890 1150
Size: 120 132 68 163 39 151 41 260
Cuts at: 1150 1275 1480 2091 2184 2258 2448 2510 3240
Size: 125 205 611 93 74 190 62 730
Cuts at: 3240 176
Size: 1402
Fragments arranged by size:
1402 730 611 260 205 190 163 151
132 125 120 93 74 68 62 41
39
BspEI T'CCGG_A
Cuts at: 3686 3686
Size: 4466
BspHI T'CATG_A
Cuts at: 3642 3642
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1516 1730 2032 2413 1516
Size: 214 302 381 3569
Fragments arranged by size:
3569 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2758 2812 4296 4461 50
Size: 2708 54 1484 165 55
Fragments arranged by size:
2708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 165 1100 1256 2378 165
Size: 935 156 1122 2253
Fragments arranged by size:
2253 1122 935 156
BsrFI r'CCGG_y
Cuts at: 496 2464 2645 2854 496
Size: 1968 181 209 2108
Fragments arranged by size:
2108 1968 209 181
BssSI C'ACGA_G
Cuts at: 2737 3095 2737
Size: 358 4108
BstAPI GCAn_nnn'nTGC
Cuts at: 164 1843 164
Size: 1679 2787
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 205 529 1011 205
Size: 324 482 3660
Fragments arranged by size:
3660 482 324
BstYI r'GATC_y
Cuts at: 1 1491 1958 1981 2316 2562 3574 3660 3672
Size: 1490 467 23 335 246 1012 86 12
Cuts at: 3672 4018 1
Size: 346 449
Fragments arranged by size:
1490 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1032 1065 1032
Size: 33 4433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 821 1709 821
Size: 888 3578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 312 347 1239 1274 312
Size: 35 892 35 3504
Fragments arranged by size:
3504 892 35 35
DraI TTT'AAA
Cuts at: 1465 1465
Size: 4466
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3030 2172
Size: 858 3608
EaeI y'GGCC_r
Cuts at: 135 207 914 1211 2051 2225 2616 2643 3988
Size: 72 707 297 840 174 391 27 1345
Cuts at: 3988 135
Size: 613
Fragments arranged by size:
1345 840 707 613 391 297 174 72
27
EagI C'GGCC_G
Cuts at: 2051 2051
Size: 4466
EarI CTCTTCn'nnn_
Cuts at: 370 2489 2699 370
Size: 2119 210 2137
Fragments arranged by size:
2137 2119 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2674 2984 3130 4276 2674
Size: 310 146 1146 2864
Fragments arranged by size:
2864 1146 310 146
EcoICRI GAG'CTC
Cuts at: 174 1478 174
Size: 1304 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 487 628 712 2291 2723 3470 3738 249
Size: 238 141 84 1579 432 747 268 977
Fragments arranged by size:
1579 977 747 432 268 238 141 84
EcoNI CCTnn'n_nnAGG
Cuts at: 1213 1393 1213
Size: 180 4286
EcoO109I rG'GnC_Cy
Cuts at: 1605 1605
Size: 4466
EcoRI G'AATT_C
Cuts at: 470 868 1470 470
Size: 398 602 3466
Fragments arranged by size:
3466 602 398
EcoRV GAT'ATC
Cuts at: 762 762
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1332 2485 1332
Size: 1153 3313
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 281 771 1900 2148 3170 4149 281
Size: 490 1129 248 1022 979 598
Fragments arranged by size:
1129 1022 979 598 490 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2308 2338 2340 2370 2308
Size: 30 2 30 4404
Fragments arranged by size:
4404 30 30 2
HincII GTy'rAC
Cuts at: 858 1505 858
Size: 647 3819
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 3137 3321 443
Size: 2694 184 1588
Fragments arranged by size:
2694 1588 184
MscI TGG'CCA
Cuts at: 137 209 916 1213 2227 137
Size: 72 707 297 1014 2376
Fragments arranged by size:
2376 1014 707 297 72
MslI CAynn'nnrTG
Cuts at: 1192 2455 2582 1192
Size: 1263 127 3076
Fragments arranged by size:
3076 1263 127
MspA1I CmG'CkG
Cuts at: 646 1577 1891 2251 3264 3509 646
Size: 931 314 360 1013 245 1603
Fragments arranged by size:
1603 1013 931 360 314 245
NaeI GCC'GGC
Cuts at: 2647 2647
Size: 4466
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NcoI C'CATG_G
Cuts at: 1032 1032
Size: 4466
NdeI CA'TA_TG
Cuts at: 921 921
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 2645 2645
Size: 4466
NspI r_CATG'y
Cuts at: 1239 1519 2019 2926 1239
Size: 280 500 907 2779
Fragments arranged by size:
2779 907 500 280
PciI A'CATG_T
Cuts at: 2922 2922
Size: 4466
PflMI CCAn_nnn'nTGG
Cuts at: 1137 1137
Size: 4466
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PmlI CAC'GTG
Cuts at: 291 291
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4434
PshAI GACnn'nnGTC
Cuts at: 743 743
Size: 4466
PstI C_TGCA'G
Cuts at: 1513 2198 1513
Size: 685 3781
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 646 1891 2251 646
Size: 1245 360 2861
Fragments arranged by size:
2861 1245 360
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 176 1480 176
Size: 1304 3162
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 370 2489 2699 370
Size: 2119 210 2137
Fragments arranged by size:
2137 2119 210
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SexAI A'CCwGG_T
Cuts at: 881 881
Size: 4466
SfcI C'TryA_G
Cuts at: 33 647 968 1509 2194 3187 3378 33
Size: 614 321 541 685 993 191 1121
Fragments arranged by size:
1121 993 685 614 541 321 191
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 298 1488 298
Size: 1190 3276
SmlI C'TyrA_G
Cuts at: 1390 1621 2536 3028 3290 1390
Size: 231 915 492 262 2566
Fragments arranged by size:
2566 915 492 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1239 1519 2019 1239
Size: 280 500 3686
Fragments arranged by size:
3686 500 280
StuI AGG'CCT
Cuts at: 1267 1267
Size: 4466
StyI C'CwwG_G
Cuts at: 1032 1214 1600 1954 1032
Size: 182 386 354 3544
Fragments arranged by size:
3544 386 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 482 694 1324 2049 2182 2641 2850 482
Size: 212 630 725 133 459 209 2098
Fragments arranged by size:
2098 725 630 459 212 209 133
TatI w'GTAC_w
Cuts at: 3981 3981
Size: 4466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4434
Tth111I GACn'n_nGTC
Cuts at: 2263 2263
Size: 4466
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 529 1961 529
Size: 1432 3034
XmaI C'CCGG_G
Cuts at: 296 1486 296
Size: 1190 3276
XmnI GAAnn'nnTTC
Cuts at: 543 543
Size: 4466
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BfrBI BmtI BsaXI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII FseI FspAI HindIII
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PfoI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI