Restriction map for
pF1KB8267
(Circular) (Six-base) MAPSORT of: pF1KB8267.seq Check: 4815 from: 1 to: 3806
pF1KB8267 3806 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 844 844
Size: 3806
Acc65I G'GTAC_C
Cuts at: 822 822
Size: 3806
AclI AA'CG_TT
Cuts at: 3646 3646
Size: 3806
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1631 2063 2810 3078 1631
Size: 432 747 268 2359
Fragments arranged by size:
2359 747 432 268
AflIII A'CryG_T
Cuts at: 1158 2262 3239 1158
Size: 1104 977 1725
Fragments arranged by size:
1725 1104 977
AgeI A'CCGG_T
Cuts at: 2194 2194
Size: 3806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 151 148
Size: 3 3803
AlwNI CAG_nnn'CTG
Cuts at: 565 574 1111 2678 565
Size: 9 537 1567 1693
Fragments arranged by size:
1693 1567 537 9
ApaI G_GGCC'C
Cuts at: 418 679 418
Size: 261 3545
ApaLI G'TGCA_C
Cuts at: 2576 2576
Size: 3806
ApoI r'AATT_y
Cuts at: 222 810 3159 3415 222
Size: 588 2349 256 613
Fragments arranged by size:
2349 613 588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3806
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
AvaI C'yCGr_G
Cuts at: 386 826 386
Size: 440 3366
BamHI G'GATC_C
Cuts at: 719 831 719
Size: 112 3694
BanI G'GyrC_C
Cuts at: 193 822 1236 1484 1519 193
Size: 629 414 248 35 2480
Fragments arranged by size:
2480 629 414 248 35
BanII G_rGCy'C
Cuts at: 418 600 679 820 1850 418
Size: 182 79 141 1030 2374
Fragments arranged by size:
2374 1030 182 141 79
BbeI G_GCGC'C
Cuts at: 1240 1488 1240
Size: 248 3558
BciVI GTATCCnnnnn_n'
Cuts at: 1704 2465 3806 1704
Size: 761 1341 1704
Fragments arranged by size:
1704 1341 761
BclI T'GATC_A
Cuts at: 1326 1326
Size: 3806
BglII A'GATC_T
Cuts at: 1321 3358 1321
Size: 2037 1769
BlpI GC'TnA_GC
Cuts at: 918 918
Size: 3806
Bme1580I G_kGCm'C
Cuts at: 418 679 1431 1524 2580 418
Size: 261 752 93 1056 1644
Fragments arranged by size:
1644 1056 752 261 93
BmrI ACTGGGnnnn_n'
Cuts at: 600 1018 1099 1434 3578 600
Size: 418 81 335 2144 828
Fragments arranged by size:
2144 828 418 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 3806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 647 422
Size: 225 3581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1861 2353 2651 982
Size: 879 492 298 2137
Fragments arranged by size:
2137 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 345 687 3510 345
Size: 342 2823 641
Fragments arranged by size:
2823 641 342
BsaAI yAC'GTr
Cuts at: 1159 1159
Size: 3806
BsaBI GATnn'nnATC
Cuts at: 6 1320 3357 6
Size: 1314 2037 455
Fragments arranged by size:
2037 1314 455
BsaHI Gr'CG_yC
Cuts at: 1237 1485 2187 3683 1237
Size: 248 702 1496 1360
Fragments arranged by size:
1496 1360 702 248
BsaWI w'CCGG_w
Cuts at: 995 1516 2194 2468 2615 3026 995
Size: 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 353 665 764 353
Size: 312 99 3395
Fragments arranged by size:
3395 312 99
BseYI C'CCAG_C
Cuts at: 450 454 490 1231 1989 2566 3062 450
Size: 4 36 741 758 577 496 1194
Fragments arranged by size:
1194 758 741 577 496 36 4
BsiEI CG_ry'CG
Cuts at: 104 180 1348 1394 2602 104
Size: 76 1168 46 1208 1308
Fragments arranged by size:
1308 1208 1168 76 46
BsiHKAI G_wGCw'C
Cuts at: 657 820 1598 1788 2580 657
Size: 163 778 190 792 1883
Fragments arranged by size:
1883 792 778 190 163
BsmI GAATG_Cn'
Cuts at: 770 770
Size: 3806
Bsp1286I G_dGCh'C
Cuts at: 418 600 657 679 820 1431 1524 1598 1788
Size: 182 57 22 141 611 93 74 190
Cuts at: 1788 1850 2580 418
Size: 62 730 1644
Fragments arranged by size:
1644 730 611 190 182 141 93 74
62 57 22
BspEI T'CCGG_A
Cuts at: 3026 3026
Size: 3806
BspHI T'CATG_A
Cuts at: 2982 2982
Size: 3806
BspMI ACCTGCnnnn'nnnn_
Cuts at: 856 1070 1372 1753 856
Size: 214 302 381 2909
Fragments arranged by size:
2909 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2098 2152 3636 3801 50
Size: 2048 54 1484 165 55
Fragments arranged by size:
2048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1718 1718
Size: 3806
BsrFI r'CCGG_y
Cuts at: 1804 1985 2194 1804
Size: 181 209 3416
Fragments arranged by size:
3416 209 181
BssSI C'ACGA_G
Cuts at: 2077 2435 2077
Size: 358 3448
BstAPI GCAn_nnn'nTGC
Cuts at: 487 565 574 1183 487
Size: 78 9 609 3110
Fragments arranged by size:
3110 609 78 9
BstBI TT'CG_AA
Cuts at: 2167 2167
Size: 3806
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3806
BstYI r'GATC_y
Cuts at: 1 182 719 831 1298 1321 1656 1902 2914
Size: 181 537 112 467 23 335 246 1012
Cuts at: 2914 3000 3012 3358 1
Size: 86 12 346 449
Fragments arranged by size:
1012 537 467 449 346 335 246 181
112 86 23 12
BtgI C'CryG_G
Cuts at: 501 621 501
Size: 120 3686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1756 1937 2100 1756
Size: 181 163 3462
Fragments arranged by size:
3462 181 163
BtsI GCAGTG_nn'
Cuts at: 1049 1049
Size: 3806
DraI TTT'AAA
Cuts at: 805 805
Size: 3806
DrdI GACnn_nn'nnGTC
Cuts at: 1512 2370 1512
Size: 858 2948
EaeI y'GGCC_r
Cuts at: 177 618 1391 1565 1956 1983 3328 177
Size: 441 773 174 391 27 1345 655
Fragments arranged by size:
1345 773 655 441 391 174 27
EagI C'GGCC_G
Cuts at: 177 1391 177
Size: 1214 2592
EarI CTCTTCn'nnn_
Cuts at: 287 540 726 1829 2039 287
Size: 253 186 1103 210 2054
Fragments arranged by size:
2054 1103 253 210 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2014 2324 2470 3616 2014
Size: 310 146 1146 2204
Fragments arranged by size:
2204 1146 310 146
EcoICRI GAG'CTC
Cuts at: 818 818
Size: 3806
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 647 1631 2063 2810 3078 422
Size: 225 984 432 747 268 1150
Fragments arranged by size:
1150 984 747 432 268 225
EcoNI CCTnn'n_nnAGG
Cuts at: 731 731
Size: 3806
EcoO109I rG'GnC_Cy
Cuts at: 358 414 628 706 945 358
Size: 56 214 78 239 3219
Fragments arranged by size:
3219 239 214 78 56
EcoRI G'AATT_C
Cuts at: 810 810
Size: 3806
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1825 1825
Size: 3806
FspI TGC'GCA
Cuts at: 1587 1587
Size: 3806
HaeII r_GCGC'y
Cuts at: 1240 1488 2510 3489 1240
Size: 248 1022 979 1557
Fragments arranged by size:
1557 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1648 1678 1680 1710 1648
Size: 30 2 30 3744
Fragments arranged by size:
3744 30 30 2
HincII GTy'rAC
Cuts at: 845 845
Size: 3806
KasI G'GCGC_C
Cuts at: 1236 1484 1236
Size: 248 3558
KpnI G_GTAC'C
Cuts at: 826 826
Size: 3806
MluI A'CGCG_T
Cuts at: 3239 3239
Size: 3806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 625 2477 2661 625
Size: 1852 184 1770
Fragments arranged by size:
1852 1770 184
MscI TGG'CCA
Cuts at: 1567 1567
Size: 3806
MslI CAynn'nnrTG
Cuts at: 112 1795 1922 112
Size: 1683 127 1996
Fragments arranged by size:
1996 1683 127
MspA1I CmG'CkG
Cuts at: 454 565 571 917 1231 1591 2604 2849 454
Size: 111 6 346 314 360 1013 245 1411
Fragments arranged by size:
1411 1013 360 346 314 245 111 6
NaeI GCC'GGC
Cuts at: 1987 1987
Size: 3806
NarI GG'CG_CC
Cuts at: 1237 1485 1237
Size: 248 3558
NgoMIV G'CCGG_C
Cuts at: 1985 1985
Size: 3806
NspI r_CATG'y
Cuts at: 753 859 1359 2266 753
Size: 106 500 907 2293
Fragments arranged by size:
2293 907 500 106
PasI CC'CwG_GG
Cuts at: 418 781 418
Size: 363 3443
PciI A'CATG_T
Cuts at: 2262 2262
Size: 3806
PflMI CCAn_nnn'nTGG
Cuts at: 400 502 685 400
Size: 102 183 3521
Fragments arranged by size:
3521 183 102
PfoI T'CCnGG_A
Cuts at: 759 759
Size: 3806
PmeI GTTT'AAAC
Cuts at: 805 805
Size: 3806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2945 2977 2945
Size: 32 3774
PpuMI rG'GwC_Cy
Cuts at: 358 628 706 358
Size: 270 78 3458
Fragments arranged by size:
3458 270 78
PspOMI G'GGCC_C
Cuts at: 414 675 414
Size: 261 3545
PspXI vC'TCGA_Gb
Cuts at: 386 386
Size: 3806
PstI C_TGCA'G
Cuts at: 140 173 483 570 853 1538 140
Size: 33 310 87 283 685 2408
Fragments arranged by size:
2408 685 310 283 87 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3806
PvuII CAG'CTG
Cuts at: 454 571 1231 1591 454
Size: 117 660 360 2669
Fragments arranged by size:
2669 660 360 117
RsrII CG'GwC_CG
Cuts at: 2001 2001
Size: 3806
SacI G_AGCT'C
Cuts at: 820 820
Size: 3806
SalI G'TCGA_C
Cuts at: 843 843
Size: 3806
SanDI GG'GwC_CC
Cuts at: 706 706
Size: 3806
SapI GCTCTTCn'nnn_
Cuts at: 1829 2039 1829
Size: 210 3596
SbfI CC_TGCA'GG
Cuts at: 173 483 853 173
Size: 310 370 3126
Fragments arranged by size:
3126 370 310
ScaI AGT'ACT
Cuts at: 3323 3323
Size: 3806
SfcI C'TryA_G
Cuts at: 33 136 169 479 566 849 1534 2527 2718
Size: 103 33 310 87 283 685 993 191
Cuts at: 2718 33
Size: 1121
Fragments arranged by size:
1121 993 685 310 283 191 103 87
33
SfoI GGC'GCC
Cuts at: 1238 1486 1238
Size: 248 3558
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3806
SmaI CCC'GGG
Cuts at: 828 828
Size: 3806
SmlI C'TyrA_G
Cuts at: 386 961 1876 2368 2630 386
Size: 575 915 492 262 1562
Fragments arranged by size:
1562 915 575 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3806
SphI G_CATG'C
Cuts at: 753 859 1359 753
Size: 106 500 3200
Fragments arranged by size:
3200 500 106
StuI AGG'CCT
Cuts at: 794 794
Size: 3806
StyI C'CwwG_G
Cuts at: 940 1294 940
Size: 354 3452
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 1389 1522 1981 2190 298
Size: 1091 133 459 209 1914
Fragments arranged by size:
1914 1091 459 209 133
TatI w'GTAC_w
Cuts at: 3321 3321
Size: 3806
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 3774
Tth111I GACn'n_nGTC
Cuts at: 1603 1603
Size: 3806
XbaI T'CTAG_A
Cuts at: 837 837
Size: 3806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 613 1301 613
Size: 688 3118
XhoI C'TCGA_G
Cuts at: 386 386
Size: 3806
XmaI C'CCGG_G
Cuts at: 826 826
Size: 3806
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI