Restriction map for
pF1KB8269
(Circular) (Six-base) MAPSORT of: pF1KB8269.seq Check: 5615 from: 1 to: 3716
pF1KB8269 3716 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 624 1541 1973 2720 2988 624
Size: 917 432 747 268 1352
Fragments arranged by size:
1352 917 747 432 268
AfeI AGC'GCT
Cuts at: 449 449
Size: 3716
AflIII A'CryG_T
Cuts at: 1068 2172 3149 1068
Size: 1104 977 1635
Fragments arranged by size:
1635 1104 977
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 1021 2588 1021
Size: 1567 2149
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 736 736
Size: 3716
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 511 732 1146 1394 1429 511
Size: 221 414 248 35 2798
Fragments arranged by size:
2798 414 248 221 35
BanII G_rGCy'C
Cuts at: 608 709 730 1760 608
Size: 101 21 1030 2564
Fragments arranged by size:
2564 1030 101 21
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbsI GAAGACnn'nnnn_
Cuts at: 373 373
Size: 3716
BciVI GTATCCnnnnn_n'
Cuts at: 136 541 1614 2375 3716 136
Size: 405 1073 761 1341 136
Fragments arranged by size:
1341 1073 761 405 136
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 1231 3268 1231
Size: 2037 1679
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 1341 1434 2490 1341
Size: 93 1056 2567
Fragments arranged by size:
2567 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 592 619 278
Size: 314 27 3375
Fragments arranged by size:
3375 314 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 565 1069 565
Size: 504 3212
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 251 281 251
Size: 30 3686
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 616 403
Size: 213 3503
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 162 540 1258 1304 2512 104
Size: 58 378 718 46 1208 1308
Fragments arranged by size:
1308 1208 718 378 58 46
BsiHKAI G_wGCw'C
Cuts at: 608 730 1508 1698 2490 608
Size: 122 778 190 792 1834
Fragments arranged by size:
1834 792 778 190 122
Bsp1286I G_dGCh'C
Cuts at: 608 709 730 1341 1434 1508 1698 1760 2490
Size: 101 21 611 93 74 190 62 730
Cuts at: 2490 608
Size: 1834
Fragments arranged by size:
1834 730 611 190 101 93 74 62
21
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 201 2892 201
Size: 2691 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BsrGI T'GTAC_A
Cuts at: 267 267
Size: 3716
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 648 2077 648
Size: 1429 2287
BstXI CCAn_nnnn'nTGG
Cuts at: 508 508
Size: 3716
BstYI r'GATC_y
Cuts at: 1 741 1208 1231 1566 1812 2824 2910 2922
Size: 740 467 23 335 246 1012 86 12
Cuts at: 2922 3268 1
Size: 346 449
Fragments arranged by size:
1012 740 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 586 586
Size: 3716
BtgI C'CryG_G
Cuts at: 107 338 501 107
Size: 231 163 3322
Fragments arranged by size:
3322 231 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 581 959 581
Size: 378 3338
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 167 479 1301 1475 1866 1893 3238 167
Size: 312 822 174 391 27 1345 645
Fragments arranged by size:
1345 822 645 391 312 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 606 728 606
Size: 122 3594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 592 619 624 1541 1973 2720 2988 278
Size: 314 27 5 917 432 747 268 1006
Fragments arranged by size:
1006 917 747 432 314 268 27 5
EcoO109I rG'GnC_Cy
Cuts at: 133 486 855 133
Size: 353 369 2994
Fragments arranged by size:
2994 369 353
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 451 1150 1398 2420 3399 451
Size: 699 248 1022 979 768
Fragments arranged by size:
1022 979 768 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 1558 1588 1590 1620 384
Size: 32 1142 30 2 30 2480
Fragments arranged by size:
2480 1142 32 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 182 228 370 2387 2571 182
Size: 46 142 2017 184 1327
Fragments arranged by size:
2017 1327 184 142 46
MscI TGG'CCA
Cuts at: 481 1477 481
Size: 996 2720
MslI CAynn'nnrTG
Cuts at: 506 1705 1832 506
Size: 1199 127 2390
Fragments arranged by size:
2390 1199 127
MspA1I CmG'CkG
Cuts at: 827 1141 1501 2514 2759 827
Size: 314 360 1013 245 1784
Fragments arranged by size:
1784 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 107 107
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NspI r_CATG'y
Cuts at: 769 1269 2176 769
Size: 500 907 2309
Fragments arranged by size:
2309 907 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PflMI CCAn_nnn'nTGG
Cuts at: 678 678
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PmlI CAC'GTG
Cuts at: 565 565
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PsiI TTA'TAA
Cuts at: 308 308
Size: 3716
PstI C_TGCA'G
Cuts at: 763 1448 763
Size: 685 3031
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 608 730 608
Size: 122 3594
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 759 1444 2437 2628 33
Size: 726 685 993 191 1121
Fragments arranged by size:
1121 993 726 685 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
SspI AAT'ATT
Cuts at: 180 180
Size: 3716
StyI C'CwwG_G
Cuts at: 107 129 476 482 850 1204 107
Size: 22 347 6 368 354 2619
Fragments arranged by size:
2619 368 354 347 22 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 554 1299 1432 1891 2100 554
Size: 745 133 459 209 2170
Fragments arranged by size:
2170 745 459 209 133
TatI w'GTAC_w
Cuts at: 267 3231 267
Size: 2964 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
XmnI GAAnn'nnTTC
Cuts at: 284 616 284
Size: 332 3384
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI