Restriction map for
pF1KB8272
(Circular) (Six-base) MAPSORT of: pF1KB8272.seq Check: 4225 from: 1 to: 3596
pF1KB8272 3596 bp
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 470 470
Size: 3596
AccI GT'mk_AC
Cuts at: 634 634
Size: 3596
Acc65I G'GTAC_C
Cuts at: 612 612
Size: 3596
AclI AA'CG_TT
Cuts at: 3436 3436
Size: 3596
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 1421 1853 2600 2868 208
Size: 1213 432 747 268 936
Fragments arranged by size:
1213 936 747 432 268
AflIII A'CryG_T
Cuts at: 113 948 2052 3029 113
Size: 835 1104 977 680
Fragments arranged by size:
1104 977 835 680
AgeI A'CCGG_T
Cuts at: 1984 1984
Size: 3596
AlwNI CAG_nnn'CTG
Cuts at: 237 901 2468 237
Size: 664 1567 1365
Fragments arranged by size:
1567 1365 664
ApaLI G'TGCA_C
Cuts at: 457 2366 457
Size: 1909 1687
ApoI r'AATT_y
Cuts at: 600 2949 3205 600
Size: 2349 256 991
Fragments arranged by size:
2349 991 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
AvaI C'yCGr_G
Cuts at: 429 616 429
Size: 187 3409
BamHI G'GATC_C
Cuts at: 621 621
Size: 3596
BanI G'GyrC_C
Cuts at: 131 612 1026 1274 1309 131
Size: 481 414 248 35 2418
Fragments arranged by size:
2418 481 414 248 35
BanII G_rGCy'C
Cuts at: 610 1640 610
Size: 1030 2566
BbeI G_GCGC'C
Cuts at: 135 1030 1278 135
Size: 895 248 2453
Fragments arranged by size:
2453 895 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 162 196 162
Size: 34 3562
BciVI GTATCCnnnnn_n'
Cuts at: 1494 2255 3596 1494
Size: 761 1341 1494
Fragments arranged by size:
1494 1341 761
BclI T'GATC_A
Cuts at: 1116 1116
Size: 3596
BglI GCCn_nnn'nGGC
Cuts at: 346 346
Size: 3596
BglII A'GATC_T
Cuts at: 1111 3148 1111
Size: 2037 1559
BlpI GC'TnA_GC
Cuts at: 708 708
Size: 3596
Bme1580I G_kGCm'C
Cuts at: 461 1221 1314 2370 461
Size: 760 93 1056 1687
Fragments arranged by size:
1687 1056 760 93
BmgBI CAC'GTC
Cuts at: 114 114
Size: 3596
BmrI ACTGGGnnnn_n'
Cuts at: 286 808 889 1224 3368 286
Size: 522 81 335 2144 514
Fragments arranged by size:
2144 522 514 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 528 528
Size: 3596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 772 1651 2143 2441 196
Size: 576 879 492 298 1351
Fragments arranged by size:
1351 879 576 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3300 3300
Size: 3596
BsaAI yAC'GTr
Cuts at: 949 949
Size: 3596
BsaBI GATnn'nnATC
Cuts at: 6 1110 3147 6
Size: 1104 2037 455
Fragments arranged by size:
2037 1104 455
BsaHI Gr'CG_yC
Cuts at: 132 158 1027 1275 1977 3473 132
Size: 26 869 248 702 1496 255
Fragments arranged by size:
1496 869 702 255 248 26
BsaWI w'CCGG_w
Cuts at: 785 1306 1984 2258 2405 2816 785
Size: 521 678 274 147 411 1565
Fragments arranged by size:
1565 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 501 531 501
Size: 30 3566
BseYI C'CCAG_C
Cuts at: 321 581 1021 1779 2356 2852 321
Size: 260 440 758 577 496 1065
Fragments arranged by size:
1065 758 577 496 440 260
BsiEI CG_ry'CG
Cuts at: 104 1138 1184 2392 104
Size: 1034 46 1208 1308
Fragments arranged by size:
1308 1208 1034 46
BsiHKAI G_wGCw'C
Cuts at: 461 610 1388 1578 2370 461
Size: 149 778 190 792 1687
Fragments arranged by size:
1687 792 778 190 149
Bsp1286I G_dGCh'C
Cuts at: 461 610 1221 1314 1388 1578 1640 2370 461
Size: 149 611 93 74 190 62 730 1687
Fragments arranged by size:
1687 730 611 190 149 93 74 62
BspEI T'CCGG_A
Cuts at: 2816 2816
Size: 3596
BspHI T'CATG_A
Cuts at: 122 2772 122
Size: 2650 946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 470 646 860 1162 1543 470
Size: 176 214 302 381 2523
Fragments arranged by size:
2523 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 1888 1942 3426 3591 50
Size: 1838 54 1484 165 55
Fragments arranged by size:
1838 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1508 1508
Size: 3596
BsrFI r'CCGG_y
Cuts at: 1594 1775 1984 1594
Size: 181 209 3206
Fragments arranged by size:
3206 209 181
BsrGI T'GTAC_A
Cuts at: 239 239
Size: 3596
BssSI C'ACGA_G
Cuts at: 1867 2225 1867
Size: 358 3238
BstAPI GCAn_nnn'nTGC
Cuts at: 973 973
Size: 3596
BstBI TT'CG_AA
Cuts at: 182 1957 182
Size: 1775 1821
BstXI CCAn_nnnn'nTGG
Cuts at: 520 520
Size: 3596
BstYI r'GATC_y
Cuts at: 1 621 1088 1111 1446 1692 2704 2790 2802
Size: 620 467 23 335 246 1012 86 12
Cuts at: 2802 3148 1
Size: 346 449
Fragments arranged by size:
1012 620 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1546 1727 1890 1546
Size: 181 163 3252
Fragments arranged by size:
3252 181 163
BtsI GCAGTG_nn'
Cuts at: 444 839 444
Size: 395 3201
DraI TTT'AAA
Cuts at: 595 595
Size: 3596
DrdI GACnn_nn'nnGTC
Cuts at: 1302 2160 1302
Size: 858 2738
EaeI y'GGCC_r
Cuts at: 310 485 1181 1355 1746 1773 3118 310
Size: 175 696 174 391 27 1345 788
Fragments arranged by size:
1345 788 696 391 175 174 27
EagI C'GGCC_G
Cuts at: 1181 1181
Size: 3596
EarI CTCTTCn'nnn_
Cuts at: 1619 1829 1619
Size: 210 3386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1804 2114 2260 3406 1804
Size: 310 146 1146 1994
Fragments arranged by size:
1994 1146 310 146
EcoICRI GAG'CTC
Cuts at: 608 608
Size: 3596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 528 1421 1853 2600 2868 208
Size: 320 893 432 747 268 936
Fragments arranged by size:
936 893 747 432 320 268
EcoO109I rG'GnC_Cy
Cuts at: 318 366 735 318
Size: 48 369 3179
Fragments arranged by size:
3179 369 48
EcoRI G'AATT_C
Cuts at: 600 600
Size: 3596
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1615 1615
Size: 3596
FspI TGC'GCA
Cuts at: 1377 1377
Size: 3596
HaeII r_GCGC'y
Cuts at: 135 1030 1278 2300 3279 135
Size: 895 248 1022 979 452
Fragments arranged by size:
1022 979 895 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 1438 1468 1470 1500 501
Size: 32 905 30 2 30 2597
Fragments arranged by size:
2597 905 32 30 30 2
HincII GTy'rAC
Cuts at: 635 635
Size: 3596
KasI G'GCGC_C
Cuts at: 131 1026 1274 131
Size: 895 248 2453
Fragments arranged by size:
2453 895 248
KpnI G_GTAC'C
Cuts at: 616 616
Size: 3596
MluI A'CGCG_T
Cuts at: 3029 3029
Size: 3596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2267 2451 2267
Size: 184 3412
MscI TGG'CCA
Cuts at: 487 1357 487
Size: 870 2726
MslI CAynn'nnrTG
Cuts at: 112 464 1585 1712 112
Size: 352 1121 127 1996
Fragments arranged by size:
1996 1121 352 127
MspA1I CmG'CkG
Cuts at: 234 707 1021 1381 2394 2639 234
Size: 473 314 360 1013 245 1191
Fragments arranged by size:
1191 1013 473 360 314 245
NaeI GCC'GGC
Cuts at: 1777 1777
Size: 3596
NarI GG'CG_CC
Cuts at: 132 1027 1275 132
Size: 895 248 2453
Fragments arranged by size:
2453 895 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3596
NgoMIV G'CCGG_C
Cuts at: 1775 1775
Size: 3596
NspI r_CATG'y
Cuts at: 469 536 649 1149 2056 469
Size: 67 113 500 907 2009
Fragments arranged by size:
2009 907 500 113 67
PciI A'CATG_T
Cuts at: 2052 2052
Size: 3596
PflMI CCAn_nnn'nTGG
Cuts at: 258 483 258
Size: 225 3371
PmeI GTTT'AAAC
Cuts at: 595 595
Size: 3596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2735 2767 2735
Size: 32 3564
PstI C_TGCA'G
Cuts at: 643 1328 643
Size: 685 2911
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3596
PvuII CAG'CTG
Cuts at: 1021 1381 1021
Size: 360 3236
RsrII CG'GwC_CG
Cuts at: 1791 1791
Size: 3596
SacI G_AGCT'C
Cuts at: 610 610
Size: 3596
SalI G'TCGA_C
Cuts at: 633 633
Size: 3596
SapI GCTCTTCn'nnn_
Cuts at: 1619 1829 1619
Size: 210 3386
SbfI CC_TGCA'GG
Cuts at: 643 643
Size: 3596
ScaI AGT'ACT
Cuts at: 3113 3113
Size: 3596
SfcI C'TryA_G
Cuts at: 33 639 1324 2317 2508 33
Size: 606 685 993 191 1121
Fragments arranged by size:
1121 993 685 606 191
SfoI GGC'GCC
Cuts at: 133 1028 1276 133
Size: 895 248 2453
Fragments arranged by size:
2453 895 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3596
SmaI CCC'GGG
Cuts at: 618 618
Size: 3596
SmlI C'TyrA_G
Cuts at: 211 751 1666 2158 2420 211
Size: 540 915 492 262 1387
Fragments arranged by size:
1387 915 540 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3596
SphI G_CATG'C
Cuts at: 649 1149 649
Size: 500 3096
StyI C'CwwG_G
Cuts at: 107 161 730 1084 107
Size: 54 569 354 2619
Fragments arranged by size:
2619 569 354 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 590 1179 1312 1771 1980 590
Size: 589 133 459 209 2206
Fragments arranged by size:
2206 589 459 209 133
TatI w'GTAC_w
Cuts at: 239 3111 239
Size: 2872 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 3564
Tth111I GACn'n_nGTC
Cuts at: 1393 1393
Size: 3596
XbaI T'CTAG_A
Cuts at: 627 627
Size: 3596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 333 1091 333
Size: 758 2838
XmaI C'CCGG_G
Cuts at: 616 616
Size: 3596
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI