Restriction map for
pF1KB8275
(Circular) (Six-base) MAPSORT of: pF1KB8275.seq Check: 7836 from: 1 to: 4031
pF1KB8275 4031 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 479 1069 479
Size: 590 3441
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 3871 3871
Size: 4031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 466 511 710 1856 2288 3035 3303 389
Size: 77 45 199 1146 432 747 268 1117
Fragments arranged by size:
1146 1117 747 432 268 199 77 45
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 106 138 106
Size: 32 3999
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 463 656 1035 3384 3640 463
Size: 193 379 2349 256 854
Fragments arranged by size:
2349 854 379 256 193
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 1051 1051
Size: 4031
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 271 1047 1461 1709 1744 271
Size: 776 414 248 35 2558
Fragments arranged by size:
2558 776 414 248 35
BanII G_rGCy'C
Cuts at: 746 945 1045 2075 746
Size: 199 100 1030 2702
Fragments arranged by size:
2702 1030 199 100
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BciVI GTATCCnnnnn_n'
Cuts at: 996 1929 2690 4031 996
Size: 933 761 1341 996
Fragments arranged by size:
1341 996 933 761
BclI T'GATC_A
Cuts at: 1551 1551
Size: 4031
BglI GCCn_nnn'nGGC
Cuts at: 199 199
Size: 4031
BglII A'GATC_T
Cuts at: 441 1546 3583 441
Size: 1105 2037 889
Fragments arranged by size:
2037 1105 889
BlpI GC'TnA_GC
Cuts at: 1143 1143
Size: 4031
Bme1580I G_kGCm'C
Cuts at: 1656 1749 2805 1656
Size: 93 1056 2882
Fragments arranged by size:
2882 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1243 1324 1659 3803 1243
Size: 81 335 2144 1471
Fragments arranged by size:
2144 1471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 662 287
Size: 375 3656
Bpu10I CC'TnA_GC
Cuts at: 418 418
Size: 4031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 314 1462 1710 2412 3908 314
Size: 1148 248 702 1496 437
Fragments arranged by size:
1496 1148 702 437 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 182 212 106
Size: 30 46 30 3925
Fragments arranged by size:
3925 46 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 342 343 938 130
Size: 212 1 595 3223
Fragments arranged by size:
3223 595 212 1
BseYI C'CCAG_C
Cuts at: 422 1456 2214 2791 3287 422
Size: 1034 758 577 496 1166
Fragments arranged by size:
1166 1034 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 468
Size: 4031
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 746 945 1045 1823 2013 2805 746
Size: 199 100 778 190 792 1972
Fragments arranged by size:
1972 792 778 199 190 100
Bsp1286I G_dGCh'C
Cuts at: 746 945 1045 1656 1749 1823 2013 2075 2805
Size: 199 100 611 93 74 190 62 730
Cuts at: 2805 746
Size: 1972
Fragments arranged by size:
1972 730 611 199 190 100 93 74
62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 1295 1597 1978 1081
Size: 214 302 381 3134
Fragments arranged by size:
3134 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 645 2029 2210 2419 645
Size: 1384 181 209 2257
Fragments arranged by size:
2257 1384 209 181
BssSI C'ACGA_G
Cuts at: 944 2302 2660 944
Size: 1358 358 2315
Fragments arranged by size:
2315 1358 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstEII G'GTnAC_C
Cuts at: 958 958
Size: 4031
BstXI CCAn_nnnn'nTGG
Cuts at: 847 970 847
Size: 123 3908
BstYI r'GATC_y
Cuts at: 1 441 498 916 1056 1523 1546 1881 2127
Size: 440 57 418 140 467 23 335 246
Cuts at: 2127 3139 3225 3237 3583 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 440 418 346 335 246
140 86 57 23 12
BtgI C'CryG_G
Cuts at: 194 749 194
Size: 555 3476
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
DraI TTT'AAA
Cuts at: 543 1030 543
Size: 487 3544
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 1616 1790 2181 2208 3553 1616
Size: 174 391 27 1345 2094
Fragments arranged by size:
2094 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 686 933 945 2054 2264 686
Size: 247 12 1109 210 2453
Fragments arranged by size:
2453 1109 247 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 269 2239 2549 2695 3841 269
Size: 1970 310 146 1146 459
Fragments arranged by size:
1970 1146 459 310 146
EcoICRI GAG'CTC
Cuts at: 744 943 1043 744
Size: 199 100 3732
Fragments arranged by size:
3732 199 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 389 466 511 662 710 1856 2288 3035
Size: 102 77 45 151 48 1146 432 747
Cuts at: 3035 3303 287
Size: 268 1015
Fragments arranged by size:
1146 1015 747 432 268 151 102 77
48 45
EcoNI CCTnn'n_nnAGG
Cuts at: 506 506
Size: 4031
EcoO109I rG'GnC_Cy
Cuts at: 1170 1170
Size: 4031
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 2050 471
Size: 1579 2452
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 290 1465 1713 2735 3714 290
Size: 1175 248 1022 979 607
Fragments arranged by size:
1175 1022 979 607 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 718 750 1873 1903 1905 1935 718
Size: 32 1123 30 2 30 2814
Fragments arranged by size:
2814 1123 32 30 30 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 275 2702 2886 92
Size: 183 2427 184 1237
Fragments arranged by size:
2427 1237 184 183
MscI TGG'CCA
Cuts at: 1792 1792
Size: 4031
MslI CAynn'nnrTG
Cuts at: 2020 2147 2020
Size: 127 3904
MspA1I CmG'CkG
Cuts at: 148 220 387 1142 1456 1816 2829 3074 148
Size: 72 167 755 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 755 360 314 245 167 72
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 194 749 194
Size: 555 3476
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 1084 1584 2491 1084
Size: 500 907 2624
Fragments arranged by size:
2624 907 500
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 106 138 3170 3202 106
Size: 32 3032 32 935
Fragments arranged by size:
3032 935 32 32
PstI C_TGCA'G
Cuts at: 1078 1763 1078
Size: 685 3346
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 1456 1816 1456
Size: 360 3671
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 746 945 1045 746
Size: 199 100 3732
Fragments arranged by size:
3732 199 100
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 933 2054 2264 933
Size: 1121 210 2700
Fragments arranged by size:
2700 1121 210
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 709 3548 709
Size: 2839 1192
SfcI C'TryA_G
Cuts at: 33 1074 1759 2752 2943 33
Size: 1041 685 993 191 1121
Fragments arranged by size:
1121 1041 993 685 191
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 1186 2101 2593 2855 1186
Size: 915 492 262 2362
Fragments arranged by size:
2362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StuI AGG'CCT
Cuts at: 416 416
Size: 4031
StyI C'CwwG_G
Cuts at: 194 749 1165 1519 194
Size: 555 416 354 2706
Fragments arranged by size:
2706 555 416 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 707 3546 707
Size: 2839 1192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 182 214 3548 3580 182
Size: 32 3334 32 633
Fragments arranged by size:
3334 633 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 919 1062 919
Size: 143 3888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI