Restriction map for
pF1KB8276
(Circular) (Six-base) MAPSORT of: pF1KB8276.seq Check: 6283 from: 1 to: 3530
pF1KB8276 3530 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 568 568
Size: 3530
Acc65I G'GTAC_C
Cuts at: 546 546
Size: 3530
AclI AA'CG_TT
Cuts at: 3370 3370
Size: 3530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1355 1787 2534 2802 148
Size: 1207 432 747 268 876
Fragments arranged by size:
1207 876 747 432 268
AflIII A'CryG_T
Cuts at: 882 1986 2963 882
Size: 1104 977 1449
Fragments arranged by size:
1449 1104 977
AgeI A'CCGG_T
Cuts at: 1918 1918
Size: 3530
AlwNI CAG_nnn'CTG
Cuts at: 835 2402 835
Size: 1567 1963
ApaLI G'TGCA_C
Cuts at: 2300 2300
Size: 3530
ApoI r'AATT_y
Cuts at: 534 2883 3139 534
Size: 2349 256 925
Fragments arranged by size:
2349 925 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3530
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3530
AvaI C'yCGr_G
Cuts at: 277 468 550 277
Size: 191 82 3257
Fragments arranged by size:
3257 191 82
BamHI G'GATC_C
Cuts at: 464 555 464
Size: 91 3439
BanI G'GyrC_C
Cuts at: 546 960 1208 1243 546
Size: 414 248 35 2833
Fragments arranged by size:
2833 414 248 35
BanII G_rGCy'C
Cuts at: 544 1574 544
Size: 1030 2500
BbeI G_GCGC'C
Cuts at: 964 1212 964
Size: 248 3282
BbsI GAAGACnn'nnnn_
Cuts at: 136 136
Size: 3530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3496
BciVI GTATCCnnnnn_n'
Cuts at: 1428 2189 3530 1428
Size: 761 1341 1428
Fragments arranged by size:
1428 1341 761
BclI T'GATC_A
Cuts at: 1050 1050
Size: 3530
BglI GCCn_nnn'nGGC
Cuts at: 380 380
Size: 3530
BglII A'GATC_T
Cuts at: 1045 3082 1045
Size: 2037 1493
BlpI GC'TnA_GC
Cuts at: 642 642
Size: 3530
Bme1580I G_kGCm'C
Cuts at: 475 1155 1248 2304 475
Size: 680 93 1056 1701
Fragments arranged by size:
1701 1056 680 93
BmgBI CAC'GTC
Cuts at: 245 245
Size: 3530
BmrI ACTGGGnnnn_n'
Cuts at: 742 823 1158 3302 742
Size: 81 335 2144 970
Fragments arranged by size:
2144 970 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1897 1897
Size: 3530
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1585 2077 2375 706
Size: 879 492 298 1861
Fragments arranged by size:
1861 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3234 3234
Size: 3530
BsaAI yAC'GTr
Cuts at: 883 883
Size: 3530
BsaBI GATnn'nnATC
Cuts at: 6 1044 3081 6
Size: 1038 2037 455
Fragments arranged by size:
2037 1038 455
BsaHI Gr'CG_yC
Cuts at: 961 1209 1911 3407 961
Size: 248 702 1496 1084
Fragments arranged by size:
1496 1084 702 248
BsaWI w'CCGG_w
Cuts at: 719 1240 1918 2192 2339 2750 719
Size: 521 678 274 147 411 1499
Fragments arranged by size:
1499 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 364
Size: 3530
BseYI C'CCAG_C
Cuts at: 955 1713 2290 2786 955
Size: 758 577 496 1699
Fragments arranged by size:
1699 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 225
Size: 3530
BsiEI CG_ry'CG
Cuts at: 104 375 1072 1118 2326 104
Size: 271 697 46 1208 1308
Fragments arranged by size:
1308 1208 697 271 46
BsiHKAI G_wGCw'C
Cuts at: 544 1322 1512 2304 544
Size: 778 190 792 1770
Fragments arranged by size:
1770 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 169 169
Size: 3530
Bsp1286I G_dGCh'C
Cuts at: 475 544 1155 1248 1322 1512 1574 2304 475
Size: 69 611 93 74 190 62 730 1701
Fragments arranged by size:
1701 730 611 190 93 74 69 62
BspEI T'CCGG_A
Cuts at: 2750 2750
Size: 3530
BspHI T'CATG_A
Cuts at: 2706 2706
Size: 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 320 580 794 1096 1477 233
Size: 87 260 214 302 381 2286
Fragments arranged by size:
2286 381 302 260 214 87
BsrBI CCG'CTC
Cuts at: 50 1822 1876 3360 3525 50
Size: 1772 54 1484 165 55
Fragments arranged by size:
1772 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1442 1442
Size: 3530
BsrFI r'CCGG_y
Cuts at: 1528 1709 1918 1528
Size: 181 209 3140
Fragments arranged by size:
3140 209 181
BssSI C'ACGA_G
Cuts at: 1801 2159 1801
Size: 358 3172
BstAPI GCAn_nnn'nTGC
Cuts at: 907 907
Size: 3530
BstBI TT'CG_AA
Cuts at: 1891 1891
Size: 3530
BstXI CCAn_nnnn'nTGG
Cuts at: 156 156
Size: 3530
BstYI r'GATC_y
Cuts at: 1 464 555 1022 1045 1380 1626 2638 2724
Size: 463 91 467 23 335 246 1012 86
Cuts at: 2724 2736 3082 1
Size: 12 346 449
Fragments arranged by size:
1012 467 463 449 346 335 246 91
86 23 12
BtgI C'CryG_G
Cuts at: 291 369 375 411 291
Size: 78 6 36 3410
Fragments arranged by size:
3410 78 36 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1480 1661 1824 1480
Size: 181 163 3186
Fragments arranged by size:
3186 181 163
BtsI GCAGTG_nn'
Cuts at: 121 773 121
Size: 652 2878
DraI TTT'AAA
Cuts at: 529 529
Size: 3530
DraIII CAC_nnn'GTG
Cuts at: 195 195
Size: 3530
DrdI GACnn_nn'nnGTC
Cuts at: 1236 2094 1236
Size: 858 2672
EaeI y'GGCC_r
Cuts at: 359 372 429 447 1115 1289 1680 1707 3052
Size: 13 57 18 668 174 391 27 1345
Cuts at: 3052 359
Size: 837
Fragments arranged by size:
1345 837 668 391 174 57 27 18
13
EagI C'GGCC_G
Cuts at: 372 1115 372
Size: 743 2787
EarI CTCTTCn'nnn_
Cuts at: 1553 1763 1553
Size: 210 3320
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 285 1738 2048 2194 3340 285
Size: 1453 310 146 1146 475
Fragments arranged by size:
1453 1146 475 310 146
EcoICRI GAG'CTC
Cuts at: 542 542
Size: 3530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1355 1787 2534 2802 148
Size: 1207 432 747 268 876
Fragments arranged by size:
1207 876 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 669 669
Size: 3530
EcoRI G'AATT_C
Cuts at: 534 534
Size: 3530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1549 1549
Size: 3530
FspI TGC'GCA
Cuts at: 316 1311 316
Size: 995 2535
HaeII r_GCGC'y
Cuts at: 428 964 1212 2234 3213 428
Size: 536 248 1022 979 745
Fragments arranged by size:
1022 979 745 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 221 253 449 481 1372 1402 1404 1434 221
Size: 32 196 32 891 30 2 30 2317
Fragments arranged by size:
2317 891 196 32 32 30 30 2
HincII GTy'rAC
Cuts at: 569 569
Size: 3530
KasI G'GCGC_C
Cuts at: 960 1208 960
Size: 248 3282
KpnI G_GTAC'C
Cuts at: 550 550
Size: 3530
MluI A'CGCG_T
Cuts at: 2963 2963
Size: 3530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2385 2201
Size: 184 3346
MscI TGG'CCA
Cuts at: 361 1291 361
Size: 930 2600
MslI CAynn'nnrTG
Cuts at: 154 337 1519 1646 154
Size: 183 1182 127 2038
Fragments arranged by size:
2038 1182 183 127
MspA1I CmG'CkG
Cuts at: 293 371 377 413 641 955 1315 2328 2573
Size: 78 6 36 228 314 360 1013 245
Cuts at: 2573 293
Size: 1250
Fragments arranged by size:
1250 1013 360 314 245 228 78 36
6
NaeI GCC'GGC
Cuts at: 1711 1711
Size: 3530
NarI GG'CG_CC
Cuts at: 961 1209 961
Size: 248 3282
NgoMIV G'CCGG_C
Cuts at: 1709 1709
Size: 3530
NotI GC'GGCC_GC
Cuts at: 372 372
Size: 3530
NspI r_CATG'y
Cuts at: 583 1083 1990 583
Size: 500 907 2123
Fragments arranged by size:
2123 907 500
PciI A'CATG_T
Cuts at: 1986 1986
Size: 3530
PmeI GTTT'AAAC
Cuts at: 529 529
Size: 3530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 3498
PstI C_TGCA'G
Cuts at: 389 577 1262 389
Size: 188 685 2657
Fragments arranged by size:
2657 685 188
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3530
PvuII CAG'CTG
Cuts at: 955 1315 955
Size: 360 3170
RsrII CG'GwC_CG
Cuts at: 1725 1725
Size: 3530
SacI G_AGCT'C
Cuts at: 544 544
Size: 3530
SacII CC_GC'GG
Cuts at: 294 372 378 414 294
Size: 78 6 36 3410
Fragments arranged by size:
3410 78 36 6
SalI G'TCGA_C
Cuts at: 567 567
Size: 3530
SapI GCTCTTCn'nnn_
Cuts at: 1553 1763 1553
Size: 210 3320
SbfI CC_TGCA'GG
Cuts at: 577 577
Size: 3530
ScaI AGT'ACT
Cuts at: 3047 3047
Size: 3530
SexAI A'CCwGG_T
Cuts at: 191 191
Size: 3530
SfcI C'TryA_G
Cuts at: 33 385 573 1258 2251 2442 33
Size: 352 188 685 993 191 1121
Fragments arranged by size:
1121 993 685 352 191 188
SfiI GGCCn_nnn'nGGCC
Cuts at: 380 380
Size: 3530
SfoI GGC'GCC
Cuts at: 962 1210 962
Size: 248 3282
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3530
SmaI CCC'GGG
Cuts at: 470 552 470
Size: 82 3448
SmlI C'TyrA_G
Cuts at: 685 1600 2092 2354 685
Size: 915 492 262 1861
Fragments arranged by size:
1861 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3530
SphI G_CATG'C
Cuts at: 583 1083 583
Size: 500 3030
StuI AGG'CCT
Cuts at: 383 383
Size: 3530
StyI C'CwwG_G
Cuts at: 362 664 1018 362
Size: 302 354 2874
Fragments arranged by size:
2874 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1113 1246 1705 1914 1113
Size: 133 459 209 2729
Fragments arranged by size:
2729 459 209 133
TatI w'GTAC_w
Cuts at: 3045 3045
Size: 3530
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 3047 3079 179
Size: 32 2836 32 630
Fragments arranged by size:
2836 630 32 32
Tth111I GACn'n_nGTC
Cuts at: 340 1327 340
Size: 987 2543
XbaI T'CTAG_A
Cuts at: 561 561
Size: 3530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1025 1025
Size: 3530
XmaI C'CCGG_G
Cuts at: 468 550 468
Size: 82 3448
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI