Restriction map for
pF1KB8277
(Circular) (Six-base) MAPSORT of: pF1KB8277.seq Check: 4386 from: 1 to: 4184
pF1KB8277 4184 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 486 486
Size: 4184
AccI GT'mk_AC
Cuts at: 166 599 1222 166
Size: 433 623 3128
Fragments arranged by size:
3128 623 433
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 2009 2441 3188 3456 442
Size: 1567 432 747 268 1170
Fragments arranged by size:
1567 1170 747 432 268
AfeI AGC'GCT
Cuts at: 1151 1151
Size: 4184
AflIII A'CryG_T
Cuts at: 878 1058 1536 2640 3617 878
Size: 180 478 1104 977 1445
Fragments arranged by size:
1445 1104 977 478 180
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 773 774 805 806 773
Size: 1 31 1 4151
Fragments arranged by size:
4151 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 579 1489 3056 579
Size: 910 1567 1707
Fragments arranged by size:
1707 1567 910
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 1078 1188 3537 3793 1078
Size: 110 2349 256 1469
Fragments arranged by size:
2349 1469 256 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 4184
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 746 1204 746
Size: 458 3726
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 846 879 846
Size: 33 4151
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 1200 1614 1862 1897 1200
Size: 414 248 35 3487
Fragments arranged by size:
3487 414 248 35
BanII G_rGCy'C
Cuts at: 1198 2228 1198
Size: 1030 3154
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 478 478
Size: 4184
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 207 1025 1704 207
Size: 818 679 2687
Fragments arranged by size:
2687 818 679
BglII A'GATC_T
Cuts at: 1699 3736 1699
Size: 2037 2147
BlpI GC'TnA_GC
Cuts at: 193 266 1296 193
Size: 73 1030 3081
Fragments arranged by size:
3081 1030 73
Bme1580I G_kGCm'C
Cuts at: 830 1809 1902 2958 830
Size: 979 93 1056 2056
Fragments arranged by size:
2056 1056 979 93
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BmtI G_CTAG'C
Cuts at: 124 124
Size: 4184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 726 2551 726
Size: 1825 2359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 546 646 785 820 466
Size: 80 100 139 35 3830
Fragments arranged by size:
3830 139 100 80 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2239 2731 3029 1360
Size: 879 492 298 2515
Fragments arranged by size:
2515 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 905 1044 3888 905
Size: 139 2844 1201
Fragments arranged by size:
2844 1201 139
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 805 1030 1698 3735 6
Size: 799 225 668 2037 455
Fragments arranged by size:
2037 799 668 455 225
BsaHI Gr'CG_yC
Cuts at: 1615 1863 2565 4061 1615
Size: 248 702 1496 1738
Fragments arranged by size:
1738 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1163 1193 1163
Size: 30 4154
BseYI C'CCAG_C
Cuts at: 139 522 1609 2367 2944 3440 139
Size: 383 1087 758 577 496 883
Fragments arranged by size:
1087 883 758 577 496 383
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1198 1976 2166 2958 1198
Size: 778 190 792 2424
Fragments arranged by size:
2424 792 778 190
BsmI GAATG_Cn'
Cuts at: 947 947
Size: 4184
BsmBI CGTCTCn'nnnn_
Cuts at: 128 442 128
Size: 314 3870
Bsp1286I G_dGCh'C
Cuts at: 830 1198 1809 1902 1976 2166 2228 2958 830
Size: 368 611 93 74 190 62 730 2056
Fragments arranged by size:
2056 730 611 368 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 809 3360 809
Size: 2551 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 486 1234 1448 1750 2131 486
Size: 748 214 302 381 2539
Fragments arranged by size:
2539 748 381 302 214
BsrBI CCG'CTC
Cuts at: 50 270 819 2476 2530 4014 4179 50
Size: 220 549 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 549 220 165 55 54
BsrDI GCAATG_nn'
Cuts at: 360 2096 360
Size: 1736 2448
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BsrGI T'GTAC_A
Cuts at: 611 611
Size: 4184
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstEII G'GTnAC_C
Cuts at: 321 321
Size: 4184
BstXI CCAn_nnnn'nTGG
Cuts at: 312 312
Size: 4184
BstYI r'GATC_y
Cuts at: 1 750 1209 1676 1699 2034 2280 3292 3378
Size: 749 459 467 23 335 246 1012 86
Cuts at: 3378 3390 3736 1
Size: 12 346 449
Fragments arranged by size:
1012 749 467 459 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1053 1427 1053
Size: 374 3810
DraI TTT'AAA
Cuts at: 1183 1183
Size: 4184
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 438 1769 1943 2334 2361 3706 438
Size: 1331 174 391 27 1345 916
Fragments arranged by size:
1345 1331 916 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 690 766 2207 2417 690
Size: 76 1441 210 2457
Fragments arranged by size:
2457 1441 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2392 2702 2848 3994 2392
Size: 310 146 1146 2582
Fragments arranged by size:
2582 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1196 1196
Size: 4184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 466 546 646 785 820 2009 2441 3188
Size: 24 80 100 139 35 1189 432 747
Cuts at: 3188 3456 442
Size: 268 1170
Fragments arranged by size:
1189 1170 747 432 268 139 100 80
35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 685 685
Size: 4184
EcoO109I rG'GnC_Cy
Cuts at: 1020 1323 1020
Size: 303 3881
EcoRI G'AATT_C
Cuts at: 1078 1188 1078
Size: 110 4074
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 213 447 2203 171
Size: 42 234 1756 2152
Fragments arranged by size:
2152 1756 234 42
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 1153 1618 1866 2888 3867 1153
Size: 465 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 465 497 523 555 1004 1036 2026 2056 2058
Size: 32 26 32 449 32 990 30 2
Cuts at: 2058 2088 465
Size: 30 2561
Fragments arranged by size:
2561 990 449 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 333 1223 333
Size: 890 3294
HindIII A'AGCT_T
Cuts at: 506 506
Size: 4184
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 863 2855 3039 863
Size: 1992 184 2008
Fragments arranged by size:
2008 1992 184
MscI TGG'CCA
Cuts at: 440 1945 440
Size: 1505 2679
MslI CAynn'nnrTG
Cuts at: 2173 2300 2173
Size: 127 4057
MspA1I CmG'CkG
Cuts at: 1295 1609 1969 2982 3227 1295
Size: 314 360 1013 245 2252
Fragments arranged by size:
2252 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NcoI C'CATG_G
Cuts at: 107 107
Size: 4184
NdeI CA'TA_TG
Cuts at: 231 231
Size: 4184
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NheI G'CTAG_C
Cuts at: 120 120
Size: 4184
NspI r_CATG'y
Cuts at: 882 1062 1237 1737 2644 882
Size: 180 175 500 907 2422
Fragments arranged by size:
2422 907 500 180 175
PciI A'CATG_T
Cuts at: 878 1058 2640 878
Size: 180 1582 2422
Fragments arranged by size:
2422 1582 180
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 1020 1020
Size: 4184
PstI C_TGCA'G
Cuts at: 584 1231 1916 584
Size: 647 685 2852
Fragments arranged by size:
2852 685 647
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 1609 1969 1609
Size: 360 3824
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 1198 1198
Size: 4184
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 690 2207 2417 690
Size: 1517 210 2457
Fragments arranged by size:
2457 1517 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SexAI A'CCwGG_T
Cuts at: 935 935
Size: 4184
SfcI C'TryA_G
Cuts at: 33 580 1227 1912 2905 3096 33
Size: 547 647 685 993 191 1121
Fragments arranged by size:
1121 993 685 647 547 191
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 1339 2254 2746 3008 1339
Size: 915 492 262 2515
Fragments arranged by size:
2515 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
StyI C'CwwG_G
Cuts at: 107 1318 1672 107
Size: 1211 354 2619
Fragments arranged by size:
2619 1211 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 829 1767 1900 2359 2568 829
Size: 938 133 459 209 2445
Fragments arranged by size:
2445 938 459 209 133
TatI w'GTAC_w
Cuts at: 611 1106 3699 611
Size: 495 2593 1096
Fragments arranged by size:
2593 1096 495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4152
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 1215 1215
Size: 4184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 519 1679 519
Size: 1160 3024
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BmgBI Bpu10I BseRI BsgI
BsiWI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI