Restriction map for
pF1KB8284
(Circular) (Six-base) MAPSORT of: pF1KB8284.seq Check: 4496 from: 1 to: 4349
pF1KB8284 4349 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1280 1387 1280
Size: 107 4242
Acc65I G'GTAC_C
Cuts at: 664 1365 664
Size: 701 3648
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 553 625 1082 1327 2174 2606 3353 3621
Size: 201 72 457 245 847 432 747 268
Cuts at: 3621 352
Size: 1080
Fragments arranged by size:
1080 847 747 457 432 268 245 201
72
AflIII A'CryG_T
Cuts at: 203 550 1701 2805 3782 203
Size: 347 1151 1104 977 770
Fragments arranged by size:
1151 1104 977 770 347
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AhdI GACnn_n'nnGTC
Cuts at: 606 606
Size: 4349
AleI CACnn'nnGTG
Cuts at: 797 1147 797
Size: 350 3999
AlwNI CAG_nnn'CTG
Cuts at: 576 1105 1654 3221 576
Size: 529 549 1567 1704
Fragments arranged by size:
1704 1567 549 529
ApaI G_GGCC'C
Cuts at: 1048 1048
Size: 4349
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 557 596 795 1369 557
Size: 39 199 574 3537
Fragments arranged by size:
3537 574 199 39
BamHI G'GATC_C
Cuts at: 116 1374 116
Size: 1258 3091
BanI G'GyrC_C
Cuts at: 664 1365 1779 2027 2062 664
Size: 701 414 248 35 2951
Fragments arranged by size:
2951 701 414 248 35
BanII G_rGCy'C
Cuts at: 1048 1363 2393 1048
Size: 315 1030 3004
Fragments arranged by size:
3004 1030 315
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 602 602
Size: 4349
BciVI GTATCCnnnnn_n'
Cuts at: 1148 2247 3008 4349 1148
Size: 1099 761 1341 1148
Fragments arranged by size:
1341 1148 1099 761
BclI T'GATC_A
Cuts at: 441 1025 1869 441
Size: 584 844 2921
Fragments arranged by size:
2921 844 584
BglI GCCn_nnn'nGGC
Cuts at: 279 279
Size: 4349
BglII A'GATC_T
Cuts at: 1217 1864 3901 1217
Size: 647 2037 1665
Fragments arranged by size:
2037 1665 647
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 558 1048 1974 2067 3123 558
Size: 490 926 93 1056 1784
Fragments arranged by size:
1784 1056 926 490 93
BmgBI CAC'GTC
Cuts at: 301 301
Size: 4349
BmrI ACTGGGnnnn_n'
Cuts at: 1561 1642 1977 4121 1561
Size: 81 335 2144 1789
Fragments arranged by size:
2144 1789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 654 1144 187
Size: 467 490 3392
Fragments arranged by size:
3392 490 467
Bpu10I CC'TnA_GC
Cuts at: 537 537
Size: 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 170 253 648 4053 170
Size: 83 395 3405 466
Fragments arranged by size:
3405 466 395 83
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 305 977 1538 2059 2737 3011 3158 3569 305
Size: 672 561 521 678 274 147 411 1085
Fragments arranged by size:
1085 678 672 561 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 860 860
Size: 4349
BseYI C'CCAG_C
Cuts at: 121 326 687 721 1774 2532 3109 3605 121
Size: 205 361 34 1053 758 577 496 865
Fragments arranged by size:
1053 865 758 577 496 361 205 34
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 173 1363 2141 2331 3123 173
Size: 1190 778 190 792 1399
Fragments arranged by size:
1399 1190 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 291 291
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 173 558 1048 1363 1974 2067 2141 2331 2393
Size: 385 490 315 611 93 74 190 62
Cuts at: 2393 3123 173
Size: 730 1399
Fragments arranged by size:
1399 730 611 490 385 315 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 1202 3525 1202
Size: 2323 2026
BspMI ACCTGCnnnn'nnnn_
Cuts at: 899 1121 1399 1613 1915 2296 899
Size: 222 278 214 302 381 2952
Fragments arranged by size:
2952 381 302 278 222 214
BsrBI CCG'CTC
Cuts at: 50 114 812 2641 2695 4179 4344 50
Size: 64 698 1829 54 1484 165 55
Fragments arranged by size:
1829 1484 698 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 461 2261 461
Size: 1800 2549
BsrFI r'CCGG_y
Cuts at: 278 2347 2528 2737 278
Size: 2069 181 209 1890
Fragments arranged by size:
2069 1890 209 181
BsrGI T'GTAC_A
Cuts at: 215 215
Size: 4349
BssHII G'CGCG_C
Cuts at: 125 125
Size: 4349
BssSI C'ACGA_G
Cuts at: 586 2620 2978 586
Size: 2034 358 1957
Fragments arranged by size:
2034 1957 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 702 702
Size: 4349
BstYI r'GATC_y
Cuts at: 1 116 336 1217 1374 1841 1864 2199 2445
Size: 115 220 881 157 467 23 335 246
Cuts at: 2445 3457 3543 3555 3901 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 220
157 115 86 23 12
BtgI C'CryG_G
Cuts at: 107 900 107
Size: 793 3556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 306 2299 2480 2643 306
Size: 1993 181 163 2012
Fragments arranged by size:
2012 1993 181 163
BtsI GCAGTG_nn'
Cuts at: 395 879 1023 1592 395
Size: 484 144 569 3152
Fragments arranged by size:
3152 569 484 144
DraI TTT'AAA
Cuts at: 1325 1348 1325
Size: 23 4326
DraIII CAC_nnn'GTG
Cuts at: 639 639
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 1288 2055 2913 1288
Size: 767 858 2724
Fragments arranged by size:
2724 858 767
EaeI y'GGCC_r
Cuts at: 578 731 1934 2108 2499 2526 3871 578
Size: 153 1203 174 391 27 1345 1056
Fragments arranged by size:
1345 1203 1056 391 174 153 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 1290 2372 2582 1290
Size: 1082 210 3057
Fragments arranged by size:
3057 1082 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 352 553 625 654 1082 1144 1327 2174
Size: 165 201 72 29 428 62 183 847
Cuts at: 2174 2606 3353 3621 187
Size: 432 747 268 915
Fragments arranged by size:
915 847 747 432 428 268 201 183
165 72 62 29
EcoO109I rG'GnC_Cy
Cuts at: 600 748 1488 600
Size: 148 740 3461
Fragments arranged by size:
3461 740 148
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 882 2368 882
Size: 1486 2863
FspI TGC'GCA
Cuts at: 871 2130 871
Size: 1259 3090
HaeII r_GCGC'y
Cuts at: 1783 2031 3053 4032 1783
Size: 248 1022 979 2100
Fragments arranged by size:
2100 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 592 624 774 806 914 946 1204 1209 1236
Size: 32 150 32 108 32 258 5 27
Cuts at: 1236 1241 2191 2221 2223 2253 592
Size: 5 950 30 2 30 2688
Fragments arranged by size:
2688 950 258 150 108 32 32 32
30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 1281 1388 1281
Size: 107 4242
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 668 1369 668
Size: 701 3648
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3204 3020
Size: 184 4165
MscI TGG'CCA
Cuts at: 580 2110 580
Size: 1530 2819
MslI CAynn'nnrTG
Cuts at: 797 1147 2338 2465 797
Size: 350 1191 127 2681
Fragments arranged by size:
2681 1191 350 127
MspA1I CmG'CkG
Cuts at: 330 780 1460 1774 2134 3147 3392 330
Size: 450 680 314 360 1013 245 1287
Fragments arranged by size:
1287 1013 680 450 360 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 207 418 554 1402 1902 2809 207
Size: 211 136 848 500 907 1747
Fragments arranged by size:
1747 907 848 500 211 136
PciI A'CATG_T
Cuts at: 203 550 2805 203
Size: 347 2255 1747
Fragments arranged by size:
2255 1747 347
PflMI CCAn_nnn'nTGG
Cuts at: 639 639
Size: 4349
PfoI T'CCnGG_A
Cuts at: 670 670
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 600 748 600
Size: 148 4201
PspOMI G'GGCC_C
Cuts at: 1044 1044
Size: 4349
PspXI vC'TCGA_Gb
Cuts at: 795 795
Size: 4349
PstI C_TGCA'G
Cuts at: 473 659 1396 2081 473
Size: 186 737 685 2741
Fragments arranged by size:
2741 737 685 186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 330 1774 2134 330
Size: 1444 360 2545
Fragments arranged by size:
2545 1444 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SalI G'TCGA_C
Cuts at: 1279 1386 1279
Size: 107 4242
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SfcI C'TryA_G
Cuts at: 33 469 655 1392 2077 3070 3261 33
Size: 436 186 737 685 993 191 1121
Fragments arranged by size:
1121 993 737 685 436 191 186
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 559 1371 559
Size: 812 3537
SmlI C'TyrA_G
Cuts at: 795 1504 2419 2911 3173 795
Size: 709 915 492 262 1971
Fragments arranged by size:
1971 915 709 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StuI AGG'CCT
Cuts at: 1169 1169
Size: 4349
StyI C'CwwG_G
Cuts at: 107 1483 1837 107
Size: 1376 354 2619
Fragments arranged by size:
2619 1376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 1067 1932 2065 2524 2733 640
Size: 427 865 133 459 209 2256
Fragments arranged by size:
2256 865 459 427 209 133
TatI w'GTAC_w
Cuts at: 215 3864 215
Size: 3649 700
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1844 1844
Size: 4349
XhoI C'TCGA_G
Cuts at: 795 795
Size: 4349
XmaI C'CCGG_G
Cuts at: 557 1369 557
Size: 812 3537
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsaXI BsgI BsiWI
BsmI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI