(Circular) (Six-base) MAPSORT of: pF1KB8288.seq Check: 4163 from: 1 to: 4334 pF1KB8288 4334 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 1372 1372 Size: 4334 Acc65I G'GTAC_C Cuts at: 1350 1350 Size: 4334 AclI AA'CG_TT Cuts at: 4174 4174 Size: 4334 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 400 2159 2591 3338 3606 400 Size: 1759 432 747 268 1128 Fragments arranged by size: 1759 1128 747 432 268 AflIII A'CryG_T Cuts at: 1686 2790 3767 1686 Size: 1104 977 2253 Fragments arranged by size: 2253 1104 977 AgeI A'CCGG_T Cuts at: 2722 2722 Size: 4334 AhdI GACnn_n'nnGTC Cuts at: 1136 1136 Size: 4334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 310 310 Size: 4334 AlwNI CAG_nnn'CTG Cuts at: 138 150 440 727 1639 3206 138 Size: 12 290 287 912 1567 1266 Fragments arranged by size: 1567 1266 912 290 287 12 ApaLI G'TGCA_C Cuts at: 3104 3104 Size: 4334 ApoI r'AATT_y Cuts at: 202 1338 3687 3943 202 Size: 1136 2349 256 593 Fragments arranged by size: 2349 1136 593 256 AseI AT'TA_AT Cuts at: 20 551 20 Size: 531 3803 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4334 AvaI C'yCGr_G Cuts at: 1074 1354 1074 Size: 280 4054 BamHI G'GATC_C Cuts at: 387 1078 1359 387 Size: 691 281 3362 Fragments arranged by size: 3362 691 281 BanI G'GyrC_C Cuts at: 1030 1350 1764 2012 2047 1030 Size: 320 414 248 35 3317 Fragments arranged by size: 3317 414 320 248 35 BanII G_rGCy'C Cuts at: 294 926 946 1348 2378 294 Size: 632 20 402 1030 2250 Fragments arranged by size: 2250 1030 632 402 20 BbeI G_GCGC'C Cuts at: 1768 2016 1768 Size: 248 4086 BbsI GAAGACnn'nnnn_ Cuts at: 626 626 Size: 4334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1020 1054 1125 1159 1020 Size: 34 71 34 4195 Fragments arranged by size: 4195 71 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2232 2993 4334 2232 Size: 761 1341 2232 Fragments arranged by size: 2232 1341 761 BclI T'GATC_A Cuts at: 653 1854 653 Size: 1201 3133 BglI GCCn_nnn'nGGC Cuts at: 583 748 583 Size: 165 4169 BglII A'GATC_T Cuts at: 770 1849 3886 770 Size: 1079 2037 1218 Fragments arranged by size: 2037 1218 1079 BlpI GC'TnA_GC Cuts at: 283 1446 283 Size: 1163 3171 Bme1580I G_kGCm'C Cuts at: 1959 2052 3108 1959 Size: 93 1056 3185 Fragments arranged by size: 3185 1056 93 BmgBI CAC'GTC Cuts at: 687 719 687 Size: 32 4302 BmrI ACTGGGnnnn_n' Cuts at: 1268 1546 1627 1962 4106 1268 Size: 278 81 335 2144 1496 Fragments arranged by size: 2144 1496 335 278 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2701 2701 Size: 4334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 625 910 940 1134 1185 1280 625 Size: 285 30 194 51 95 3679 Fragments arranged by size: 3679 285 194 95 51 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1510 2389 2881 3179 1510 Size: 879 492 298 2665 Fragments arranged by size: 2665 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4038 4038 Size: 4334 BsaAI yAC'GTr Cuts at: 1687 1687 Size: 4334 BsaBI GATnn'nnATC Cuts at: 6 1848 3885 6 Size: 1842 2037 455 Fragments arranged by size: 2037 1842 455 BsaHI Gr'CG_yC Cuts at: 448 575 736 1765 2013 2715 4211 448 Size: 127 161 1029 248 702 1496 571 Fragments arranged by size: 1496 1029 702 571 248 161 127 BsaWI w'CCGG_w Cuts at: 453 1523 2044 2722 2996 3143 3554 453 Size: 1070 521 678 274 147 411 1233 Fragments arranged by size: 1233 1070 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 942 972 1027 1057 942 Size: 30 55 30 4219 Fragments arranged by size: 4219 55 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 202 622 765 202 Size: 420 143 3771 Fragments arranged by size: 3771 420 143 BseYI C'CCAG_C Cuts at: 1062 1089 1759 2517 3094 3590 1062 Size: 27 670 758 577 496 1806 Fragments arranged by size: 1806 758 670 577 496 27 BsiEI CG_ry'CG Cuts at: 104 1876 1922 3130 104 Size: 1772 46 1208 1308 Fragments arranged by size: 1772 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1025 1348 2126 2316 3108 1025 Size: 323 778 190 792 2251 Fragments arranged by size: 2251 792 778 323 190 BsmBI CGTCTCn'nnnn_ Cuts at: 455 765 455 Size: 310 4024 Bsp1286I G_dGCh'C Cuts at: 294 926 946 1025 1348 1959 2052 2126 2316 Size: 632 20 79 323 611 93 74 190 Cuts at: 2316 2378 3108 294 Size: 62 730 1520 Fragments arranged by size: 1520 730 632 611 323 190 93 79 74 62 20 BspEI T'CCGG_A Cuts at: 453 3554 453 Size: 3101 1233 BspHI T'CATG_A Cuts at: 3510 3510 Size: 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1384 1598 1900 2281 1384 Size: 214 302 381 3437 Fragments arranged by size: 3437 381 302 214 BsrBI CCG'CTC Cuts at: 50 2626 2680 4164 4329 50 Size: 2576 54 1484 165 55 Fragments arranged by size: 2576 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2246 2246 Size: 4334 BsrFI r'CCGG_y Cuts at: 696 2332 2513 2722 696 Size: 1636 181 209 2308 Fragments arranged by size: 2308 1636 209 181 BsrGI T'GTAC_A Cuts at: 350 350 Size: 4334 BssSI C'ACGA_G Cuts at: 501 2605 2963 501 Size: 2104 358 1872 Fragments arranged by size: 2104 1872 358 BstAPI GCAn_nnn'nTGC Cuts at: 150 1711 150 Size: 1561 2773 BstBI TT'CG_AA Cuts at: 2695 2695 Size: 4334 BstXI CCAn_nnnn'nTGG Cuts at: 699 996 699 Size: 297 4037 BstYI r'GATC_y Cuts at: 1 374 387 770 1078 1359 1826 1849 2184 Size: 373 13 383 308 281 467 23 335 Cuts at: 2184 2430 3442 3528 3540 3886 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 467 449 383 373 346 335 308 281 246 86 23 13 12 BtgI C'CryG_G Cuts at: 107 632 107 Size: 525 3809 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1032 2284 2465 2628 1032 Size: 1252 181 163 2738 Fragments arranged by size: 2738 1252 181 163 BtsI GCAGTG_nn' Cuts at: 1141 1240 1577 1141 Size: 99 337 3898 Fragments arranged by size: 3898 337 99 DraI TTT'AAA Cuts at: 1333 1333 Size: 4334 DraIII CAC_nnn'GTG Cuts at: 128 399 128 Size: 271 4063 DrdI GACnn_nn'nnGTC Cuts at: 685 2040 2898 685 Size: 1355 858 2121 Fragments arranged by size: 2121 1355 858 EaeI y'GGCC_r Cuts at: 230 485 764 1251 1919 2093 2484 2511 3856 Size: 255 279 487 668 174 391 27 1345 Cuts at: 3856 230 Size: 708 Fragments arranged by size: 1345 708 668 487 391 279 255 174 27 EagI C'GGCC_G Cuts at: 1919 1919 Size: 4334 EarI CTCTTCn'nnn_ Cuts at: 889 971 2357 2567 889 Size: 82 1386 210 2656 Fragments arranged by size: 2656 1386 210 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 729 801 2542 2852 2998 4144 729 Size: 72 1741 310 146 1146 919 Fragments arranged by size: 1741 1146 919 310 146 72 EcoICRI GAG'CTC Cuts at: 1346 1346 Size: 4334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 400 625 910 940 1134 1185 1280 2159 2591 Size: 225 285 30 194 51 95 879 432 Cuts at: 2591 3338 3606 400 Size: 747 268 1128 Fragments arranged by size: 1128 879 747 432 285 268 225 194 95 51 30 EcoO109I rG'GnC_Cy Cuts at: 1473 1473 Size: 4334 EcoRI G'AATT_C Cuts at: 202 1338 202 Size: 1136 3198 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2353 2353 Size: 4334 FspI TGC'GCA Cuts at: 2115 2115 Size: 4334 HaeII r_GCGC'y Cuts at: 1012 1768 2016 3038 4017 1012 Size: 756 248 1022 979 1329 Fragments arranged by size: 1329 1022 979 756 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 375 407 942 974 1027 1059 1122 1154 2176 Size: 32 535 32 53 32 63 32 1022 Cuts at: 2176 2206 2208 2238 375 Size: 30 2 30 2471 Fragments arranged by size: 2471 1022 535 63 53 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1373 1373 Size: 4334 KasI G'GCGC_C Cuts at: 1764 2012 1764 Size: 248 4086 KpnI G_GTAC'C Cuts at: 1354 1354 Size: 4334 MluI A'CGCG_T Cuts at: 3767 3767 Size: 4334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3005 3189 3005 Size: 184 4150 MscI TGG'CCA Cuts at: 232 1253 2095 232 Size: 1021 842 2471 Fragments arranged by size: 2471 1021 842 MslI CAynn'nnrTG Cuts at: 1261 2323 2450 1261 Size: 1062 127 3145 Fragments arranged by size: 3145 1062 127 MspA1I CmG'CkG Cuts at: 1017 1191 1445 1759 2119 3132 3377 1017 Size: 174 254 314 360 1013 245 1974 Fragments arranged by size: 1974 1013 360 314 254 245 174 NaeI GCC'GGC Cuts at: 2515 2515 Size: 4334 NarI GG'CG_CC Cuts at: 1765 2013 1765 Size: 248 4086 NcoI C'CATG_G Cuts at: 107 107 Size: 4334 NgoMIV G'CCGG_C Cuts at: 2513 2513 Size: 4334 NspI r_CATG'y Cuts at: 1260 1387 1887 2794 1260 Size: 127 500 907 2800 Fragments arranged by size: 2800 907 500 127 PciI A'CATG_T Cuts at: 2790 2790 Size: 4334 PflMI CCAn_nnn'nTGG Cuts at: 239 239 Size: 4334 PfoI T'CCnGG_A Cuts at: 368 887 368 Size: 519 3815 PmeI GTTT'AAAC Cuts at: 1333 1333 Size: 4334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 3473 Size: 32 4302 PstI C_TGCA'G Cuts at: 431 563 1381 2066 431 Size: 132 818 685 2699 Fragments arranged by size: 2699 818 685 132 PvuI CG_AT'CG Cuts at: 104 104 Size: 4334 PvuII CAG'CTG Cuts at: 1759 2119 1759 Size: 360 3974 RsrII CG'GwC_CG Cuts at: 2529 2529 Size: 4334 SacI G_AGCT'C Cuts at: 1348 1348 Size: 4334 SalI G'TCGA_C Cuts at: 1371 1371 Size: 4334 SapI GCTCTTCn'nnn_ Cuts at: 2357 2567 2357 Size: 210 4124 SbfI CC_TGCA'GG Cuts at: 1381 1381 Size: 4334 ScaI AGT'ACT Cuts at: 1301 3851 1301 Size: 2550 1784 SfcI C'TryA_G Cuts at: 33 427 559 1377 2062 3055 3246 33 Size: 394 132 818 685 993 191 1121 Fragments arranged by size: 1121 993 818 685 394 191 132 SfoI GGC'GCC Cuts at: 1766 2014 1766 Size: 248 4086 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4334 SmaI CCC'GGG Cuts at: 1356 1356 Size: 4334 SmlI C'TyrA_G Cuts at: 1489 2404 2896 3158 1489 Size: 915 492 262 2665 Fragments arranged by size: 2665 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4334 SphI G_CATG'C Cuts at: 1387 1887 1387 Size: 500 3834 StuI AGG'CCT Cuts at: 530 530 Size: 4334 StyI C'CwwG_G Cuts at: 107 1468 1822 107 Size: 1361 354 2619 Fragments arranged by size: 2619 1361 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 821 1917 2050 2509 2718 821 Size: 1096 133 459 209 2437 Fragments arranged by size: 2437 1096 459 209 133 TatI w'GTAC_w Cuts at: 350 533 1299 3849 350 Size: 183 766 2550 835 Fragments arranged by size: 2550 835 766 183 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3851 3883 3851 Size: 32 4302 Tth111I GACn'n_nGTC Cuts at: 954 2131 954 Size: 1177 3157 XbaI T'CTAG_A Cuts at: 1365 1365 Size: 4334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1829 1829 Size: 4334 XmaI C'CCGG_G Cuts at: 1354 1354 Size: 4334 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI