Restriction map for pF1KB8288
 (Circular) (Six-base) MAPSORT of: pF1KB8288.seq  Check: 4163  from: 1  to: 4334



pF1KB8288  4334 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:28  ..

AccI GT'mk_AC

Cuts at:   1372    1372 
   Size:       4334
 
Acc65I G'GTAC_C

Cuts at:   1350    1350 
   Size:       4334
 
AclI AA'CG_TT

Cuts at:   4174    4174 
   Size:       4334
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    400    2159    2591    3338    3606     400 
   Size:       1759     432     747     268    1128

  Fragments arranged by size: 

                1759    1128     747     432     268
 
AflIII A'CryG_T

Cuts at:   1686    2790    3767    1686 
   Size:       1104     977    2253

  Fragments arranged by size: 

                2253    1104     977
 
AgeI A'CCGG_T

Cuts at:   2722    2722 
   Size:       4334
 
AhdI GACnn_n'nnGTC

Cuts at:   1136    1136 
   Size:       4334
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    310     310 
   Size:       4334
 
AlwNI CAG_nnn'CTG

Cuts at:    138     150     440     727    1639    3206     138 
   Size:         12     290     287     912    1567    1266

  Fragments arranged by size: 

                1567    1266     912     290     287      12
 
ApaLI G'TGCA_C

Cuts at:   3104    3104 
   Size:       4334
 
ApoI r'AATT_y

Cuts at:    202    1338    3687    3943     202 
   Size:       1136    2349     256     593

  Fragments arranged by size: 

                2349    1136     593     256
 
AseI AT'TA_AT

Cuts at:     20     551      20 
   Size:        531    3803
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4334
 
AvaI C'yCGr_G

Cuts at:   1074    1354    1074 
   Size:        280    4054
 
BamHI G'GATC_C

Cuts at:    387    1078    1359     387 
   Size:        691     281    3362

  Fragments arranged by size: 

                3362     691     281
 
BanI G'GyrC_C

Cuts at:   1030    1350    1764    2012    2047    1030 
   Size:        320     414     248      35    3317

  Fragments arranged by size: 

                3317     414     320     248      35
 
BanII G_rGCy'C

Cuts at:    294     926     946    1348    2378     294 
   Size:        632      20     402    1030    2250

  Fragments arranged by size: 

                2250    1030     632     402      20
 
BbeI G_GCGC'C

Cuts at:   1768    2016    1768 
   Size:        248    4086
 
BbsI GAAGACnn'nnnn_

Cuts at:    626     626 
   Size:       4334
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1020    1054    1125    1159    1020 
   Size:         34      71      34    4195

  Fragments arranged by size: 

                4195      71      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2232    2993    4334    2232 
   Size:        761    1341    2232

  Fragments arranged by size: 

                2232    1341     761
 
BclI T'GATC_A

Cuts at:    653    1854     653 
   Size:       1201    3133
 
BglI GCCn_nnn'nGGC

Cuts at:    583     748     583 
   Size:        165    4169
 
BglII A'GATC_T

Cuts at:    770    1849    3886     770 
   Size:       1079    2037    1218

  Fragments arranged by size: 

                2037    1218    1079
 
BlpI GC'TnA_GC

Cuts at:    283    1446     283 
   Size:       1163    3171
 
Bme1580I G_kGCm'C

Cuts at:   1959    2052    3108    1959 
   Size:         93    1056    3185

  Fragments arranged by size: 

                3185    1056      93
 
BmgBI CAC'GTC

Cuts at:    687     719     687 
   Size:         32    4302
 
BmrI ACTGGGnnnn_n'

Cuts at:   1268    1546    1627    1962    4106    1268 
   Size:        278      81     335    2144    1496

  Fragments arranged by size: 

                2144    1496     335     278      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2701    2701 
   Size:       4334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    625     910     940    1134    1185    1280     625 
   Size:        285      30     194      51      95    3679

  Fragments arranged by size: 

                3679     285     194      95      51      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1510    2389    2881    3179    1510 
   Size:        879     492     298    2665

  Fragments arranged by size: 

                2665     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4038    4038 
   Size:       4334
 
BsaAI yAC'GTr

Cuts at:   1687    1687 
   Size:       4334
 
BsaBI GATnn'nnATC

Cuts at:      6    1848    3885       6 
   Size:       1842    2037     455

  Fragments arranged by size: 

                2037    1842     455
 
BsaHI Gr'CG_yC

Cuts at:    448     575     736    1765    2013    2715    4211     448 
   Size:        127     161    1029     248     702    1496     571

  Fragments arranged by size: 

                1496    1029     702     571     248     161     127
 
BsaWI w'CCGG_w

Cuts at:    453    1523    2044    2722    2996    3143    3554     453 
   Size:       1070     521     678     274     147     411    1233

  Fragments arranged by size: 

                1233    1070     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    942     972    1027    1057     942 
   Size:         30      55      30    4219

  Fragments arranged by size: 

                4219      55      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    202     622     765     202 
   Size:        420     143    3771

  Fragments arranged by size: 

                3771     420     143
 
BseYI C'CCAG_C

Cuts at:   1062    1089    1759    2517    3094    3590    1062 
   Size:         27     670     758     577     496    1806

  Fragments arranged by size: 

                1806     758     670     577     496      27
 
BsiEI CG_ry'CG

Cuts at:    104    1876    1922    3130     104 
   Size:       1772      46    1208    1308

  Fragments arranged by size: 

                1772    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1025    1348    2126    2316    3108    1025 
   Size:        323     778     190     792    2251

  Fragments arranged by size: 

                2251     792     778     323     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    455     765     455 
   Size:        310    4024
 
Bsp1286I G_dGCh'C

Cuts at:    294     926     946    1025    1348    1959    2052    2126    2316 
   Size:        632      20      79     323     611      93      74     190

Cuts at:   2316    2378    3108     294 
   Size:         62     730    1520

  Fragments arranged by size: 

                1520     730     632     611     323     190      93      79
                  74      62      20
 
BspEI T'CCGG_A

Cuts at:    453    3554     453 
   Size:       3101    1233
 
BspHI T'CATG_A

Cuts at:   3510    3510 
   Size:       4334
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1384    1598    1900    2281    1384 
   Size:        214     302     381    3437

  Fragments arranged by size: 

                3437     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2626    2680    4164    4329      50 
   Size:       2576      54    1484     165      55

  Fragments arranged by size: 

                2576    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2246    2246 
   Size:       4334
 
BsrFI r'CCGG_y

Cuts at:    696    2332    2513    2722     696 
   Size:       1636     181     209    2308

  Fragments arranged by size: 

                2308    1636     209     181
 
BsrGI T'GTAC_A

Cuts at:    350     350 
   Size:       4334
 
BssSI C'ACGA_G

Cuts at:    501    2605    2963     501 
   Size:       2104     358    1872

  Fragments arranged by size: 

                2104    1872     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    150    1711     150 
   Size:       1561    2773
 
BstBI TT'CG_AA

Cuts at:   2695    2695 
   Size:       4334
 
BstXI CCAn_nnnn'nTGG

Cuts at:    699     996     699 
   Size:        297    4037
 
BstYI r'GATC_y

Cuts at:      1     374     387     770    1078    1359    1826    1849    2184 
   Size:        373      13     383     308     281     467      23     335

Cuts at:   2184    2430    3442    3528    3540    3886       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     383     373     346     335     308
                 281     246      86      23      13      12
 
BtgI C'CryG_G

Cuts at:    107     632     107 
   Size:        525    3809
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1032    2284    2465    2628    1032 
   Size:       1252     181     163    2738

  Fragments arranged by size: 

                2738    1252     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1141    1240    1577    1141 
   Size:         99     337    3898

  Fragments arranged by size: 

                3898     337      99
 
DraI TTT'AAA

Cuts at:   1333    1333 
   Size:       4334
 
DraIII CAC_nnn'GTG

Cuts at:    128     399     128 
   Size:        271    4063
 
DrdI GACnn_nn'nnGTC

Cuts at:    685    2040    2898     685 
   Size:       1355     858    2121

  Fragments arranged by size: 

                2121    1355     858
 
EaeI y'GGCC_r

Cuts at:    230     485     764    1251    1919    2093    2484    2511    3856 
   Size:        255     279     487     668     174     391      27    1345

Cuts at:   3856     230 
   Size:        708

  Fragments arranged by size: 

                1345     708     668     487     391     279     255     174
                  27
 
EagI C'GGCC_G

Cuts at:   1919    1919 
   Size:       4334
 
EarI CTCTTCn'nnn_

Cuts at:    889     971    2357    2567     889 
   Size:         82    1386     210    2656

  Fragments arranged by size: 

                2656    1386     210      82
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    729     801    2542    2852    2998    4144     729 
   Size:         72    1741     310     146    1146     919

  Fragments arranged by size: 

                1741    1146     919     310     146      72
 
EcoICRI GAG'CTC

Cuts at:   1346    1346 
   Size:       4334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    400     625     910     940    1134    1185    1280    2159    2591 
   Size:        225     285      30     194      51      95     879     432

Cuts at:   2591    3338    3606     400 
   Size:        747     268    1128

  Fragments arranged by size: 

                1128     879     747     432     285     268     225     194
                  95      51      30
 
EcoO109I rG'GnC_Cy

Cuts at:   1473    1473 
   Size:       4334
 
EcoRI G'AATT_C

Cuts at:    202    1338     202 
   Size:       1136    3198
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2353    2353 
   Size:       4334
 
FspI TGC'GCA

Cuts at:   2115    2115 
   Size:       4334
 
HaeII r_GCGC'y

Cuts at:   1012    1768    2016    3038    4017    1012 
   Size:        756     248    1022     979    1329

  Fragments arranged by size: 

                1329    1022     979     756     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    375     407     942     974    1027    1059    1122    1154    2176 
   Size:         32     535      32      53      32      63      32    1022

Cuts at:   2176    2206    2208    2238     375 
   Size:         30       2      30    2471

  Fragments arranged by size: 

                2471    1022     535      63      53      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1373    1373 
   Size:       4334
 
KasI G'GCGC_C

Cuts at:   1764    2012    1764 
   Size:        248    4086
 
KpnI G_GTAC'C

Cuts at:   1354    1354 
   Size:       4334
 
MluI A'CGCG_T

Cuts at:   3767    3767 
   Size:       4334
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3005    3189    3005 
   Size:        184    4150
 
MscI TGG'CCA

Cuts at:    232    1253    2095     232 
   Size:       1021     842    2471

  Fragments arranged by size: 

                2471    1021     842
 
MslI CAynn'nnrTG

Cuts at:   1261    2323    2450    1261 
   Size:       1062     127    3145

  Fragments arranged by size: 

                3145    1062     127
 
MspA1I CmG'CkG

Cuts at:   1017    1191    1445    1759    2119    3132    3377    1017 
   Size:        174     254     314     360    1013     245    1974

  Fragments arranged by size: 

                1974    1013     360     314     254     245     174
 
NaeI GCC'GGC

Cuts at:   2515    2515 
   Size:       4334
 
NarI GG'CG_CC

Cuts at:   1765    2013    1765 
   Size:        248    4086
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4334
 
NgoMIV G'CCGG_C

Cuts at:   2513    2513 
   Size:       4334
 
NspI r_CATG'y

Cuts at:   1260    1387    1887    2794    1260 
   Size:        127     500     907    2800

  Fragments arranged by size: 

                2800     907     500     127
 
PciI A'CATG_T

Cuts at:   2790    2790 
   Size:       4334
 
PflMI CCAn_nnn'nTGG

Cuts at:    239     239 
   Size:       4334
 
PfoI T'CCnGG_A

Cuts at:    368     887     368 
   Size:        519    3815
 
PmeI GTTT'AAAC

Cuts at:   1333    1333 
   Size:       4334
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3473    3505    3473 
   Size:         32    4302
 
PstI C_TGCA'G

Cuts at:    431     563    1381    2066     431 
   Size:        132     818     685    2699

  Fragments arranged by size: 

                2699     818     685     132
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4334
 
PvuII CAG'CTG

Cuts at:   1759    2119    1759 
   Size:        360    3974
 
RsrII CG'GwC_CG

Cuts at:   2529    2529 
   Size:       4334
 
SacI G_AGCT'C

Cuts at:   1348    1348 
   Size:       4334
 
SalI G'TCGA_C

Cuts at:   1371    1371 
   Size:       4334
 
SapI GCTCTTCn'nnn_

Cuts at:   2357    2567    2357 
   Size:        210    4124
 
SbfI CC_TGCA'GG

Cuts at:   1381    1381 
   Size:       4334
 
ScaI AGT'ACT

Cuts at:   1301    3851    1301 
   Size:       2550    1784
 
SfcI C'TryA_G

Cuts at:     33     427     559    1377    2062    3055    3246      33 
   Size:        394     132     818     685     993     191    1121

  Fragments arranged by size: 

                1121     993     818     685     394     191     132
 
SfoI GGC'GCC

Cuts at:   1766    2014    1766 
   Size:        248    4086
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4334
 
SmaI CCC'GGG

Cuts at:   1356    1356 
   Size:       4334
 
SmlI C'TyrA_G

Cuts at:   1489    2404    2896    3158    1489 
   Size:        915     492     262    2665

  Fragments arranged by size: 

                2665     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4334
 
SphI G_CATG'C

Cuts at:   1387    1887    1387 
   Size:        500    3834
 
StuI AGG'CCT

Cuts at:    530     530 
   Size:       4334
 
StyI C'CwwG_G

Cuts at:    107    1468    1822     107 
   Size:       1361     354    2619

  Fragments arranged by size: 

                2619    1361     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    821    1917    2050    2509    2718     821 
   Size:       1096     133     459     209    2437

  Fragments arranged by size: 

                2437    1096     459     209     133
 
TatI w'GTAC_w

Cuts at:    350     533    1299    3849     350 
   Size:        183     766    2550     835

  Fragments arranged by size: 

                2550     835     766     183
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3851    3883    3851 
   Size:         32    4302
 
Tth111I GACn'n_nGTC

Cuts at:    954    2131     954 
   Size:       1177    3157
 
XbaI T'CTAG_A

Cuts at:   1365    1365 
   Size:       4334
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1829    1829 
   Size:       4334
 
XmaI C'CCGG_G

Cuts at:   1354    1354 
   Size:       4334
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI    BfrBI     BmtI   Bpu10I     BsgI    BsiWI
     BsmI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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