Restriction map for
pF1KB8290
(Circular) (Six-base) MAPSORT of: pF1KB8290.seq Check: 716 from: 1 to: 4796
pF1KB8290 4796 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1834 1834
Size: 4796
Acc65I G'GTAC_C
Cuts at: 1669 1812 1669
Size: 143 4653
AclI AA'CG_TT
Cuts at: 1790 4636 1790
Size: 2846 1950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1324 1508 2621 3053 3800 4068 868
Size: 456 184 1113 432 747 268 1596
Fragments arranged by size:
1596 1113 747 456 432 268 184
AflIII A'CryG_T
Cuts at: 174 2148 3252 4229 174
Size: 1974 1104 977 741
Fragments arranged by size:
1974 1104 977 741
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1173 1205 1173
Size: 32 4764
AlwNI CAG_nnn'CTG
Cuts at: 1000 2101 3668 1000
Size: 1101 1567 2128
Fragments arranged by size:
2128 1567 1101
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 142 1800 4149 4405 142
Size: 1658 2349 256 533
Fragments arranged by size:
2349 1658 533 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 870 1816 870
Size: 946 3850
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1611 1644 1611
Size: 33 4763
BamHI G'GATC_C
Cuts at: 1821 1821
Size: 4796
BanI G'GyrC_C
Cuts at: 1410 1669 1812 2226 2474 2509 1410
Size: 259 143 414 248 35 3697
Fragments arranged by size:
3697 414 259 248 143 35
BanII G_rGCy'C
Cuts at: 1810 2840 1810
Size: 1030 3766
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 750 890 1031 750
Size: 140 141 4515
Fragments arranged by size:
4515 141 140
BbvCI CC'TCA_GC
Cuts at: 1576 1576
Size: 4796
BciVI GTATCCnnnnn_n'
Cuts at: 795 1290 1632 2694 3455 4796 795
Size: 495 342 1062 761 1341 795
Fragments arranged by size:
1341 1062 795 761 495 342
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 1908 1908
Size: 4796
Bme1580I G_kGCm'C
Cuts at: 2421 2514 3570 2421
Size: 93 1056 3647
Fragments arranged by size:
3647 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1421 1619 2008 2089 2424 4568 1421
Size: 198 389 81 335 2144 1649
Fragments arranged by size:
2144 1649 389 335 198 81
BmtI G_CTAG'C
Cuts at: 1682 1682
Size: 4796
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 574 3163 574
Size: 2589 2207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 773 833 1172 1217 704
Size: 69 60 339 45 4283
Fragments arranged by size:
4283 339 69 60 45
Bpu10I CC'TnA_GC
Cuts at: 1450 1576 1450
Size: 126 4670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 1499 1972 2851 3343 3641 751
Size: 748 473 879 492 298 1906
Fragments arranged by size:
1906 879 748 492 473 298
BsaI GGTCTCn'nnnn_
Cuts at: 896 1541 4500 896
Size: 645 2959 1192
Fragments arranged by size:
2959 1192 645
BsaAI yAC'GTr
Cuts at: 657 2149 657
Size: 1492 3304
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 492 2227 2475 3177 4673 492
Size: 1735 248 702 1496 615
Fragments arranged by size:
1735 1496 702 615 248
BsaWI w'CCGG_w
Cuts at: 1985 2506 3184 3458 3605 4016 1985
Size: 521 678 274 147 411 2765
Fragments arranged by size:
2765 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1167 1173 1197 1203 1167
Size: 6 24 6 4760
Fragments arranged by size:
4760 24 6 6
BseYI C'CCAG_C
Cuts at: 585 1127 2221 2979 3556 4052 585
Size: 542 1094 758 577 496 1329
Fragments arranged by size:
1329 1094 758 577 542 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1451 679
Size: 772 4024
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 389 1120 1810 2588 2778 3570 389
Size: 731 690 778 190 792 1615
Fragments arranged by size:
1615 792 778 731 690 190
Bsp1286I G_dGCh'C
Cuts at: 389 1120 1810 2421 2514 2588 2778 2840 3570
Size: 731 690 611 93 74 190 62 730
Cuts at: 3570 389
Size: 1615
Fragments arranged by size:
1615 731 730 690 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 560 858 3972 560
Size: 298 3114 1384
Fragments arranged by size:
3114 1384 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 1846 2060 2362 2743 215
Size: 1631 214 302 381 2268
Fragments arranged by size:
2268 1631 381 302 214
BsrBI CCG'CTC
Cuts at: 50 555 3088 3142 4626 4791 50
Size: 505 2533 54 1484 165 55
Fragments arranged by size:
2533 1484 505 165 55 54
BsrDI GCAATG_nn'
Cuts at: 573 2708 573
Size: 2135 2661
BsrFI r'CCGG_y
Cuts at: 1722 2794 2975 3184 1722
Size: 1072 181 209 3334
Fragments arranged by size:
3334 1072 209 181
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstEII G'GTnAC_C
Cuts at: 724 724
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 811 811
Size: 4796
BstYI r'GATC_y
Cuts at: 1 498 1821 2288 2311 2646 2892 3904 3990
Size: 497 1323 467 23 335 246 1012 86
Cuts at: 3990 4002 4348 1
Size: 12 346 449
Fragments arranged by size:
1323 1012 497 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1292 1503 1292
Size: 211 4585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 1368 1439 2039 1368
Size: 71 600 4125
Fragments arranged by size:
4125 600 71
ClaI AT'CG_AT
Cuts at: 1524 1524
Size: 4796
DraI TTT'AAA
Cuts at: 234 1795 234
Size: 1561 3235
DraIII CAC_nnn'GTG
Cuts at: 595 1165 595
Size: 570 4226
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 1389 1724 2381 2555 2946 2973 4318 1389
Size: 335 657 174 391 27 1345 1867
Fragments arranged by size:
1867 1345 657 391 335 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3004 3314 3460 4606 3004
Size: 310 146 1146 3194
Fragments arranged by size:
3194 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 773 833 868 1172 1217 1324 1508 2621
Size: 69 60 35 304 45 107 184 1113
Cuts at: 2621 3053 3800 4068 704
Size: 432 747 268 1432
Fragments arranged by size:
1432 1113 747 432 304 268 184 107
69 60 45 35
EcoNI CCTnn'n_nnAGG
Cuts at: 472 472
Size: 4796
EcoO109I rG'GnC_Cy
Cuts at: 1935 1935
Size: 4796
EcoRI G'AATT_C
Cuts at: 1800 1800
Size: 4796
EcoRV GAT'ATC
Cuts at: 1273 1273
Size: 4796
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 304 1243 2815 304
Size: 939 1572 2285
Fragments arranged by size:
2285 1572 939
FspI TGC'GCA
Cuts at: 349 2577 349
Size: 2228 2568
HaeII r_GCGC'y
Cuts at: 2230 2478 3500 4479 2230
Size: 248 1022 979 2547
Fragments arranged by size:
2547 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 853 885 1584 1616 2638 2668 2670
Size: 32 719 32 699 32 1022 30 2
Cuts at: 2670 2700 102
Size: 30 2198
Fragments arranged by size:
2198 1022 719 699 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 819 1835 819
Size: 1016 3780
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1673 1816 1673
Size: 143 4653
MfeI C'AATT_G
Cuts at: 185 827 185
Size: 642 4154
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1237 1285 3467 3651 1069
Size: 168 48 2182 184 2214
Fragments arranged by size:
2214 2182 184 168 48
MscI TGG'CCA
Cuts at: 1391 2557 1391
Size: 1166 3630
MslI CAynn'nnrTG
Cuts at: 179 1427 2785 2912 179
Size: 1248 1358 127 2063
Fragments arranged by size:
2063 1358 1248 127
MspA1I CmG'CkG
Cuts at: 997 1575 1907 2221 2581 3594 3839 997
Size: 578 332 314 360 1013 245 1954
Fragments arranged by size:
1954 1013 578 360 332 314 245
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NheI G'CTAG_C
Cuts at: 1678 1678
Size: 4796
NspI r_CATG'y
Cuts at: 178 1849 2349 3256 178
Size: 1671 500 907 1718
Fragments arranged by size:
1718 1671 907 500
PasI CC'CwG_GG
Cuts at: 804 804
Size: 4796
PciI A'CATG_T
Cuts at: 174 3252 174
Size: 3078 1718
PflMI CCAn_nnn'nTGG
Cuts at: 1000 1000
Size: 4796
PfoI T'CCnGG_A
Cuts at: 1140 1140
Size: 4796
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1173 1205 3935 3967 1173
Size: 32 2730 32 2002
Fragments arranged by size:
2730 2002 32 32
PstI C_TGCA'G
Cuts at: 212 1843 2528 212
Size: 1631 685 2480
Fragments arranged by size:
2480 1631 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 997 1575 2221 2581 997
Size: 578 646 360 3212
Fragments arranged by size:
3212 646 578 360
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 212 1843 212
Size: 1631 3165
ScaI AGT'ACT
Cuts at: 229 4313 229
Size: 4084 712
SfcI C'TryA_G
Cuts at: 33 208 962 1839 2524 3517 3708 33
Size: 175 754 877 685 993 191 1121
Fragments arranged by size:
1121 993 877 754 685 191 175
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 730 1514 1951 2866 3358 3620 730
Size: 784 437 915 492 262 1906
Fragments arranged by size:
1906 915 784 492 437 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
StyI C'CwwG_G
Cuts at: 533 1930 2284 533
Size: 1397 354 3045
Fragments arranged by size:
3045 1397 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 1728 2379 2512 2971 3180 164
Size: 1564 651 133 459 209 1780
Fragments arranged by size:
1780 1564 651 459 209 133
TatI w'GTAC_w
Cuts at: 227 4311 227
Size: 4084 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 581 613 1208 1240 4313 4345 581
Size: 32 595 32 3073 32 1032
Fragments arranged by size:
3073 1032 595 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 484 2593 484
Size: 2109 2687
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1527 2291 1527
Size: 764 4032
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BcgI BfrBI BglI BmgBI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I BtgI CspCI FseI
FspAI HindIII HpaI NcoI NdeI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI