(Circular) (Six-base) MAPSORT of: pF1KB8291.seq Check: 6469 from: 1 to: 4529 pF1KB8291 4529 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 548 607 548 Size: 59 4470 AccI GT'mk_AC Cuts at: 329 1463 1567 329 Size: 1134 104 3291 Fragments arranged by size: 3291 1134 104 Acc65I G'GTAC_C Cuts at: 1545 1545 Size: 4529 AclI AA'CG_TT Cuts at: 4369 4369 Size: 4529 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 462 2354 2786 3533 3801 462 Size: 1892 432 747 268 1190 Fragments arranged by size: 1892 1190 747 432 268 AfeI AGC'GCT Cuts at: 773 773 Size: 4529 AflIII A'CryG_T Cuts at: 1881 2985 3962 1881 Size: 1104 977 2448 Fragments arranged by size: 2448 1104 977 AgeI A'CCGG_T Cuts at: 843 2917 843 Size: 2074 2455 AleI CACnn'nnGTG Cuts at: 271 271 Size: 4529 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 289 1292 289 Size: 1003 3526 AlwNI CAG_nnn'CTG Cuts at: 259 528 1180 1329 1834 3401 259 Size: 269 652 149 505 1567 1387 Fragments arranged by size: 1567 1387 652 505 269 149 ApaI G_GGCC'C Cuts at: 656 656 Size: 4529 ApaLI G'TGCA_C Cuts at: 3299 3299 Size: 4529 ApoI r'AATT_y Cuts at: 708 1533 3882 4138 708 Size: 825 2349 256 1099 Fragments arranged by size: 2349 1099 825 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4529 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4529 AvaI C'yCGr_G Cuts at: 1549 1549 Size: 4529 BamHI G'GATC_C Cuts at: 1554 1554 Size: 4529 BanI G'GyrC_C Cuts at: 335 1276 1545 1959 2207 2242 335 Size: 941 269 414 248 35 2622 Fragments arranged by size: 2622 941 414 269 248 35 BanII G_rGCy'C Cuts at: 397 656 1543 2573 397 Size: 259 887 1030 2353 Fragments arranged by size: 2353 1030 887 259 BbeI G_GCGC'C Cuts at: 1963 2211 1963 Size: 248 4281 BbvCI CC'TCA_GC Cuts at: 252 768 252 Size: 516 4013 BciVI GTATCCnnnnn_n' Cuts at: 2427 3188 4529 2427 Size: 761 1341 2427 Fragments arranged by size: 2427 1341 761 BclI T'GATC_A Cuts at: 2049 2049 Size: 4529 BglI GCCn_nnn'nGGC Cuts at: 1351 1351 Size: 4529 BglII A'GATC_T Cuts at: 2044 4081 2044 Size: 2037 2492 BlpI GC'TnA_GC Cuts at: 1641 1641 Size: 4529 Bme1580I G_kGCm'C Cuts at: 278 285 656 1281 2154 2247 3303 278 Size: 7 371 625 873 93 1056 1504 Fragments arranged by size: 1504 1056 873 625 371 93 7 BmrI ACTGGGnnnn_n' Cuts at: 272 1741 1822 2157 4301 272 Size: 1469 81 335 2144 500 Fragments arranged by size: 2144 1469 500 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2896 2896 Size: 4529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 328 565 571 760 1471 1540 328 Size: 237 6 189 711 69 3317 Fragments arranged by size: 3317 711 237 189 69 6 Bpu10I CC'TnA_GC Cuts at: 252 634 768 252 Size: 382 134 4013 Fragments arranged by size: 4013 382 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1705 2584 3076 3374 1705 Size: 879 492 298 2860 Fragments arranged by size: 2860 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4233 4233 Size: 4529 BsaAI yAC'GTr Cuts at: 1882 1882 Size: 4529 BsaBI GATnn'nnATC Cuts at: 6 2043 4080 6 Size: 2037 2037 455 Fragments arranged by size: 2037 2037 455 BsaHI Gr'CG_yC Cuts at: 1960 2208 2910 4406 1960 Size: 248 702 1496 2083 Fragments arranged by size: 2083 1496 702 248 BsaWI w'CCGG_w Cuts at: 727 843 1718 2239 2917 3191 3338 3749 727 Size: 116 875 521 678 274 147 411 1507 Fragments arranged by size: 1507 875 678 521 411 274 147 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 658 683 688 713 925 955 1498 1528 658 Size: 25 5 25 212 30 543 30 3659 Fragments arranged by size: 3659 543 212 30 30 25 25 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 998 1447 1450 1453 1456 1459 1468 1471 998 Size: 449 3 3 3 3 9 3 4056 Fragments arranged by size: 4056 449 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 126 209 302 762 1954 2712 3289 3785 126 Size: 83 93 460 1192 758 577 496 870 Fragments arranged by size: 1192 870 758 577 496 460 93 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1361 1361 Size: 4529 BsiEI CG_ry'CG Cuts at: 104 2071 2117 3325 104 Size: 1967 46 1208 1308 Fragments arranged by size: 1967 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 467 1543 2321 2511 3303 467 Size: 1076 778 190 792 1693 Fragments arranged by size: 1693 1076 792 778 190 Bsp1286I G_dGCh'C Cuts at: 278 285 397 467 656 1281 1543 2154 2247 Size: 7 112 70 189 625 262 611 93 Cuts at: 2247 2321 2511 2573 3303 278 Size: 74 190 62 730 1504 Fragments arranged by size: 1504 730 625 611 262 190 189 112 93 74 70 62 7 BspEI T'CCGG_A Cuts at: 727 3749 727 Size: 3022 1507 BspHI T'CATG_A Cuts at: 3705 3705 Size: 4529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 548 607 638 1328 1579 1793 2095 2476 548 Size: 59 31 690 251 214 302 381 2601 Fragments arranged by size: 2601 690 381 302 251 214 59 31 BsrBI CCG'CTC Cuts at: 50 136 351 2821 2875 4359 4524 50 Size: 86 215 2470 54 1484 165 55 Fragments arranged by size: 2470 1484 215 165 86 55 54 BsrDI GCAATG_nn' Cuts at: 2441 2441 Size: 4529 BsrFI r'CCGG_y Cuts at: 843 1214 2527 2708 2917 843 Size: 371 1313 181 209 2455 Fragments arranged by size: 2455 1313 371 209 181 BssSI C'ACGA_G Cuts at: 2800 3158 2800 Size: 358 4171 BstAPI GCAn_nnn'nTGC Cuts at: 1041 1155 1906 1041 Size: 114 751 3664 Fragments arranged by size: 3664 751 114 BstBI TT'CG_AA Cuts at: 2890 2890 Size: 4529 BstEII G'GTnAC_C Cuts at: 846 855 876 846 Size: 9 21 4499 Fragments arranged by size: 4499 21 9 BstXI CCAn_nnnn'nTGG Cuts at: 1311 1311 Size: 4529 BstYI r'GATC_y Cuts at: 1 908 1176 1554 2021 2044 2379 2625 3637 Size: 907 268 378 467 23 335 246 1012 Cuts at: 3637 3723 3735 4081 1 Size: 86 12 346 449 Fragments arranged by size: 1012 907 467 449 378 346 335 268 246 86 23 12 BstZ17I GTA'TAC Cuts at: 330 330 Size: 4529 BtgI C'CryG_G Cuts at: 851 1055 1349 851 Size: 204 294 4031 Fragments arranged by size: 4031 294 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 497 774 1230 2479 2660 2823 497 Size: 277 456 1249 181 163 2203 Fragments arranged by size: 2203 1249 456 277 181 163 BtsI GCAGTG_nn' Cuts at: 1772 1772 Size: 4529 DraI TTT'AAA Cuts at: 1528 1528 Size: 4529 DrdI GACnn_nn'nnGTC Cuts at: 2235 3093 2235 Size: 858 3671 EaeI y'GGCC_r Cuts at: 149 1352 2114 2288 2679 2706 4051 149 Size: 1203 762 174 391 27 1345 627 Fragments arranged by size: 1345 1203 762 627 391 174 27 EagI C'GGCC_G Cuts at: 2114 2114 Size: 4529 EarI CTCTTCn'nnn_ Cuts at: 1033 1063 1403 1424 2552 2762 1033 Size: 30 340 21 1128 210 2800 Fragments arranged by size: 2800 1128 340 210 30 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 402 2737 3047 3193 4339 402 Size: 2335 310 146 1146 592 Fragments arranged by size: 2335 1146 592 310 146 EcoICRI GAG'CTC Cuts at: 1541 1541 Size: 4529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 328 462 565 571 760 1471 1540 2354 2786 Size: 134 103 6 189 711 69 814 432 Cuts at: 2786 3533 3801 328 Size: 747 268 1056 Fragments arranged by size: 1056 814 747 711 432 268 189 134 103 69 6 EcoO109I rG'GnC_Cy Cuts at: 525 652 653 696 1668 525 Size: 127 1 43 972 3386 Fragments arranged by size: 3386 972 127 43 1 EcoRI G'AATT_C Cuts at: 708 1533 708 Size: 825 3704 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2548 Size: 4529 FspI TGC'GCA Cuts at: 2310 2310 Size: 4529 HaeII r_GCGC'y Cuts at: 775 1963 2211 3233 4212 775 Size: 1188 248 1022 979 1092 Fragments arranged by size: 1188 1092 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 925 952 957 984 1191 1223 2371 2401 2403 Size: 27 5 27 207 32 1148 30 2 Cuts at: 2403 2433 925 Size: 30 3021 Fragments arranged by size: 3021 1148 207 32 30 30 27 27 5 2 HincII GTy'rAC Cuts at: 807 927 1464 1568 807 Size: 120 537 104 3768 Fragments arranged by size: 3768 537 120 104 HpaI GTT'AAC Cuts at: 927 927 Size: 4529 KasI G'GCGC_C Cuts at: 1959 2207 1959 Size: 248 4281 KpnI G_GTAC'C Cuts at: 1549 1549 Size: 4529 MluI A'CGCG_T Cuts at: 3962 3962 Size: 4529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3200 3384 3200 Size: 184 4345 MscI TGG'CCA Cuts at: 151 1354 2290 151 Size: 1203 936 2390 Fragments arranged by size: 2390 1203 936 MslI CAynn'nnrTG Cuts at: 271 793 1354 2518 2645 271 Size: 522 561 1164 127 2155 Fragments arranged by size: 2155 1164 561 522 127 MspA1I CmG'CkG Cuts at: 256 306 1640 1954 2314 3327 3572 256 Size: 50 1334 314 360 1013 245 1213 Fragments arranged by size: 1334 1213 1013 360 314 245 50 NaeI GCC'GGC Cuts at: 2710 2710 Size: 4529 NarI GG'CG_CC Cuts at: 1960 2208 1960 Size: 248 4281 NcoI C'CATG_G Cuts at: 851 1349 851 Size: 498 4031 NgoMIV G'CCGG_C Cuts at: 2708 2708 Size: 4529 NspI r_CATG'y Cuts at: 121 1003 1582 2082 2989 121 Size: 882 579 500 907 1661 Fragments arranged by size: 1661 907 882 579 500 PasI CC'CwG_GG Cuts at: 390 747 864 894 1282 390 Size: 357 117 30 388 3637 Fragments arranged by size: 3637 388 357 117 30 PciI A'CATG_T Cuts at: 2985 2985 Size: 4529 PflMI CCAn_nnn'nTGG Cuts at: 543 543 Size: 4529 PfoI T'CCnGG_A Cuts at: 947 947 Size: 4529 PmeI GTTT'AAAC Cuts at: 1528 1528 Size: 4529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 3668 Size: 32 4497 PpuMI rG'GwC_Cy Cuts at: 525 696 525 Size: 171 4358 PshAI GACnn'nnGTC Cuts at: 802 1483 802 Size: 681 3848 PspOMI G'GGCC_C Cuts at: 652 652 Size: 4529 PstI C_TGCA'G Cuts at: 707 1151 1265 1325 1475 1576 2261 707 Size: 444 114 60 150 101 685 2975 Fragments arranged by size: 2975 685 444 150 114 101 60 PvuI CG_AT'CG Cuts at: 104 104 Size: 4529 PvuII CAG'CTG Cuts at: 256 306 1954 2314 256 Size: 50 1648 360 2471 Fragments arranged by size: 2471 1648 360 50 RsrII CG'GwC_CG Cuts at: 2724 2724 Size: 4529 SacI G_AGCT'C Cuts at: 1543 1543 Size: 4529 SalI G'TCGA_C Cuts at: 1462 1566 1462 Size: 104 4425 SapI GCTCTTCn'nnn_ Cuts at: 1033 2552 2762 1033 Size: 1519 210 2800 Fragments arranged by size: 2800 1519 210 SbfI CC_TGCA'GG Cuts at: 1576 1576 Size: 4529 ScaI AGT'ACT Cuts at: 4046 4046 Size: 4529 SfcI C'TryA_G Cuts at: 33 703 1147 1261 1321 1471 1572 2257 3250 Size: 670 444 114 60 150 101 685 993 Cuts at: 3250 3441 33 Size: 191 1121 Fragments arranged by size: 1121 993 685 670 444 191 150 114 101 60 SfoI GGC'GCC Cuts at: 1961 2209 1961 Size: 248 4281 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4529 SmaI CCC'GGG Cuts at: 1551 1551 Size: 4529 SmlI C'TyrA_G Cuts at: 1684 2599 3091 3353 1684 Size: 915 492 262 2860 Fragments arranged by size: 2860 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4529 SphI G_CATG'C Cuts at: 1003 1582 2082 1003 Size: 579 500 3450 Fragments arranged by size: 3450 579 500 StyI C'CwwG_G Cuts at: 221 851 1349 1663 2017 221 Size: 630 498 314 354 2733 Fragments arranged by size: 2733 630 498 354 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 403 895 1237 2112 2245 2704 2913 403 Size: 492 342 875 133 459 209 2019 Fragments arranged by size: 2019 875 492 459 342 209 133 TatI w'GTAC_w Cuts at: 4044 4044 Size: 4529 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 683 715 1498 1530 4046 4078 683 Size: 32 783 32 2516 32 1134 Fragments arranged by size: 2516 1134 783 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2326 2326 Size: 4529 XbaI T'CTAG_A Cuts at: 1560 1560 Size: 4529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 423 2024 423 Size: 1601 2928 XmaI C'CCGG_G Cuts at: 1549 1549 Size: 4529 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AflII AhdI AloI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI NruI NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI