Restriction map for
pF1KB8297
(Circular) (Six-base) MAPSORT of: pF1KB8297.seq Check: 4053 from: 1 to: 3686
pF1KB8297 3686 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 232 1511 1943 2690 2958 208
Size: 24 1279 432 747 268 936
Fragments arranged by size:
1279 936 747 432 268 24
AflIII A'CryG_T
Cuts at: 1038 2142 3119 1038
Size: 1104 977 1605
Fragments arranged by size:
1605 1104 977
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 186 991 2558 186
Size: 805 1567 1314
Fragments arranged by size:
1567 1314 805
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 690 3039 3295 690
Size: 2349 256 1081
Fragments arranged by size:
2349 1081 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 136 706 136
Size: 570 3116
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 340 505 702 1116 1364 1399 340
Size: 165 197 414 248 35 2627
Fragments arranged by size:
2627 414 248 197 165 35
BanII G_rGCy'C
Cuts at: 542 700 1730 542
Size: 158 1030 2498
Fragments arranged by size:
2498 1030 158
BbeI G_GCGC'C
Cuts at: 1120 1368 1120
Size: 248 3438
BbsI GAAGACnn'nnnn_
Cuts at: 121 121
Size: 3686
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 512 1206 512
Size: 694 2992
BglI GCCn_nnn'nGGC
Cuts at: 227 227
Size: 3686
BglII A'GATC_T
Cuts at: 566 1201 3238 566
Size: 635 2037 1014
Fragments arranged by size:
2037 1014 635
BlpI GC'TnA_GC
Cuts at: 798 798
Size: 3686
Bme1580I G_kGCm'C
Cuts at: 508 1311 1404 2460 508
Size: 803 93 1056 1734
Fragments arranged by size:
1734 1056 803 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 898 979 1314 3458 423
Size: 475 81 335 2144 651
Fragments arranged by size:
2144 651 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 262 484 493 166
Size: 96 222 9 3359
Fragments arranged by size:
3359 222 96 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1741 2233 2531 862
Size: 879 492 298 2017
Fragments arranged by size:
2017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3390 3390
Size: 3686
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 287 1117 1365 2067 3563 287
Size: 830 248 702 1496 410
Fragments arranged by size:
1496 830 702 410 248
BsaWI w'CCGG_w
Cuts at: 875 1396 2074 2348 2495 2906 875
Size: 521 678 274 147 411 1655
Fragments arranged by size:
1655 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 628 259
Size: 369 3317
BseYI C'CCAG_C
Cuts at: 223 548 1111 1869 2446 2942 223
Size: 325 563 758 577 496 967
Fragments arranged by size:
967 758 577 563 496 325
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 700 1478 1668 2460 700
Size: 778 190 792 1926
Fragments arranged by size:
1926 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 508 542 700 1311 1404 1478 1668 1730 2460
Size: 34 158 611 93 74 190 62 730
Cuts at: 2460 508
Size: 1734
Fragments arranged by size:
1734 730 611 190 158 93 74 62
34
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 515 2862 515
Size: 2347 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 950 1252 1633 736
Size: 214 302 381 2789
Fragments arranged by size:
2789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1598 1598
Size: 3686
BsrFI r'CCGG_y
Cuts at: 1684 1865 2074 1684
Size: 181 209 3296
Fragments arranged by size:
3296 209 181
BssSI C'ACGA_G
Cuts at: 1957 2315 1957
Size: 358 3328
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1063
Size: 3686
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstEII G'GTnAC_C
Cuts at: 378 378
Size: 3686
BstYI r'GATC_y
Cuts at: 1 566 711 1178 1201 1536 1782 2794 2880
Size: 565 145 467 23 335 246 1012 86
Cuts at: 2880 2892 3238 1
Size: 12 346 449
Fragments arranged by size:
1012 565 467 449 346 335 246 145
86 23 12
BtgI C'CryG_G
Cuts at: 107 387 107
Size: 280 3406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1636 1817 1980 1636
Size: 181 163 3342
Fragments arranged by size:
3342 181 163
BtsI GCAGTG_nn'
Cuts at: 199 670 929 199
Size: 471 259 2956
Fragments arranged by size:
2956 471 259
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 1271 1445 1836 1863 3208 1271
Size: 174 391 27 1345 1749
Fragments arranged by size:
1749 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 1709 1919 1709
Size: 210 3476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1894 2204 2350 3496 1894
Size: 310 146 1146 2084
Fragments arranged by size:
2084 1146 310 146
EcoICRI GAG'CTC
Cuts at: 698 698
Size: 3686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 208 232 262 484 493 1511 1943 2690
Size: 42 24 30 222 9 1018 432 747
Cuts at: 2690 2958 166
Size: 268 894
Fragments arranged by size:
1018 894 747 432 268 222 42 30
24 9
EcoO109I rG'GnC_Cy
Cuts at: 150 825 150
Size: 675 3011
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3686
FspI TGC'GCA
Cuts at: 523 1467 523
Size: 944 2742
FspAI rTGC'GCAy
Cuts at: 523 523
Size: 3686
HaeII r_GCGC'y
Cuts at: 1120 1368 2390 3369 1120
Size: 248 1022 979 1437
Fragments arranged by size:
1437 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1528 1558 1560 1590 1528
Size: 30 2 30 3624
Fragments arranged by size:
3624 30 30 2
HincII GTy'rAC
Cuts at: 725 725
Size: 3686
KasI G'GCGC_C
Cuts at: 1116 1364 1116
Size: 248 3438
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 1447 1447
Size: 3686
MslI CAynn'nnrTG
Cuts at: 1675 1802 1675
Size: 127 3559
MspA1I CmG'CkG
Cuts at: 227 389 797 1111 1471 2484 2729 227
Size: 162 408 314 360 1013 245 1184
Fragments arranged by size:
1184 1013 408 360 314 245 162
NaeI GCC'GGC
Cuts at: 1867 1867
Size: 3686
NarI GG'CG_CC
Cuts at: 1117 1365 1117
Size: 248 3438
NcoI C'CATG_G
Cuts at: 107 107
Size: 3686
NgoMIV G'CCGG_C
Cuts at: 1865 1865
Size: 3686
NspI r_CATG'y
Cuts at: 675 739 1239 2146 675
Size: 64 500 907 2215
Fragments arranged by size:
2215 907 500 64
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PfoI T'CCnGG_A
Cuts at: 353 353
Size: 3686
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PpuMI rG'GwC_Cy
Cuts at: 150 150
Size: 3686
PstI C_TGCA'G
Cuts at: 665 733 1418 665
Size: 68 685 2933
Fragments arranged by size:
2933 685 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 1111 1471 1111
Size: 360 3326
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 700 700
Size: 3686
SacII CC_GC'GG
Cuts at: 390 390
Size: 3686
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 1709 1919 1709
Size: 210 3476
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 661 729 1414 2407 2598 33
Size: 628 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 628 191 68
SfoI GGC'GCC
Cuts at: 1118 1366 1118
Size: 248 3438
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 841 1756 2248 2510 841
Size: 915 492 262 2017
Fragments arranged by size:
2017 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
StyI C'CwwG_G
Cuts at: 107 820 1174 107
Size: 713 354 2619
Fragments arranged by size:
2619 713 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 1269 1402 1861 2070 366
Size: 903 133 459 209 1982
Fragments arranged by size:
1982 903 459 209 133
TatI w'GTAC_w
Cuts at: 3201 3201
Size: 3686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 3654
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 126 1181 126
Size: 1055 2631
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI