Restriction map for
pF1KB8303
(Circular) (Six-base) MAPSORT of: pF1KB8303.seq Check: 614 from: 1 to: 4913
pF1KB8303 4913 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1224 1245 1651 1664 1224
Size: 21 406 13 4473
Fragments arranged by size:
4473 406 21 13
AccI GT'mk_AC
Cuts at: 443 629 1697 1951 443
Size: 186 1068 254 3405
Fragments arranged by size:
3405 1068 254 186
Acc65I G'GTAC_C
Cuts at: 1929 1929
Size: 4913
AclI AA'CG_TT
Cuts at: 4753 4753
Size: 4913
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 793 910 1396 1510 1741 1900 2738 3170
Size: 463 117 486 114 231 159 838 432
Cuts at: 3170 3917 4185 330
Size: 747 268 1058
Fragments arranged by size:
1058 838 747 486 463 432 268 231
159 117 114
AfeI AGC'GCT
Cuts at: 751 751
Size: 4913
AflIII A'CryG_T
Cuts at: 1001 1265 2265 3369 4346 1001
Size: 264 1000 1104 977 1568
Fragments arranged by size:
1568 1104 1000 977 264
AgeI A'CCGG_T
Cuts at: 3301 3301
Size: 4913
AleI CACnn'nnGTG
Cuts at: 1816 1816
Size: 4913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1834 1834
Size: 4913
AlwNI CAG_nnn'CTG
Cuts at: 622 1107 1239 1512 2218 3785 622
Size: 485 132 273 706 1567 1750
Fragments arranged by size:
1750 1567 706 485 273 132
ApaLI G'TGCA_C
Cuts at: 1651 3683 1651
Size: 2032 2881
ApoI r'AATT_y
Cuts at: 1917 4266 4522 1917
Size: 2349 256 2308
Fragments arranged by size:
2349 2308 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4913
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4913
AvaI C'yCGr_G
Cuts at: 1216 1933 1216
Size: 717 4196
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 808 841 808
Size: 33 4880
BamHI G'GATC_C
Cuts at: 1938 1938
Size: 4913
BanI G'GyrC_C
Cuts at: 894 1177 1549 1810 1929 2343 2591 2626 894
Size: 283 372 261 119 414 248 35 3181
Fragments arranged by size:
3181 414 372 283 261 248 119 35
BanII G_rGCy'C
Cuts at: 616 851 1400 1927 2957 616
Size: 235 549 527 1030 2572
Fragments arranged by size:
2572 1030 549 527 235
BbeI G_GCGC'C
Cuts at: 2347 2595 2347
Size: 248 4665
BbvCI CC'TCA_GC
Cuts at: 1198 1198
Size: 4913
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4879
BciVI GTATCCnnnnn_n'
Cuts at: 2811 3572 4913 2811
Size: 761 1341 2811
Fragments arranged by size:
2811 1341 761
BclI T'GATC_A
Cuts at: 299 482 1556 2433 299
Size: 183 1074 877 2779
Fragments arranged by size:
2779 1074 877 183
BglII A'GATC_T
Cuts at: 1112 2428 4465 1112
Size: 1316 2037 1560
Fragments arranged by size:
2037 1560 1316
BlpI GC'TnA_GC
Cuts at: 1622 2025 1622
Size: 403 4510
Bme1580I G_kGCm'C
Cuts at: 336 899 1180 1655 2538 2631 3687 336
Size: 563 281 475 883 93 1056 1562
Fragments arranged by size:
1562 1056 883 563 475 281 93
BmgBI CAC'GTC
Cuts at: 150 150
Size: 4913
BmrI ACTGGGnnnn_n'
Cuts at: 2125 2206 2541 4685 2125
Size: 81 335 2144 2353
Fragments arranged by size:
2353 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3280 3280
Size: 4913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 571 883 1261 1765 439
Size: 132 312 378 504 3587
Fragments arranged by size:
3587 504 378 312 132
Bpu10I CC'TnA_GC
Cuts at: 1198 1198
Size: 4913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2089 2968 3460 3758 1693
Size: 396 879 492 298 2848
Fragments arranged by size:
2848 879 492 396 298
BsaI GGTCTCn'nnnn_
Cuts at: 177 781 859 1833 4617 177
Size: 604 78 974 2784 473
Fragments arranged by size:
2784 974 604 473 78
BsaAI yAC'GTr
Cuts at: 579 722 2266 579
Size: 143 1544 3226
Fragments arranged by size:
3226 1544 143
BsaBI GATnn'nnATC
Cuts at: 6 2427 4464 6
Size: 2421 2037 455
Fragments arranged by size:
2421 2037 455
BsaHI Gr'CG_yC
Cuts at: 1289 2344 2592 3294 4790 1289
Size: 1055 248 702 1496 1412
Fragments arranged by size:
1496 1412 1055 702 248
BsaWI w'CCGG_w
Cuts at: 1118 2102 2623 3301 3575 3722 4133 1118
Size: 984 521 678 274 147 411 1898
Fragments arranged by size:
1898 984 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1252 1282 1252
Size: 30 4883
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1768 1864 1768
Size: 96 4817
BseYI C'CCAG_C
Cuts at: 582 831 1096 1456 1479 1611 1854 1903 2338
Size: 249 265 360 23 132 243 49 435
Cuts at: 2338 3096 3673 4169 582
Size: 758 577 496 1326
Fragments arranged by size:
1326 758 577 496 435 360 265 249
243 132 49 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1075 1192 1528 1756 1012
Size: 63 117 336 228 4169
Fragments arranged by size:
4169 336 228 117 63
BsiEI CG_ry'CG
Cuts at: 104 234 273 1035 2455 2501 3709 104
Size: 130 39 762 1420 46 1208 1308
Fragments arranged by size:
1420 1308 1208 762 130 46 39
BsiHKAI G_wGCw'C
Cuts at: 851 1400 1628 1655 1927 2705 2895 3687 851
Size: 549 228 27 272 778 190 792 2077
Fragments arranged by size:
2077 792 778 549 272 228 190 27
BsiWI C'GTAC_G
Cuts at: 722 722
Size: 4913
BsmI GAATG_Cn'
Cuts at: 215 1091 215
Size: 876 4037
BsmBI CGTCTCn'nnnn_
Cuts at: 1426 1675 1426
Size: 249 4664
Bsp1286I G_dGCh'C
Cuts at: 336 616 851 899 1180 1400 1628 1655 1927
Size: 280 235 48 281 220 228 27 272
Cuts at: 1927 2538 2631 2705 2895 2957 3687 336
Size: 611 93 74 190 62 730 1562
Fragments arranged by size:
1562 730 611 281 280 272 235 228
220 190 93 74 62 48 27
BspEI T'CCGG_A
Cuts at: 1118 4133 1118
Size: 3015 1898
BspHI T'CATG_A
Cuts at: 1559 4089 1559
Size: 2530 2383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 710 1224 1245 1651 1664 1963 2177 2479 2860
Size: 514 21 406 13 299 214 302 381
Cuts at: 2860 710
Size: 2763
Fragments arranged by size:
2763 514 406 381 302 299 214 21
13
BsrBI CCG'CTC
Cuts at: 50 3205 3259 4743 4908 50
Size: 3155 54 1484 165 55
Fragments arranged by size:
3155 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 176 1687 2825 176
Size: 1511 1138 2264
Fragments arranged by size:
2264 1511 1138
BsrFI r'CCGG_y
Cuts at: 139 1419 2911 3092 3301 139
Size: 1280 1492 181 209 1751
Fragments arranged by size:
1751 1492 1280 209 181
BssSI C'ACGA_G
Cuts at: 784 3184 3542 784
Size: 2400 358 2155
Fragments arranged by size:
2400 2155 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1107 1512 1632 2290 1107
Size: 405 120 658 3730
Fragments arranged by size:
3730 658 405 120
BstBI TT'CG_AA
Cuts at: 3274 3274
Size: 4913
BstXI CCAn_nnnn'nTGG
Cuts at: 417 1365 417
Size: 948 3965
BstYI r'GATC_y
Cuts at: 1 974 1112 1193 1938 2405 2428 2763 3009
Size: 973 138 81 745 467 23 335 246
Cuts at: 3009 4021 4107 4119 4465 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 973 745 467 449 346 335 246
138 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 1520 1520
Size: 4913
BtgI C'CryG_G
Cuts at: 791 911 791
Size: 120 4793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2863 3044 3207 2863
Size: 181 163 4569
Fragments arranged by size:
4569 181 163
BtsI GCAGTG_nn'
Cuts at: 324 2156 324
Size: 1832 3081
DraI TTT'AAA
Cuts at: 1912 1912
Size: 4913
DraIII CAC_nnn'GTG
Cuts at: 1734 1734
Size: 4913
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2619 3477 1767
Size: 852 858 3203
Fragments arranged by size:
3203 858 852
EaeI y'GGCC_r
Cuts at: 141 270 1185 1421 2498 2672 3063 3090 4435
Size: 129 915 236 1077 174 391 27 1345
Cuts at: 4435 141
Size: 619
Fragments arranged by size:
1345 1077 915 619 391 236 174 129
27
EagI C'GGCC_G
Cuts at: 270 2498 270
Size: 2228 2685
EarI CTCTTCn'nnn_
Cuts at: 2936 3146 2936
Size: 210 4703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3121 3431 3577 4723 3121
Size: 310 146 1146 3311
Fragments arranged by size:
3311 1146 310 146
EcoICRI GAG'CTC
Cuts at: 849 1398 1925 849
Size: 549 527 3837
Fragments arranged by size:
3837 549 527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 439 571 793 883 910 1261 1396 1510
Size: 109 132 222 90 27 351 135 114
Cuts at: 1510 1741 1765 1900 2738 3170 3917 4185 330
Size: 231 24 135 838 432 747 268 1058
Fragments arranged by size:
1058 838 747 432 351 268 231 222
135 135 132 114 109 90 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1780 1894 1780
Size: 114 4799
EcoO109I rG'GnC_Cy
Cuts at: 1803 1887 1899 2052 1803
Size: 84 12 153 4664
Fragments arranged by size:
4664 153 84 12
EcoRI G'AATT_C
Cuts at: 1917 1917
Size: 4913
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 4913
FseI GG_CCGG'CC
Cuts at: 143 143
Size: 4913
FspI TGC'GCA
Cuts at: 2694 2694
Size: 4913
HaeII r_GCGC'y
Cuts at: 753 2347 2595 3617 4596 753
Size: 1594 248 1022 979 1070
Fragments arranged by size:
1594 1070 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1252 1284 2755 2785 2787 2817 1252
Size: 32 1471 30 2 30 3348
Fragments arranged by size:
3348 1471 32 30 30 2
HincII GTy'rAC
Cuts at: 1407 1952 1407
Size: 545 4368
KasI G'GCGC_C
Cuts at: 2343 2591 2343
Size: 248 4665
KpnI G_GTAC'C
Cuts at: 1933 1933
Size: 4913
MluI A'CGCG_T
Cuts at: 4346 4346
Size: 4913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3584 3768 3584
Size: 184 4729
MscI TGG'CCA
Cuts at: 2674 2674
Size: 4913
MslI CAynn'nnrTG
Cuts at: 379 1658 1816 2902 3029 379
Size: 1279 158 1086 127 2263
Fragments arranged by size:
2263 1279 1086 158 127
MspA1I CmG'CkG
Cuts at: 1202 1209 1308 1632 2024 2338 2698 3711 3956
Size: 7 99 324 392 314 360 1013 245
Cuts at: 3956 1202
Size: 2159
Fragments arranged by size:
2159 1013 392 360 324 314 245 99
7
NaeI GCC'GGC
Cuts at: 141 1421 3094 141
Size: 1280 1673 1960
Fragments arranged by size:
1960 1673 1280
NarI GG'CG_CC
Cuts at: 2344 2592 2344
Size: 248 4665
NgoMIV G'CCGG_C
Cuts at: 139 1419 3092 139
Size: 1280 1673 1960
Fragments arranged by size:
1960 1673 1280
NspI r_CATG'y
Cuts at: 1005 1269 1419 1966 2466 3373 1005
Size: 264 150 547 500 907 2545
Fragments arranged by size:
2545 907 547 500 264 150
PciI A'CATG_T
Cuts at: 1001 1265 3369 1001
Size: 264 2104 2545
Fragments arranged by size:
2545 2104 264
PflMI CCAn_nnn'nTGG
Cuts at: 150 582 150
Size: 432 4481
PfoI T'CCnGG_A
Cuts at: 287 860 1745 1831 287
Size: 573 885 86 3369
Fragments arranged by size:
3369 885 573 86
PmeI GTTT'AAAC
Cuts at: 1912 1912
Size: 4913
PmlI CAC'GTG
Cuts at: 579 579
Size: 4913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 4052
Size: 32 4881
PpuMI rG'GwC_Cy
Cuts at: 1803 1803
Size: 4913
PsiI TTA'TAA
Cuts at: 644 644
Size: 4913
PstI C_TGCA'G
Cuts at: 1112 1960 2645 1112
Size: 848 685 3380
Fragments arranged by size:
3380 848 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4913
PvuII CAG'CTG
Cuts at: 1308 1632 2338 2698 1308
Size: 324 706 360 3523
Fragments arranged by size:
3523 706 360 324
RsrII CG'GwC_CG
Cuts at: 3108 3108
Size: 4913
SacI G_AGCT'C
Cuts at: 851 1400 1927 851
Size: 549 527 3837
Fragments arranged by size:
3837 549 527
SalI G'TCGA_C
Cuts at: 1950 1950
Size: 4913
SapI GCTCTTCn'nnn_
Cuts at: 2936 3146 2936
Size: 210 4703
SbfI CC_TGCA'GG
Cuts at: 1960 1960
Size: 4913
ScaI AGT'ACT
Cuts at: 4430 4430
Size: 4913
SfcI C'TryA_G
Cuts at: 33 1108 1698 1956 2641 3634 3825 33
Size: 1075 590 258 685 993 191 1121
Fragments arranged by size:
1121 1075 993 685 590 258 191
SfoI GGC'GCC
Cuts at: 2345 2593 2345
Size: 248 4665
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4913
SmaI CCC'GGG
Cuts at: 1935 1935
Size: 4913
SmlI C'TyrA_G
Cuts at: 1216 1708 2068 2983 3475 3737 1216
Size: 492 360 915 492 262 2392
Fragments arranged by size:
2392 915 492 492 360 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4913
SphI G_CATG'C
Cuts at: 1966 2466 1966
Size: 500 4413
StuI AGG'CCT
Cuts at: 562 1027 562
Size: 465 4448
StyI C'CwwG_G
Cuts at: 131 1292 1814 2047 2401 131
Size: 1161 522 233 354 2643
Fragments arranged by size:
2643 1161 522 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 248 277 409 674 937 1074 2496 2629 3088
Size: 29 132 265 263 137 1422 133 459
Cuts at: 3088 3297 248
Size: 209 1864
Fragments arranged by size:
1864 1422 459 265 263 209 137 133
132 29
TatI w'GTAC_w
Cuts at: 1262 4428 1262
Size: 3166 1747
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4430 4462 4430
Size: 32 4881
Tth111I GACn'n_nGTC
Cuts at: 2710 2710
Size: 4913
XbaI T'CTAG_A
Cuts at: 1944 1944
Size: 4913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1821 2408 1821
Size: 587 4326
XhoI C'TCGA_G
Cuts at: 1216 1216
Size: 4913
XmaI C'CCGG_G
Cuts at: 1933 1933
Size: 4913
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BbsI
BfrBI BglI BmtI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI