(Circular) (Six-base) MAPSORT of: pF1KB8303.seq Check: 614 from: 1 to: 4913 pF1KB8303 4913 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1224 1245 1651 1664 1224 Size: 21 406 13 4473 Fragments arranged by size: 4473 406 21 13 AccI GT'mk_AC Cuts at: 443 629 1697 1951 443 Size: 186 1068 254 3405 Fragments arranged by size: 3405 1068 254 186 Acc65I G'GTAC_C Cuts at: 1929 1929 Size: 4913 AclI AA'CG_TT Cuts at: 4753 4753 Size: 4913 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 330 793 910 1396 1510 1741 1900 2738 3170 Size: 463 117 486 114 231 159 838 432 Cuts at: 3170 3917 4185 330 Size: 747 268 1058 Fragments arranged by size: 1058 838 747 486 463 432 268 231 159 117 114 AfeI AGC'GCT Cuts at: 751 751 Size: 4913 AflIII A'CryG_T Cuts at: 1001 1265 2265 3369 4346 1001 Size: 264 1000 1104 977 1568 Fragments arranged by size: 1568 1104 1000 977 264 AgeI A'CCGG_T Cuts at: 3301 3301 Size: 4913 AleI CACnn'nnGTG Cuts at: 1816 1816 Size: 4913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1834 1834 Size: 4913 AlwNI CAG_nnn'CTG Cuts at: 622 1107 1239 1512 2218 3785 622 Size: 485 132 273 706 1567 1750 Fragments arranged by size: 1750 1567 706 485 273 132 ApaLI G'TGCA_C Cuts at: 1651 3683 1651 Size: 2032 2881 ApoI r'AATT_y Cuts at: 1917 4266 4522 1917 Size: 2349 256 2308 Fragments arranged by size: 2349 2308 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4913 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4913 AvaI C'yCGr_G Cuts at: 1216 1933 1216 Size: 717 4196 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 808 841 808 Size: 33 4880 BamHI G'GATC_C Cuts at: 1938 1938 Size: 4913 BanI G'GyrC_C Cuts at: 894 1177 1549 1810 1929 2343 2591 2626 894 Size: 283 372 261 119 414 248 35 3181 Fragments arranged by size: 3181 414 372 283 261 248 119 35 BanII G_rGCy'C Cuts at: 616 851 1400 1927 2957 616 Size: 235 549 527 1030 2572 Fragments arranged by size: 2572 1030 549 527 235 BbeI G_GCGC'C Cuts at: 2347 2595 2347 Size: 248 4665 BbvCI CC'TCA_GC Cuts at: 1198 1198 Size: 4913 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4879 BciVI GTATCCnnnnn_n' Cuts at: 2811 3572 4913 2811 Size: 761 1341 2811 Fragments arranged by size: 2811 1341 761 BclI T'GATC_A Cuts at: 299 482 1556 2433 299 Size: 183 1074 877 2779 Fragments arranged by size: 2779 1074 877 183 BglII A'GATC_T Cuts at: 1112 2428 4465 1112 Size: 1316 2037 1560 Fragments arranged by size: 2037 1560 1316 BlpI GC'TnA_GC Cuts at: 1622 2025 1622 Size: 403 4510 Bme1580I G_kGCm'C Cuts at: 336 899 1180 1655 2538 2631 3687 336 Size: 563 281 475 883 93 1056 1562 Fragments arranged by size: 1562 1056 883 563 475 281 93 BmgBI CAC'GTC Cuts at: 150 150 Size: 4913 BmrI ACTGGGnnnn_n' Cuts at: 2125 2206 2541 4685 2125 Size: 81 335 2144 2353 Fragments arranged by size: 2353 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3280 3280 Size: 4913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 439 571 883 1261 1765 439 Size: 132 312 378 504 3587 Fragments arranged by size: 3587 504 378 312 132 Bpu10I CC'TnA_GC Cuts at: 1198 1198 Size: 4913 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1693 2089 2968 3460 3758 1693 Size: 396 879 492 298 2848 Fragments arranged by size: 2848 879 492 396 298 BsaI GGTCTCn'nnnn_ Cuts at: 177 781 859 1833 4617 177 Size: 604 78 974 2784 473 Fragments arranged by size: 2784 974 604 473 78 BsaAI yAC'GTr Cuts at: 579 722 2266 579 Size: 143 1544 3226 Fragments arranged by size: 3226 1544 143 BsaBI GATnn'nnATC Cuts at: 6 2427 4464 6 Size: 2421 2037 455 Fragments arranged by size: 2421 2037 455 BsaHI Gr'CG_yC Cuts at: 1289 2344 2592 3294 4790 1289 Size: 1055 248 702 1496 1412 Fragments arranged by size: 1496 1412 1055 702 248 BsaWI w'CCGG_w Cuts at: 1118 2102 2623 3301 3575 3722 4133 1118 Size: 984 521 678 274 147 411 1898 Fragments arranged by size: 1898 984 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1252 1282 1252 Size: 30 4883 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1768 1864 1768 Size: 96 4817 BseYI C'CCAG_C Cuts at: 582 831 1096 1456 1479 1611 1854 1903 2338 Size: 249 265 360 23 132 243 49 435 Cuts at: 2338 3096 3673 4169 582 Size: 758 577 496 1326 Fragments arranged by size: 1326 758 577 496 435 360 265 249 243 132 49 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1012 1075 1192 1528 1756 1012 Size: 63 117 336 228 4169 Fragments arranged by size: 4169 336 228 117 63 BsiEI CG_ry'CG Cuts at: 104 234 273 1035 2455 2501 3709 104 Size: 130 39 762 1420 46 1208 1308 Fragments arranged by size: 1420 1308 1208 762 130 46 39 BsiHKAI G_wGCw'C Cuts at: 851 1400 1628 1655 1927 2705 2895 3687 851 Size: 549 228 27 272 778 190 792 2077 Fragments arranged by size: 2077 792 778 549 272 228 190 27 BsiWI C'GTAC_G Cuts at: 722 722 Size: 4913 BsmI GAATG_Cn' Cuts at: 215 1091 215 Size: 876 4037 BsmBI CGTCTCn'nnnn_ Cuts at: 1426 1675 1426 Size: 249 4664 Bsp1286I G_dGCh'C Cuts at: 336 616 851 899 1180 1400 1628 1655 1927 Size: 280 235 48 281 220 228 27 272 Cuts at: 1927 2538 2631 2705 2895 2957 3687 336 Size: 611 93 74 190 62 730 1562 Fragments arranged by size: 1562 730 611 281 280 272 235 228 220 190 93 74 62 48 27 BspEI T'CCGG_A Cuts at: 1118 4133 1118 Size: 3015 1898 BspHI T'CATG_A Cuts at: 1559 4089 1559 Size: 2530 2383 BspMI ACCTGCnnnn'nnnn_ Cuts at: 710 1224 1245 1651 1664 1963 2177 2479 2860 Size: 514 21 406 13 299 214 302 381 Cuts at: 2860 710 Size: 2763 Fragments arranged by size: 2763 514 406 381 302 299 214 21 13 BsrBI CCG'CTC Cuts at: 50 3205 3259 4743 4908 50 Size: 3155 54 1484 165 55 Fragments arranged by size: 3155 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 176 1687 2825 176 Size: 1511 1138 2264 Fragments arranged by size: 2264 1511 1138 BsrFI r'CCGG_y Cuts at: 139 1419 2911 3092 3301 139 Size: 1280 1492 181 209 1751 Fragments arranged by size: 1751 1492 1280 209 181 BssSI C'ACGA_G Cuts at: 784 3184 3542 784 Size: 2400 358 2155 Fragments arranged by size: 2400 2155 358 BstAPI GCAn_nnn'nTGC Cuts at: 1107 1512 1632 2290 1107 Size: 405 120 658 3730 Fragments arranged by size: 3730 658 405 120 BstBI TT'CG_AA Cuts at: 3274 3274 Size: 4913 BstXI CCAn_nnnn'nTGG Cuts at: 417 1365 417 Size: 948 3965 BstYI r'GATC_y Cuts at: 1 974 1112 1193 1938 2405 2428 2763 3009 Size: 973 138 81 745 467 23 335 246 Cuts at: 3009 4021 4107 4119 4465 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 973 745 467 449 346 335 246 138 86 81 23 12 Bsu36I CC'TnA_GG Cuts at: 1520 1520 Size: 4913 BtgI C'CryG_G Cuts at: 791 911 791 Size: 120 4793 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2863 3044 3207 2863 Size: 181 163 4569 Fragments arranged by size: 4569 181 163 BtsI GCAGTG_nn' Cuts at: 324 2156 324 Size: 1832 3081 DraI TTT'AAA Cuts at: 1912 1912 Size: 4913 DraIII CAC_nnn'GTG Cuts at: 1734 1734 Size: 4913 DrdI GACnn_nn'nnGTC Cuts at: 1767 2619 3477 1767 Size: 852 858 3203 Fragments arranged by size: 3203 858 852 EaeI y'GGCC_r Cuts at: 141 270 1185 1421 2498 2672 3063 3090 4435 Size: 129 915 236 1077 174 391 27 1345 Cuts at: 4435 141 Size: 619 Fragments arranged by size: 1345 1077 915 619 391 236 174 129 27 EagI C'GGCC_G Cuts at: 270 2498 270 Size: 2228 2685 EarI CTCTTCn'nnn_ Cuts at: 2936 3146 2936 Size: 210 4703 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3121 3431 3577 4723 3121 Size: 310 146 1146 3311 Fragments arranged by size: 3311 1146 310 146 EcoICRI GAG'CTC Cuts at: 849 1398 1925 849 Size: 549 527 3837 Fragments arranged by size: 3837 549 527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 330 439 571 793 883 910 1261 1396 1510 Size: 109 132 222 90 27 351 135 114 Cuts at: 1510 1741 1765 1900 2738 3170 3917 4185 330 Size: 231 24 135 838 432 747 268 1058 Fragments arranged by size: 1058 838 747 432 351 268 231 222 135 135 132 114 109 90 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 1780 1894 1780 Size: 114 4799 EcoO109I rG'GnC_Cy Cuts at: 1803 1887 1899 2052 1803 Size: 84 12 153 4664 Fragments arranged by size: 4664 153 84 12 EcoRI G'AATT_C Cuts at: 1917 1917 Size: 4913 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 4913 FseI GG_CCGG'CC Cuts at: 143 143 Size: 4913 FspI TGC'GCA Cuts at: 2694 2694 Size: 4913 HaeII r_GCGC'y Cuts at: 753 2347 2595 3617 4596 753 Size: 1594 248 1022 979 1070 Fragments arranged by size: 1594 1070 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1252 1284 2755 2785 2787 2817 1252 Size: 32 1471 30 2 30 3348 Fragments arranged by size: 3348 1471 32 30 30 2 HincII GTy'rAC Cuts at: 1407 1952 1407 Size: 545 4368 KasI G'GCGC_C Cuts at: 2343 2591 2343 Size: 248 4665 KpnI G_GTAC'C Cuts at: 1933 1933 Size: 4913 MluI A'CGCG_T Cuts at: 4346 4346 Size: 4913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3584 3768 3584 Size: 184 4729 MscI TGG'CCA Cuts at: 2674 2674 Size: 4913 MslI CAynn'nnrTG Cuts at: 379 1658 1816 2902 3029 379 Size: 1279 158 1086 127 2263 Fragments arranged by size: 2263 1279 1086 158 127 MspA1I CmG'CkG Cuts at: 1202 1209 1308 1632 2024 2338 2698 3711 3956 Size: 7 99 324 392 314 360 1013 245 Cuts at: 3956 1202 Size: 2159 Fragments arranged by size: 2159 1013 392 360 324 314 245 99 7 NaeI GCC'GGC Cuts at: 141 1421 3094 141 Size: 1280 1673 1960 Fragments arranged by size: 1960 1673 1280 NarI GG'CG_CC Cuts at: 2344 2592 2344 Size: 248 4665 NgoMIV G'CCGG_C Cuts at: 139 1419 3092 139 Size: 1280 1673 1960 Fragments arranged by size: 1960 1673 1280 NspI r_CATG'y Cuts at: 1005 1269 1419 1966 2466 3373 1005 Size: 264 150 547 500 907 2545 Fragments arranged by size: 2545 907 547 500 264 150 PciI A'CATG_T Cuts at: 1001 1265 3369 1001 Size: 264 2104 2545 Fragments arranged by size: 2545 2104 264 PflMI CCAn_nnn'nTGG Cuts at: 150 582 150 Size: 432 4481 PfoI T'CCnGG_A Cuts at: 287 860 1745 1831 287 Size: 573 885 86 3369 Fragments arranged by size: 3369 885 573 86 PmeI GTTT'AAAC Cuts at: 1912 1912 Size: 4913 PmlI CAC'GTG Cuts at: 579 579 Size: 4913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 4052 Size: 32 4881 PpuMI rG'GwC_Cy Cuts at: 1803 1803 Size: 4913 PsiI TTA'TAA Cuts at: 644 644 Size: 4913 PstI C_TGCA'G Cuts at: 1112 1960 2645 1112 Size: 848 685 3380 Fragments arranged by size: 3380 848 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4913 PvuII CAG'CTG Cuts at: 1308 1632 2338 2698 1308 Size: 324 706 360 3523 Fragments arranged by size: 3523 706 360 324 RsrII CG'GwC_CG Cuts at: 3108 3108 Size: 4913 SacI G_AGCT'C Cuts at: 851 1400 1927 851 Size: 549 527 3837 Fragments arranged by size: 3837 549 527 SalI G'TCGA_C Cuts at: 1950 1950 Size: 4913 SapI GCTCTTCn'nnn_ Cuts at: 2936 3146 2936 Size: 210 4703 SbfI CC_TGCA'GG Cuts at: 1960 1960 Size: 4913 ScaI AGT'ACT Cuts at: 4430 4430 Size: 4913 SfcI C'TryA_G Cuts at: 33 1108 1698 1956 2641 3634 3825 33 Size: 1075 590 258 685 993 191 1121 Fragments arranged by size: 1121 1075 993 685 590 258 191 SfoI GGC'GCC Cuts at: 2345 2593 2345 Size: 248 4665 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4913 SmaI CCC'GGG Cuts at: 1935 1935 Size: 4913 SmlI C'TyrA_G Cuts at: 1216 1708 2068 2983 3475 3737 1216 Size: 492 360 915 492 262 2392 Fragments arranged by size: 2392 915 492 492 360 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4913 SphI G_CATG'C Cuts at: 1966 2466 1966 Size: 500 4413 StuI AGG'CCT Cuts at: 562 1027 562 Size: 465 4448 StyI C'CwwG_G Cuts at: 131 1292 1814 2047 2401 131 Size: 1161 522 233 354 2643 Fragments arranged by size: 2643 1161 522 354 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 248 277 409 674 937 1074 2496 2629 3088 Size: 29 132 265 263 137 1422 133 459 Cuts at: 3088 3297 248 Size: 209 1864 Fragments arranged by size: 1864 1422 459 265 263 209 137 133 132 29 TatI w'GTAC_w Cuts at: 1262 4428 1262 Size: 3166 1747 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4430 4462 4430 Size: 32 4881 Tth111I GACn'n_nGTC Cuts at: 2710 2710 Size: 4913 XbaI T'CTAG_A Cuts at: 1944 1944 Size: 4913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1821 2408 1821 Size: 587 4326 XhoI C'TCGA_G Cuts at: 1216 1216 Size: 4913 XmaI C'CCGG_G Cuts at: 1933 1933 Size: 4913 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AflII AhdI AloI ApaI AscI AvrII BbsI BfrBI BglI BmtI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI