Restriction map for
pF1KB8307
(Circular) (Six-base) MAPSORT of: pF1KB8307.seq Check: 4209 from: 1 to: 3467
pF1KB8307 3467 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1292 1724 2471 2739 1292
Size: 432 747 268 2020
Fragments arranged by size:
2020 747 432 268
AflIII A'CryG_T
Cuts at: 819 1923 2900 819
Size: 1104 977 1386
Fragments arranged by size:
1386 1104 977
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AlwNI CAG_nnn'CTG
Cuts at: 772 2339 772
Size: 1567 1900
ApaI G_GGCC'C
Cuts at: 135 135
Size: 3467
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 262 471 2820 3076 262
Size: 209 2349 256 653
Fragments arranged by size:
2349 653 256 209
AseI AT'TA_AT
Cuts at: 20 290 20
Size: 270 3197
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 487 487
Size: 3467
BamHI G'GATC_C
Cuts at: 492 492
Size: 3467
BanI G'GyrC_C
Cuts at: 483 897 1145 1180 483
Size: 414 248 35 2770
Fragments arranged by size:
2770 414 248 35
BanII G_rGCy'C
Cuts at: 135 481 1511 135
Size: 346 1030 2091
Fragments arranged by size:
2091 1030 346
BbeI G_GCGC'C
Cuts at: 901 1149 901
Size: 248 3219
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 987 987
Size: 3467
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 579 579
Size: 3467
Bme1580I G_kGCm'C
Cuts at: 135 1092 1185 2241 135
Size: 957 93 1056 1361
Fragments arranged by size:
1361 1056 957 93
BmrI ACTGGGnnnn_n'
Cuts at: 679 760 1095 3239 679
Size: 81 335 2144 907
Fragments arranged by size:
2144 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 442 281
Size: 161 3306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1522 2014 2312 643
Size: 879 492 298 1798
Fragments arranged by size:
1798 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3171 3171
Size: 3467
BsaAI yAC'GTr
Cuts at: 820 820
Size: 3467
BsaBI GATnn'nnATC
Cuts at: 6 305 981 3018 6
Size: 299 676 2037 455
Fragments arranged by size:
2037 676 455 299
BsaHI Gr'CG_yC
Cuts at: 898 1146 1848 3344 898
Size: 248 702 1496 1021
Fragments arranged by size:
1496 1021 702 248
BsaWI w'CCGG_w
Cuts at: 194 656 1177 1855 2129 2276 2687 194
Size: 462 521 678 274 147 411 974
Fragments arranged by size:
974 678 521 462 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 173 203 112
Size: 30 31 30 3376
Fragments arranged by size:
3376 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 308 308
Size: 3467
BseYI C'CCAG_C
Cuts at: 892 1650 2227 2723 892
Size: 758 577 496 1636
Fragments arranged by size:
1636 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1009 1055 2263 104
Size: 905 46 1208 1308
Fragments arranged by size:
1308 1208 905 46
BsiHKAI G_wGCw'C
Cuts at: 481 1259 1449 2241 481
Size: 778 190 792 1707
Fragments arranged by size:
1707 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 135 481 1092 1185 1259 1449 1511 2241 135
Size: 346 611 93 74 190 62 730 1361
Fragments arranged by size:
1361 730 611 346 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 2643 2643
Size: 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 731 1033 1414 517
Size: 214 302 381 2570
Fragments arranged by size:
2570 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1759 1813 3297 3462 50
Size: 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 1465 1646 1855 1465
Size: 181 209 3077
Fragments arranged by size:
3077 209 181
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 844 844
Size: 3467
BstBI TT'CG_AA
Cuts at: 1828 1828
Size: 3467
BstYI r'GATC_y
Cuts at: 1 492 959 982 1317 1563 2575 2661 2673
Size: 491 467 23 335 246 1012 86 12
Cuts at: 2673 3019 1
Size: 346 449
Fragments arranged by size:
1012 491 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 710 710
Size: 3467
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 173 1052 1226 1617 1644 2989 173
Size: 879 174 391 27 1345 651
Fragments arranged by size:
1345 879 651 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 227 326 458 1490 1700 227
Size: 99 132 1032 210 1994
Fragments arranged by size:
1994 1032 210 132 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 479 479
Size: 3467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 442 1292 1724 2471 2739 281
Size: 161 850 432 747 268 1009
Fragments arranged by size:
1009 850 747 432 268 161
EcoO109I rG'GnC_Cy
Cuts at: 132 606 132
Size: 474 2993
EcoRI G'AATT_C
Cuts at: 471 471
Size: 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 1486
Size: 3467
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 901 1149 2171 3150 901
Size: 248 1022 979 1218
Fragments arranged by size:
1218 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 1309 1339 1341 1371 173
Size: 32 1104 30 2 30 2269
Fragments arranged by size:
2269 1104 32 30 30 2
HincII GTy'rAC
Cuts at: 192 506 192
Size: 314 3153
KasI G'GCGC_C
Cuts at: 897 1145 897
Size: 248 3219
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2322 2138
Size: 184 3283
MscI TGG'CCA
Cuts at: 175 1228 175
Size: 1053 2414
MslI CAynn'nnrTG
Cuts at: 1456 1583 1456
Size: 127 3340
MspA1I CmG'CkG
Cuts at: 578 892 1252 2265 2510 578
Size: 314 360 1013 245 1535
Fragments arranged by size:
1535 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1648 1648
Size: 3467
NarI GG'CG_CC
Cuts at: 898 1146 898
Size: 248 3219
NgoMIV G'CCGG_C
Cuts at: 1646 1646
Size: 3467
NspI r_CATG'y
Cuts at: 520 1020 1927 520
Size: 500 907 2060
Fragments arranged by size:
2060 907 500
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PfoI T'CCnGG_A
Cuts at: 257 257
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PspOMI G'GGCC_C
Cuts at: 131 131
Size: 3467
PstI C_TGCA'G
Cuts at: 514 1199 514
Size: 685 2782
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 892 1252 892
Size: 360 3107
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 481 481
Size: 3467
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 458 1490 1700 458
Size: 1032 210 2225
Fragments arranged by size:
2225 1032 210
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 510 1195 2188 2379 33
Size: 477 685 993 191 1121
Fragments arranged by size:
1121 993 685 477 191
SfoI GGC'GCC
Cuts at: 899 1147 899
Size: 248 3219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 489 489
Size: 3467
SmlI C'TyrA_G
Cuts at: 622 1537 2029 2291 622
Size: 915 492 262 1798
Fragments arranged by size:
1798 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 520 1020 520
Size: 500 2967
StyI C'CwwG_G
Cuts at: 176 601 955 176
Size: 425 354 2688
Fragments arranged by size:
2688 425 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1050 1183 1642 1851 1050
Size: 133 459 209 2666
Fragments arranged by size:
2666 459 209 133
TatI w'GTAC_w
Cuts at: 2982 2982
Size: 3467
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 112 144 2984 3016 112
Size: 32 2840 32 563
Fragments arranged by size:
2840 563 32 32
Tth111I GACn'n_nGTC
Cuts at: 1264 1264
Size: 3467
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 962 962
Size: 3467
XmaI C'CCGG_G
Cuts at: 487 487
Size: 3467
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI