Restriction map for
pF1KB8313
(Circular) (Six-base) MAPSORT of: pF1KB8313.seq Check: 3267 from: 1 to: 4217
pF1KB8313 4217 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 665 665
Size: 4217
AccI GT'mk_AC
Cuts at: 962 1255 962
Size: 293 3924
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 2042 2474 3221 3489 262
Size: 1780 432 747 268 990
Fragments arranged by size:
1780 990 747 432 268
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 827 884 827
Size: 57 4160
AlwNI CAG_nnn'CTG
Cuts at: 306 909 1003 1050 1093 1522 3089 306
Size: 603 94 47 43 429 1567 1434
Fragments arranged by size:
1567 1434 603 429 94 47 43
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 1221 3570 3826 1221
Size: 2349 256 1612
Fragments arranged by size:
2349 1612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 265 359 579 1237 265
Size: 94 220 658 3245
Fragments arranged by size:
3245 658 220 94
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 1233 1647 1895 1930 1233
Size: 414 248 35 3520
Fragments arranged by size:
3520 414 248 35
BanII G_rGCy'C
Cuts at: 485 641 1231 2261 485
Size: 156 590 1030 2441
Fragments arranged by size:
2441 1030 590 156
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BciVI GTATCCnnnnn_n'
Cuts at: 2115 2876 4217 2115
Size: 761 1341 2115
Fragments arranged by size:
2115 1341 761
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglI GCCn_nnn'nGGC
Cuts at: 170 170
Size: 4217
BglII A'GATC_T
Cuts at: 1145 1732 3769 1145
Size: 587 2037 1593
Fragments arranged by size:
2037 1593 587
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 1842 1935 2991 1842
Size: 93 1056 3068
Fragments arranged by size:
3068 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1429 1510 1845 3989 1429
Size: 81 335 2144 1657
Fragments arranged by size:
2144 1657 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 660 2584 660
Size: 1924 2293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 555 555
Size: 4217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1393 2272 2764 3062 472
Size: 921 879 492 298 1627
Fragments arranged by size:
1627 921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1111 3921 1111
Size: 2810 1407
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 1731 3768 6
Size: 1725 2037 455
Fragments arranged by size:
2037 1725 455
BsaHI Gr'CG_yC
Cuts at: 1648 1896 2598 4094 1648
Size: 248 702 1496 1771
Fragments arranged by size:
1771 1496 702 248
BsaWI w'CCGG_w
Cuts at: 548 1406 1927 2605 2879 3026 3437 548
Size: 858 521 678 274 147 411 1328
Fragments arranged by size:
1328 858 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 703 781 784 787 475
Size: 228 78 3 3 3905
Fragments arranged by size:
3905 228 78 3 3
BseYI C'CCAG_C
Cuts at: 441 630 975 1151 1642 2400 2977 3473 441
Size: 189 345 176 491 758 577 496 1185
Fragments arranged by size:
1185 758 577 496 491 345 189 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 333
Size: 4217
BsiEI CG_ry'CG
Cuts at: 104 165 1759 1805 3013 104
Size: 61 1594 46 1208 1308
Fragments arranged by size:
1594 1308 1208 61 46
BsiHKAI G_wGCw'C
Cuts at: 485 571 596 641 1231 2009 2199 2991 485
Size: 86 25 45 590 778 190 792 1711
Fragments arranged by size:
1711 792 778 590 190 86 45 25
Bsp1286I G_dGCh'C
Cuts at: 485 571 596 641 1231 1842 1935 2009 2199
Size: 86 25 45 590 611 93 74 190
Cuts at: 2199 2261 2991 485
Size: 62 730 1711
Fragments arranged by size:
1711 730 611 590 190 93 86 74
62 45 25
BspEI T'CCGG_A
Cuts at: 548 3437 548
Size: 2889 1328
BspHI T'CATG_A
Cuts at: 1172 3393 1172
Size: 2221 1996
BspMI ACCTGCnnnn'nnnn_
Cuts at: 665 1267 1481 1783 2164 665
Size: 602 214 302 381 2718
Fragments arranged by size:
2718 602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2509 2563 4047 4212 50
Size: 2459 54 1484 165 55
Fragments arranged by size:
2459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2129 2129
Size: 4217
BsrFI r'CCGG_y
Cuts at: 2215 2396 2605 2215
Size: 181 209 3827
Fragments arranged by size:
3827 209 181
BssSI C'ACGA_G
Cuts at: 448 2488 2846 448
Size: 2040 358 1819
Fragments arranged by size:
2040 1819 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstYI r'GATC_y
Cuts at: 1 338 422 1145 1242 1709 1732 2067 2313
Size: 337 84 723 97 467 23 335 246
Cuts at: 2313 3325 3411 3423 3769 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 723 467 449 346 337 335 246
97 86 84 23 12
BtgI C'CryG_G
Cuts at: 107 165 688 1112 107
Size: 58 523 424 3212
Fragments arranged by size:
3212 523 424 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 373 745 1460 373
Size: 372 715 3130
Fragments arranged by size:
3130 715 372
DraI TTT'AAA
Cuts at: 1216 1216
Size: 4217
DrdI GACnn_nn'nnGTC
Cuts at: 1923 2781 1923
Size: 858 3359
EaeI y'GGCC_r
Cuts at: 162 1802 1976 2367 2394 3739 162
Size: 1640 174 391 27 1345 640
Fragments arranged by size:
1640 1345 640 391 174 27
EagI C'GGCC_G
Cuts at: 162 1802 162
Size: 1640 2577
EarI CTCTTCn'nnn_
Cuts at: 646 2240 2450 646
Size: 1594 210 2413
Fragments arranged by size:
2413 1594 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 799 2425 2735 2881 4027 799
Size: 1626 310 146 1146 989
Fragments arranged by size:
1626 1146 989 310 146
EcoICRI GAG'CTC
Cuts at: 483 639 1229 483
Size: 156 590 3471
Fragments arranged by size:
3471 590 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 555 2042 2474 3221 3489 262
Size: 293 1487 432 747 268 990
Fragments arranged by size:
1487 990 747 432 293 268
EcoO109I rG'GnC_Cy
Cuts at: 246 405 1000 1356 246
Size: 159 595 356 3107
Fragments arranged by size:
3107 595 356 159
EcoRI G'AATT_C
Cuts at: 1221 1221
Size: 4217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 1998 1998
Size: 4217
HaeII r_GCGC'y
Cuts at: 996 1651 1899 2921 3900 996
Size: 655 248 1022 979 1313
Fragments arranged by size:
1313 1022 979 655 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 2059 2089 2091 2121 166
Size: 32 1861 30 2 30 2262
Fragments arranged by size:
2262 1861 32 30 30 2
HincII GTy'rAC
Cuts at: 1256 1256
Size: 4217
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 950 2888 3072 950
Size: 1938 184 2095
Fragments arranged by size:
2095 1938 184
MscI TGG'CCA
Cuts at: 1978 1978
Size: 4217
MslI CAynn'nnrTG
Cuts at: 2206 2333 2206
Size: 127 4090
MspA1I CmG'CkG
Cuts at: 801 1328 1642 2002 3015 3260 801
Size: 527 314 360 1013 245 1758
Fragments arranged by size:
1758 1013 527 360 314 245
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NcoI C'CATG_G
Cuts at: 107 107
Size: 4217
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 705 1270 1770 2677 705
Size: 565 500 907 2245
Fragments arranged by size:
2245 907 565 500
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PpuMI rG'GwC_Cy
Cuts at: 246 405 246
Size: 159 4058
PspXI vC'TCGA_Gb
Cuts at: 265 579 265
Size: 314 3903
PstI C_TGCA'G
Cuts at: 380 662 806 1264 1949 380
Size: 282 144 458 685 2648
Fragments arranged by size:
2648 685 458 282 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 801 1642 2002 801
Size: 841 360 3016
Fragments arranged by size:
3016 841 360
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 485 641 1231 485
Size: 156 590 3471
Fragments arranged by size:
3471 590 156
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SanDI GG'GwC_CC
Cuts at: 405 405
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 646 2240 2450 646
Size: 1594 210 2413
Fragments arranged by size:
2413 1594 210
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 844 3734 844
Size: 2890 1327
SfcI C'TryA_G
Cuts at: 33 376 658 802 1059 1260 1945 2938 3129
Size: 343 282 144 257 201 685 993 191
Cuts at: 3129 33
Size: 1121
Fragments arranged by size:
1121 993 685 343 282 257 201 191
144
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 361 1239 361
Size: 878 3339
SmlI C'TyrA_G
Cuts at: 265 487 579 1372 2287 2779 3041 265
Size: 222 92 793 915 492 262 1441
Fragments arranged by size:
1441 915 793 492 262 222 92
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
StuI AGG'CCT
Cuts at: 625 625
Size: 4217
StyI C'CwwG_G
Cuts at: 107 1351 1705 107
Size: 1244 354 2619
Fragments arranged by size:
2619 1244 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 554 1800 1933 2392 2601 554
Size: 1246 133 459 209 2170
Fragments arranged by size:
2170 1246 459 209 133
TatI w'GTAC_w
Cuts at: 842 3732 842
Size: 2890 1327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 295 327 3734 3766 295
Size: 32 3407 32 746
Fragments arranged by size:
3407 746 32 32
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 219 1712 219
Size: 1493 2724
XhoI C'TCGA_G
Cuts at: 265 579 265
Size: 314 3903
XmaI C'CCGG_G
Cuts at: 359 1237 359
Size: 878 3339
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI