Restriction map for
pF1KB8314
(Circular) (Six-base) MAPSORT of: pF1KB8314.seq Check: 4648 from: 1 to: 4217
pF1KB8314 4217 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 326 326
Size: 4217
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 492 751 2042 2474 3221 3489 370
Size: 122 259 1291 432 747 268 1098
Fragments arranged by size:
1291 1098 747 432 268 259 122
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AleI CACnn'nnGTG
Cuts at: 1048 1048
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 156 1522 3089 156
Size: 1366 1567 1284
Fragments arranged by size:
1567 1366 1284
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 1221 3570 3826 1221
Size: 2349 256 1612
Fragments arranged by size:
2349 1612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 1237 1237
Size: 4217
AvrII C'CTAG_G
Cuts at: 885 885
Size: 4217
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 122 627 981 1233 1647 1895 1930 122
Size: 505 354 252 414 248 35 2409
Fragments arranged by size:
2409 505 414 354 252 248 35
BanII G_rGCy'C
Cuts at: 422 599 653 953 995 1231 2261 422
Size: 177 54 300 42 236 1030 2378
Fragments arranged by size:
2378 1030 300 236 177 54 42
BbeI G_GCGC'C
Cuts at: 126 1651 1899 126
Size: 1525 248 2444
Fragments arranged by size:
2444 1525 248
BciVI GTATCCnnnnn_n'
Cuts at: 2115 2876 4217 2115
Size: 761 1341 2115
Fragments arranged by size:
2115 1341 761
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglI GCCn_nnn'nGGC
Cuts at: 120 990 120
Size: 870 3347
BglII A'GATC_T
Cuts at: 368 482 1732 3769 368
Size: 114 1250 2037 816
Fragments arranged by size:
2037 1250 816 114
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 362 632 986 1136 1842 1935 2991 362
Size: 270 354 150 706 93 1056 1588
Fragments arranged by size:
1588 1056 706 354 270 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 1149 1429 1510 1845 3989 1149
Size: 280 81 335 2144 1377
Fragments arranged by size:
2144 1377 335 280 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 712 734 865 997 430
Size: 282 22 131 132 3650
Fragments arranged by size:
3650 282 132 131 22
Bpu10I CC'TnA_GC
Cuts at: 145 925 145
Size: 780 3437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1393 2272 2764 3062 988
Size: 405 879 492 298 2143
Fragments arranged by size:
2143 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 448 787 3921 448
Size: 339 3134 744
Fragments arranged by size:
3134 744 339
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 1731 3768 6
Size: 1725 2037 455
Fragments arranged by size:
2037 1725 455
BsaHI Gr'CG_yC
Cuts at: 123 251 1648 1896 2598 4094 123
Size: 128 1397 248 702 1496 246
Fragments arranged by size:
1496 1397 702 248 246 128
BsaWI w'CCGG_w
Cuts at: 653 1406 1927 2605 2879 3026 3437 653
Size: 753 521 678 274 147 411 1433
Fragments arranged by size:
1433 753 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 397 427 469 499 397
Size: 30 42 30 4115
Fragments arranged by size:
4115 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1000 1000
Size: 4217
BseYI C'CCAG_C
Cuts at: 580 879 1642 2400 2977 3473 580
Size: 299 763 758 577 496 1324
Fragments arranged by size:
1324 763 758 577 496 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1168 1012
Size: 156 4061
BsiEI CG_ry'CG
Cuts at: 104 1759 1805 3013 104
Size: 1655 46 1208 1308
Fragments arranged by size:
1655 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 337 422 599 953 1231 2009 2199 2991 337
Size: 85 177 354 278 778 190 792 1563
Fragments arranged by size:
1563 792 778 354 278 190 177 85
Bsp1286I G_dGCh'C
Cuts at: 337 362 422 599 632 653 953 986 995
Size: 25 60 177 33 21 300 33 9
Cuts at: 995 1136 1231 1842 1935 2009 2199 2261 2991
Size: 141 95 611 93 74 190 62 730
Cuts at: 2991 337
Size: 1563
Fragments arranged by size:
1563 730 611 300 190 177 141 95
93 74 62 60 33 33 25 21
9
BspEI T'CCGG_A
Cuts at: 653 3437 653
Size: 2784 1433
BspHI T'CATG_A
Cuts at: 209 3393 209
Size: 3184 1033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 404 752 860 1267 1481 1783 2164 326
Size: 78 348 108 407 214 302 381 2379
Fragments arranged by size:
2379 407 381 348 302 214 108 78
BsrBI CCG'CTC
Cuts at: 50 2509 2563 4047 4212 50
Size: 2459 54 1484 165 55
Fragments arranged by size:
2459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2129 2129
Size: 4217
BsrFI r'CCGG_y
Cuts at: 1152 2215 2396 2605 1152
Size: 1063 181 209 2764
Fragments arranged by size:
2764 1063 209 181
BsrGI T'GTAC_A
Cuts at: 872 872
Size: 4217
BssSI C'ACGA_G
Cuts at: 127 2488 2846 127
Size: 2361 358 1498
Fragments arranged by size:
2361 1498 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 390 588 843 390
Size: 198 255 3764
Fragments arranged by size:
3764 255 198
BstYI r'GATC_y
Cuts at: 1 368 482 491 563 1242 1709 1732 2067
Size: 367 114 9 72 679 467 23 335
Cuts at: 2067 2313 3325 3411 3423 3769 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 679 467 449 367 346 335 246
114 86 72 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 246 987 246
Size: 741 3476
BtgI C'CryG_G
Cuts at: 1073 1073
Size: 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 309 514 1460 309
Size: 205 946 3066
Fragments arranged by size:
3066 946 205
DraI TTT'AAA
Cuts at: 1216 1216
Size: 4217
DraIII CAC_nnn'GTG
Cuts at: 558 558
Size: 4217
DrdI GACnn_nn'nnGTC
Cuts at: 1923 2781 1923
Size: 858 3359
EaeI y'GGCC_r
Cuts at: 974 1154 1184 1802 1976 2367 2394 3739 974
Size: 180 30 618 174 391 27 1345 1452
Fragments arranged by size:
1452 1345 618 391 180 174 30 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 1123 2240 2450 1123
Size: 1117 210 2890
Fragments arranged by size:
2890 1117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 2735 2881 4027 2425
Size: 310 146 1146 2615
Fragments arranged by size:
2615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 420 597 951 1229 420
Size: 177 354 278 3408
Fragments arranged by size:
3408 354 278 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 430 492 712 734 751 865 997 2042
Size: 60 62 220 22 17 114 132 1045
Cuts at: 2042 2474 3221 3489 370
Size: 432 747 268 1098
Fragments arranged by size:
1098 1045 747 432 268 220 132 114
62 60 22 17
EcoNI CCTnn'n_nnAGG
Cuts at: 148 148
Size: 4217
EcoO109I rG'GnC_Cy
Cuts at: 153 849 1356 153
Size: 696 507 3014
Fragments arranged by size:
3014 696 507
EcoRI G'AATT_C
Cuts at: 1221 1221
Size: 4217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 450 2236 450
Size: 1786 2431
FspI TGC'GCA
Cuts at: 748 1998 748
Size: 1250 2967
HaeII r_GCGC'y
Cuts at: 126 239 290 1651 1899 2921 3900 126
Size: 113 51 1361 248 1022 979 443
Fragments arranged by size:
1361 1022 979 443 248 113 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 2059 2089 2091 2121 250
Size: 32 1777 30 2 30 2346
Fragments arranged by size:
2346 1777 32 30 30 2
HincII GTy'rAC
Cuts at: 1059 1256 1059
Size: 197 4020
HindIII A'AGCT_T
Cuts at: 227 227
Size: 4217
KasI G'GCGC_C
Cuts at: 122 1647 1895 122
Size: 1525 248 2444
Fragments arranged by size:
2444 1525 248
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 1058 2888 3072 362
Size: 696 1830 184 1507
Fragments arranged by size:
1830 1507 696 184
MscI TGG'CCA
Cuts at: 976 1186 1978 976
Size: 210 792 3215
Fragments arranged by size:
3215 792 210
MslI CAynn'nnrTG
Cuts at: 673 1048 2206 2333 673
Size: 375 1158 127 2557
Fragments arranged by size:
2557 1158 375 127
MspA1I CmG'CkG
Cuts at: 647 1166 1328 1642 2002 3015 3260 647
Size: 519 162 314 360 1013 245 1604
Fragments arranged by size:
1604 1013 519 360 314 245 162
NaeI GCC'GGC
Cuts at: 1154 2398 1154
Size: 1244 2973
NarI GG'CG_CC
Cuts at: 123 1648 1896 123
Size: 1525 248 2444
Fragments arranged by size:
2444 1525 248
NcoI C'CATG_G
Cuts at: 1073 1073
Size: 4217
NgoMIV G'CCGG_C
Cuts at: 1152 2396 1152
Size: 1244 2973
NspI r_CATG'y
Cuts at: 1270 1770 2677 1270
Size: 500 907 2810
Fragments arranged by size:
2810 907 500
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PflMI CCAn_nnn'nTGG
Cuts at: 558 558
Size: 4217
PfoI T'CCnGG_A
Cuts at: 254 944 254
Size: 690 3527
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PpuMI rG'GwC_Cy
Cuts at: 849 849
Size: 4217
PshAI GACnn'nnGTC
Cuts at: 344 1067 344
Size: 723 3494
PstI C_TGCA'G
Cuts at: 401 530 1264 1949 401
Size: 129 734 685 2669
Fragments arranged by size:
2669 734 685 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4217
PvuII CAG'CTG
Cuts at: 1642 2002 1642
Size: 360 3857
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 422 599 953 1231 422
Size: 177 354 278 3408
Fragments arranged by size:
3408 354 278 177
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 2240 2450 2240
Size: 210 4007
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SexAI A'CCwGG_T
Cuts at: 554 554
Size: 4217
SfcI C'TryA_G
Cuts at: 33 282 397 526 1260 1945 2938 3129 33
Size: 249 115 129 734 685 993 191 1121
Fragments arranged by size:
1121 993 734 685 249 191 129 115
SfoI GGC'GCC
Cuts at: 124 1649 1897 124
Size: 1525 248 2444
Fragments arranged by size:
2444 1525 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 1003 1372 2287 2779 3041 1003
Size: 369 915 492 262 2179
Fragments arranged by size:
2179 915 492 369 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
StuI AGG'CCT
Cuts at: 505 505
Size: 4217
StyI C'CwwG_G
Cuts at: 632 885 1073 1351 1705 632
Size: 253 188 278 354 3144
Fragments arranged by size:
3144 354 278 253 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 291 1042 1800 1933 2392 2601 291
Size: 751 758 133 459 209 1907
Fragments arranged by size:
1907 758 751 459 209 133
TatI w'GTAC_w
Cuts at: 872 3732 872
Size: 2860 1357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3734 3766 3734
Size: 32 4185
Tth111I GACn'n_nGTC
Cuts at: 268 2014 268
Size: 1746 2471
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 552 588 1712 552
Size: 36 1124 3057
Fragments arranged by size:
3057 1124 36
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
XmnI GAAnn'nnTTC
Cuts at: 505 505
Size: 4217
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI