(Circular) (Six-base) MAPSORT of: pF1KB8320.seq Check: 4496 from: 1 to: 3500 pF1KB8320 3500 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 538 538 Size: 3500 Acc65I G'GTAC_C Cuts at: 516 516 Size: 3500 AclI AA'CG_TT Cuts at: 3340 3340 Size: 3500 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1325 1757 2504 2772 1325 Size: 432 747 268 2053 Fragments arranged by size: 2053 747 432 268 AflIII A'CryG_T Cuts at: 852 1956 2933 852 Size: 1104 977 1419 Fragments arranged by size: 1419 1104 977 AgeI A'CCGG_T Cuts at: 1888 1888 Size: 3500 AlwNI CAG_nnn'CTG Cuts at: 805 2372 805 Size: 1567 1933 ApaLI G'TGCA_C Cuts at: 2270 2270 Size: 3500 ApoI r'AATT_y Cuts at: 301 320 452 504 2853 3109 301 Size: 19 132 52 2349 256 692 Fragments arranged by size: 2349 692 256 132 52 19 AseI AT'TA_AT Cuts at: 20 20 Size: 3500 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3500 AvaI C'yCGr_G Cuts at: 520 520 Size: 3500 BamHI G'GATC_C Cuts at: 525 525 Size: 3500 BanI G'GyrC_C Cuts at: 516 930 1178 1213 516 Size: 414 248 35 2803 Fragments arranged by size: 2803 414 248 35 BanII G_rGCy'C Cuts at: 514 1544 514 Size: 1030 2470 BbeI G_GCGC'C Cuts at: 934 1182 934 Size: 248 3252 BciVI GTATCCnnnnn_n' Cuts at: 1398 2159 3500 1398 Size: 761 1341 1398 Fragments arranged by size: 1398 1341 761 BclI T'GATC_A Cuts at: 1020 1020 Size: 3500 BglII A'GATC_T Cuts at: 1015 3052 1015 Size: 2037 1463 BlpI GC'TnA_GC Cuts at: 612 612 Size: 3500 Bme1580I G_kGCm'C Cuts at: 1125 1218 2274 1125 Size: 93 1056 2351 Fragments arranged by size: 2351 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 712 793 1128 3272 712 Size: 81 335 2144 940 Fragments arranged by size: 2144 940 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1867 1867 Size: 3500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 449 449 Size: 3500 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 676 1555 2047 2345 676 Size: 879 492 298 1831 Fragments arranged by size: 1831 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3204 3204 Size: 3500 BsaAI yAC'GTr Cuts at: 853 853 Size: 3500 BsaBI GATnn'nnATC Cuts at: 6 1014 3051 6 Size: 1008 2037 455 Fragments arranged by size: 2037 1008 455 BsaHI Gr'CG_yC Cuts at: 931 1179 1881 3377 931 Size: 248 702 1496 1054 Fragments arranged by size: 1496 1054 702 248 BsaWI w'CCGG_w Cuts at: 689 1210 1888 2162 2309 2720 689 Size: 521 678 274 147 411 1469 Fragments arranged by size: 1469 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 925 1683 2260 2756 925 Size: 758 577 496 1669 Fragments arranged by size: 1669 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1042 1088 2296 104 Size: 938 46 1208 1308 Fragments arranged by size: 1308 1208 938 46 BsiHKAI G_wGCw'C Cuts at: 463 514 1292 1482 2274 463 Size: 51 778 190 792 1689 Fragments arranged by size: 1689 792 778 190 51 BsmI GAATG_Cn' Cuts at: 354 354 Size: 3500 Bsp1286I G_dGCh'C Cuts at: 463 514 1125 1218 1292 1482 1544 2274 463 Size: 51 611 93 74 190 62 730 1689 Fragments arranged by size: 1689 730 611 190 93 74 62 51 BspEI T'CCGG_A Cuts at: 2720 2720 Size: 3500 BspHI T'CATG_A Cuts at: 374 2676 374 Size: 2302 1198 BspMI ACCTGCnnnn'nnnn_ Cuts at: 550 764 1066 1447 550 Size: 214 302 381 2603 Fragments arranged by size: 2603 381 302 214 BsrBI CCG'CTC Cuts at: 50 1792 1846 3330 3495 50 Size: 1742 54 1484 165 55 Fragments arranged by size: 1742 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1412 1412 Size: 3500 BsrFI r'CCGG_y Cuts at: 1498 1679 1888 1498 Size: 181 209 3110 Fragments arranged by size: 3110 209 181 BssSI C'ACGA_G Cuts at: 1771 2129 1771 Size: 358 3142 BstAPI GCAn_nnn'nTGC Cuts at: 877 877 Size: 3500 BstBI TT'CG_AA Cuts at: 1861 1861 Size: 3500 BstYI r'GATC_y Cuts at: 1 525 992 1015 1350 1596 2608 2694 2706 Size: 524 467 23 335 246 1012 86 12 Cuts at: 2706 3052 1 Size: 346 449 Fragments arranged by size: 1012 524 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1450 1631 1794 1450 Size: 181 163 3156 Fragments arranged by size: 3156 181 163 BtsI GCAGTG_nn' Cuts at: 743 743 Size: 3500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 224 259 224 Size: 35 3465 DraI TTT'AAA Cuts at: 499 499 Size: 3500 DrdI GACnn_nn'nnGTC Cuts at: 1206 2064 1206 Size: 858 2642 EaeI y'GGCC_r Cuts at: 1085 1259 1650 1677 3022 1085 Size: 174 391 27 1345 1563 Fragments arranged by size: 1563 1345 391 174 27 EagI C'GGCC_G Cuts at: 1085 1085 Size: 3500 EarI CTCTTCn'nnn_ Cuts at: 1523 1733 1523 Size: 210 3290 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1708 2018 2164 3310 1708 Size: 310 146 1146 1898 Fragments arranged by size: 1898 1146 310 146 EcoICRI GAG'CTC Cuts at: 512 512 Size: 3500 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 449 1325 1757 2504 2772 449 Size: 876 432 747 268 1177 Fragments arranged by size: 1177 876 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 409 639 409 Size: 230 3270 EcoRI G'AATT_C Cuts at: 320 504 320 Size: 184 3316 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1519 1519 Size: 3500 FspI TGC'GCA Cuts at: 1281 1281 Size: 3500 HaeII r_GCGC'y Cuts at: 934 1182 2204 3183 934 Size: 248 1022 979 1251 Fragments arranged by size: 1251 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1342 1372 1374 1404 1342 Size: 30 2 30 3438 Fragments arranged by size: 3438 30 30 2 HincII GTy'rAC Cuts at: 539 539 Size: 3500 HindIII A'AGCT_T Cuts at: 289 289 Size: 3500 KasI G'GCGC_C Cuts at: 930 1178 930 Size: 248 3252 KpnI G_GTAC'C Cuts at: 520 520 Size: 3500 MfeI C'AATT_G Cuts at: 236 236 Size: 3500 MluI A'CGCG_T Cuts at: 2933 2933 Size: 3500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 153 2171 2355 153 Size: 2018 184 1298 Fragments arranged by size: 2018 1298 184 MscI TGG'CCA Cuts at: 1261 1261 Size: 3500 MslI CAynn'nnrTG Cuts at: 379 1489 1616 379 Size: 1110 127 2263 Fragments arranged by size: 2263 1110 127 MspA1I CmG'CkG Cuts at: 388 424 611 925 1285 2298 2543 388 Size: 36 187 314 360 1013 245 1345 Fragments arranged by size: 1345 1013 360 314 245 187 36 NaeI GCC'GGC Cuts at: 1681 1681 Size: 3500 NarI GG'CG_CC Cuts at: 931 1179 931 Size: 248 3252 NgoMIV G'CCGG_C Cuts at: 1679 1679 Size: 3500 NspI r_CATG'y Cuts at: 553 1053 1960 553 Size: 500 907 2093 Fragments arranged by size: 2093 907 500 PciI A'CATG_T Cuts at: 1956 1956 Size: 3500 PfoI T'CCnGG_A Cuts at: 411 411 Size: 3500 PmeI GTTT'AAAC Cuts at: 499 499 Size: 3500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2639 2671 2639 Size: 32 3468 PpuMI rG'GwC_Cy Cuts at: 409 409 Size: 3500 PstI C_TGCA'G Cuts at: 547 1232 547 Size: 685 2815 PvuI CG_AT'CG Cuts at: 104 104 Size: 3500 PvuII CAG'CTG Cuts at: 388 424 925 1285 388 Size: 36 501 360 2603 Fragments arranged by size: 2603 501 360 36 RsrII CG'GwC_CG Cuts at: 1695 1695 Size: 3500 SacI G_AGCT'C Cuts at: 514 514 Size: 3500 SalI G'TCGA_C Cuts at: 537 537 Size: 3500 SanDI GG'GwC_CC Cuts at: 409 409 Size: 3500 SapI GCTCTTCn'nnn_ Cuts at: 1523 1733 1523 Size: 210 3290 SbfI CC_TGCA'GG Cuts at: 547 547 Size: 3500 ScaI AGT'ACT Cuts at: 3017 3017 Size: 3500 SfcI C'TryA_G Cuts at: 33 263 543 1228 2221 2412 33 Size: 230 280 685 993 191 1121 Fragments arranged by size: 1121 993 685 280 230 191 SfoI GGC'GCC Cuts at: 932 1180 932 Size: 248 3252 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3500 SmaI CCC'GGG Cuts at: 522 522 Size: 3500 SmlI C'TyrA_G Cuts at: 655 1570 2062 2324 655 Size: 915 492 262 1831 Fragments arranged by size: 1831 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3500 SphI G_CATG'C Cuts at: 553 1053 553 Size: 500 3000 StyI C'CwwG_G Cuts at: 315 634 988 315 Size: 319 354 2827 Fragments arranged by size: 2827 354 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1083 1216 1675 1884 1083 Size: 133 459 209 2699 Fragments arranged by size: 2699 459 209 133 TatI w'GTAC_w Cuts at: 3015 3015 Size: 3500 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3017 3049 3017 Size: 32 3468 Tth111I GACn'n_nGTC Cuts at: 1297 1297 Size: 3500 XbaI T'CTAG_A Cuts at: 531 531 Size: 3500 XcmI CCAnnnn_n'nnnnTGG Cuts at: 995 995 Size: 3500 XmaI C'CCGG_G Cuts at: 520 520 Size: 3500 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI