Restriction map for
pF1KB8322
(Circular) (Six-base) MAPSORT of: pF1KB8322.seq Check: 7638 from: 1 to: 5117
pF1KB8322 5117 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1531 2155 1531
Size: 624 4493
Acc65I G'GTAC_C
Cuts at: 2133 2133
Size: 5117
AclI AA'CG_TT
Cuts at: 4957 4957
Size: 5117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 1449 2942 3374 4121 4389 129
Size: 1320 1493 432 747 268 857
Fragments arranged by size:
1493 1320 857 747 432 268
AfeI AGC'GCT
Cuts at: 664 769 787 1813 664
Size: 105 18 1026 3968
Fragments arranged by size:
3968 1026 105 18
AflIII A'CryG_T
Cuts at: 632 1277 1676 1926 2469 3573 4550 632
Size: 645 399 250 543 1104 977 1199
Fragments arranged by size:
1199 1104 977 645 543 399 250
AgeI A'CCGG_T
Cuts at: 3505 3505
Size: 5117
AhdI GACnn_n'nnGTC
Cuts at: 570 570
Size: 5117
AleI CACnn'nnGTG
Cuts at: 197 1691 1777 197
Size: 1494 86 3537
Fragments arranged by size:
3537 1494 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 261 172
Size: 89 5028
AlwNI CAG_nnn'CTG
Cuts at: 930 1482 1566 1718 2422 3989 930
Size: 552 84 152 704 1567 2058
Fragments arranged by size:
2058 1567 704 552 152 84
ApaLI G'TGCA_C
Cuts at: 453 954 1626 1840 3887 453
Size: 501 672 214 2047 1683
Fragments arranged by size:
2047 1683 672 501 214
ApoI r'AATT_y
Cuts at: 2121 4470 4726 2121
Size: 2349 256 2512
Fragments arranged by size:
2512 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5117
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
AvaI C'yCGr_G
Cuts at: 160 492 507 535 805 1017 1114 2137 160
Size: 332 15 28 270 212 97 1023 3140
Fragments arranged by size:
3140 1023 332 270 212 97 28 15
BamHI G'GATC_C
Cuts at: 497 2142 497
Size: 1645 3472
BanI G'GyrC_C
Cuts at: 982 1039 1981 2133 2547 2795 2830 982
Size: 57 942 152 414 248 35 3269
Fragments arranged by size:
3269 942 414 248 152 57 35
BanII G_rGCy'C
Cuts at: 998 1831 2131 3161 998
Size: 833 300 1030 2954
Fragments arranged by size:
2954 1030 833 300
BbeI G_GCGC'C
Cuts at: 2551 2799 2551
Size: 248 4869
BbsI GAAGACnn'nnnn_
Cuts at: 743 968 743
Size: 225 4892
BbvCI CC'TCA_GC
Cuts at: 1139 1139
Size: 5117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 444 478 1105 1139 444
Size: 34 627 34 4422
Fragments arranged by size:
4422 627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3015 3776 5117 3015
Size: 761 1341 3015
Fragments arranged by size:
3015 1341 761
BclI T'GATC_A
Cuts at: 2637 2637
Size: 5117
BfrBI ATG'CAT
Cuts at: 2097 2097
Size: 5117
BglI GCCn_nnn'nGGC
Cuts at: 2000 2000
Size: 5117
BglII A'GATC_T
Cuts at: 2632 4669 2632
Size: 2037 3080
BlpI GC'TnA_GC
Cuts at: 2229 2229
Size: 5117
Bme1580I G_kGCm'C
Cuts at: 457 532 958 985 1630 1844 1986 2742 2835
Size: 75 426 27 645 214 142 756 93
Cuts at: 2835 3891 457
Size: 1056 1683
Fragments arranged by size:
1683 1056 756 645 426 214 142 93
75 27
BmgBI CAC'GTC
Cuts at: 548 1280 548
Size: 732 4385
BmrI ACTGGGnnnn_n'
Cuts at: 1494 2329 2410 2745 4889 1494
Size: 835 81 335 2144 1722
Fragments arranged by size:
2144 1722 835 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1068 1373 3484 1068
Size: 305 2111 2701
Fragments arranged by size:
2701 2111 305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1939 1939
Size: 5117
Bpu10I CC'TnA_GC
Cuts at: 1139 1139
Size: 5117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2293 3172 3664 3962 2293
Size: 879 492 298 3448
Fragments arranged by size:
3448 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 157 387 802 4821 157
Size: 230 415 4019 453
Fragments arranged by size:
4019 453 415 230
BsaAI yAC'GTr
Cuts at: 1929 2470 1929
Size: 541 4576
BsaBI GATnn'nnATC
Cuts at: 6 2631 4668 6
Size: 2625 2037 455
Fragments arranged by size:
2625 2037 455
BsaHI Gr'CG_yC
Cuts at: 1022 2548 2796 3498 4994 1022
Size: 1526 248 702 1496 1145
Fragments arranged by size:
1526 1496 1145 702 248
BsaWI w'CCGG_w
Cuts at: 1185 2306 2827 3505 3779 3926 4337 1185
Size: 1121 521 678 274 147 411 1965
Fragments arranged by size:
1965 1121 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 622 652 1051 1081 2023 2053 622
Size: 30 399 30 942 30 3686
Fragments arranged by size:
3686 942 399 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 964 1020 964
Size: 56 5061
BseYI C'CCAG_C
Cuts at: 742 1307 1430 1517 2049 2542 3300 3877 4373
Size: 565 123 87 532 493 758 577 496
Cuts at: 4373 742
Size: 1486
Fragments arranged by size:
1486 758 577 565 532 496 493 123
87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 943 1526 1921 1998 352
Size: 591 583 395 77 3471
Fragments arranged by size:
3471 591 583 395 77
BsiEI CG_ry'CG
Cuts at: 104 416 2659 2705 3913 104
Size: 312 2243 46 1208 1308
Fragments arranged by size:
2243 1308 1208 312 46
BsiHKAI G_wGCw'C
Cuts at: 161 457 640 958 1630 1831 1844 2131 2909
Size: 296 183 318 672 201 13 287 778
Cuts at: 2909 3099 3891 161
Size: 190 792 1387
Fragments arranged by size:
1387 792 778 672 318 296 287 201
190 183 13
BsmI GAATG_Cn'
Cuts at: 119 119
Size: 5117
Bsp1286I G_dGCh'C
Cuts at: 161 457 532 640 958 985 998 1630 1831
Size: 296 75 108 318 27 13 632 201
Cuts at: 1831 1844 1986 2131 2742 2835 2909 3099 3161
Size: 13 142 145 611 93 74 190 62
Cuts at: 3161 3891 161
Size: 730 1387
Fragments arranged by size:
1387 730 632 611 318 296 201 190
145 142 108 93 75 74 62 27
13 13
BspEI T'CCGG_A
Cuts at: 1185 4337 1185
Size: 3152 1965
BspHI T'CATG_A
Cuts at: 4293 4293
Size: 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 590 1371 1418 2167 2381 2683 3064 590
Size: 781 47 749 214 302 381 2643
Fragments arranged by size:
2643 781 749 381 302 214 47
BsrBI CCG'CTC
Cuts at: 50 3409 3463 4947 5112 50
Size: 3359 54 1484 165 55
Fragments arranged by size:
3359 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1203 1294 1956 3029 1203
Size: 91 662 1073 3291
Fragments arranged by size:
3291 1073 662 91
BsrFI r'CCGG_y
Cuts at: 259 3115 3296 3505 259
Size: 2856 181 209 1871
Fragments arranged by size:
2856 1871 209 181
BsrGI T'GTAC_A
Cuts at: 362 362
Size: 5117
BssHII G'CGCG_C
Cuts at: 234 234
Size: 5117
BssSI C'ACGA_G
Cuts at: 697 3388 3746 697
Size: 2691 358 2068
Fragments arranged by size:
2691 2068 358
BstAPI GCAn_nnn'nTGC
Cuts at: 339 921 1296 1634 2494 339
Size: 582 375 338 860 2962
Fragments arranged by size:
2962 860 582 375 338
BstBI TT'CG_AA
Cuts at: 3478 3478
Size: 5117
BstYI r'GATC_y
Cuts at: 1 311 404 497 614 926 2142 2609 2632
Size: 310 93 93 117 312 1216 467 23
Cuts at: 2632 2967 3213 4225 4311 4323 4669 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1216 1012 467 449 346 335 312 310
246 117 93 93 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1348 1348
Size: 5117
BtgI C'CryG_G
Cuts at: 186 219 523 1436 186
Size: 33 304 913 3867
Fragments arranged by size:
3867 913 304 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 497 3067 3248 3411 497
Size: 2570 181 163 2203
Fragments arranged by size:
2570 2203 181 163
BtsI GCAGTG_nn'
Cuts at: 689 816 1449 2360 689
Size: 127 633 911 3446
Fragments arranged by size:
3446 911 633 127
DraI TTT'AAA
Cuts at: 2116 2116
Size: 5117
DraIII CAC_nnn'GTG
Cuts at: 606 1764 606
Size: 1158 3959
DrdI GACnn_nn'nnGTC
Cuts at: 1021 1252 2823 3681 1021
Size: 231 1571 858 2457
Fragments arranged by size:
2457 1571 858 231
EaeI y'GGCC_r
Cuts at: 189 413 2702 2876 3267 3294 4639 189
Size: 224 2289 174 391 27 1345 667
Fragments arranged by size:
2289 1345 667 391 224 174 27
EagI C'GGCC_G
Cuts at: 413 2702 413
Size: 2289 2828
EarI CTCTTCn'nnn_
Cuts at: 1426 1468 1819 3140 3350 1426
Size: 42 351 1321 210 3193
Fragments arranged by size:
3193 1321 351 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3325 3635 3781 4927 3325
Size: 310 146 1146 3515
Fragments arranged by size:
3515 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1829 2129 1829
Size: 300 4817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 129 1449 1939 2942 3374 4121 4389 129
Size: 1320 490 1003 432 747 268 857
Fragments arranged by size:
1320 1003 857 747 490 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1955 2256 1955
Size: 301 4816
EcoRI G'AATT_C
Cuts at: 2121 2121
Size: 5117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3136 3136
Size: 5117
FspI TGC'GCA
Cuts at: 214 844 2898 214
Size: 630 2054 2433
Fragments arranged by size:
2433 2054 630
FspAI rTGC'GCAy
Cuts at: 214 844 214
Size: 630 4487
HaeII r_GCGC'y
Cuts at: 666 771 789 1558 1728 1815 2551 2799 3821
Size: 105 18 769 170 87 736 248 1022
Cuts at: 3821 4800 666
Size: 979 983
Fragments arranged by size:
1022 983 979 769 736 248 170 105
87 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 556 588 2959 2989 2991 3021 301
Size: 32 223 32 2371 30 2 30 2397
Fragments arranged by size:
2397 2371 223 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1275 1532 2156 1275
Size: 257 624 4236
Fragments arranged by size:
4236 624 257
HindIII A'AGCT_T
Cuts at: 1008 1008
Size: 5117
KasI G'GCGC_C
Cuts at: 2547 2795 2547
Size: 248 4869
KpnI G_GTAC'C
Cuts at: 2137 2137
Size: 5117
MluI A'CGCG_T
Cuts at: 632 4550 632
Size: 3918 1199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 371 1268 3788 3972 371
Size: 897 2520 184 1516
Fragments arranged by size:
2520 1516 897 184
MscI TGG'CCA
Cuts at: 191 2878 191
Size: 2687 2430
MslI CAynn'nnrTG
Cuts at: 197 1691 1777 1791 2092 3106 3233 197
Size: 1494 86 14 301 1014 127 2081
Fragments arranged by size:
2081 1494 1014 301 127 86 14
MspA1I CmG'CkG
Cuts at: 245 368 525 656 677 746 1138 1396 1438
Size: 123 157 131 21 69 392 258 42
Cuts at: 1438 1548 2228 2542 2902 3915 4160 245
Size: 110 680 314 360 1013 245 1202
Fragments arranged by size:
1202 1013 680 392 360 314 258 245
157 131 123 110 69 42 21
NaeI GCC'GGC
Cuts at: 261 3298 261
Size: 3037 2080
NarI GG'CG_CC
Cuts at: 2548 2796 2548
Size: 248 4869
NgoMIV G'CCGG_C
Cuts at: 259 3296 259
Size: 3037 2080
NsiI A_TGCA'T
Cuts at: 2099 2099
Size: 5117
NspI r_CATG'y
Cuts at: 231 1680 1848 2101 2170 2670 3577 231
Size: 1449 168 253 69 500 907 1771
Fragments arranged by size:
1771 1449 907 500 253 168 69
PciI A'CATG_T
Cuts at: 1676 3573 1676
Size: 1897 3220
PflMI CCAn_nnn'nTGG
Cuts at: 732 732
Size: 5117
PfoI T'CCnGG_A
Cuts at: 275 500 929 1916 275
Size: 225 429 987 3476
Fragments arranged by size:
3476 987 429 225
PmeI GTTT'AAAC
Cuts at: 2116 2116
Size: 5117
PmlI CAC'GTG
Cuts at: 1929 1929
Size: 5117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 4256
Size: 32 5085
PpuMI rG'GwC_Cy
Cuts at: 1955 1955
Size: 5117
PstI C_TGCA'G
Cuts at: 404 431 587 1128 1968 2164 2849 404
Size: 27 156 541 840 196 685 2672
Fragments arranged by size:
2672 840 685 541 196 156 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5117
PvuII CAG'CTG
Cuts at: 746 1396 1548 2542 2902 746
Size: 650 152 994 360 2961
Fragments arranged by size:
2961 994 650 360 152
RsrII CG'GwC_CG
Cuts at: 3312 3312
Size: 5117
SacI G_AGCT'C
Cuts at: 1831 2131 1831
Size: 300 4817
SacII CC_GC'GG
Cuts at: 526 1439 526
Size: 913 4204
SalI G'TCGA_C
Cuts at: 1530 2154 1530
Size: 624 4493
SanDI GG'GwC_CC
Cuts at: 1955 1955
Size: 5117
SapI GCTCTTCn'nnn_
Cuts at: 1819 3140 3350 1819
Size: 1321 210 3586
Fragments arranged by size:
3586 1321 210
SbfI CC_TGCA'GG
Cuts at: 1968 2164 1968
Size: 196 4921
ScaI AGT'ACT
Cuts at: 4634 4634
Size: 5117
SfcI C'TryA_G
Cuts at: 33 297 400 427 583 1124 1964 2160 2845
Size: 264 103 27 156 541 840 196 685
Cuts at: 2845 3838 4029 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 840 685 541 264 196 191
156 103 27
SfoI GGC'GCC
Cuts at: 2549 2797 2549
Size: 248 4869
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5117
SmaI CCC'GGG
Cuts at: 494 2139 494
Size: 1645 3472
SmlI C'TyrA_G
Cuts at: 160 2272 3187 3679 3941 160
Size: 2112 915 492 262 1336
Fragments arranged by size:
2112 1336 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5117
SphI G_CATG'C
Cuts at: 231 2170 2670 231
Size: 1939 500 2678
Fragments arranged by size:
2678 1939 500
StyI C'CwwG_G
Cuts at: 2251 2605 2251
Size: 354 4763
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 608 1123 1260 1834 2700 2833 3292 3501
Size: 481 515 137 574 866 133 459 209
Cuts at: 3501 127
Size: 1743
Fragments arranged by size:
1743 866 574 515 481 459 209 137
133
TatI w'GTAC_w
Cuts at: 362 4632 362
Size: 4270 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4634 4666 4634
Size: 32 5085
Tth111I GACn'n_nGTC
Cuts at: 1512 2914 1512
Size: 1402 3715
XbaI T'CTAG_A
Cuts at: 2148 2148
Size: 5117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2612 2612
Size: 5117
XhoI C'TCGA_G
Cuts at: 160 160
Size: 5117
XmaI C'CCGG_G
Cuts at: 492 2137 492
Size: 1645 3472
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BmtI BsiWI BsmBI BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XmnI ZraI