(Circular) (Six-base) MAPSORT of: pF1KB8327.seq Check: 6833 from: 1 to: 3875 pF1KB8327 3875 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 913 913 Size: 3875 Acc65I G'GTAC_C Cuts at: 891 891 Size: 3875 AclI AA'CG_TT Cuts at: 3715 3715 Size: 3875 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1700 2132 2879 3147 1700 Size: 432 747 268 2428 Fragments arranged by size: 2428 747 432 268 AflIII A'CryG_T Cuts at: 1227 2331 3308 1227 Size: 1104 977 1794 Fragments arranged by size: 1794 1104 977 AgeI A'CCGG_T Cuts at: 2263 2263 Size: 3875 AhdI GACnn_n'nnGTC Cuts at: 237 237 Size: 3875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 632 632 Size: 3875 AlwNI CAG_nnn'CTG Cuts at: 1180 2747 1180 Size: 1567 2308 ApaI G_GGCC'C Cuts at: 432 432 Size: 3875 ApaLI G'TGCA_C Cuts at: 2645 2645 Size: 3875 ApoI r'AATT_y Cuts at: 879 3228 3484 879 Size: 2349 256 1270 Fragments arranged by size: 2349 1270 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3875 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3875 AvaI C'yCGr_G Cuts at: 242 284 369 895 242 Size: 42 85 526 3222 Fragments arranged by size: 3222 526 85 42 BamHI G'GATC_C Cuts at: 900 900 Size: 3875 BanI G'GyrC_C Cuts at: 891 1305 1553 1588 891 Size: 414 248 35 3178 Fragments arranged by size: 3178 414 248 35 BanII G_rGCy'C Cuts at: 171 432 889 1919 171 Size: 261 457 1030 2127 Fragments arranged by size: 2127 1030 457 261 BbeI G_GCGC'C Cuts at: 1309 1557 1309 Size: 248 3627 BbvCI CC'TCA_GC Cuts at: 757 809 757 Size: 52 3823 BciVI GTATCCnnnnn_n' Cuts at: 1773 2534 3875 1773 Size: 761 1341 1773 Fragments arranged by size: 1773 1341 761 BclI T'GATC_A Cuts at: 299 1395 299 Size: 1096 2779 BglI GCCn_nnn'nGGC Cuts at: 155 155 Size: 3875 BglII A'GATC_T Cuts at: 650 1390 3427 650 Size: 740 2037 1098 Fragments arranged by size: 2037 1098 740 BlpI GC'TnA_GC Cuts at: 145 987 145 Size: 842 3033 Bme1580I G_kGCm'C Cuts at: 432 1500 1593 2649 432 Size: 1068 93 1056 1658 Fragments arranged by size: 1658 1068 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 259 1087 1168 1503 3647 259 Size: 828 81 335 2144 487 Fragments arranged by size: 2144 828 487 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 3875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 185 584 185 Size: 399 3476 Bpu10I CC'TnA_GC Cuts at: 757 809 757 Size: 52 3823 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1051 1930 2422 2720 1051 Size: 879 492 298 2206 Fragments arranged by size: 2206 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 484 3579 484 Size: 3095 780 BsaAI yAC'GTr Cuts at: 218 1228 218 Size: 1010 2865 BsaBI GATnn'nnATC Cuts at: 6 1389 3426 6 Size: 1383 2037 455 Fragments arranged by size: 2037 1383 455 BsaHI Gr'CG_yC Cuts at: 1306 1554 2256 3752 1306 Size: 248 702 1496 1429 Fragments arranged by size: 1496 1429 702 248 BsaWI w'CCGG_w Cuts at: 1064 1585 2263 2537 2684 3095 1064 Size: 521 678 274 147 411 1844 Fragments arranged by size: 1844 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 379 739 742 781 379 Size: 360 3 39 3473 Fragments arranged by size: 3473 360 39 3 BseYI C'CCAG_C Cuts at: 225 293 309 1300 2058 2635 3131 225 Size: 68 16 991 758 577 496 969 Fragments arranged by size: 991 969 758 577 496 68 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 192 874 192 Size: 682 3193 BsiEI CG_ry'CG Cuts at: 104 1417 1463 2671 104 Size: 1313 46 1208 1308 Fragments arranged by size: 1313 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 889 1667 1857 2649 889 Size: 778 190 792 2115 Fragments arranged by size: 2115 792 778 190 Bsp1286I G_dGCh'C Cuts at: 171 432 889 1500 1593 1667 1857 1919 2649 Size: 261 457 611 93 74 190 62 730 Cuts at: 2649 171 Size: 1397 Fragments arranged by size: 1397 730 611 457 261 190 93 74 62 BspEI T'CCGG_A Cuts at: 3095 3095 Size: 3875 BspHI T'CATG_A Cuts at: 3051 3051 Size: 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 230 925 1139 1441 1822 230 Size: 695 214 302 381 2283 Fragments arranged by size: 2283 695 381 302 214 BsrBI CCG'CTC Cuts at: 50 2167 2221 3705 3870 50 Size: 2117 54 1484 165 55 Fragments arranged by size: 2117 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1787 1787 Size: 3875 BsrFI r'CCGG_y Cuts at: 1873 2054 2263 1873 Size: 181 209 3485 Fragments arranged by size: 3485 209 181 BssSI C'ACGA_G Cuts at: 2146 2504 2146 Size: 358 3517 BstAPI GCAn_nnn'nTGC Cuts at: 1252 1252 Size: 3875 BstBI TT'CG_AA Cuts at: 2236 2236 Size: 3875 BstYI r'GATC_y Cuts at: 1 650 900 1367 1390 1725 1971 2983 3069 Size: 649 250 467 23 335 246 1012 86 Cuts at: 3069 3081 3427 1 Size: 12 346 449 Fragments arranged by size: 1012 649 467 449 346 335 250 246 86 23 12 BtgI C'CryG_G Cuts at: 107 582 699 719 107 Size: 475 117 20 3263 Fragments arranged by size: 3263 475 117 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1825 2006 2169 1825 Size: 181 163 3531 Fragments arranged by size: 3531 181 163 BtsI GCAGTG_nn' Cuts at: 342 863 1118 342 Size: 521 255 3099 Fragments arranged by size: 3099 521 255 DraI TTT'AAA Cuts at: 874 874 Size: 3875 DrdI GACnn_nn'nnGTC Cuts at: 1581 2439 1581 Size: 858 3017 EaeI y'GGCC_r Cuts at: 507 1460 1634 2025 2052 3397 507 Size: 953 174 391 27 1345 985 Fragments arranged by size: 1345 985 953 391 174 27 EagI C'GGCC_G Cuts at: 1460 1460 Size: 3875 EarI CTCTTCn'nnn_ Cuts at: 1898 2108 1898 Size: 210 3665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 609 2083 2393 2539 3685 127 Size: 482 1474 310 146 1146 317 Fragments arranged by size: 1474 1146 482 317 310 146 EcoICRI GAG'CTC Cuts at: 887 887 Size: 3875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 185 584 1700 2132 2879 3147 185 Size: 399 1116 432 747 268 913 Fragments arranged by size: 1116 913 747 432 399 268 EcoO109I rG'GnC_Cy Cuts at: 428 1014 428 Size: 586 3289 EcoRI G'AATT_C Cuts at: 879 879 Size: 3875 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1894 1894 Size: 3875 FspI TGC'GCA Cuts at: 1656 1656 Size: 3875 HaeII r_GCGC'y Cuts at: 283 480 1309 1557 2579 3558 283 Size: 197 829 248 1022 979 600 Fragments arranged by size: 1022 979 829 600 248 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 158 190 223 255 445 449 477 481 1717 Size: 32 33 32 190 4 28 4 1236 Cuts at: 1717 1747 1749 1779 158 Size: 30 2 30 2254 Fragments arranged by size: 2254 1236 190 33 32 32 30 30 28 4 4 2 HincII GTy'rAC Cuts at: 914 914 Size: 3875 KasI G'GCGC_C Cuts at: 1305 1553 1305 Size: 248 3627 KpnI G_GTAC'C Cuts at: 895 895 Size: 3875 MluI A'CGCG_T Cuts at: 3308 3308 Size: 3875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 467 2546 2730 467 Size: 2079 184 1612 Fragments arranged by size: 2079 1612 184 MscI TGG'CCA Cuts at: 509 1636 509 Size: 1127 2748 MslI CAynn'nnrTG Cuts at: 1864 1991 1864 Size: 127 3748 MspA1I CmG'CkG Cuts at: 144 297 761 986 1300 1660 2673 2918 144 Size: 153 464 225 314 360 1013 245 1101 Fragments arranged by size: 1101 1013 464 360 314 245 225 153 NaeI GCC'GGC Cuts at: 2056 2056 Size: 3875 NarI GG'CG_CC Cuts at: 1306 1554 1306 Size: 248 3627 NcoI C'CATG_G Cuts at: 107 699 107 Size: 592 3283 NgoMIV G'CCGG_C Cuts at: 2054 2054 Size: 3875 NspI r_CATG'y Cuts at: 843 928 1428 2335 843 Size: 85 500 907 2383 Fragments arranged by size: 2383 907 500 85 PciI A'CATG_T Cuts at: 2331 2331 Size: 3875 PflMI CCAn_nnn'nTGG Cuts at: 552 552 Size: 3875 PfoI T'CCnGG_A Cuts at: 269 663 269 Size: 394 3481 PmeI GTTT'AAAC Cuts at: 874 874 Size: 3875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3014 3046 3014 Size: 32 3843 PspOMI G'GGCC_C Cuts at: 428 428 Size: 3875 PstI C_TGCA'G Cuts at: 922 1607 922 Size: 685 3190 PvuI CG_AT'CG Cuts at: 104 104 Size: 3875 PvuII CAG'CTG Cuts at: 144 297 761 1300 1660 144 Size: 153 464 539 360 2359 Fragments arranged by size: 2359 539 464 360 153 RsrII CG'GwC_CG Cuts at: 2070 2070 Size: 3875 SacI G_AGCT'C Cuts at: 889 889 Size: 3875 SalI G'TCGA_C Cuts at: 912 912 Size: 3875 SapI GCTCTTCn'nnn_ Cuts at: 1898 2108 1898 Size: 210 3665 SbfI CC_TGCA'GG Cuts at: 922 922 Size: 3875 ScaI AGT'ACT Cuts at: 3392 3392 Size: 3875 SfcI C'TryA_G Cuts at: 33 918 1603 2596 2787 33 Size: 885 685 993 191 1121 Fragments arranged by size: 1121 993 885 685 191 SfoI GGC'GCC Cuts at: 1307 1555 1307 Size: 248 3627 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3875 SmaI CCC'GGG Cuts at: 244 897 244 Size: 653 3222 SmlI C'TyrA_G Cuts at: 1030 1945 2437 2699 1030 Size: 915 492 262 2206 Fragments arranged by size: 2206 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3875 SphI G_CATG'C Cuts at: 928 1428 928 Size: 500 3375 StyI C'CwwG_G Cuts at: 107 699 1009 1363 107 Size: 592 310 354 2619 Fragments arranged by size: 2619 592 354 310 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 217 575 627 876 1458 1591 2050 2259 217 Size: 358 52 249 582 133 459 209 1833 Fragments arranged by size: 1833 582 459 358 249 209 133 52 TatI w'GTAC_w Cuts at: 120 3390 120 Size: 3270 605 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3392 3424 3392 Size: 32 3843 Tth111I GACn'n_nGTC Cuts at: 265 1672 265 Size: 1407 2468 XbaI T'CTAG_A Cuts at: 906 906 Size: 3875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1370 1370 Size: 3875 XmaI C'CCGG_G Cuts at: 242 895 242 Size: 653 3222 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AloI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI