Restriction map for
pF1KB8328
(Circular) (Six-base) MAPSORT of: pF1KB8328.seq Check: 4505 from: 1 to: 3632
pF1KB8328 3632 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 317 317
Size: 3632
AccI GT'mk_AC
Cuts at: 670 670
Size: 3632
Acc65I G'GTAC_C
Cuts at: 648 648
Size: 3632
AclI AA'CG_TT
Cuts at: 3472 3472
Size: 3632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 1457 1889 2636 2904 585
Size: 872 432 747 268 1313
Fragments arranged by size:
1313 872 747 432 268
AflIII A'CryG_T
Cuts at: 984 2088 3065 984
Size: 1104 977 1551
Fragments arranged by size:
1551 1104 977
AgeI A'CCGG_T
Cuts at: 2020 2020
Size: 3632
AhdI GACnn_n'nnGTC
Cuts at: 435 435
Size: 3632
AleI CACnn'nnGTG
Cuts at: 556 556
Size: 3632
AlwNI CAG_nnn'CTG
Cuts at: 937 2504 937
Size: 1567 2065
ApaI G_GGCC'C
Cuts at: 542 542
Size: 3632
ApaLI G'TGCA_C
Cuts at: 2402 2402
Size: 3632
ApoI r'AATT_y
Cuts at: 636 2985 3241 636
Size: 2349 256 1027
Fragments arranged by size:
2349 1027 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
AvaI C'yCGr_G
Cuts at: 341 652 341
Size: 311 3321
BamHI G'GATC_C
Cuts at: 657 657
Size: 3632
BanI G'GyrC_C
Cuts at: 130 406 648 1062 1310 1345 130
Size: 276 242 414 248 35 2417
Fragments arranged by size:
2417 414 276 248 242 35
BanII G_rGCy'C
Cuts at: 194 542 646 1676 194
Size: 348 104 1030 2150
Fragments arranged by size:
2150 1030 348 104
BbeI G_GCGC'C
Cuts at: 134 1066 1314 134
Size: 932 248 2452
Fragments arranged by size:
2452 932 248
BbsI GAAGACnn'nnnn_
Cuts at: 243 243
Size: 3632
BciVI GTATCCnnnnn_n'
Cuts at: 1530 2291 3632 1530
Size: 761 1341 1530
Fragments arranged by size:
1530 1341 761
BclI T'GATC_A
Cuts at: 1152 1152
Size: 3632
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3632
BglII A'GATC_T
Cuts at: 263 1147 3184 263
Size: 884 2037 711
Fragments arranged by size:
2037 884 711
BlpI GC'TnA_GC
Cuts at: 171 744 171
Size: 573 3059
Bme1580I G_kGCm'C
Cuts at: 529 542 1257 1350 2406 529
Size: 13 715 93 1056 1755
Fragments arranged by size:
1755 1056 715 93 13
BmrI ACTGGGnnnn_n'
Cuts at: 844 925 1260 3404 844
Size: 81 335 2144 1072
Fragments arranged by size:
2144 1072 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 396 208
Size: 188 3444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 1687 2179 2477 808
Size: 879 492 298 1963
Fragments arranged by size:
1963 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 3336 238
Size: 3098 534
BsaAI yAC'GTr
Cuts at: 985 985
Size: 3632
BsaBI GATnn'nnATC
Cuts at: 6 1146 3183 6
Size: 1140 2037 455
Fragments arranged by size:
2037 1140 455
BsaHI Gr'CG_yC
Cuts at: 131 314 1063 1311 2013 3509 131
Size: 183 749 248 702 1496 254
Fragments arranged by size:
1496 749 702 254 248 183
BsaWI w'CCGG_w
Cuts at: 821 1342 2020 2294 2441 2852 821
Size: 521 678 274 147 411 1601
Fragments arranged by size:
1601 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 448 439
Size: 9 3623
BseYI C'CCAG_C
Cuts at: 1057 1815 2392 2888 1057
Size: 758 577 496 1801
Fragments arranged by size:
1801 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 575 1174 1220 2428 104
Size: 471 599 46 1208 1308
Fragments arranged by size:
1308 1208 599 471 46
BsiHKAI G_wGCw'C
Cuts at: 646 1424 1614 2406 646
Size: 778 190 792 1872
Fragments arranged by size:
1872 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 194 529 542 646 1257 1350 1424 1614 1676
Size: 335 13 104 611 93 74 190 62
Cuts at: 1676 2406 194
Size: 730 1420
Fragments arranged by size:
1420 730 611 335 190 104 93 74
62 13
BspEI T'CCGG_A
Cuts at: 2852 2852
Size: 3632
BspHI T'CATG_A
Cuts at: 2808 2808
Size: 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 682 896 1198 1579 682
Size: 214 302 381 2735
Fragments arranged by size:
2735 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1924 1978 3462 3627 50
Size: 1874 54 1484 165 55
Fragments arranged by size:
1874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1544 1544
Size: 3632
BsrFI r'CCGG_y
Cuts at: 1630 1811 2020 1630
Size: 181 209 3242
Fragments arranged by size:
3242 209 181
BssSI C'ACGA_G
Cuts at: 1903 2261 1903
Size: 358 3274
BstAPI GCAn_nnn'nTGC
Cuts at: 1009 1009
Size: 3632
BstBI TT'CG_AA
Cuts at: 1993 1993
Size: 3632
BstYI r'GATC_y
Cuts at: 1 263 657 1124 1147 1482 1728 2740 2826
Size: 262 394 467 23 335 246 1012 86
Cuts at: 2826 2838 3184 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 394 346 335 262 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 451 451
Size: 3632
BtgI C'CryG_G
Cuts at: 107 107
Size: 3632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 130 1582 1763 1926 130
Size: 1452 181 163 1836
Fragments arranged by size:
1836 1452 181 163
BtsI GCAGTG_nn'
Cuts at: 875 875
Size: 3632
DraI TTT'AAA
Cuts at: 631 631
Size: 3632
DrdI GACnn_nn'nnGTC
Cuts at: 1338 2196 1338
Size: 858 2774
EaeI y'GGCC_r
Cuts at: 1217 1391 1782 1809 3154 1217
Size: 174 391 27 1345 1695
Fragments arranged by size:
1695 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1217 1217
Size: 3632
EarI CTCTTCn'nnn_
Cuts at: 604 1655 1865 604
Size: 1051 210 2371
Fragments arranged by size:
2371 1051 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1840 2150 2296 3442 1840
Size: 310 146 1146 2030
Fragments arranged by size:
2030 1146 310 146
EcoICRI GAG'CTC
Cuts at: 644 644
Size: 3632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 396 585 1457 1889 2636 2904 208
Size: 188 189 872 432 747 268 936
Fragments arranged by size:
936 872 747 432 268 189 188
EcoNI CCTnn'n_nnAGG
Cuts at: 454 454
Size: 3632
EcoO109I rG'GnC_Cy
Cuts at: 539 771 539
Size: 232 3400
EcoRI G'AATT_C
Cuts at: 636 636
Size: 3632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1651 1651
Size: 3632
FspI TGC'GCA
Cuts at: 1413 1413
Size: 3632
HaeII r_GCGC'y
Cuts at: 134 142 398 1066 1314 2336 3315 134
Size: 8 256 668 248 1022 979 451
Fragments arranged by size:
1022 979 668 451 256 248 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 108 140 421 453 1474 1504 1506 1536 108
Size: 32 281 32 1021 30 2 30 2204
Fragments arranged by size:
2204 1021 281 32 32 30 30 2
HincII GTy'rAC
Cuts at: 671 671
Size: 3632
KasI G'GCGC_C
Cuts at: 130 1062 1310 130
Size: 932 248 2452
Fragments arranged by size:
2452 932 248
KpnI G_GTAC'C
Cuts at: 652 652
Size: 3632
MluI A'CGCG_T
Cuts at: 3065 3065
Size: 3632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2303 2487 2303
Size: 184 3448
MscI TGG'CCA
Cuts at: 1393 1393
Size: 3632
MslI CAynn'nnrTG
Cuts at: 556 1621 1748 556
Size: 1065 127 2440
Fragments arranged by size:
2440 1065 127
MspA1I CmG'CkG
Cuts at: 167 743 1057 1417 2430 2675 167
Size: 576 314 360 1013 245 1124
Fragments arranged by size:
1124 1013 576 360 314 245
NaeI GCC'GGC
Cuts at: 1813 1813
Size: 3632
NarI GG'CG_CC
Cuts at: 131 1063 1311 131
Size: 932 248 2452
Fragments arranged by size:
2452 932 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3632
NgoMIV G'CCGG_C
Cuts at: 1811 1811
Size: 3632
NspI r_CATG'y
Cuts at: 278 685 1185 2092 278
Size: 407 500 907 1818
Fragments arranged by size:
1818 907 500 407
PasI CC'CwG_GG
Cuts at: 542 542
Size: 3632
PciI A'CATG_T
Cuts at: 2088 2088
Size: 3632
PflMI CCAn_nnn'nTGG
Cuts at: 297 354 297
Size: 57 3575
PfoI T'CCnGG_A
Cuts at: 373 373
Size: 3632
PmeI GTTT'AAAC
Cuts at: 631 631
Size: 3632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2771 2803 2771
Size: 32 3600
PspOMI G'GGCC_C
Cuts at: 538 538
Size: 3632
PspXI vC'TCGA_Gb
Cuts at: 341 341
Size: 3632
PstI C_TGCA'G
Cuts at: 679 1364 679
Size: 685 2947
PvuI CG_AT'CG
Cuts at: 104 575 104
Size: 471 3161
PvuII CAG'CTG
Cuts at: 1057 1417 1057
Size: 360 3272
RsrII CG'GwC_CG
Cuts at: 1827 1827
Size: 3632
SacI G_AGCT'C
Cuts at: 646 646
Size: 3632
SalI G'TCGA_C
Cuts at: 669 669
Size: 3632
SapI GCTCTTCn'nnn_
Cuts at: 604 1655 1865 604
Size: 1051 210 2371
Fragments arranged by size:
2371 1051 210
SbfI CC_TGCA'GG
Cuts at: 679 679
Size: 3632
ScaI AGT'ACT
Cuts at: 3149 3149
Size: 3632
SfcI C'TryA_G
Cuts at: 33 441 675 1360 2353 2544 33
Size: 408 234 685 993 191 1121
Fragments arranged by size:
1121 993 685 408 234 191
SfoI GGC'GCC
Cuts at: 132 1064 1312 132
Size: 932 248 2452
Fragments arranged by size:
2452 932 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3632
SmaI CCC'GGG
Cuts at: 654 654
Size: 3632
SmlI C'TyrA_G
Cuts at: 341 787 1702 2194 2456 341
Size: 446 915 492 262 1517
Fragments arranged by size:
1517 915 492 446 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3632
SphI G_CATG'C
Cuts at: 685 1185 685
Size: 500 3132
StuI AGG'CCT
Cuts at: 181 181
Size: 3632
StyI C'CwwG_G
Cuts at: 107 766 1120 107
Size: 659 354 2619
Fragments arranged by size:
2619 659 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 534 1215 1348 1807 2016 534
Size: 681 133 459 209 2150
Fragments arranged by size:
2150 681 459 209 133
TatI w'GTAC_w
Cuts at: 3147 3147
Size: 3632
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3600
Tth111I GACn'n_nGTC
Cuts at: 229 1429 229
Size: 1200 2432
XbaI T'CTAG_A
Cuts at: 663 663
Size: 3632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 558 1127 114
Size: 444 569 2619
Fragments arranged by size:
2619 569 444
XhoI C'TCGA_G
Cuts at: 341 341
Size: 3632
XmaI C'CCGG_G
Cuts at: 652 652
Size: 3632
ZraI GAC'GTC
Cuts at: 315 315
Size: 3632
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI