Restriction map for
pF1KB8337
(Circular) (Six-base) MAPSORT of: pF1KB8337.seq Check: 478 from: 1 to: 4730
pF1KB8337 4730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1768 1768
Size: 4730
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 887 891 1143 1563 2555 2987 3734 4002
Size: 164 4 252 420 992 432 747 268
Cuts at: 4002 723
Size: 1451
Fragments arranged by size:
1451 992 747 432 420 268 252 164
4
AflIII A'CryG_T
Cuts at: 1679 2082 3186 4163 1679
Size: 403 1104 977 2246
Fragments arranged by size:
2246 1104 977 403
AgeI A'CCGG_T
Cuts at: 965 3118 965
Size: 2153 2577
AleI CACnn'nnGTG
Cuts at: 632 632
Size: 4730
AlwNI CAG_nnn'CTG
Cuts at: 809 1530 2035 3602 809
Size: 721 505 1567 1937
Fragments arranged by size:
1937 1567 721 505
ApaI G_GGCC'C
Cuts at: 205 205
Size: 4730
ApaLI G'TGCA_C
Cuts at: 3500 3500
Size: 4730
ApoI r'AATT_y
Cuts at: 773 1277 1734 4083 4339 773
Size: 504 457 2349 256 1164
Fragments arranged by size:
2349 1164 504 457 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 179 1750 179
Size: 1571 3159
BamHI G'GATC_C
Cuts at: 1613 1755 1613
Size: 142 4588
BanI G'GyrC_C
Cuts at: 349 1746 2160 2408 2443 349
Size: 1397 414 248 35 2636
Fragments arranged by size:
2636 1397 414 248 35
BanII G_rGCy'C
Cuts at: 205 329 1744 2774 205
Size: 124 1415 1030 2161
Fragments arranged by size:
2161 1415 1030 124
BbeI G_GCGC'C
Cuts at: 2164 2412 2164
Size: 248 4482
BbsI GAAGACnn'nnnn_
Cuts at: 991 991
Size: 4730
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3389 4730 2628
Size: 761 1341 2628
Fragments arranged by size:
2628 1341 761
BclI T'GATC_A
Cuts at: 615 2250 615
Size: 1635 3095
BglI GCCn_nnn'nGGC
Cuts at: 112 200 112
Size: 88 4642
BglII A'GATC_T
Cuts at: 2245 4282 2245
Size: 2037 2693
BlpI GC'TnA_GC
Cuts at: 1842 1842
Size: 4730
Bme1580I G_kGCm'C
Cuts at: 205 352 2355 2448 3504 205
Size: 147 2003 93 1056 1431
Fragments arranged by size:
2003 1431 1056 147 93
BmgBI CAC'GTC
Cuts at: 162 231 162
Size: 69 4661
BmrI ACTGGGnnnn_n'
Cuts at: 274 791 1043 1942 2023 2358 4502 274
Size: 517 252 899 81 335 2144 502
Fragments arranged by size:
2144 899 517 502 335 252 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1734 3097 1734
Size: 1363 3367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 314 1307 1391 259
Size: 55 993 84 3598
Fragments arranged by size:
3598 993 84 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1906 2785 3277 3575 1906
Size: 879 492 298 3061
Fragments arranged by size:
3061 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 235 615 4434 235
Size: 380 3819 531
Fragments arranged by size:
3819 531 380
BsaAI yAC'GTr
Cuts at: 1592 2083 1592
Size: 491 4239
BsaBI GATnn'nnATC
Cuts at: 6 2244 4281 6
Size: 2238 2037 455
Fragments arranged by size:
2238 2037 455
BsaHI Gr'CG_yC
Cuts at: 2161 2409 3111 4607 2161
Size: 248 702 1496 2284
Fragments arranged by size:
2284 1496 702 248
BsaWI w'CCGG_w
Cuts at: 965 1117 1201 1607 1919 2440 3118 3392 3539
Size: 152 84 406 312 521 678 274 147
Cuts at: 3539 3950 965
Size: 411 1745
Fragments arranged by size:
1745 678 521 411 406 312 274 152
147 84
BseYI C'CCAG_C
Cuts at: 384 2155 2913 3490 3986 384
Size: 1771 758 577 496 1128
Fragments arranged by size:
1771 1128 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1494 1516 1662 1494
Size: 22 146 4562
Fragments arranged by size:
4562 146 22
BsiEI CG_ry'CG
Cuts at: 104 2272 2318 3526 104
Size: 2168 46 1208 1308
Fragments arranged by size:
2168 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 368 1591 1744 2522 2712 3504 368
Size: 1223 153 778 190 792 1594
Fragments arranged by size:
1594 1223 792 778 190 153
BsmI GAATG_Cn'
Cuts at: 1393 1393
Size: 4730
Bsp1286I G_dGCh'C
Cuts at: 205 329 352 368 1591 1744 2355 2448 2522
Size: 124 23 16 1223 153 611 93 74
Cuts at: 2522 2712 2774 3504 205
Size: 190 62 730 1431
Fragments arranged by size:
1431 1223 730 611 190 153 124 93
74 62 23 16
BspEI T'CCGG_A
Cuts at: 3950 3950
Size: 4730
BspHI T'CATG_A
Cuts at: 1010 3906 1010
Size: 2896 1834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 1780 1994 2296 2677 285
Size: 1495 214 302 381 2338
Fragments arranged by size:
2338 1495 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3022 3076 4560 4725 50
Size: 2972 54 1484 165 55
Fragments arranged by size:
2972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1398 2642 1398
Size: 1244 3486
BsrFI r'CCGG_y
Cuts at: 965 2728 2909 3118 965
Size: 1763 181 209 2577
Fragments arranged by size:
2577 1763 209 181
BsrGI T'GTAC_A
Cuts at: 690 690
Size: 4730
BssSI C'ACGA_G
Cuts at: 1345 3001 3359 1345
Size: 1656 358 2716
Fragments arranged by size:
2716 1656 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1565 2107 1565
Size: 542 4188
BstBI TT'CG_AA
Cuts at: 3091 3091
Size: 4730
BstEII G'GTnAC_C
Cuts at: 147 147
Size: 4730
BstXI CCAn_nnnn'nTGG
Cuts at: 1347 1347
Size: 4730
BstYI r'GATC_y
Cuts at: 1 447 457 1613 1755 2222 2245 2580 2826
Size: 446 10 1156 142 467 23 335 246
Cuts at: 2826 3838 3924 3936 4282 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1156 1012 467 449 446 346 335 246
142 86 23 12 10
Bsu36I CC'TnA_GG
Cuts at: 589 589
Size: 4730
BtgI C'CryG_G
Cuts at: 107 1536 1721 107
Size: 1429 185 3116
Fragments arranged by size:
3116 1429 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2680 2861 3024 2680
Size: 181 163 4386
Fragments arranged by size:
4386 181 163
BtsI GCAGTG_nn'
Cuts at: 1973 1973
Size: 4730
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1212 1247 1380 1415 1212
Size: 35 133 35 4527
Fragments arranged by size:
4527 133 35 35
DraI TTT'AAA
Cuts at: 1729 1729
Size: 4730
DrdI GACnn_nn'nnGTC
Cuts at: 2436 3294 2436
Size: 858 3872
EaeI y'GGCC_r
Cuts at: 2315 2489 2880 2907 4252 2315
Size: 174 391 27 1345 2793
Fragments arranged by size:
2793 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2315 2315
Size: 4730
EarI CTCTTCn'nnn_
Cuts at: 863 1092 2753 2963 863
Size: 229 1661 210 2630
Fragments arranged by size:
2630 1661 229 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2938 3248 3394 4540 2938
Size: 310 146 1146 3128
Fragments arranged by size:
3128 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1742 1742
Size: 4730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 314 723 887 891 1143 1307 1391 1563
Size: 55 409 164 4 252 164 84 172
Cuts at: 1563 2555 2987 3734 4002 259
Size: 992 432 747 268 987
Fragments arranged by size:
992 987 747 432 409 268 252 172
164 164 84 55 4
EcoNI CCTnn'n_nnAGG
Cuts at: 1708 1708
Size: 4730
EcoO109I rG'GnC_Cy
Cuts at: 202 216 274 283 333 532 1869 202
Size: 14 58 9 50 199 1337 3063
Fragments arranged by size:
3063 1337 199 58 50 14 9
EcoRI G'AATT_C
Cuts at: 1277 1734 1277
Size: 457 4273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1593 2749 1593
Size: 1156 3574
FspI TGC'GCA
Cuts at: 2511 2511
Size: 4730
HaeII r_GCGC'y
Cuts at: 1082 2164 2412 3434 4413 1082
Size: 1082 248 1022 979 1399
Fragments arranged by size:
1399 1082 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 654 656 686 688 2572 2602 2604 2634 654
Size: 2 30 2 1884 30 2 30 2750
Fragments arranged by size:
2750 1884 30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 378 419 1769 378
Size: 41 1350 3339
Fragments arranged by size:
3339 1350 41
HindIII A'AGCT_T
Cuts at: 1322 1322
Size: 4730
KasI G'GCGC_C
Cuts at: 2160 2408 2160
Size: 248 4482
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 290 1171 1206 1290 1700 3401 3585 290
Size: 881 35 84 410 1701 184 1435
Fragments arranged by size:
1701 1435 881 410 184 84 35
MscI TGG'CCA
Cuts at: 2491 2491
Size: 4730
MslI CAynn'nnrTG
Cuts at: 632 686 1218 2719 2846 632
Size: 54 532 1501 127 2516
Fragments arranged by size:
2516 1501 532 127 54
MspA1I CmG'CkG
Cuts at: 855 1695 1841 2155 2515 3528 3773 855
Size: 840 146 314 360 1013 245 1812
Fragments arranged by size:
1812 1013 840 360 314 245 146
NaeI GCC'GGC
Cuts at: 2911 2911
Size: 4730
NarI GG'CG_CC
Cuts at: 2161 2409 2161
Size: 248 4482
NcoI C'CATG_G
Cuts at: 107 1721 107
Size: 1614 3116
NgoMIV G'CCGG_C
Cuts at: 2909 2909
Size: 4730
NspI r_CATG'y
Cuts at: 1683 1783 2283 3190 1683
Size: 100 500 907 3223
Fragments arranged by size:
3223 907 500 100
PasI CC'CwG_GG
Cuts at: 329 453 536 329
Size: 124 83 4523
Fragments arranged by size:
4523 124 83
PciI A'CATG_T
Cuts at: 1679 3186 1679
Size: 1507 3223
PflMI CCAn_nnn'nTGG
Cuts at: 391 391
Size: 4730
PfoI T'CCnGG_A
Cuts at: 335 1706 335
Size: 1371 3359
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 3869 3901 244
Size: 32 3593 32 1073
Fragments arranged by size:
3593 1073 32 32
PpuMI rG'GwC_Cy
Cuts at: 274 333 274
Size: 59 4671
PspOMI G'GGCC_C
Cuts at: 201 201
Size: 4730
PstI C_TGCA'G
Cuts at: 282 1777 2462 282
Size: 1495 685 2550
Fragments arranged by size:
2550 1495 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 2155 2515 2155
Size: 360 4370
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 1744 1744
Size: 4730
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 2753 2963 2753
Size: 210 4520
SbfI CC_TGCA'GG
Cuts at: 282 1777 282
Size: 1495 3235
ScaI AGT'ACT
Cuts at: 4247 4247
Size: 4730
SfcI C'TryA_G
Cuts at: 33 222 278 1551 1773 2458 3451 3642 33
Size: 189 56 1273 222 685 993 191 1121
Fragments arranged by size:
1273 1121 993 685 222 191 189 56
SfiI GGCCn_nnn'nGGCC
Cuts at: 200 200
Size: 4730
SfoI GGC'GCC
Cuts at: 2162 2410 2162
Size: 248 4482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SmaI CCC'GGG
Cuts at: 181 1752 181
Size: 1571 3159
SmlI C'TyrA_G
Cuts at: 1885 2800 3292 3554 1885
Size: 915 492 262 3061
Fragments arranged by size:
3061 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 1783 2283 1783
Size: 500 4230
StuI AGG'CCT
Cuts at: 1492 1660 1492
Size: 168 4562
StyI C'CwwG_G
Cuts at: 107 1721 1864 2218 107
Size: 1614 143 354 2619
Fragments arranged by size:
2619 1614 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2313 2446 2905 3114 2313
Size: 133 459 209 3929
Fragments arranged by size:
3929 459 209 133
TatI w'GTAC_w
Cuts at: 407 690 4245 407
Size: 283 3555 892
Fragments arranged by size:
3555 892 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1415 1447 1489 1521 4247 4279 1415
Size: 32 42 32 2726 32 1866
Fragments arranged by size:
2726 1866 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1187 2527 1187
Size: 1340 3390
XbaI T'CTAG_A
Cuts at: 1761 1761
Size: 4730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2225 2225
Size: 4730
XmaI C'CCGG_G
Cuts at: 179 1750 179
Size: 1571 3159
XmnI GAAnn'nnTTC
Cuts at: 820 904 988 1156 1324 1492 1576 1660 820
Size: 84 84 168 168 168 84 84 3890
Fragments arranged by size:
3890 168 168 168 84 84 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmBI BssHII BstZ17I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI