(Circular) (Six-base) MAPSORT of: pF1KB8338.seq Check: 5423 from: 1 to: 3929 pF1KB8338 3929 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 967 967 Size: 3929 Acc65I G'GTAC_C Cuts at: 945 945 Size: 3929 AclI AA'CG_TT Cuts at: 3769 3769 Size: 3929 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 243 1754 2186 2933 3201 243 Size: 1511 432 747 268 971 Fragments arranged by size: 1511 971 747 432 268 AflIII A'CryG_T Cuts at: 791 1281 2385 3362 791 Size: 490 1104 977 1358 Fragments arranged by size: 1358 1104 977 490 AgeI A'CCGG_T Cuts at: 2317 2317 Size: 3929 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 445 445 Size: 3929 AlwNI CAG_nnn'CTG Cuts at: 762 1234 2801 762 Size: 472 1567 1890 Fragments arranged by size: 1890 1567 472 ApaI G_GGCC'C Cuts at: 126 126 Size: 3929 ApaLI G'TGCA_C Cuts at: 316 382 2699 316 Size: 66 2317 1546 Fragments arranged by size: 2317 1546 66 ApoI r'AATT_y Cuts at: 933 3282 3538 933 Size: 2349 256 1324 Fragments arranged by size: 2349 1324 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3929 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3929 AvaI C'yCGr_G Cuts at: 949 949 Size: 3929 BamHI G'GATC_C Cuts at: 954 954 Size: 3929 BanI G'GyrC_C Cuts at: 160 298 328 393 411 625 737 945 1359 Size: 138 30 65 18 214 112 208 414 Cuts at: 1359 1607 1642 160 Size: 248 35 2447 Fragments arranged by size: 2447 414 248 214 208 138 112 65 35 30 18 BanII G_rGCy'C Cuts at: 126 449 943 1973 126 Size: 323 494 1030 2082 Fragments arranged by size: 2082 1030 494 323 BbeI G_GCGC'C Cuts at: 164 302 332 397 415 629 1363 1611 164 Size: 138 30 65 18 214 734 248 2482 Fragments arranged by size: 2482 734 248 214 138 65 30 18 BciVI GTATCCnnnnn_n' Cuts at: 1827 2588 3929 1827 Size: 761 1341 1827 Fragments arranged by size: 1827 1341 761 BclI T'GATC_A Cuts at: 1449 1449 Size: 3929 BglI GCCn_nnn'nGGC Cuts at: 225 225 Size: 3929 BglII A'GATC_T Cuts at: 860 1444 3481 860 Size: 584 2037 1308 Fragments arranged by size: 2037 1308 584 BlpI GC'TnA_GC Cuts at: 181 1041 181 Size: 860 3069 Bme1580I G_kGCm'C Cuts at: 126 320 386 742 1554 1647 2703 126 Size: 194 66 356 812 93 1056 1352 Fragments arranged by size: 1352 1056 812 356 194 93 66 BmrI ACTGGGnnnn_n' Cuts at: 128 307 1141 1222 1557 3701 128 Size: 179 834 81 335 2144 356 Fragments arranged by size: 2144 834 356 335 179 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2296 2296 Size: 3929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 463 468 578 790 463 Size: 5 110 212 3602 Fragments arranged by size: 3602 212 110 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1105 1984 2476 2774 1105 Size: 879 492 298 2260 Fragments arranged by size: 2260 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3633 3633 Size: 3929 BsaAI yAC'GTr Cuts at: 141 381 1282 141 Size: 240 901 2788 Fragments arranged by size: 2788 901 240 BsaBI GATnn'nnATC Cuts at: 6 1443 3480 6 Size: 1437 2037 455 Fragments arranged by size: 2037 1437 455 BsaHI Gr'CG_yC Cuts at: 161 167 299 329 394 412 626 1360 1608 Size: 6 132 30 65 18 214 734 248 Cuts at: 1608 2310 3806 161 Size: 702 1496 284 Fragments arranged by size: 1496 734 702 284 248 214 132 65 30 18 6 BsaWI w'CCGG_w Cuts at: 1118 1639 2317 2591 2738 3149 1118 Size: 521 678 274 147 411 1898 Fragments arranged by size: 1898 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 418 448 763 793 418 Size: 30 315 30 3554 Fragments arranged by size: 3554 315 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 581 581 Size: 3929 BseYI C'CCAG_C Cuts at: 156 616 693 1354 2112 2689 3185 156 Size: 460 77 661 758 577 496 900 Fragments arranged by size: 900 758 661 577 496 460 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 288 288 Size: 3929 BsiEI CG_ry'CG Cuts at: 104 1471 1517 2725 104 Size: 1367 46 1208 1308 Fragments arranged by size: 1367 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 251 320 386 943 1721 1911 2703 251 Size: 69 66 557 778 190 792 1477 Fragments arranged by size: 1477 792 778 557 190 69 66 Bsp1286I G_dGCh'C Cuts at: 126 251 320 386 449 742 943 1554 1647 Size: 125 69 66 63 293 201 611 93 Cuts at: 1647 1721 1911 1973 2703 126 Size: 74 190 62 730 1352 Fragments arranged by size: 1352 730 611 293 201 190 125 93 74 69 66 63 62 BspEI T'CCGG_A Cuts at: 3149 3149 Size: 3929 BspHI T'CATG_A Cuts at: 3105 3105 Size: 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 413 803 843 979 1193 1495 1876 413 Size: 390 40 136 214 302 381 2466 Fragments arranged by size: 2466 390 381 302 214 136 40 BsrBI CCG'CTC Cuts at: 50 659 2221 2275 3759 3924 50 Size: 609 1562 54 1484 165 55 Fragments arranged by size: 1562 1484 609 165 55 54 BsrDI GCAATG_nn' Cuts at: 1841 1841 Size: 3929 BsrFI r'CCGG_y Cuts at: 488 1927 2108 2317 488 Size: 1439 181 209 2100 Fragments arranged by size: 2100 1439 209 181 BssHII G'CGCG_C Cuts at: 715 715 Size: 3929 BssSI C'ACGA_G Cuts at: 303 2200 2558 303 Size: 1897 358 1674 Fragments arranged by size: 1897 1674 358 BstAPI GCAn_nnn'nTGC Cuts at: 1306 1306 Size: 3929 BstBI TT'CG_AA Cuts at: 2290 2290 Size: 3929 BstYI r'GATC_y Cuts at: 1 860 954 1421 1444 1779 2025 3037 3123 Size: 859 94 467 23 335 246 1012 86 Cuts at: 3123 3135 3481 1 Size: 12 346 449 Fragments arranged by size: 1012 859 467 449 346 335 246 94 86 23 12 BtgI C'CryG_G Cuts at: 107 170 398 107 Size: 63 228 3638 Fragments arranged by size: 3638 228 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1879 2060 2223 1879 Size: 181 163 3585 Fragments arranged by size: 3585 181 163 BtsI GCAGTG_nn' Cuts at: 1172 1172 Size: 3929 ClaI AT'CG_AT Cuts at: 680 680 Size: 3929 DraI TTT'AAA Cuts at: 928 928 Size: 3929 DrdI GACnn_nn'nnGTC Cuts at: 1635 2493 1635 Size: 858 3071 EaeI y'GGCC_r Cuts at: 110 189 242 521 1514 1688 2079 2106 3451 Size: 79 53 279 993 174 391 27 1345 Cuts at: 3451 110 Size: 588 Fragments arranged by size: 1345 993 588 391 279 174 79 53 27 EagI C'GGCC_G Cuts at: 1514 1514 Size: 3929 EarI CTCTTCn'nnn_ Cuts at: 1952 2162 1952 Size: 210 3719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 677 2137 2447 2593 3739 677 Size: 1460 310 146 1146 867 Fragments arranged by size: 1460 1146 867 310 146 EcoICRI GAG'CTC Cuts at: 941 941 Size: 3929 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 243 463 468 578 790 1754 2186 2933 3201 Size: 220 5 110 212 964 432 747 268 Cuts at: 3201 243 Size: 971 Fragments arranged by size: 971 964 747 432 268 220 212 110 5 EcoO109I rG'GnC_Cy Cuts at: 122 123 206 1068 122 Size: 1 83 862 2983 Fragments arranged by size: 2983 862 83 1 EcoRI G'AATT_C Cuts at: 933 933 Size: 3929 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1948 1948 Size: 3929 FspI TGC'GCA Cuts at: 1710 1710 Size: 3929 HaeII r_GCGC'y Cuts at: 164 302 332 397 415 629 1363 1611 2633 Size: 138 30 65 18 214 734 248 1022 Cuts at: 2633 3612 164 Size: 979 481 Fragments arranged by size: 1022 979 734 481 248 214 138 65 30 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1771 1801 1803 1833 1771 Size: 30 2 30 3867 Fragments arranged by size: 3867 30 30 2 HincII GTy'rAC Cuts at: 968 968 Size: 3929 KasI G'GCGC_C Cuts at: 160 298 328 393 411 625 1359 1607 160 Size: 138 30 65 18 214 734 248 2482 Fragments arranged by size: 2482 734 248 214 138 65 30 18 KpnI G_GTAC'C Cuts at: 949 949 Size: 3929 MluI A'CGCG_T Cuts at: 3362 3362 Size: 3929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2600 2784 2600 Size: 184 3745 MscI TGG'CCA Cuts at: 112 1690 112 Size: 1578 2351 MslI CAynn'nnrTG Cuts at: 1918 2045 1918 Size: 127 3802 MspA1I CmG'CkG Cuts at: 172 346 516 1040 1354 1714 2727 2972 172 Size: 174 170 524 314 360 1013 245 1129 Fragments arranged by size: 1129 1013 524 360 314 245 174 170 NaeI GCC'GGC Cuts at: 490 2110 490 Size: 1620 2309 NarI GG'CG_CC Cuts at: 161 299 329 394 412 626 1360 1608 161 Size: 138 30 65 18 214 734 248 2482 Fragments arranged by size: 2482 734 248 214 138 65 30 18 NcoI C'CATG_G Cuts at: 107 107 Size: 3929 NgoMIV G'CCGG_C Cuts at: 488 2108 488 Size: 1620 2309 NspI r_CATG'y Cuts at: 432 795 982 1482 2389 432 Size: 363 187 500 907 1972 Fragments arranged by size: 1972 907 500 363 187 PasI CC'CwG_GG Cuts at: 126 210 126 Size: 84 3845 PciI A'CATG_T Cuts at: 791 2385 791 Size: 1594 2335 PflMI CCAn_nnn'nTGG Cuts at: 762 762 Size: 3929 PmeI GTTT'AAAC Cuts at: 928 928 Size: 3929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 769 801 3068 3100 769 Size: 32 2267 32 1598 Fragments arranged by size: 2267 1598 32 32 PpuMI rG'GwC_Cy Cuts at: 206 206 Size: 3929 PspOMI G'GGCC_C Cuts at: 122 122 Size: 3929 PstI C_TGCA'G Cuts at: 976 1661 976 Size: 685 3244 PvuI CG_AT'CG Cuts at: 104 104 Size: 3929 PvuII CAG'CTG Cuts at: 1354 1714 1354 Size: 360 3569 RsrII CG'GwC_CG Cuts at: 2124 2124 Size: 3929 SacI G_AGCT'C Cuts at: 943 943 Size: 3929 SacII CC_GC'GG Cuts at: 173 173 Size: 3929 SalI G'TCGA_C Cuts at: 966 966 Size: 3929 SanDI GG'GwC_CC Cuts at: 206 206 Size: 3929 SapI GCTCTTCn'nnn_ Cuts at: 1952 2162 1952 Size: 210 3719 SbfI CC_TGCA'GG Cuts at: 976 976 Size: 3929 ScaI AGT'ACT Cuts at: 3446 3446 Size: 3929 SfcI C'TryA_G Cuts at: 33 972 1657 2650 2841 33 Size: 939 685 993 191 1121 Fragments arranged by size: 1121 993 939 685 191 SfoI GGC'GCC Cuts at: 162 300 330 395 413 627 1361 1609 162 Size: 138 30 65 18 214 734 248 2482 Fragments arranged by size: 2482 734 248 214 138 65 30 18 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3929 SmaI CCC'GGG Cuts at: 951 951 Size: 3929 SmlI C'TyrA_G Cuts at: 1084 1999 2491 2753 1084 Size: 915 492 262 2260 Fragments arranged by size: 2260 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3929 SphI G_CATG'C Cuts at: 982 1482 982 Size: 500 3429 StyI C'CwwG_G Cuts at: 107 281 1063 1417 107 Size: 174 782 354 2619 Fragments arranged by size: 2619 782 354 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 923 1512 1645 2104 2313 923 Size: 589 133 459 209 2539 Fragments arranged by size: 2539 589 459 209 133 TatI w'GTAC_w Cuts at: 3444 3444 Size: 3929 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 547 579 3446 3478 547 Size: 32 2867 32 998 Fragments arranged by size: 2867 998 32 32 Tth111I GACn'n_nGTC Cuts at: 1726 1726 Size: 3929 XbaI T'CTAG_A Cuts at: 960 960 Size: 3929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 123 701 1424 123 Size: 578 723 2628 Fragments arranged by size: 2628 723 578 XmaI C'CCGG_G Cuts at: 949 949 Size: 3929 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI