Restriction map for
pF1KB8340
(Circular) (Six-base) MAPSORT of: pF1KB8340.seq Check: 7268 from: 1 to: 3332
pF1KB8340 3332 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 179 370 179
Size: 191 3141
Acc65I G'GTAC_C
Cuts at: 348 348
Size: 3332
AclI AA'CG_TT
Cuts at: 3172 3172
Size: 3332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1589 2336 2604 1157
Size: 432 747 268 1885
Fragments arranged by size:
1885 747 432 268
AflIII A'CryG_T
Cuts at: 684 1788 2765 684
Size: 1104 977 1251
Fragments arranged by size:
1251 1104 977
AgeI A'CCGG_T
Cuts at: 1720 1720
Size: 3332
AlwNI CAG_nnn'CTG
Cuts at: 270 637 2204 270
Size: 367 1567 1398
Fragments arranged by size:
1567 1398 367
ApaLI G'TGCA_C
Cuts at: 2102 2102
Size: 3332
ApoI r'AATT_y
Cuts at: 336 2685 2941 336
Size: 2349 256 727
Fragments arranged by size:
2349 727 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3332
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3332
AvaI C'yCGr_G
Cuts at: 352 352
Size: 3332
BamHI G'GATC_C
Cuts at: 357 357
Size: 3332
BanI G'GyrC_C
Cuts at: 348 762 1010 1045 348
Size: 414 248 35 2635
Fragments arranged by size:
2635 414 248 35
BanII G_rGCy'C
Cuts at: 346 1376 346
Size: 1030 2302
BbeI G_GCGC'C
Cuts at: 766 1014 766
Size: 248 3084
BciVI GTATCCnnnnn_n'
Cuts at: 1230 1991 3332 1230
Size: 761 1341 1230
Fragments arranged by size:
1341 1230 761
BclI T'GATC_A
Cuts at: 852 852
Size: 3332
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3332
BglII A'GATC_T
Cuts at: 847 2884 847
Size: 2037 1295
BlpI GC'TnA_GC
Cuts at: 444 444
Size: 3332
Bme1580I G_kGCm'C
Cuts at: 957 1050 2106 957
Size: 93 1056 2183
Fragments arranged by size:
2183 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 544 625 960 3104 544
Size: 81 335 2144 772
Fragments arranged by size:
2144 772 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 178
Size: 3332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1387 1879 2177 508
Size: 879 492 298 1663
Fragments arranged by size:
1663 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 112 238 3036 112
Size: 126 2798 408
Fragments arranged by size:
2798 408 126
BsaAI yAC'GTr
Cuts at: 685 685
Size: 3332
BsaBI GATnn'nnATC
Cuts at: 6 846 2883 6
Size: 840 2037 455
Fragments arranged by size:
2037 840 455
BsaHI Gr'CG_yC
Cuts at: 763 1011 1713 3209 763
Size: 248 702 1496 886
Fragments arranged by size:
1496 886 702 248
BsaWI w'CCGG_w
Cuts at: 521 1042 1720 1994 2141 2552 521
Size: 521 678 274 147 411 1301
Fragments arranged by size:
1301 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 256 253
Size: 3 3329
BseYI C'CCAG_C
Cuts at: 757 1515 2092 2588 757
Size: 758 577 496 1501
Fragments arranged by size:
1501 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 330
Size: 3332
BsiEI CG_ry'CG
Cuts at: 104 221 874 920 2128 104
Size: 117 653 46 1208 1308
Fragments arranged by size:
1308 1208 653 117 46
BsiHKAI G_wGCw'C
Cuts at: 346 1124 1314 2106 346
Size: 778 190 792 1572
Fragments arranged by size:
1572 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 346 957 1050 1124 1314 1376 2106 346
Size: 611 93 74 190 62 730 1572
Fragments arranged by size:
1572 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2552 2552
Size: 3332
BspHI T'CATG_A
Cuts at: 2508 2508
Size: 3332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 596 898 1279 382
Size: 214 302 381 2435
Fragments arranged by size:
2435 381 302 214
BsrBI CCG'CTC
Cuts at: 50 128 1624 1678 3162 3327 50
Size: 78 1496 54 1484 165 55
Fragments arranged by size:
1496 1484 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 1244 1244
Size: 3332
BsrFI r'CCGG_y
Cuts at: 1330 1511 1720 1330
Size: 181 209 2942
Fragments arranged by size:
2942 209 181
BssSI C'ACGA_G
Cuts at: 1603 1961 1603
Size: 358 2974
BstAPI GCAn_nnn'nTGC
Cuts at: 709 709
Size: 3332
BstBI TT'CG_AA
Cuts at: 1693 1693
Size: 3332
BstYI r'GATC_y
Cuts at: 1 313 357 824 847 1182 1428 2440 2526
Size: 312 44 467 23 335 246 1012 86
Cuts at: 2526 2538 2884 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 312 246 86
44 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 198 1282 1463 1626 198
Size: 1084 181 163 1904
Fragments arranged by size:
1904 1084 181 163
BtsI GCAGTG_nn'
Cuts at: 575 575
Size: 3332
DraI TTT'AAA
Cuts at: 331 331
Size: 3332
DraIII CAC_nnn'GTG
Cuts at: 206 206
Size: 3332
DrdI GACnn_nn'nnGTC
Cuts at: 1038 1896 1038
Size: 858 2474
EaeI y'GGCC_r
Cuts at: 218 917 1091 1482 1509 2854 218
Size: 699 174 391 27 1345 696
Fragments arranged by size:
1345 699 696 391 174 27
EagI C'GGCC_G
Cuts at: 218 917 218
Size: 699 2633
EarI CTCTTCn'nnn_
Cuts at: 1355 1565 1355
Size: 210 3122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 1540 1850 1996 3142 130
Size: 1410 310 146 1146 320
Fragments arranged by size:
1410 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 344 344
Size: 3332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 1157 1589 2336 2604 178
Size: 979 432 747 268 906
Fragments arranged by size:
979 906 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 235 235
Size: 3332
EcoO109I rG'GnC_Cy
Cuts at: 471 471
Size: 3332
EcoRI G'AATT_C
Cuts at: 336 336
Size: 3332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1351 1351
Size: 3332
FspI TGC'GCA
Cuts at: 1113 1113
Size: 3332
HaeII r_GCGC'y
Cuts at: 270 288 766 1014 2036 3015 270
Size: 18 478 248 1022 979 587
Fragments arranged by size:
1022 979 587 478 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1174 1204 1206 1236 298
Size: 32 844 30 2 30 2394
Fragments arranged by size:
2394 844 32 30 30 2
HincII GTy'rAC
Cuts at: 180 371 180
Size: 191 3141
KasI G'GCGC_C
Cuts at: 762 1010 762
Size: 248 3084
KpnI G_GTAC'C
Cuts at: 352 352
Size: 3332
MluI A'CGCG_T
Cuts at: 2765 2765
Size: 3332
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2187 2003
Size: 184 3148
MscI TGG'CCA
Cuts at: 1093 1093
Size: 3332
MslI CAynn'nnrTG
Cuts at: 1321 1448 1321
Size: 127 3205
MspA1I CmG'CkG
Cuts at: 443 757 1117 2130 2375 443
Size: 314 360 1013 245 1400
Fragments arranged by size:
1400 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1513 1513
Size: 3332
NarI GG'CG_CC
Cuts at: 763 1011 763
Size: 248 3084
NgoMIV G'CCGG_C
Cuts at: 1511 1511
Size: 3332
NspI r_CATG'y
Cuts at: 385 885 1792 385
Size: 500 907 1925
Fragments arranged by size:
1925 907 500
PciI A'CATG_T
Cuts at: 1788 1788
Size: 3332
PfoI T'CCnGG_A
Cuts at: 155 155
Size: 3332
PmeI GTTT'AAAC
Cuts at: 331 331
Size: 3332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2471 2503 2471
Size: 32 3300
PstI C_TGCA'G
Cuts at: 379 1064 379
Size: 685 2647
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3332
PvuII CAG'CTG
Cuts at: 757 1117 757
Size: 360 2972
RsrII CG'GwC_CG
Cuts at: 1527 1527
Size: 3332
SacI G_AGCT'C
Cuts at: 346 346
Size: 3332
SalI G'TCGA_C
Cuts at: 178 369 178
Size: 191 3141
SapI GCTCTTCn'nnn_
Cuts at: 1355 1565 1355
Size: 210 3122
SbfI CC_TGCA'GG
Cuts at: 379 379
Size: 3332
ScaI AGT'ACT
Cuts at: 2849 2849
Size: 3332
SexAI A'CCwGG_T
Cuts at: 143 143
Size: 3332
SfcI C'TryA_G
Cuts at: 33 375 1060 2053 2244 33
Size: 342 685 993 191 1121
Fragments arranged by size:
1121 993 685 342 191
SfoI GGC'GCC
Cuts at: 764 1012 764
Size: 248 3084
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3332
SmaI CCC'GGG
Cuts at: 354 354
Size: 3332
SmlI C'TyrA_G
Cuts at: 487 1402 1894 2156 487
Size: 915 492 262 1663
Fragments arranged by size:
1663 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3332
SphI G_CATG'C
Cuts at: 385 885 385
Size: 500 2832
StuI AGG'CCT
Cuts at: 230 296 230
Size: 66 3266
StyI C'CwwG_G
Cuts at: 466 820 466
Size: 354 2978
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 915 1048 1507 1716 915
Size: 133 459 209 2531
Fragments arranged by size:
2531 459 209 133
TatI w'GTAC_w
Cuts at: 161 2847 161
Size: 2686 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 2849 2881 178
Size: 32 2639 32 629
Fragments arranged by size:
2639 629 32 32
Tth111I GACn'n_nGTC
Cuts at: 1129 1129
Size: 3332
XbaI T'CTAG_A
Cuts at: 363 363
Size: 3332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 827 827
Size: 3332
XmaI C'CCGG_G
Cuts at: 352 352
Size: 3332
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI