Restriction map for
pF1KB8341
(Circular) (Six-base) MAPSORT of: pF1KB8341.seq Check: 4402 from: 1 to: 4940
pF1KB8341 4940 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1978 1978
Size: 4940
Acc65I G'GTAC_C
Cuts at: 1956 1956
Size: 4940
AclI AA'CG_TT
Cuts at: 4780 4780
Size: 4940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 2765 3197 3944 4212 629
Size: 2136 432 747 268 1357
Fragments arranged by size:
2136 1357 747 432 268
AflIII A'CryG_T
Cuts at: 428 1867 2292 3396 4373 428
Size: 1439 425 1104 977 995
Fragments arranged by size:
1439 1104 995 977 425
AgeI A'CCGG_T
Cuts at: 3328 3328
Size: 4940
AleI CACnn'nnGTG
Cuts at: 312 677 1682 312
Size: 365 1005 3570
Fragments arranged by size:
3570 1005 365
AlwNI CAG_nnn'CTG
Cuts at: 2245 3812 2245
Size: 1567 3373
ApaLI G'TGCA_C
Cuts at: 3710 3710
Size: 4940
ApoI r'AATT_y
Cuts at: 668 752 920 1004 1172 1340 1424 1508 1589
Size: 84 168 84 168 168 84 84 81
Cuts at: 1589 1673 1944 4293 4549 668
Size: 84 271 2349 256 1059
Fragments arranged by size:
2349 1059 271 256 168 168 168 84
84 84 84 84 81
AseI AT'TA_AT
Cuts at: 20 20
Size: 4940
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
AvaI C'yCGr_G
Cuts at: 1960 1960
Size: 4940
BamHI G'GATC_C
Cuts at: 1841 1965 1841
Size: 124 4816
BanI G'GyrC_C
Cuts at: 1956 2370 2618 2653 1956
Size: 414 248 35 4243
Fragments arranged by size:
4243 414 248 35
BanII G_rGCy'C
Cuts at: 1954 2984 1954
Size: 1030 3910
BbeI G_GCGC'C
Cuts at: 2374 2622 2374
Size: 248 4692
BbsI GAAGACnn'nnnn_
Cuts at: 1222 1555 1222
Size: 333 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4906
BciVI GTATCCnnnnn_n'
Cuts at: 2838 3599 4940 2838
Size: 761 1341 2838
Fragments arranged by size:
2838 1341 761
BclI T'GATC_A
Cuts at: 2460 2460
Size: 4940
BglII A'GATC_T
Cuts at: 2455 4492 2455
Size: 2037 2903
BlpI GC'TnA_GC
Cuts at: 2052 2052
Size: 4940
Bme1580I G_kGCm'C
Cuts at: 2565 2658 3714 2565
Size: 93 1056 3791
Fragments arranged by size:
3791 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2152 2233 2568 4712 2152
Size: 81 335 2144 2380
Fragments arranged by size:
2380 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 4940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 295 1787 263
Size: 32 1492 3416
Fragments arranged by size:
3416 1492 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 2995 3487 3785 2116
Size: 879 492 298 3271
Fragments arranged by size:
3271 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1266 4644 1266
Size: 3378 1562
BsaAI yAC'GTr
Cuts at: 1561 2293 1561
Size: 732 4208
BsaBI GATnn'nnATC
Cuts at: 6 2454 4491 6
Size: 2448 2037 455
Fragments arranged by size:
2448 2037 455
BsaHI Gr'CG_yC
Cuts at: 2371 2619 3321 4817 2371
Size: 248 702 1496 2494
Fragments arranged by size:
2494 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1348 2129 2650 3328 3602 3749 4160 1348
Size: 781 521 678 274 147 411 2128
Fragments arranged by size:
2128 781 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2365 3123 3700 4196 2365
Size: 758 577 496 3109
Fragments arranged by size:
3109 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2482 2528 3736 104
Size: 2378 46 1208 1308
Fragments arranged by size:
2378 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1954 2732 2922 3714 1954
Size: 778 190 792 3180
Fragments arranged by size:
3180 792 778 190
BsmI GAATG_Cn'
Cuts at: 175 541 1213 175
Size: 366 672 3902
Fragments arranged by size:
3902 672 366
Bsp1286I G_dGCh'C
Cuts at: 1954 2565 2658 2732 2922 2984 3714 1954
Size: 611 93 74 190 62 730 3180
Fragments arranged by size:
3180 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4160 4160
Size: 4940
BspHI T'CATG_A
Cuts at: 4116 4116
Size: 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1990 2204 2506 2887 1990
Size: 214 302 381 4043
Fragments arranged by size:
4043 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3232 3286 4770 4935 50
Size: 3182 54 1484 165 55
Fragments arranged by size:
3182 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2852 2852
Size: 4940
BsrFI r'CCGG_y
Cuts at: 2938 3119 3328 2938
Size: 181 209 4550
Fragments arranged by size:
4550 209 181
BsrGI T'GTAC_A
Cuts at: 197 521 197
Size: 324 4616
BssSI C'ACGA_G
Cuts at: 3211 3569 3211
Size: 358 4582
BstAPI GCAn_nnn'nTGC
Cuts at: 2317 2317
Size: 4940
BstBI TT'CG_AA
Cuts at: 3301 3301
Size: 4940
BstYI r'GATC_y
Cuts at: 1 1841 1965 2432 2455 2790 3036 4048 4134
Size: 1840 124 467 23 335 246 1012 86
Cuts at: 4134 4146 4492 1
Size: 12 346 449
Fragments arranged by size:
1840 1012 467 449 346 335 246 124
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2890 3071 3234 2890
Size: 181 163 4596
Fragments arranged by size:
4596 181 163
BtsI GCAGTG_nn'
Cuts at: 2183 2183
Size: 4940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1275 1310 1275
Size: 35 4905
DraI TTT'AAA
Cuts at: 1939 1939
Size: 4940
DrdI GACnn_nn'nnGTC
Cuts at: 2646 3504 2646
Size: 858 4082
EaeI y'GGCC_r
Cuts at: 140 2525 2699 3090 3117 4462 140
Size: 2385 174 391 27 1345 618
Fragments arranged by size:
2385 1345 618 391 174 27
EagI C'GGCC_G
Cuts at: 2525 2525
Size: 4940
EarI CTCTTCn'nnn_
Cuts at: 486 914 2963 3173 486
Size: 428 2049 210 2253
Fragments arranged by size:
2253 2049 428 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3148 3458 3604 4750 3148
Size: 310 146 1146 3338
Fragments arranged by size:
3338 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1952 1952
Size: 4940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 295 629 1787 2765 3197 3944 4212 263
Size: 32 334 1158 978 432 747 268 991
Fragments arranged by size:
1158 991 978 747 432 334 268 32
EcoO109I rG'GnC_Cy
Cuts at: 1802 2079 1802
Size: 277 4663
EcoRI G'AATT_C
Cuts at: 668 920 1004 1172 1340 1424 1589 1673 1944
Size: 252 84 168 168 84 165 84 271
Cuts at: 1944 668
Size: 3664
Fragments arranged by size:
3664 271 252 168 168 165 84 84
84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2959 2959
Size: 4940
FspI TGC'GCA
Cuts at: 2721 2721
Size: 4940
HaeII r_GCGC'y
Cuts at: 2374 2622 3644 4623 2374
Size: 248 1022 979 2691
Fragments arranged by size:
2691 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 235 267 2782 2812 2814 2844 235
Size: 32 2515 30 2 30 2331
Fragments arranged by size:
2515 2331 32 30 30 2
HincII GTy'rAC
Cuts at: 1979 1979
Size: 4940
HindIII A'AGCT_T
Cuts at: 797 797
Size: 4940
KasI G'GCGC_C
Cuts at: 2370 2618 2370
Size: 248 4692
KpnI G_GTAC'C
Cuts at: 1960 1960
Size: 4940
MluI A'CGCG_T
Cuts at: 4373 4373
Size: 4940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3611 3795 194
Size: 3417 184 1339
Fragments arranged by size:
3417 1339 184
MscI TGG'CCA
Cuts at: 142 2701 142
Size: 2559 2381
MslI CAynn'nnrTG
Cuts at: 312 677 761 861 929 1013 1097 1265 1433
Size: 365 84 100 68 84 84 168 168
Cuts at: 1433 1598 1682 1850 2929 3056 312
Size: 165 84 168 1079 127 2196
Fragments arranged by size:
2196 1079 365 168 168 168 165 127
100 84 84 84 84 68
MspA1I CmG'CkG
Cuts at: 2051 2365 2725 3738 3983 2051
Size: 314 360 1013 245 3008
Fragments arranged by size:
3008 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3121 3121
Size: 4940
NarI GG'CG_CC
Cuts at: 2371 2619 2371
Size: 248 4692
NdeI CA'TA_TG
Cuts at: 858 1114 858
Size: 256 4684
NgoMIV G'CCGG_C
Cuts at: 3119 3119
Size: 4940
NspI r_CATG'y
Cuts at: 432 791 1871 1993 2493 3400 432
Size: 359 1080 122 500 907 1972
Fragments arranged by size:
1972 1080 907 500 359 122
PciI A'CATG_T
Cuts at: 428 1867 3396 428
Size: 1439 1529 1972
Fragments arranged by size:
1972 1529 1439
PflMI CCAn_nnn'nTGG
Cuts at: 120 120
Size: 4940
PmeI GTTT'AAAC
Cuts at: 1939 1939
Size: 4940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 4079
Size: 32 4908
PsiI TTA'TAA
Cuts at: 1280 1280
Size: 4940
PstI C_TGCA'G
Cuts at: 1987 2672 1987
Size: 685 4255
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4940
PvuII CAG'CTG
Cuts at: 2365 2725 2365
Size: 360 4580
RsrII CG'GwC_CG
Cuts at: 3135 3135
Size: 4940
SacI G_AGCT'C
Cuts at: 1954 1954
Size: 4940
SalI G'TCGA_C
Cuts at: 1977 1977
Size: 4940
SapI GCTCTTCn'nnn_
Cuts at: 2963 3173 2963
Size: 210 4730
SbfI CC_TGCA'GG
Cuts at: 1987 1987
Size: 4940
ScaI AGT'ACT
Cuts at: 4457 4457
Size: 4940
SfcI C'TryA_G
Cuts at: 33 222 1983 2668 3661 3852 33
Size: 189 1761 685 993 191 1121
Fragments arranged by size:
1761 1121 993 685 191 189
SfoI GGC'GCC
Cuts at: 2372 2620 2372
Size: 248 4692
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
SmaI CCC'GGG
Cuts at: 1962 1962
Size: 4940
SmlI C'TyrA_G
Cuts at: 2095 3010 3502 3764 2095
Size: 915 492 262 3271
Fragments arranged by size:
3271 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4940
SphI G_CATG'C
Cuts at: 1993 2493 1993
Size: 500 4440
StuI AGG'CCT
Cuts at: 715 967 1051 1387 1471 1636 715
Size: 252 84 336 84 165 4019
Fragments arranged by size:
4019 336 252 165 84 84
StyI C'CwwG_G
Cuts at: 509 2074 2428 509
Size: 1565 354 3021
Fragments arranged by size:
3021 1565 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2523 2656 3115 3324 2523
Size: 133 459 209 4139
Fragments arranged by size:
4139 459 209 133
TatI w'GTAC_w
Cuts at: 197 521 4455 197
Size: 324 3934 682
Fragments arranged by size:
3934 682 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4457 4489 4457
Size: 32 4908
Tth111I GACn'n_nGTC
Cuts at: 2737 2737
Size: 4940
XbaI T'CTAG_A
Cuts at: 1971 1971
Size: 4940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2435 2435
Size: 4940
XmaI C'CCGG_G
Cuts at: 1960 1960
Size: 4940
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI