(Circular) (Six-base) MAPSORT of: pF1KB8342.seq Check: 8678 from: 1 to: 4157 pF1KB8342 4157 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 1195 1195 Size: 4157 Acc65I G'GTAC_C Cuts at: 1173 1173 Size: 4157 AclI AA'CG_TT Cuts at: 3997 3997 Size: 4157 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1982 2414 3161 3429 1982 Size: 432 747 268 2710 Fragments arranged by size: 2710 747 432 268 AflIII A'CryG_T Cuts at: 236 725 1509 2613 3590 236 Size: 489 784 1104 977 803 Fragments arranged by size: 1104 977 803 784 489 AgeI A'CCGG_T Cuts at: 2545 2545 Size: 4157 AlwNI CAG_nnn'CTG Cuts at: 1462 3029 1462 Size: 1567 2590 ApaI G_GGCC'C Cuts at: 428 428 Size: 4157 ApaLI G'TGCA_C Cuts at: 2927 2927 Size: 4157 ApoI r'AATT_y Cuts at: 1161 3510 3766 1161 Size: 2349 256 1552 Fragments arranged by size: 2349 1552 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4157 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4157 AvaI C'yCGr_G Cuts at: 646 1177 646 Size: 531 3626 AvrII C'CTAG_G Cuts at: 369 369 Size: 4157 BamHI G'GATC_C Cuts at: 1182 1182 Size: 4157 BanI G'GyrC_C Cuts at: 903 1173 1587 1835 1870 903 Size: 270 414 248 35 3190 Fragments arranged by size: 3190 414 270 248 35 BanII G_rGCy'C Cuts at: 428 949 1171 2201 428 Size: 521 222 1030 2384 Fragments arranged by size: 2384 1030 521 222 BbeI G_GCGC'C Cuts at: 1591 1839 1591 Size: 248 3909 BbsI GAAGACnn'nnnn_ Cuts at: 411 780 411 Size: 369 3788 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 159 193 159 Size: 34 4123 BciVI GTATCCnnnnn_n' Cuts at: 403 2055 2816 4157 403 Size: 1652 761 1341 403 Fragments arranged by size: 1652 1341 761 403 BclI T'GATC_A Cuts at: 1677 1677 Size: 4157 BglI GCCn_nnn'nGGC Cuts at: 919 919 Size: 4157 BglII A'GATC_T Cuts at: 1672 3709 1672 Size: 2037 2120 BlpI GC'TnA_GC Cuts at: 203 1269 203 Size: 1066 3091 Bme1580I G_kGCm'C Cuts at: 143 428 908 1782 1875 2931 143 Size: 285 480 874 93 1056 1369 Fragments arranged by size: 1369 1056 874 480 285 93 BmrI ACTGGGnnnn_n' Cuts at: 1099 1369 1450 1785 3929 1099 Size: 270 81 335 2144 1327 Fragments arranged by size: 2144 1327 335 270 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2524 2524 Size: 4157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 673 1333 2212 2704 3002 673 Size: 660 879 492 298 1828 Fragments arranged by size: 1828 879 660 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3861 3861 Size: 4157 BsaAI yAC'GTr Cuts at: 138 516 1510 138 Size: 378 994 2785 Fragments arranged by size: 2785 994 378 BsaBI GATnn'nnATC Cuts at: 6 760 1671 3708 6 Size: 754 911 2037 455 Fragments arranged by size: 2037 911 754 455 BsaHI Gr'CG_yC Cuts at: 1588 1836 2538 4034 1588 Size: 248 702 1496 1711 Fragments arranged by size: 1711 1496 702 248 BsaWI w'CCGG_w Cuts at: 1046 1346 1867 2545 2819 2966 3377 1046 Size: 300 521 678 274 147 411 1826 Fragments arranged by size: 1826 678 521 411 300 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 931 961 931 Size: 30 4127 BseYI C'CCAG_C Cuts at: 255 812 899 978 1582 2340 2917 3413 255 Size: 557 87 79 604 758 577 496 999 Fragments arranged by size: 999 758 604 577 557 496 87 79 BsiEI CG_ry'CG Cuts at: 104 1151 1699 1745 2953 104 Size: 1047 548 46 1208 1308 Fragments arranged by size: 1308 1208 1047 548 46 BsiHKAI G_wGCw'C Cuts at: 664 1171 1949 2139 2931 664 Size: 507 778 190 792 1890 Fragments arranged by size: 1890 792 778 507 190 BsmI GAATG_Cn' Cuts at: 922 922 Size: 4157 BsmBI CGTCTCn'nnnn_ Cuts at: 1123 1123 Size: 4157 Bsp1286I G_dGCh'C Cuts at: 143 428 664 908 949 1171 1782 1875 1949 Size: 285 236 244 41 222 611 93 74 Cuts at: 1949 2139 2201 2931 143 Size: 190 62 730 1369 Fragments arranged by size: 1369 730 611 285 244 236 222 190 93 74 62 41 BspEI T'CCGG_A Cuts at: 1046 3377 1046 Size: 2331 1826 BspHI T'CATG_A Cuts at: 968 1028 3333 968 Size: 60 2305 1792 Fragments arranged by size: 2305 1792 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1207 1421 1723 2104 1207 Size: 214 302 381 3260 Fragments arranged by size: 3260 381 302 214 BsrBI CCG'CTC Cuts at: 50 510 2449 2503 3987 4152 50 Size: 460 1939 54 1484 165 55 Fragments arranged by size: 1939 1484 460 165 55 54 BsrDI GCAATG_nn' Cuts at: 2069 2069 Size: 4157 BsrFI r'CCGG_y Cuts at: 625 2155 2336 2545 625 Size: 1530 181 209 2237 Fragments arranged by size: 2237 1530 209 181 BssSI C'ACGA_G Cuts at: 663 2428 2786 663 Size: 1765 358 2034 Fragments arranged by size: 2034 1765 358 BstAPI GCAn_nnn'nTGC Cuts at: 1534 1534 Size: 4157 BstBI TT'CG_AA Cuts at: 2518 2518 Size: 4157 BstEII G'GTnAC_C Cuts at: 855 855 Size: 4157 BstYI r'GATC_y Cuts at: 1 1182 1649 1672 2007 2253 3265 3351 3363 Size: 1181 467 23 335 246 1012 86 12 Cuts at: 3363 3709 1 Size: 346 449 Fragments arranged by size: 1181 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 208 347 208 Size: 139 4018 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 867 2107 2288 2451 867 Size: 1240 181 163 2573 Fragments arranged by size: 2573 1240 181 163 BtsI GCAGTG_nn' Cuts at: 664 1400 664 Size: 736 3421 DraI TTT'AAA Cuts at: 1156 1156 Size: 4157 DraIII CAC_nnn'GTG Cuts at: 1093 1093 Size: 4157 DrdI GACnn_nn'nnGTC Cuts at: 1863 2721 1863 Size: 858 3299 EaeI y'GGCC_r Cuts at: 227 500 1148 1742 1916 2307 2334 3679 227 Size: 273 648 594 174 391 27 1345 705 Fragments arranged by size: 1345 705 648 594 391 273 174 27 EagI C'GGCC_G Cuts at: 1148 1742 1148 Size: 594 3563 EarI CTCTTCn'nnn_ Cuts at: 364 2180 2390 364 Size: 1816 210 2131 Fragments arranged by size: 2131 1816 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2365 2675 2821 3967 2365 Size: 310 146 1146 2555 Fragments arranged by size: 2555 1146 310 146 EcoICRI GAG'CTC Cuts at: 1169 1169 Size: 4157 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1982 2414 3161 3429 1982 Size: 432 747 268 2710 Fragments arranged by size: 2710 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 124 651 1296 124 Size: 527 645 2985 Fragments arranged by size: 2985 645 527 EcoRI G'AATT_C Cuts at: 1161 1161 Size: 4157 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 4157 FspI TGC'GCA Cuts at: 313 1938 313 Size: 1625 2532 HaeII r_GCGC'y Cuts at: 601 1001 1591 1839 2861 3840 601 Size: 400 590 248 1022 979 918 Fragments arranged by size: 1022 979 918 590 400 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 401 433 1090 1122 1999 2029 2031 2061 401 Size: 32 657 32 877 30 2 30 2497 Fragments arranged by size: 2497 877 657 32 32 30 30 2 HincII GTy'rAC Cuts at: 702 1196 702 Size: 494 3663 KasI G'GCGC_C Cuts at: 1587 1835 1587 Size: 248 3909 KpnI G_GTAC'C Cuts at: 1177 1177 Size: 4157 MluI A'CGCG_T Cuts at: 3590 3590 Size: 4157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 974 2828 3012 974 Size: 1854 184 2119 Fragments arranged by size: 2119 1854 184 MscI TGG'CCA Cuts at: 502 1918 502 Size: 1416 2741 MslI CAynn'nnrTG Cuts at: 724 730 751 862 874 967 2146 2273 724 Size: 6 21 111 12 93 1179 127 2608 Fragments arranged by size: 2608 1179 127 111 93 21 12 6 MspA1I CmG'CkG Cuts at: 808 1268 1582 1942 2955 3200 808 Size: 460 314 360 1013 245 1765 Fragments arranged by size: 1765 1013 460 360 314 245 NaeI GCC'GGC Cuts at: 627 2338 627 Size: 1711 2446 NarI GG'CG_CC Cuts at: 1588 1836 1588 Size: 248 3909 NcoI C'CATG_G Cuts at: 208 208 Size: 4157 NgoMIV G'CCGG_C Cuts at: 625 2336 625 Size: 1711 2446 NspI r_CATG'y Cuts at: 240 729 1059 1210 1710 2617 240 Size: 489 330 151 500 907 1780 Fragments arranged by size: 1780 907 500 489 330 151 PasI CC'CwG_GG Cuts at: 462 462 Size: 4157 PciI A'CATG_T Cuts at: 236 725 2613 236 Size: 489 1888 1780 Fragments arranged by size: 1888 1780 489 PmeI GTTT'AAAC Cuts at: 1156 1156 Size: 4157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3296 3328 3296 Size: 32 4125 PspOMI G'GGCC_C Cuts at: 424 424 Size: 4157 PstI C_TGCA'G Cuts at: 659 1204 1889 659 Size: 545 685 2927 Fragments arranged by size: 2927 685 545 PvuI CG_AT'CG Cuts at: 104 104 Size: 4157 PvuII CAG'CTG Cuts at: 1582 1942 1582 Size: 360 3797 RsrII CG'GwC_CG Cuts at: 2352 2352 Size: 4157 SacI G_AGCT'C Cuts at: 1171 1171 Size: 4157 SalI G'TCGA_C Cuts at: 1194 1194 Size: 4157 SapI GCTCTTCn'nnn_ Cuts at: 2180 2390 2180 Size: 210 3947 SbfI CC_TGCA'GG Cuts at: 1204 1204 Size: 4157 ScaI AGT'ACT Cuts at: 193 3674 193 Size: 3481 676 SfcI C'TryA_G Cuts at: 33 655 1200 1885 2878 3069 33 Size: 622 545 685 993 191 1121 Fragments arranged by size: 1121 993 685 622 545 191 SfoI GGC'GCC Cuts at: 1589 1837 1589 Size: 248 3909 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4157 SmaI CCC'GGG Cuts at: 1179 1179 Size: 4157 SmlI C'TyrA_G Cuts at: 688 1312 2227 2719 2981 688 Size: 624 915 492 262 1864 Fragments arranged by size: 1864 915 624 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4157 SphI G_CATG'C Cuts at: 1059 1210 1710 1059 Size: 151 500 3506 Fragments arranged by size: 3506 500 151 StuI AGG'CCT Cuts at: 1105 1105 Size: 4157 StyI C'CwwG_G Cuts at: 208 369 488 1291 1645 208 Size: 161 119 803 354 2720 Fragments arranged by size: 2720 803 354 161 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 891 946 952 1081 1740 1873 2332 2541 891 Size: 55 6 129 659 133 459 209 2507 Fragments arranged by size: 2507 659 459 209 133 129 55 6 TatI w'GTAC_w Cuts at: 191 3672 191 Size: 3481 676 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 931 963 3674 3706 931 Size: 32 2711 32 1382 Fragments arranged by size: 2711 1382 32 32 Tth111I GACn'n_nGTC Cuts at: 1954 1954 Size: 4157 XbaI T'CTAG_A Cuts at: 1188 1188 Size: 4157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 626 1652 626 Size: 1026 3131 XmaI C'CCGG_G Cuts at: 1177 1177 Size: 4157 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI