(Circular) (Six-base) MAPSORT of: pF1KB8345.seq Check: 9145 from: 1 to: 4997 pF1KB8345 4997 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 2035 2035 Size: 4997 Acc65I G'GTAC_C Cuts at: 2013 2013 Size: 4997 AclI AA'CG_TT Cuts at: 916 4837 916 Size: 3921 1076 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1053 1473 1553 1916 2822 3254 4001 4269 1053 Size: 420 80 363 906 432 747 268 1781 Fragments arranged by size: 1781 906 747 432 420 363 268 80 AflII C'TTAA_G Cuts at: 1842 1842 Size: 4997 AflIII A'CryG_T Cuts at: 545 1515 1683 1851 2349 3453 4430 545 Size: 970 168 168 498 1104 977 1112 Fragments arranged by size: 1112 1104 977 970 498 168 168 AgeI A'CCGG_T Cuts at: 3385 3385 Size: 4997 AlwNI CAG_nnn'CTG Cuts at: 225 2302 3869 225 Size: 2077 1567 1353 Fragments arranged by size: 2077 1567 1353 ApaLI G'TGCA_C Cuts at: 1357 3767 1357 Size: 2410 2587 ApoI r'AATT_y Cuts at: 365 512 1915 2001 4350 4606 365 Size: 147 1403 86 2349 256 756 Fragments arranged by size: 2349 1403 756 256 147 86 AseI AT'TA_AT Cuts at: 20 20 Size: 4997 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4997 AvaI C'yCGr_G Cuts at: 2017 2017 Size: 4997 BamHI G'GATC_C Cuts at: 2022 2022 Size: 4997 BanI G'GyrC_C Cuts at: 2013 2427 2675 2710 2013 Size: 414 248 35 4300 Fragments arranged by size: 4300 414 248 35 BanII G_rGCy'C Cuts at: 2011 3041 2011 Size: 1030 3967 BbeI G_GCGC'C Cuts at: 2431 2679 2431 Size: 248 4749 BbsI GAAGACnn'nnnn_ Cuts at: 552 930 1014 1098 1182 1237 1266 1541 1731 Size: 378 84 84 84 55 29 275 190 Cuts at: 1731 552 Size: 3818 Fragments arranged by size: 3818 378 275 190 84 84 84 55 29 BbvCI CC'TCA_GC Cuts at: 478 478 Size: 4997 BciVI GTATCCnnnnn_n' Cuts at: 2895 3656 4997 2895 Size: 761 1341 2895 Fragments arranged by size: 2895 1341 761 BclI T'GATC_A Cuts at: 459 1961 2517 459 Size: 1502 556 2939 Fragments arranged by size: 2939 1502 556 BfrBI ATG'CAT Cuts at: 567 675 567 Size: 108 4889 BglII A'GATC_T Cuts at: 1956 2512 4549 1956 Size: 556 2037 2404 Fragments arranged by size: 2404 2037 556 BlpI GC'TnA_GC Cuts at: 2109 2109 Size: 4997 Bme1580I G_kGCm'C Cuts at: 1361 2622 2715 3771 1361 Size: 1261 93 1056 2587 Fragments arranged by size: 2587 1261 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1242 2209 2290 2625 4769 1242 Size: 967 81 335 2144 1470 Fragments arranged by size: 2144 1470 967 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 493 3364 493 Size: 2871 2126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 172 229 542 1049 1133 1147 1217 1385 1805 Size: 57 313 507 84 14 70 168 420 Cuts at: 1805 172 Size: 3364 Fragments arranged by size: 3364 507 420 313 168 84 70 57 14 Bpu10I CC'TnA_GC Cuts at: 478 478 Size: 4997 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 568 813 2173 3052 3544 3842 568 Size: 245 1360 879 492 298 1723 Fragments arranged by size: 1723 1360 879 492 298 245 BsaI GGTCTCn'nnnn_ Cuts at: 205 693 4701 205 Size: 488 4008 501 Fragments arranged by size: 4008 501 488 BsaAI yAC'GTr Cuts at: 548 2350 548 Size: 1802 3195 BsaBI GATnn'nnATC Cuts at: 6 2511 4548 6 Size: 2505 2037 455 Fragments arranged by size: 2505 2037 455 BsaHI Gr'CG_yC Cuts at: 2428 2676 3378 4874 2428 Size: 248 702 1496 2551 Fragments arranged by size: 2551 1496 702 248 BsaWI w'CCGG_w Cuts at: 859 2186 2707 3385 3659 3806 4217 859 Size: 1327 521 678 274 147 411 1639 Fragments arranged by size: 1639 1327 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1318 1348 1318 Size: 30 4967 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 169 1323 169 Size: 1154 3843 BseYI C'CCAG_C Cuts at: 2422 3180 3757 4253 2422 Size: 758 577 496 3166 Fragments arranged by size: 3166 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1762 1944 1762 Size: 182 4815 BsiEI CG_ry'CG Cuts at: 104 2539 2585 3793 104 Size: 2435 46 1208 1308 Fragments arranged by size: 2435 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1361 2011 2789 2979 3771 1361 Size: 650 778 190 792 2587 Fragments arranged by size: 2587 792 778 650 190 BsiWI C'GTAC_G Cuts at: 548 548 Size: 4997 BsmI GAATG_Cn' Cuts at: 677 1135 1219 1303 1318 1529 1555 1822 677 Size: 458 84 84 15 211 26 267 3852 Fragments arranged by size: 3852 458 267 211 84 84 26 15 Bsp1286I G_dGCh'C Cuts at: 1361 2011 2622 2715 2789 2979 3041 3771 1361 Size: 650 611 93 74 190 62 730 2587 Fragments arranged by size: 2587 730 650 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 4217 4217 Size: 4997 BspHI T'CATG_A Cuts at: 4173 4173 Size: 4997 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2047 2261 2563 2944 2047 Size: 214 302 381 4100 Fragments arranged by size: 4100 381 302 214 BsrBI CCG'CTC Cuts at: 50 3289 3343 4827 4992 50 Size: 3239 54 1484 165 55 Fragments arranged by size: 3239 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 808 1396 2909 808 Size: 588 1513 2896 Fragments arranged by size: 2896 1513 588 BsrFI r'CCGG_y Cuts at: 2995 3176 3385 2995 Size: 181 209 4607 Fragments arranged by size: 4607 209 181 BsrGI T'GTAC_A Cuts at: 503 503 Size: 4997 BssSI C'ACGA_G Cuts at: 3268 3626 3268 Size: 358 4639 BstAPI GCAn_nnn'nTGC Cuts at: 1307 2374 1307 Size: 1067 3930 BstBI TT'CG_AA Cuts at: 921 1005 1089 1173 1257 3358 921 Size: 84 84 84 84 2101 2560 Fragments arranged by size: 2560 2101 84 84 84 84 BstXI CCAn_nnnn'nTGG Cuts at: 637 637 Size: 4997 BstYI r'GATC_y Cuts at: 1 1956 2022 2489 2512 2847 3093 4105 4191 Size: 1955 66 467 23 335 246 1012 86 Cuts at: 4191 4203 4549 1 Size: 12 346 449 Fragments arranged by size: 1955 1012 467 449 346 335 246 86 66 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4997 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 553 2947 3128 3291 553 Size: 2394 181 163 2259 Fragments arranged by size: 2394 2259 181 163 BtsI GCAGTG_nn' Cuts at: 773 1480 1648 1932 2240 773 Size: 707 168 284 308 3530 Fragments arranged by size: 3530 707 308 284 168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1122 1157 1206 1241 1374 1409 1542 1577 1626 Size: 35 49 35 133 35 133 35 49 Cuts at: 1626 1661 1122 Size: 35 4458 Fragments arranged by size: 4458 133 133 49 49 35 35 35 35 35 DraI TTT'AAA Cuts at: 1996 1996 Size: 4997 DrdI GACnn_nn'nnGTC Cuts at: 118 2703 3561 118 Size: 2585 858 1554 Fragments arranged by size: 2585 1554 858 EaeI y'GGCC_r Cuts at: 1747 2582 2756 3147 3174 4519 1747 Size: 835 174 391 27 1345 2225 Fragments arranged by size: 2225 1345 835 391 174 27 EagI C'GGCC_G Cuts at: 2582 2582 Size: 4997 EarI CTCTTCn'nnn_ Cuts at: 3020 3230 3020 Size: 210 4787 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3205 3515 3661 4807 3205 Size: 310 146 1146 3395 Fragments arranged by size: 3395 1146 310 146 EcoICRI GAG'CTC Cuts at: 2009 2009 Size: 4997 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 172 229 542 1049 1053 1133 1147 1217 1385 Size: 57 313 507 4 80 14 70 168 Cuts at: 1385 1473 1553 1805 1916 2822 3254 4001 4269 Size: 88 80 252 111 906 432 747 268 Cuts at: 4269 172 Size: 900 Fragments arranged by size: 906 900 747 507 432 313 268 252 168 111 88 80 80 70 57 14 4 EcoO109I rG'GnC_Cy Cuts at: 186 2136 186 Size: 1950 3047 EcoRI G'AATT_C Cuts at: 1915 2001 1915 Size: 86 4911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3016 3016 Size: 4997 FspI TGC'GCA Cuts at: 2778 2778 Size: 4997 HaeII r_GCGC'y Cuts at: 2431 2679 3701 4680 2431 Size: 248 1022 979 2748 Fragments arranged by size: 2748 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 477 509 1946 1978 2839 2869 2871 2901 477 Size: 32 1437 32 861 30 2 30 2573 Fragments arranged by size: 2573 1437 861 32 32 30 30 2 HincII GTy'rAC Cuts at: 2036 2036 Size: 4997 HindIII A'AGCT_T Cuts at: 896 1568 1652 896 Size: 672 84 4241 Fragments arranged by size: 4241 672 84 KasI G'GCGC_C Cuts at: 2427 2675 2427 Size: 248 4749 KpnI G_GTAC'C Cuts at: 2017 2017 Size: 4997 MfeI C'AATT_G Cuts at: 1407 1827 1407 Size: 420 4577 MluI A'CGCG_T Cuts at: 4430 4430 Size: 4997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 416 3668 3852 416 Size: 3252 184 1561 Fragments arranged by size: 3252 1561 184 MscI TGG'CCA Cuts at: 1749 2758 1749 Size: 1009 3988 MslI CAynn'nnrTG Cuts at: 624 876 960 1044 1354 2986 3113 624 Size: 252 84 84 310 1632 127 2508 Fragments arranged by size: 2508 1632 310 252 127 84 84 MspA1I CmG'CkG Cuts at: 2108 2422 2782 3795 4040 2108 Size: 314 360 1013 245 3065 Fragments arranged by size: 3065 1013 360 314 245 NaeI GCC'GGC Cuts at: 3178 3178 Size: 4997 NarI GG'CG_CC Cuts at: 2428 2676 2428 Size: 248 4749 NcoI C'CATG_G Cuts at: 107 107 Size: 4997 NdeI CA'TA_TG Cuts at: 1013 1433 1601 1769 1013 Size: 420 168 168 4241 Fragments arranged by size: 4241 420 168 168 NgoMIV G'CCGG_C Cuts at: 3176 3176 Size: 4997 NsiI A_TGCA'T Cuts at: 569 677 569 Size: 108 4889 NspI r_CATG'y Cuts at: 679 763 1519 1687 1855 2050 2550 3457 679 Size: 84 756 168 168 195 500 907 2219 Fragments arranged by size: 2219 907 756 500 195 168 168 84 PciI A'CATG_T Cuts at: 1515 1683 1851 3453 1515 Size: 168 168 1602 3059 Fragments arranged by size: 3059 1602 168 168 PmeI GTTT'AAAC Cuts at: 1996 1996 Size: 4997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 214 246 4136 4168 214 Size: 32 3890 32 1043 Fragments arranged by size: 3890 1043 32 32 PpuMI rG'GwC_Cy Cuts at: 186 186 Size: 4997 PstI C_TGCA'G Cuts at: 2044 2729 2044 Size: 685 4312 PvuI CG_AT'CG Cuts at: 104 104 Size: 4997 PvuII CAG'CTG Cuts at: 2422 2782 2422 Size: 360 4637 RsrII CG'GwC_CG Cuts at: 3192 3192 Size: 4997 SacI G_AGCT'C Cuts at: 2011 2011 Size: 4997 SalI G'TCGA_C Cuts at: 2034 2034 Size: 4997 SapI GCTCTTCn'nnn_ Cuts at: 3020 3230 3020 Size: 210 4787 SbfI CC_TGCA'GG Cuts at: 2044 2044 Size: 4997 ScaI AGT'ACT Cuts at: 4514 4514 Size: 4997 SfcI C'TryA_G Cuts at: 33 192 648 1700 1868 2040 2725 3718 3909 Size: 159 456 1052 168 172 685 993 191 Cuts at: 3909 33 Size: 1121 Fragments arranged by size: 1121 1052 993 685 456 191 172 168 159 SfoI GGC'GCC Cuts at: 2429 2677 2429 Size: 248 4749 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4997 SmaI CCC'GGG Cuts at: 2019 2019 Size: 4997 SmlI C'TyrA_G Cuts at: 583 828 1842 2152 3067 3559 3821 583 Size: 245 1014 310 915 492 262 1759 Fragments arranged by size: 1759 1014 915 492 310 262 245 SpeI A'CTAG_T Cuts at: 67 838 67 Size: 771 4226 SphI G_CATG'C Cuts at: 2050 2550 2050 Size: 500 4497 StuI AGG'CCT Cuts at: 646 1066 646 Size: 420 4577 StyI C'CwwG_G Cuts at: 107 305 2131 2485 107 Size: 198 1826 354 2619 Fragments arranged by size: 2619 1826 354 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2580 2713 3172 3381 2580 Size: 133 459 209 4196 Fragments arranged by size: 4196 459 209 133 TatI w'GTAC_w Cuts at: 503 4512 503 Size: 4009 988 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1263 1295 1318 1350 4514 4546 1263 Size: 32 23 32 3164 32 1714 Fragments arranged by size: 3164 1714 32 32 32 23 Tth111I GACn'n_nGTC Cuts at: 2794 2794 Size: 4997 XbaI T'CTAG_A Cuts at: 2028 2028 Size: 4997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 2492 114 Size: 2378 2619 XmaI C'CCGG_G Cuts at: 2017 2017 Size: 4997 XmnI GAAnn'nnTTC Cuts at: 339 898 1150 1187 1234 1318 1402 1486 1570 Size: 559 252 37 47 84 84 84 84 Cuts at: 1570 1654 1738 1822 339 Size: 84 84 84 3514 Fragments arranged by size: 3514 559 252 84 84 84 84 84 84 84 47 37 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI NheI NotI NruI PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI