Restriction map for
pF1KB8346
(Circular) (Six-base) MAPSORT of: pF1KB8346.seq Check: 4648 from: 1 to: 3494
pF1KB8346 3494 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2498 2766 1319
Size: 432 747 268 2047
Fragments arranged by size:
2047 747 432 268
AflIII A'CryG_T
Cuts at: 209 846 1950 2927 209
Size: 637 1104 977 776
Fragments arranged by size:
1104 977 776 637
AgeI A'CCGG_T
Cuts at: 1882 1882
Size: 3494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 272 304 272
Size: 32 3462
AlwNI CAG_nnn'CTG
Cuts at: 799 2366 799
Size: 1567 1927
ApaLI G'TGCA_C
Cuts at: 2264 2264
Size: 3494
ApoI r'AATT_y
Cuts at: 232 271 498 2847 3103 232
Size: 39 227 2349 256 623
Fragments arranged by size:
2349 623 256 227 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 338 514 338
Size: 176 3318
BamHI G'GATC_C
Cuts at: 519 519
Size: 3494
BanI G'GyrC_C
Cuts at: 510 924 1172 1207 510
Size: 414 248 35 2797
Fragments arranged by size:
2797 414 248 35
BanII G_rGCy'C
Cuts at: 508 1538 508
Size: 1030 2464
BbeI G_GCGC'C
Cuts at: 928 1176 928
Size: 248 3246
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2153 3494 1392
Size: 761 1341 1392
Fragments arranged by size:
1392 1341 761
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglII A'GATC_T
Cuts at: 342 417 1009 3046 342
Size: 75 592 2037 790
Fragments arranged by size:
2037 790 592 75
BlpI GC'TnA_GC
Cuts at: 606 606
Size: 3494
Bme1580I G_kGCm'C
Cuts at: 1119 1212 2268 1119
Size: 93 1056 2345
Fragments arranged by size:
2345 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 1122 3266 706
Size: 81 335 2144 934
Fragments arranged by size:
2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1549 2041 2339 670
Size: 879 492 298 1825
Fragments arranged by size:
1825 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 847 847
Size: 3494
BsaBI GATnn'nnATC
Cuts at: 6 191 1008 3045 6
Size: 185 817 2037 455
Fragments arranged by size:
2037 817 455 185
BsaHI Gr'CG_yC
Cuts at: 925 1173 1875 3371 925
Size: 248 702 1496 1048
Fragments arranged by size:
1496 1048 702 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2156 2303 2714 683
Size: 521 678 274 147 411 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 3494
BseYI C'CCAG_C
Cuts at: 919 1677 2254 2750 919
Size: 758 577 496 1663
Fragments arranged by size:
1663 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1036 1082 2290 104
Size: 932 46 1208 1308
Fragments arranged by size:
1308 1208 932 46
BsiHKAI G_wGCw'C
Cuts at: 508 1286 1476 2268 508
Size: 778 190 792 1734
Fragments arranged by size:
1734 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 508 1119 1212 1286 1476 1538 2268 508
Size: 611 93 74 190 62 730 1734
Fragments arranged by size:
1734 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2714 2714
Size: 3494
BspHI T'CATG_A
Cuts at: 180 2670 180
Size: 2490 1004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 758 1060 1441 544
Size: 214 302 381 2597
Fragments arranged by size:
2597 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1406 1406
Size: 3494
BsrFI r'CCGG_y
Cuts at: 1492 1673 1882 1492
Size: 181 209 3104
Fragments arranged by size:
3104 209 181
BssSI C'ACGA_G
Cuts at: 1765 2123 1765
Size: 358 3136
BstAPI GCAn_nnn'nTGC
Cuts at: 871 871
Size: 3494
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstYI r'GATC_y
Cuts at: 1 114 342 417 519 986 1009 1344 1590
Size: 113 228 75 102 467 23 335 246
Cuts at: 1590 2602 2688 2700 3046 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 228 113
102 86 75 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1444 1625 1788 1444
Size: 181 163 3150
Fragments arranged by size:
3150 181 163
BtsI GCAGTG_nn'
Cuts at: 737 737
Size: 3494
DraI TTT'AAA
Cuts at: 493 493
Size: 3494
DrdI GACnn_nn'nnGTC
Cuts at: 1200 2058 1200
Size: 858 2636
EaeI y'GGCC_r
Cuts at: 1079 1253 1644 1671 3016 1079
Size: 174 391 27 1345 1557
Fragments arranged by size:
1557 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1079 1079
Size: 3494
EarI CTCTTCn'nnn_
Cuts at: 245 299 308 411 1517 1727 245
Size: 54 9 103 1106 210 2012
Fragments arranged by size:
2012 1106 210 103 54 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2012 2158 3304 1702
Size: 310 146 1146 1892
Fragments arranged by size:
1892 1146 310 146
EcoICRI GAG'CTC
Cuts at: 506 506
Size: 3494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2498 2766 1319
Size: 432 747 268 2047
Fragments arranged by size:
2047 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 633 633
Size: 3494
EcoRI G'AATT_C
Cuts at: 232 498 232
Size: 266 3228
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1513
Size: 3494
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 928 1176 2198 3177 928
Size: 248 1022 979 1245
Fragments arranged by size:
1245 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1336 1366 1368 1398 1336
Size: 30 2 30 3432
Fragments arranged by size:
3432 30 30 2
HincII GTy'rAC
Cuts at: 533 533
Size: 3494
KasI G'GCGC_C
Cuts at: 924 1172 924
Size: 248 3246
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2349 2165
Size: 184 3310
MscI TGG'CCA
Cuts at: 1255 1255
Size: 3494
MslI CAynn'nnrTG
Cuts at: 179 185 214 1483 1610 179
Size: 6 29 1269 127 2063
Fragments arranged by size:
2063 1269 127 29 6
MspA1I CmG'CkG
Cuts at: 605 919 1279 2292 2537 605
Size: 314 360 1013 245 1562
Fragments arranged by size:
1562 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1675 1675
Size: 3494
NarI GG'CG_CC
Cuts at: 925 1173 925
Size: 248 3246
NgoMIV G'CCGG_C
Cuts at: 1673 1673
Size: 3494
NspI r_CATG'y
Cuts at: 213 547 1047 1954 213
Size: 334 500 907 1753
Fragments arranged by size:
1753 907 500 334
PciI A'CATG_T
Cuts at: 209 1950 209
Size: 1741 1753
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 3462
PstI C_TGCA'G
Cuts at: 541 1226 541
Size: 685 2809
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 919 1279 919
Size: 360 3134
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 508 508
Size: 3494
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 308 411 1517 1727 308
Size: 103 1106 210 2075
Fragments arranged by size:
2075 1106 210 103
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 132 3011 132
Size: 2879 615
SfcI C'TryA_G
Cuts at: 33 537 1222 2215 2406 33
Size: 504 685 993 191 1121
Fragments arranged by size:
1121 993 685 504 191
SfoI GGC'GCC
Cuts at: 926 1174 926
Size: 248 3246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SmaI CCC'GGG
Cuts at: 516 516
Size: 3494
SmlI C'TyrA_G
Cuts at: 338 649 1564 2056 2318 338
Size: 311 915 492 262 1514
Fragments arranged by size:
1514 915 492 311 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
SspI AAT'ATT
Cuts at: 486 486
Size: 3494
StyI C'CwwG_G
Cuts at: 628 982 628
Size: 354 3140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1210 1669 1878 1077
Size: 133 459 209 2693
Fragments arranged by size:
2693 459 209 133
TatI w'GTAC_w
Cuts at: 130 206 3009 130
Size: 76 2803 615
Fragments arranged by size:
2803 615 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 989 989
Size: 3494
XhoI C'TCGA_G
Cuts at: 338 338
Size: 3494
XmaI C'CCGG_G
Cuts at: 514 514
Size: 3494
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XmnI ZraI