Restriction map for
pF1KB8348
(Circular) (Six-base) MAPSORT of: pF1KB8348.seq Check: 6344 from: 1 to: 4550
pF1KB8348 4550 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AatII G_ACGT'C
Cuts at: 572 572
Size: 4550
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 794 842 977 2375 2807 3554 3822 704
Size: 90 48 135 1398 432 747 268 1432
Fragments arranged by size:
1432 1398 747 432 268 135 90 48
AfeI AGC'GCT
Cuts at: 1261 1261
Size: 4550
AflIII A'CryG_T
Cuts at: 255 1902 3006 3983 255
Size: 1647 1104 977 822
Fragments arranged by size:
1647 1104 977 822
AgeI A'CCGG_T
Cuts at: 889 2938 889
Size: 2049 2501
AlwNI CAG_nnn'CTG
Cuts at: 534 1855 3422 534
Size: 1321 1567 1662
Fragments arranged by size:
1662 1567 1321
ApaLI G'TGCA_C
Cuts at: 3320 3320
Size: 4550
ApoI r'AATT_y
Cuts at: 1467 1554 3903 4159 1467
Size: 87 2349 256 1858
Fragments arranged by size:
2349 1858 256 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 1570 1570
Size: 4550
BamHI G'GATC_C
Cuts at: 1134 1575 1134
Size: 441 4109
BanI G'GyrC_C
Cuts at: 1108 1401 1566 1980 2228 2263 1108
Size: 293 165 414 248 35 3395
Fragments arranged by size:
3395 414 293 248 165 35
BanII G_rGCy'C
Cuts at: 253 869 1067 1366 1564 2594 253
Size: 616 198 299 198 1030 2209
Fragments arranged by size:
2209 1030 616 299 198 198
BbeI G_GCGC'C
Cuts at: 1405 1984 2232 1405
Size: 579 248 3723
Fragments arranged by size:
3723 579 248
BbsI GAAGACnn'nnnn_
Cuts at: 965 965
Size: 4550
BbvCI CC'TCA_GC
Cuts at: 263 263
Size: 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 499 465
Size: 34 4516
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3209 4550 2448
Size: 761 1341 2448
Fragments arranged by size:
2448 1341 761
BclI T'GATC_A
Cuts at: 2070 2070
Size: 4550
BfrBI ATG'CAT
Cuts at: 765 765
Size: 4550
BglII A'GATC_T
Cuts at: 2065 4102 2065
Size: 2037 2513
BlpI GC'TnA_GC
Cuts at: 1201 1662 1201
Size: 461 4089
Bme1580I G_kGCm'C
Cuts at: 950 1537 2175 2268 3324 950
Size: 587 638 93 1056 2176
Fragments arranged by size:
2176 1056 638 587 93
BmrI ACTGGGnnnn_n'
Cuts at: 906 1487 1762 1843 2178 4322 906
Size: 581 275 81 335 2144 1134
Fragments arranged by size:
2144 1134 581 335 275 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 556 1071 1081 1288 1397 204
Size: 352 515 10 207 109 3357
Fragments arranged by size:
3357 515 352 207 109 10
Bpu10I CC'TnA_GC
Cuts at: 263 263
Size: 4550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 1726 2605 3097 3395 324
Size: 1402 879 492 298 1479
Fragments arranged by size:
1479 1402 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4254 4254
Size: 4550
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 983 2064 4101 6
Size: 977 1081 2037 455
Fragments arranged by size:
2037 1081 977 455
BsaHI Gr'CG_yC
Cuts at: 569 1402 1981 2229 2931 4427 569
Size: 833 579 248 702 1496 692
Fragments arranged by size:
1496 833 702 692 579 248
BsaWI w'CCGG_w
Cuts at: 889 1739 2260 2938 3212 3359 3770 889
Size: 850 521 678 274 147 411 1669
Fragments arranged by size:
1669 850 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1054 1084 1348 1378 1054
Size: 30 264 30 4226
Fragments arranged by size:
4226 264 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 448 692 207
Size: 241 244 4065
Fragments arranged by size:
4065 244 241
BseYI C'CCAG_C
Cuts at: 288 649 1157 1975 2733 3310 3806 288
Size: 361 508 818 758 577 496 1032
Fragments arranged by size:
1032 818 758 577 508 496 361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 806
Size: 4550
BsiEI CG_ry'CG
Cuts at: 104 2092 2138 3346 104
Size: 1988 46 1208 1308
Fragments arranged by size:
1988 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1067 1564 2342 2532 3324 1067
Size: 497 778 190 792 2293
Fragments arranged by size:
2293 792 778 497 190
BsmI GAATG_Cn'
Cuts at: 364 364
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 253 869 950 1067 1366 1537 1564 2175 2268
Size: 616 81 117 299 171 27 611 93
Cuts at: 2268 2342 2532 2594 3324 253
Size: 74 190 62 730 1479
Fragments arranged by size:
1479 730 616 611 299 190 171 117
93 81 74 62 27
BspEI T'CCGG_A
Cuts at: 3770 3770
Size: 4550
BspHI T'CATG_A
Cuts at: 1238 3726 1238
Size: 2488 2062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1600 1814 2116 2497 1600
Size: 214 302 381 3653
Fragments arranged by size:
3653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1167 2842 2896 4380 4545 50
Size: 1117 1675 54 1484 165 55
Fragments arranged by size:
1675 1484 1117 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2462 2462
Size: 4550
BsrFI r'CCGG_y
Cuts at: 889 2548 2729 2938 889
Size: 1659 181 209 2501
Fragments arranged by size:
2501 1659 209 181
BsrGI T'GTAC_A
Cuts at: 1331 1331
Size: 4550
BssSI C'ACGA_G
Cuts at: 2821 3179 2821
Size: 358 4192
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstEII G'GTnAC_C
Cuts at: 297 297
Size: 4550
BstXI CCAn_nnnn'nTGG
Cuts at: 309 334 309
Size: 25 4525
BstYI r'GATC_y
Cuts at: 1 495 978 1037 1134 1575 2042 2065 2400
Size: 494 483 59 97 441 467 23 335
Cuts at: 2400 2646 3658 3744 3756 4102 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 494 483 467 449 441 346 335
246 97 86 59 23 12
BtgI C'CryG_G
Cuts at: 107 754 1251 1284 107
Size: 647 497 33 3373
Fragments arranged by size:
3373 647 497 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 369 535 1035 1793 369
Size: 166 500 758 3126
Fragments arranged by size:
3126 758 500 166
ClaI AT'CG_AT
Cuts at: 596 596
Size: 4550
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3114 2256
Size: 858 3692
EaeI y'GGCC_r
Cuts at: 110 368 897 1032 2135 2309 2700 2727 4072
Size: 258 529 135 1103 174 391 27 1345
Cuts at: 4072 110
Size: 588
Fragments arranged by size:
1345 1103 588 529 391 258 174 135
27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 710 1165 2573 2783 710
Size: 455 1408 210 2477
Fragments arranged by size:
2477 1408 455 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2758 3068 3214 4360 2758
Size: 310 146 1146 2948
Fragments arranged by size:
2948 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1065 1562 1065
Size: 497 4053
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 556 704 794 842 977 1071 1081 1288
Size: 352 148 90 48 135 94 10 207
Cuts at: 1288 1397 2375 2807 3554 3822 204
Size: 109 978 432 747 268 932
Fragments arranged by size:
978 932 747 432 352 268 207 148
135 109 94 90 48 10
EcoO109I rG'GnC_Cy
Cuts at: 1299 1689 1299
Size: 390 4160
EcoRI G'AATT_C
Cuts at: 1467 1554 1467
Size: 87 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 147 648 1296 1410 2569 147
Size: 501 648 114 1159 2128
Fragments arranged by size:
2128 1159 648 501 114
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 1158 1263 1405 1984 2232 3254 4233 1158
Size: 105 142 579 248 1022 979 1475
Fragments arranged by size:
1475 1022 979 579 248 142 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 262 294 1054 1086 1119 1151 2392
Size: 32 91 32 760 32 33 32 1241
Cuts at: 2392 2422 2424 2454 139
Size: 30 2 30 2235
Fragments arranged by size:
2235 1241 760 91 33 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 573 1589 573
Size: 1016 3534
KasI G'GCGC_C
Cuts at: 1401 1980 2228 1401
Size: 579 248 3723
Fragments arranged by size:
3723 579 248
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 255 3983 255
Size: 3728 822
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1106 3221 3405 1052
Size: 54 2115 184 2197
Fragments arranged by size:
2197 2115 184 54
MscI TGG'CCA
Cuts at: 112 370 1034 2311 112
Size: 258 664 1277 2351
Fragments arranged by size:
2351 1277 664 258
MslI CAynn'nnrTG
Cuts at: 307 2539 2666 307
Size: 2232 127 2191
Fragments arranged by size:
2232 2191 127
MspA1I CmG'CkG
Cuts at: 1091 1661 1975 2335 3348 3593 1091
Size: 570 314 360 1013 245 2048
Fragments arranged by size:
2048 1013 570 360 314 245
NaeI GCC'GGC
Cuts at: 2731 2731
Size: 4550
NarI GG'CG_CC
Cuts at: 1402 1981 2229 1402
Size: 579 248 3723
Fragments arranged by size:
3723 579 248
NcoI C'CATG_G
Cuts at: 107 1251 107
Size: 1144 3406
NdeI CA'TA_TG
Cuts at: 861 1517 861
Size: 656 3894
NgoMIV G'CCGG_C
Cuts at: 2729 2729
Size: 4550
NsiI A_TGCA'T
Cuts at: 767 767
Size: 4550
NspI r_CATG'y
Cuts at: 447 769 1603 2103 3010 447
Size: 322 834 500 907 1987
Fragments arranged by size:
1987 907 834 500 322
PciI A'CATG_T
Cuts at: 3006 3006
Size: 4550
PflMI CCAn_nnn'nTGG
Cuts at: 1030 1030
Size: 4550
PfoI T'CCnGG_A
Cuts at: 1058 1058
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4518
PshAI GACnn'nnGTC
Cuts at: 965 1246 965
Size: 281 4269
PstI C_TGCA'G
Cuts at: 1597 2282 1597
Size: 685 3865
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4550
PvuII CAG'CTG
Cuts at: 1091 1975 2335 1091
Size: 884 360 3306
Fragments arranged by size:
3306 884 360
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 1067 1564 1067
Size: 497 4053
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 1165 2573 2783 1165
Size: 1408 210 2932
Fragments arranged by size:
2932 1408 210
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 1593 2278 3271 3462 33
Size: 1560 685 993 191 1121
Fragments arranged by size:
1560 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1403 1982 2230 1403
Size: 579 248 3723
Fragments arranged by size:
3723 579 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4550
SmlI C'TyrA_G
Cuts at: 339 1705 2620 3112 3374 339
Size: 1366 915 492 262 1515
Fragments arranged by size:
1515 1366 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 769 1603 2103 769
Size: 834 500 3216
Fragments arranged by size:
3216 834 500
StyI C'CwwG_G
Cuts at: 107 623 1244 1251 1684 2038 107
Size: 516 621 7 433 354 2619
Fragments arranged by size:
2619 621 516 433 354 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1394 2133 2266 2725 2934 1394
Size: 739 133 459 209 3010
Fragments arranged by size:
3010 739 459 209 133
TatI w'GTAC_w
Cuts at: 511 1331 4065 511
Size: 820 2734 996
Fragments arranged by size:
2734 996 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 4518
Tth111I GACn'n_nGTC
Cuts at: 210 2347 210
Size: 2137 2413
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 595 1460 2045 595
Size: 865 585 3100
Fragments arranged by size:
3100 865 585
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4550
ZraI GAC'GTC
Cuts at: 570 570
Size: 4550
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BsiWI BsmBI BssHII
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI