(Circular) (Six-base) MAPSORT of: pF1KB8352.seq Check: 1260 from: 1 to: 5192 pF1KB8352 5192 bp With 182 enzymes: SgfI * July 2, 2008 13:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 461 461 Size: 5192 AccI GT'mk_AC Cuts at: 2230 2230 Size: 5192 Acc65I G'GTAC_C Cuts at: 2208 2208 Size: 5192 AclI AA'CG_TT Cuts at: 5032 5032 Size: 5192 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 928 1392 3017 3449 4196 4464 928 Size: 464 1625 432 747 268 1656 Fragments arranged by size: 1656 1625 747 464 432 268 AfeI AGC'GCT Cuts at: 367 367 Size: 5192 AflIII A'CryG_T Cuts at: 1691 2544 3648 4625 1691 Size: 853 1104 977 2258 Fragments arranged by size: 2258 1104 977 853 AgeI A'CCGG_T Cuts at: 3580 3580 Size: 5192 AleI CACnn'nnGTG Cuts at: 1972 1972 Size: 5192 AlwNI CAG_nnn'CTG Cuts at: 652 1131 1740 2067 2109 2497 4064 652 Size: 479 609 327 42 388 1567 1780 Fragments arranged by size: 1780 1567 609 479 388 327 42 ApaI G_GGCC'C Cuts at: 127 2091 2162 127 Size: 1964 71 3157 Fragments arranged by size: 3157 1964 71 ApaLI G'TGCA_C Cuts at: 436 3962 436 Size: 3526 1666 ApoI r'AATT_y Cuts at: 2176 2196 4545 4801 2176 Size: 20 2349 256 2567 Fragments arranged by size: 2567 2349 256 20 AseI AT'TA_AT Cuts at: 20 20 Size: 5192 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5192 AvaI C'yCGr_G Cuts at: 126 531 803 1154 1360 2212 126 Size: 405 272 351 206 852 3106 Fragments arranged by size: 3106 852 405 351 272 206 AvrII C'CTAG_G Cuts at: 1824 1824 Size: 5192 BamHI G'GATC_C Cuts at: 1957 2217 1957 Size: 260 4932 BanI G'GyrC_C Cuts at: 148 171 233 291 491 520 808 1342 1468 Size: 23 62 58 200 29 288 534 126 Cuts at: 1468 2208 2622 2870 2905 148 Size: 740 414 248 35 2435 Fragments arranged by size: 2435 740 534 414 288 248 200 126 62 58 35 29 23 BanII G_rGCy'C Cuts at: 127 137 224 287 580 659 742 765 833 Size: 10 87 63 293 79 83 23 68 Cuts at: 833 1713 2091 2162 2206 3236 127 Size: 880 378 71 44 1030 2083 Fragments arranged by size: 2083 1030 880 378 293 87 83 79 71 68 63 44 23 10 BbeI G_GCGC'C Cuts at: 152 295 2626 2874 152 Size: 143 2331 248 2470 Fragments arranged by size: 2470 2331 248 143 BbsI GAAGACnn'nnnn_ Cuts at: 1465 1465 Size: 5192 BbvCI CC'TCA_GC Cuts at: 597 1124 1511 597 Size: 527 387 4278 Fragments arranged by size: 4278 527 387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1916 1950 1991 2025 1916 Size: 34 41 34 5083 Fragments arranged by size: 5083 41 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3090 3851 5192 3090 Size: 761 1341 3090 Fragments arranged by size: 3090 1341 761 BclI T'GATC_A Cuts at: 2712 2712 Size: 5192 BglI GCCn_nnn'nGGC Cuts at: 424 1829 424 Size: 1405 3787 BglII A'GATC_T Cuts at: 1601 2707 4744 1601 Size: 1106 2037 2049 Fragments arranged by size: 2049 2037 1106 BlpI GC'TnA_GC Cuts at: 2304 2304 Size: 5192 Bme1580I G_kGCm'C Cuts at: 127 440 811 1345 1767 2091 2162 2817 2910 Size: 313 371 534 422 324 71 655 93 Cuts at: 2910 3966 127 Size: 1056 1353 Fragments arranged by size: 1353 1056 655 534 422 371 324 313 93 71 BmrI ACTGGGnnnn_n' Cuts at: 2404 2485 2820 4964 2404 Size: 81 335 2144 2632 Fragments arranged by size: 2632 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3559 3559 Size: 5192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 238 594 689 946 1093 1541 238 Size: 356 95 257 147 448 3889 Fragments arranged by size: 3889 448 356 257 147 95 Bpu10I CC'TnA_GC Cuts at: 597 653 1124 1511 597 Size: 56 471 387 4278 Fragments arranged by size: 4278 471 387 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 817 2368 3247 3739 4037 817 Size: 1551 879 492 298 1972 Fragments arranged by size: 1972 1551 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1426 4896 1426 Size: 3470 1722 BsaAI yAC'GTr Cuts at: 1692 2545 1692 Size: 853 4339 BsaBI GATnn'nnATC Cuts at: 6 2032 2706 4743 6 Size: 2026 674 2037 455 Fragments arranged by size: 2037 2026 674 455 BsaHI Gr'CG_yC Cuts at: 149 212 292 298 305 2623 2871 3573 5069 Size: 63 80 6 7 2318 248 702 1496 Cuts at: 5069 149 Size: 272 Fragments arranged by size: 2318 1496 702 272 248 80 63 7 6 BsaWI w'CCGG_w Cuts at: 2381 2902 3580 3854 4001 4412 2381 Size: 521 678 274 147 411 3161 Fragments arranged by size: 3161 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 958 988 958 Size: 30 5162 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 841 1378 1519 1528 841 Size: 537 141 9 4505 Fragments arranged by size: 4505 537 141 9 BseYI C'CCAG_C Cuts at: 152 538 618 1186 1251 1300 1884 1940 2617 Size: 386 80 568 65 49 584 56 677 Cuts at: 2617 3375 3952 4448 152 Size: 758 577 496 896 Fragments arranged by size: 896 758 677 584 577 568 496 386 80 65 56 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 536 1627 536 Size: 1091 4101 BsiEI CG_ry'CG Cuts at: 104 2734 2780 3988 104 Size: 2630 46 1208 1308 Fragments arranged by size: 2630 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 440 659 742 833 1023 1549 2206 2984 3174 Size: 219 83 91 190 526 657 778 190 Cuts at: 3174 3966 440 Size: 792 1666 Fragments arranged by size: 1666 792 778 657 526 219 190 190 91 83 BsmBI CGTCTCn'nnnn_ Cuts at: 1087 1087 Size: 5192 Bsp1286I G_dGCh'C Cuts at: 127 137 224 287 440 580 659 742 765 Size: 10 87 63 153 140 79 83 23 Cuts at: 765 811 833 1023 1345 1549 1713 1767 2091 Size: 46 22 190 322 204 164 54 324 Cuts at: 2091 2162 2206 2817 2910 2984 3174 3236 3966 Size: 71 44 611 93 74 190 62 730 Cuts at: 3966 127 Size: 1353 Fragments arranged by size: 1353 730 611 324 322 204 190 190 164 153 140 93 87 83 79 74 71 63 62 54 46 44 23 22 10 BspEI T'CCGG_A Cuts at: 4412 4412 Size: 5192 BspHI T'CATG_A Cuts at: 4368 4368 Size: 5192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 461 1364 1549 2242 2456 2758 3139 461 Size: 903 185 693 214 302 381 2514 Fragments arranged by size: 2514 903 693 381 302 214 185 BsrBI CCG'CTC Cuts at: 50 3484 3538 5022 5187 50 Size: 3434 54 1484 165 55 Fragments arranged by size: 3434 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3104 3104 Size: 5192 BsrFI r'CCGG_y Cuts at: 1903 3190 3371 3580 1903 Size: 1287 181 209 3515 Fragments arranged by size: 3515 1287 209 181 BssHII G'CGCG_C Cuts at: 277 277 Size: 5192 BssSI C'ACGA_G Cuts at: 433 3463 3821 433 Size: 3030 358 1804 Fragments arranged by size: 3030 1804 358 BstAPI GCAn_nnn'nTGC Cuts at: 2569 2569 Size: 5192 BstBI TT'CG_AA Cuts at: 3553 3553 Size: 5192 BstXI CCAn_nnnn'nTGG Cuts at: 244 1974 244 Size: 1730 3462 BstYI r'GATC_y Cuts at: 1 1601 1957 2217 2684 2707 3042 3288 4300 Size: 1600 356 260 467 23 335 246 1012 Cuts at: 4300 4386 4398 4744 1 Size: 86 12 346 449 Fragments arranged by size: 1600 1012 467 449 356 346 335 260 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1894 1913 1894 Size: 19 5173 BtgI C'CryG_G Cuts at: 107 1197 1729 1805 1973 107 Size: 1090 532 76 168 3326 Fragments arranged by size: 3326 1090 532 168 76 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1575 3142 3323 3486 1575 Size: 1567 181 163 3281 Fragments arranged by size: 3281 1567 181 163 BtsI GCAGTG_nn' Cuts at: 524 944 1086 1549 2435 524 Size: 420 142 463 886 3281 Fragments arranged by size: 3281 886 463 420 142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 980 1015 980 Size: 35 5157 DraI TTT'AAA Cuts at: 2191 2191 Size: 5192 DrdI GACnn_nn'nnGTC Cuts at: 2898 3756 2898 Size: 858 4334 EaeI y'GGCC_r Cuts at: 110 383 425 2777 2951 3342 3369 4714 110 Size: 273 42 2352 174 391 27 1345 588 Fragments arranged by size: 2352 1345 588 391 273 174 42 27 EagI C'GGCC_G Cuts at: 2777 2777 Size: 5192 EarI CTCTTCn'nnn_ Cuts at: 895 1496 3215 3425 895 Size: 601 1719 210 2662 Fragments arranged by size: 2662 1719 601 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3400 3710 3856 5002 3400 Size: 310 146 1146 3590 Fragments arranged by size: 3590 1146 310 146 EcoICRI GAG'CTC Cuts at: 657 740 831 2204 657 Size: 83 91 1373 3645 Fragments arranged by size: 3645 1373 91 83 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 238 594 689 928 946 1093 1392 1541 3017 Size: 356 95 239 18 147 299 149 1476 Cuts at: 3017 3449 4196 4464 238 Size: 432 747 268 966 Fragments arranged by size: 1476 966 747 432 356 299 268 239 149 147 95 18 EcoNI CCTnn'n_nnAGG Cuts at: 754 958 1360 1897 2077 2143 754 Size: 204 402 537 180 66 3803 Fragments arranged by size: 3803 537 402 204 180 66 EcoO109I rG'GnC_Cy Cuts at: 649 1792 2087 2088 2158 2331 649 Size: 1143 295 1 70 173 3510 Fragments arranged by size: 3510 1143 295 173 70 1 EcoRI G'AATT_C Cuts at: 2196 2196 Size: 5192 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 695 966 3211 695 Size: 271 2245 2676 Fragments arranged by size: 2676 2245 271 FspI TGC'GCA Cuts at: 2973 2973 Size: 5192 HaeII r_GCGC'y Cuts at: 152 295 369 1750 2626 2874 3896 4875 152 Size: 143 74 1381 876 248 1022 979 469 Fragments arranged by size: 1381 1022 979 876 469 248 143 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 958 990 1121 1153 1467 1499 3034 3064 3066 Size: 32 131 32 314 32 1535 30 2 Cuts at: 3066 3096 958 Size: 30 3054 Fragments arranged by size: 3054 1535 314 131 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2231 2231 Size: 5192 KasI G'GCGC_C Cuts at: 148 291 2622 2870 148 Size: 143 2331 248 2470 Fragments arranged by size: 2470 2331 248 143 KpnI G_GTAC'C Cuts at: 2212 2212 Size: 5192 MluI A'CGCG_T Cuts at: 4625 4625 Size: 5192 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1241 3863 4047 1241 Size: 2622 184 2386 Fragments arranged by size: 2622 2386 184 MscI TGG'CCA Cuts at: 2953 2953 Size: 5192 MslI CAynn'nnrTG Cuts at: 1594 1972 3181 3308 1594 Size: 378 1209 127 3478 Fragments arranged by size: 3478 1209 378 127 MspA1I CmG'CkG Cuts at: 459 972 1626 1782 2106 2303 2617 2977 3990 Size: 513 654 156 324 197 314 360 1013 Cuts at: 3990 4235 459 Size: 245 1416 Fragments arranged by size: 1416 1013 654 513 360 324 314 245 197 156 NaeI GCC'GGC Cuts at: 1905 3373 1905 Size: 1468 3724 NarI GG'CG_CC Cuts at: 149 292 2623 2871 149 Size: 143 2331 248 2470 Fragments arranged by size: 2470 2331 248 143 NcoI C'CATG_G Cuts at: 107 1197 1729 107 Size: 1090 532 3570 Fragments arranged by size: 3570 1090 532 NgoMIV G'CCGG_C Cuts at: 1903 3371 1903 Size: 1468 3724 NspI r_CATG'y Cuts at: 2245 2745 3652 2245 Size: 500 907 3785 Fragments arranged by size: 3785 907 500 PasI CC'CwG_GG Cuts at: 179 179 Size: 5192 PciI A'CATG_T Cuts at: 3648 3648 Size: 5192 PflMI CCAn_nnn'nTGG Cuts at: 1806 2067 1806 Size: 261 4931 PmeI GTTT'AAAC Cuts at: 2191 2191 Size: 5192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 4331 Size: 32 5160 PspOMI G'GGCC_C Cuts at: 123 2087 2158 123 Size: 1964 71 3157 Fragments arranged by size: 3157 1964 71 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1018 1050 1018 Size: 32 5160 PstI C_TGCA'G Cuts at: 458 758 939 1572 1787 2105 2239 2924 458 Size: 300 181 633 215 318 134 685 2726 Fragments arranged by size: 2726 685 633 318 300 215 181 134 PvuI CG_AT'CG Cuts at: 104 104 Size: 5192 PvuII CAG'CTG Cuts at: 459 972 1626 1782 2106 2617 2977 459 Size: 513 654 156 324 511 360 2674 Fragments arranged by size: 2674 654 513 511 360 324 156 RsrII CG'GwC_CG Cuts at: 3387 3387 Size: 5192 SacI G_AGCT'C Cuts at: 659 742 833 2206 659 Size: 83 91 1373 3645 Fragments arranged by size: 3645 1373 91 83 SalI G'TCGA_C Cuts at: 2229 2229 Size: 5192 SapI GCTCTTCn'nnn_ Cuts at: 3215 3425 3215 Size: 210 4982 SbfI CC_TGCA'GG Cuts at: 758 2239 758 Size: 1481 3711 ScaI AGT'ACT Cuts at: 4709 4709 Size: 5192 SexAI A'CCwGG_T Cuts at: 1493 1493 Size: 5192 SfcI C'TryA_G Cuts at: 33 454 754 935 1568 1783 2101 2235 2920 Size: 421 300 181 633 215 318 134 685 Cuts at: 2920 3913 4104 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 685 633 421 318 300 215 191 181 134 SfoI GGC'GCC Cuts at: 150 293 2624 2872 150 Size: 143 2331 248 2470 Fragments arranged by size: 2470 2331 248 143 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5192 SmaI CCC'GGG Cuts at: 128 805 1156 2214 128 Size: 677 351 1058 3106 Fragments arranged by size: 3106 1058 677 351 SmlI C'TyrA_G Cuts at: 832 2347 3262 3754 4016 832 Size: 1515 915 492 262 2008 Fragments arranged by size: 2008 1515 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5192 SphI G_CATG'C Cuts at: 2245 2745 2245 Size: 500 4692 SrfI GCCC'GGGC Cuts at: 1156 1156 Size: 5192 StuI AGG'CCT Cuts at: 571 1102 1665 571 Size: 531 563 4098 Fragments arranged by size: 4098 563 531 StyI C'CwwG_G Cuts at: 107 1197 1436 1729 1824 1989 2326 2680 107 Size: 1090 239 293 95 165 337 354 2619 Fragments arranged by size: 2619 1090 354 337 293 239 165 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 236 361 826 845 973 1004 1260 1555 2775 Size: 125 465 19 128 31 256 295 1220 Cuts at: 2775 2908 3367 3576 236 Size: 133 459 209 1852 Fragments arranged by size: 1852 1220 465 459 295 256 209 133 128 125 31 19 TatI w'GTAC_w Cuts at: 1367 4707 1367 Size: 3340 1852 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4709 4741 4709 Size: 32 5160 Tth111I GACn'n_nGTC Cuts at: 2989 2989 Size: 5192 XbaI T'CTAG_A Cuts at: 2223 2223 Size: 5192 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1068 1115 1224 1803 2687 1068 Size: 47 109 579 884 3573 Fragments arranged by size: 3573 884 579 109 47 XmaI C'CCGG_G Cuts at: 126 803 1154 2212 126 Size: 677 351 1058 3106 Fragments arranged by size: 3106 1058 677 351 XmnI GAAnn'nnTTC Cuts at: 1030 1030 Size: 5192 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AhdI AlfI AloI AscI BaeI BfrBI BmgBI BmtI BsiWI BsmI BsrGI BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI SacII SanDI SfiI SgrAI SnaBI SspI SwaI XhoI ZraI