Restriction map for
pF1KB8357
(Circular) (Six-base) MAPSORT of: pF1KB8357.seq Check: 7565 from: 1 to: 3902
pF1KB8357 3902 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 155 298 155
Size: 143 3759
AccI GT'mk_AC
Cuts at: 161 940 161
Size: 779 3123
Acc65I G'GTAC_C
Cuts at: 918 918
Size: 3902
AclI AA'CG_TT
Cuts at: 3742 3742
Size: 3902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1727 2159 2906 3174 1727
Size: 432 747 268 2455
Fragments arranged by size:
2455 747 432 268
AflIII A'CryG_T
Cuts at: 1254 2358 3335 1254
Size: 1104 977 1821
Fragments arranged by size:
1821 1104 977
AgeI A'CCGG_T
Cuts at: 2290 2290
Size: 3902
AhdI GACnn_n'nnGTC
Cuts at: 294 294
Size: 3902
AleI CACnn'nnGTG
Cuts at: 346 346
Size: 3902
AlwNI CAG_nnn'CTG
Cuts at: 1207 2774 1207
Size: 1567 2335
ApaLI G'TGCA_C
Cuts at: 2672 2672
Size: 3902
ApoI r'AATT_y
Cuts at: 508 906 3255 3511 508
Size: 398 2349 256 899
Fragments arranged by size:
2349 899 398 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3902
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
AvaI C'yCGr_G
Cuts at: 632 922 632
Size: 290 3612
BamHI G'GATC_C
Cuts at: 739 927 739
Size: 188 3714
BanI G'GyrC_C
Cuts at: 918 1332 1580 1615 918
Size: 414 248 35 3205
Fragments arranged by size:
3205 414 248 35
BanII G_rGCy'C
Cuts at: 308 916 1946 308
Size: 608 1030 2264
Fragments arranged by size:
2264 1030 608
BbeI G_GCGC'C
Cuts at: 1336 1584 1336
Size: 248 3654
BbvCI CC'TCA_GC
Cuts at: 244 244
Size: 3902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 360 394 360
Size: 34 3868
BciVI GTATCCnnnnn_n'
Cuts at: 1800 2561 3902 1800
Size: 761 1341 1800
Fragments arranged by size:
1800 1341 761
BclI T'GATC_A
Cuts at: 1422 1422
Size: 3902
BglII A'GATC_T
Cuts at: 1417 3454 1417
Size: 2037 1865
BlpI GC'TnA_GC
Cuts at: 661 1014 661
Size: 353 3549
Bme1580I G_kGCm'C
Cuts at: 1527 1620 2676 1527
Size: 93 1056 2753
Fragments arranged by size:
2753 1056 93
BmgBI CAC'GTC
Cuts at: 159 159
Size: 3902
BmrI ACTGGGnnnn_n'
Cuts at: 761 1114 1195 1530 3674 761
Size: 353 81 335 2144 989
Fragments arranged by size:
2144 989 353 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 3902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 3902
Bpu10I CC'TnA_GC
Cuts at: 244 314 244
Size: 70 3832
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1957 2449 2747 1078
Size: 879 492 298 2233
Fragments arranged by size:
2233 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3606 3606
Size: 3902
BsaAI yAC'GTr
Cuts at: 1255 1255
Size: 3902
BsaBI GATnn'nnATC
Cuts at: 6 388 1416 3453 6
Size: 382 1028 2037 455
Fragments arranged by size:
2037 1028 455 382
BsaHI Gr'CG_yC
Cuts at: 152 295 1333 1581 2283 3779 152
Size: 143 1038 248 702 1496 275
Fragments arranged by size:
1496 1038 702 275 248 143
BsaWI w'CCGG_w
Cuts at: 1091 1612 2290 2564 2711 3122 1091
Size: 521 678 274 147 411 1871
Fragments arranged by size:
1871 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 106
Size: 30 3872
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 127
Size: 3902
BseYI C'CCAG_C
Cuts at: 622 869 884 1327 2085 2662 3158 622
Size: 247 15 443 758 577 496 1366
Fragments arranged by size:
1366 758 577 496 443 247 15
BsiEI CG_ry'CG
Cuts at: 104 1444 1490 2698 104
Size: 1340 46 1208 1308
Fragments arranged by size:
1340 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 308 783 916 1694 1884 2676 137
Size: 171 475 133 778 190 792 1363
Fragments arranged by size:
1363 792 778 475 190 171 133
Bsp1286I G_dGCh'C
Cuts at: 137 308 783 916 1527 1620 1694 1884 1946
Size: 171 475 133 611 93 74 190 62
Cuts at: 1946 2676 137
Size: 730 1363
Fragments arranged by size:
1363 730 611 475 190 171 133 93
74 62
BspEI T'CCGG_A
Cuts at: 3122 3122
Size: 3902
BspHI T'CATG_A
Cuts at: 3078 3078
Size: 3902
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 952 1166 1468 1849 362
Size: 590 214 302 381 2415
Fragments arranged by size:
2415 590 381 302 214
BsrBI CCG'CTC
Cuts at: 50 119 2194 2248 3732 3897 50
Size: 69 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 69 55 54
BsrDI GCAATG_nn'
Cuts at: 469 1814 469
Size: 1345 2557
BsrFI r'CCGG_y
Cuts at: 239 1900 2081 2290 239
Size: 1661 181 209 1851
Fragments arranged by size:
1851 1661 209 181
BssSI C'ACGA_G
Cuts at: 2173 2531 2173
Size: 358 3544
BstAPI GCAn_nnn'nTGC
Cuts at: 1279 1279
Size: 3902
BstBI TT'CG_AA
Cuts at: 2263 2263
Size: 3902
BstYI r'GATC_y
Cuts at: 1 739 927 1394 1417 1752 1998 3010 3096
Size: 738 188 467 23 335 246 1012 86
Cuts at: 3096 3108 3454 1
Size: 12 346 449
Fragments arranged by size:
1012 738 467 449 346 335 246 188
86 23 12
BtgI C'CryG_G
Cuts at: 107 175 189 107
Size: 68 14 3820
Fragments arranged by size:
3820 68 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 576 1852 2033 2196 576
Size: 1276 181 163 2282
Fragments arranged by size:
2282 1276 181 163
BtsI GCAGTG_nn'
Cuts at: 251 508 812 1145 251
Size: 257 304 333 3008
Fragments arranged by size:
3008 333 304 257
DraI TTT'AAA
Cuts at: 901 901
Size: 3902
DrdI GACnn_nn'nnGTC
Cuts at: 157 1608 2466 157
Size: 1451 858 1593
Fragments arranged by size:
1593 1451 858
EaeI y'GGCC_r
Cuts at: 237 793 1487 1661 2052 2079 3424 237
Size: 556 694 174 391 27 1345 715
Fragments arranged by size:
1345 715 694 556 391 174 27
EagI C'GGCC_G
Cuts at: 1487 1487
Size: 3902
EarI CTCTTCn'nnn_
Cuts at: 1925 2135 1925
Size: 210 3692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2110 2420 2566 3712 2110
Size: 310 146 1146 2300
Fragments arranged by size:
2300 1146 310 146
EcoICRI GAG'CTC
Cuts at: 306 914 306
Size: 608 3294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1727 2159 2906 3174 304
Size: 1423 432 747 268 1032
Fragments arranged by size:
1423 1032 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 680 680
Size: 3902
EcoO109I rG'GnC_Cy
Cuts at: 318 541 1041 318
Size: 223 500 3179
Fragments arranged by size:
3179 500 223
EcoRI G'AATT_C
Cuts at: 906 906
Size: 3902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1921 1921
Size: 3902
FspI TGC'GCA
Cuts at: 1683 1683
Size: 3902
HaeII r_GCGC'y
Cuts at: 1336 1584 2606 3585 1336
Size: 248 1022 979 1653
Fragments arranged by size:
1653 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 280 312 731 763 1744 1774 1776
Size: 32 37 32 419 32 981 30 2
Cuts at: 1776 1806 211
Size: 30 2307
Fragments arranged by size:
2307 981 419 37 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 941 941
Size: 3902
KasI G'GCGC_C
Cuts at: 1332 1580 1332
Size: 248 3654
KpnI G_GTAC'C
Cuts at: 922 922
Size: 3902
MluI A'CGCG_T
Cuts at: 3335 3335
Size: 3902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2573 2757 2573
Size: 184 3718
MscI TGG'CCA
Cuts at: 795 1663 795
Size: 868 3034
MslI CAynn'nnrTG
Cuts at: 346 1891 2018 346
Size: 1545 127 2230
Fragments arranged by size:
2230 1545 127
MspA1I CmG'CkG
Cuts at: 177 216 346 622 1013 1327 1687 2700 2945
Size: 39 130 276 391 314 360 1013 245
Cuts at: 2945 177
Size: 1134
Fragments arranged by size:
1134 1013 391 360 314 276 245 130
39
NaeI GCC'GGC
Cuts at: 241 2083 241
Size: 1842 2060
NarI GG'CG_CC
Cuts at: 1333 1581 1333
Size: 248 3654
NcoI C'CATG_G
Cuts at: 107 107
Size: 3902
NgoMIV G'CCGG_C
Cuts at: 239 2081 239
Size: 1842 2060
NspI r_CATG'y
Cuts at: 955 1455 2362 955
Size: 500 907 2495
Fragments arranged by size:
2495 907 500
PasI CC'CwG_GG
Cuts at: 682 715 682
Size: 33 3869
PciI A'CATG_T
Cuts at: 2358 2358
Size: 3902
PmeI GTTT'AAAC
Cuts at: 901 901
Size: 3902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 3041
Size: 32 3870
PstI C_TGCA'G
Cuts at: 949 1634 949
Size: 685 3217
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3902
PvuII CAG'CTG
Cuts at: 216 346 622 1327 1687 216
Size: 130 276 705 360 2431
Fragments arranged by size:
2431 705 360 276 130
RsrII CG'GwC_CG
Cuts at: 2097 2097
Size: 3902
SacI G_AGCT'C
Cuts at: 308 916 308
Size: 608 3294
SacII CC_GC'GG
Cuts at: 178 178
Size: 3902
SalI G'TCGA_C
Cuts at: 939 939
Size: 3902
SapI GCTCTTCn'nnn_
Cuts at: 1925 2135 1925
Size: 210 3692
SbfI CC_TGCA'GG
Cuts at: 949 949
Size: 3902
ScaI AGT'ACT
Cuts at: 3419 3419
Size: 3902
SfcI C'TryA_G
Cuts at: 33 195 845 945 1630 2623 2814 33
Size: 162 650 100 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 191 162 100
SfoI GGC'GCC
Cuts at: 1334 1582 1334
Size: 248 3654
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3902
SmaI CCC'GGG
Cuts at: 924 924
Size: 3902
SmlI C'TyrA_G
Cuts at: 1057 1972 2464 2726 1057
Size: 915 492 262 2233
Fragments arranged by size:
2233 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3902
SphI G_CATG'C
Cuts at: 955 1455 955
Size: 500 3402
StyI C'CwwG_G
Cuts at: 107 537 1036 1390 107
Size: 430 499 354 2619
Fragments arranged by size:
2619 499 430 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 455 480 1485 1618 2077 2286 455
Size: 25 1005 133 459 209 2071
Fragments arranged by size:
2071 1005 459 209 133 25
TatI w'GTAC_w
Cuts at: 3417 3417
Size: 3902
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3419 3451 3419
Size: 32 3870
Tth111I GACn'n_nGTC
Cuts at: 1699 1699
Size: 3902
XbaI T'CTAG_A
Cuts at: 933 933
Size: 3902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 881 1397 881
Size: 516 3386
XmaI C'CCGG_G
Cuts at: 922 922
Size: 3902
ZraI GAC'GTC
Cuts at: 153 296 153
Size: 143 3759
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BfrBI BglI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI