Restriction map for
pF1KB8362
(Circular) (Six-base) MAPSORT of: pF1KB8362.seq Check: 7399 from: 1 to: 4442
pF1KB8362 4442 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 469 590 1054 469
Size: 121 464 3857
Fragments arranged by size:
3857 464 121
AccI GT'mk_AC
Cuts at: 503 748 1480 503
Size: 245 732 3465
Fragments arranged by size:
3465 732 245
Acc65I G'GTAC_C
Cuts at: 329 1458 329
Size: 1129 3313
AclI AA'CG_TT
Cuts at: 4282 4282
Size: 4442
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1317 2267 2699 3446 3714 1317
Size: 950 432 747 268 2045
Fragments arranged by size:
2045 950 747 432 268
AflIII A'CryG_T
Cuts at: 1304 1794 2898 3875 1304
Size: 490 1104 977 1871
Fragments arranged by size:
1871 1104 977 490
AgeI A'CCGG_T
Cuts at: 2830 2830
Size: 4442
AleI CACnn'nnGTG
Cuts at: 710 710
Size: 4442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1255 1255
Size: 4442
AlwNI CAG_nnn'CTG
Cuts at: 1747 3314 1747
Size: 1567 2875
ApaI G_GGCC'C
Cuts at: 494 494
Size: 4442
ApaLI G'TGCA_C
Cuts at: 3212 3212
Size: 4442
ApoI r'AATT_y
Cuts at: 1446 3795 4051 1446
Size: 2349 256 1837
Fragments arranged by size:
2349 1837 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4442
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
AvaI C'yCGr_G
Cuts at: 192 486 787 1462 192
Size: 294 301 675 3172
Fragments arranged by size:
3172 675 301 294
BamHI G'GATC_C
Cuts at: 382 1467 382
Size: 1085 3357
BanI G'GyrC_C
Cuts at: 329 669 1458 1872 2120 2155 329
Size: 340 789 414 248 35 2616
Fragments arranged by size:
2616 789 414 340 248 35
BanII G_rGCy'C
Cuts at: 494 974 1136 1456 2486 494
Size: 480 162 320 1030 2450
Fragments arranged by size:
2450 1030 480 320 162
BbeI G_GCGC'C
Cuts at: 1876 2124 1876
Size: 248 4194
BbvCI CC'TCA_GC
Cuts at: 199 199
Size: 4442
BciVI GTATCCnnnnn_n'
Cuts at: 2340 3101 4442 2340
Size: 761 1341 2340
Fragments arranged by size:
2340 1341 761
BclI T'GATC_A
Cuts at: 1962 1962
Size: 4442
BglI GCCn_nnn'nGGC
Cuts at: 631 827 631
Size: 196 4246
BglII A'GATC_T
Cuts at: 1957 3994 1957
Size: 2037 2405
BlpI GC'TnA_GC
Cuts at: 1554 1554
Size: 4442
Bme1580I G_kGCm'C
Cuts at: 494 674 788 2067 2160 3216 494
Size: 180 114 1279 93 1056 1720
Fragments arranged by size:
1720 1279 1056 180 114 93
BmgBI CAC'GTC
Cuts at: 285 285
Size: 4442
BmrI ACTGGGnnnn_n'
Cuts at: 1654 1735 2070 4214 1654
Size: 81 335 2144 1882
Fragments arranged by size:
2144 1882 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 350 813 2809 350
Size: 463 1996 1983
Fragments arranged by size:
1996 1983 463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1110 1273 1281 1326 1398 715
Size: 395 163 8 45 72 3759
Fragments arranged by size:
3759 395 163 72 45 8
Bpu10I CC'TnA_GC
Cuts at: 199 1315 199
Size: 1116 3326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1618 2497 2989 3287 643
Size: 975 879 492 298 1798
Fragments arranged by size:
1798 975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4146 4146
Size: 4442
BsaAI yAC'GTr
Cuts at: 783 1795 783
Size: 1012 3430
BsaBI GATnn'nnATC
Cuts at: 6 1956 3993 6
Size: 1950 2037 455
Fragments arranged by size:
2037 1950 455
BsaHI Gr'CG_yC
Cuts at: 466 587 1051 1873 2121 2823 4319 466
Size: 121 464 822 248 702 1496 589
Fragments arranged by size:
1496 822 702 589 464 248 121
BsaWI w'CCGG_w
Cuts at: 799 1631 2152 2830 3104 3251 3662 799
Size: 832 521 678 274 147 411 1579
Fragments arranged by size:
1579 832 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 466 496 1294 1324 1396 1426 418
Size: 30 18 30 798 30 72 30 3434
Fragments arranged by size:
3434 798 72 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 513 732 1107 256
Size: 257 219 375 3591
Fragments arranged by size:
3591 375 257 219
BseYI C'CCAG_C
Cuts at: 134 814 825 1867 2625 3202 3698 134
Size: 680 11 1042 758 577 496 878
Fragments arranged by size:
1042 878 758 680 577 496 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 4442
BsiEI CG_ry'CG
Cuts at: 104 626 1289 1984 2030 3238 104
Size: 522 663 695 46 1208 1308
Fragments arranged by size:
1308 1208 695 663 522 46
BsiHKAI G_wGCw'C
Cuts at: 164 1456 2234 2424 3216 164
Size: 1292 778 190 792 1390
Fragments arranged by size:
1390 1292 792 778 190
BsmI GAATG_Cn'
Cuts at: 1104 1104
Size: 4442
BsmBI CGTCTCn'nnnn_
Cuts at: 594 594
Size: 4442
Bsp1286I G_dGCh'C
Cuts at: 164 494 674 788 974 1136 1456 2067 2160
Size: 330 180 114 186 162 320 611 93
Cuts at: 2160 2234 2424 2486 3216 164
Size: 74 190 62 730 1390
Fragments arranged by size:
1390 730 611 330 320 190 186 180
162 114 93 74 62
BspEI T'CCGG_A
Cuts at: 799 3662 799
Size: 2863 1579
BspHI T'CATG_A
Cuts at: 3618 3618
Size: 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1492 1706 2008 2389 1492
Size: 214 302 381 3545
Fragments arranged by size:
3545 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2734 2788 4272 4437 50
Size: 2684 54 1484 165 55
Fragments arranged by size:
2684 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1128 2354 1128
Size: 1226 3216
BsrFI r'CCGG_y
Cuts at: 625 841 998 2440 2621 2830 625
Size: 216 157 1442 181 209 2237
Fragments arranged by size:
2237 1442 216 209 181 157
BssSI C'ACGA_G
Cuts at: 2713 3071 2713
Size: 358 4084
BstAPI GCAn_nnn'nTGC
Cuts at: 965 1819 965
Size: 854 3588
BstBI TT'CG_AA
Cuts at: 2803 2803
Size: 4442
BstEII G'GTnAC_C
Cuts at: 1422 1422
Size: 4442
BstXI CCAn_nnnn'nTGG
Cuts at: 712 712
Size: 4442
BstYI r'GATC_y
Cuts at: 1 382 1467 1934 1957 2292 2538 3550 3636
Size: 381 1085 467 23 335 246 1012 86
Cuts at: 3636 3648 3994 1
Size: 12 346 449
Fragments arranged by size:
1085 1012 467 449 381 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 317 345 477 711 1430 107
Size: 210 28 132 234 719 3119
Fragments arranged by size:
3119 719 234 210 132 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 587 2392 2573 2736 587
Size: 1805 181 163 2293
Fragments arranged by size:
2293 1805 181 163
BtsI GCAGTG_nn'
Cuts at: 1685 1685
Size: 4442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1110 1145 1110
Size: 35 4407
DraI TTT'AAA
Cuts at: 1441 1441
Size: 4442
DrdI GACnn_nn'nnGTC
Cuts at: 2148 3006 2148
Size: 858 3584
EaeI y'GGCC_r
Cuts at: 623 1286 2027 2201 2592 2619 3964 623
Size: 663 741 174 391 27 1345 1101
Fragments arranged by size:
1345 1101 741 663 391 174 27
EagI C'GGCC_G
Cuts at: 623 1286 2027 623
Size: 663 741 3038
Fragments arranged by size:
3038 741 663
EarI CTCTTCn'nnn_
Cuts at: 562 811 2465 2675 562
Size: 249 1654 210 2329
Fragments arranged by size:
2329 1654 249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 2650 2960 3106 4252 136
Size: 2514 310 146 1146 326
Fragments arranged by size:
2514 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 1454 1454
Size: 4442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1110 1273 1281 1317 1326 1398 2267 2699
Size: 395 163 8 36 9 72 869 432
Cuts at: 2699 3446 3714 715
Size: 747 268 1443
Fragments arranged by size:
1443 869 747 432 395 268 163 72
36 9 8
EcoO109I rG'GnC_Cy
Cuts at: 490 491 1110 1581 490
Size: 1 619 471 3351
Fragments arranged by size:
3351 619 471 1
EcoRI G'AATT_C
Cuts at: 1446 1446
Size: 4442
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4442
FspI TGC'GCA
Cuts at: 2223 2223
Size: 4442
HaeII r_GCGC'y
Cuts at: 1876 2124 3146 4125 1876
Size: 248 1022 979 2193
Fragments arranged by size:
2193 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2284 2314 2316 2346 2284
Size: 30 2 30 4380
Fragments arranged by size:
4380 30 30 2
HincII GTy'rAC
Cuts at: 749 1481 749
Size: 732 3710
KasI G'GCGC_C
Cuts at: 1872 2120 1872
Size: 248 4194
KpnI G_GTAC'C
Cuts at: 333 1462 333
Size: 1129 3313
MluI A'CGCG_T
Cuts at: 3875 3875
Size: 4442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 361 3113 3297 361
Size: 2752 184 1506
Fragments arranged by size:
2752 1506 184
MscI TGG'CCA
Cuts at: 2203 2203
Size: 4442
MslI CAynn'nnrTG
Cuts at: 112 710 1369 1429 2431 2558 112
Size: 598 659 60 1002 127 1996
Fragments arranged by size:
1996 1002 659 598 127 60
MspA1I CmG'CkG
Cuts at: 234 1553 1867 2227 3240 3485 234
Size: 1319 314 360 1013 245 1191
Fragments arranged by size:
1319 1191 1013 360 314 245
NaeI GCC'GGC
Cuts at: 627 2623 627
Size: 1996 2446
NarI GG'CG_CC
Cuts at: 1873 2121 1873
Size: 248 4194
NcoI C'CATG_G
Cuts at: 107 1430 107
Size: 1323 3119
NgoMIV G'CCGG_C
Cuts at: 625 2621 625
Size: 1996 2446
NspI r_CATG'y
Cuts at: 1308 1353 1495 1995 2902 1308
Size: 45 142 500 907 2848
Fragments arranged by size:
2848 907 500 142 45
PasI CC'CwG_GG
Cuts at: 414 729 414
Size: 315 4127
PciI A'CATG_T
Cuts at: 1304 2898 1304
Size: 1594 2848
PflMI CCAn_nnn'nTGG
Cuts at: 1088 1088
Size: 4442
PmeI GTTT'AAAC
Cuts at: 1441 1441
Size: 4442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4410
PpuMI rG'GwC_Cy
Cuts at: 1110 1110
Size: 4442
PshAI GACnn'nnGTC
Cuts at: 1305 1305
Size: 4442
PspOMI G'GGCC_C
Cuts at: 490 490
Size: 4442
PstI C_TGCA'G
Cuts at: 1035 1489 2174 1035
Size: 454 685 3303
Fragments arranged by size:
3303 685 454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4442
PvuII CAG'CTG
Cuts at: 1867 2227 1867
Size: 360 4082
RsrII CG'GwC_CG
Cuts at: 2637 2637
Size: 4442
SacI G_AGCT'C
Cuts at: 1456 1456
Size: 4442
SalI G'TCGA_C
Cuts at: 747 1479 747
Size: 732 3710
SanDI GG'GwC_CC
Cuts at: 1110 1110
Size: 4442
SapI GCTCTTCn'nnn_
Cuts at: 2465 2675 2465
Size: 210 4232
SbfI CC_TGCA'GG
Cuts at: 1489 1489
Size: 4442
ScaI AGT'ACT
Cuts at: 3959 3959
Size: 4442
SexAI A'CCwGG_T
Cuts at: 665 665
Size: 4442
SfcI C'TryA_G
Cuts at: 33 1031 1485 2170 3163 3354 33
Size: 998 454 685 993 191 1121
Fragments arranged by size:
1121 998 993 685 454 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 631 631
Size: 4442
SfoI GGC'GCC
Cuts at: 1874 2122 1874
Size: 248 4194
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4442
SmaI CCC'GGG
Cuts at: 194 488 1464 194
Size: 294 976 3172
Fragments arranged by size:
3172 976 294
SmlI C'TyrA_G
Cuts at: 658 1597 2512 3004 3266 658
Size: 939 915 492 262 1834
Fragments arranged by size:
1834 939 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4442
SphI G_CATG'C
Cuts at: 1495 1995 1495
Size: 500 3942
SspI AAT'ATT
Cuts at: 1155 1155
Size: 4442
StyI C'CwwG_G
Cuts at: 107 866 1430 1576 1930 107
Size: 759 564 146 354 2619
Fragments arranged by size:
2619 759 564 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 297 721 1423 2025 2158 2617 2826 126
Size: 171 424 702 602 133 459 209 1742
Fragments arranged by size:
1742 702 602 459 424 209 171 133
TatI w'GTAC_w
Cuts at: 791 3957 791
Size: 3166 1276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 466 498 1041 1073 1294 1326 1366
Size: 32 16 32 543 32 221 32 40
Cuts at: 1366 1396 1398 1428 3959 3991 418
Size: 30 2 30 2531 32 869
Fragments arranged by size:
2531 869 543 221 40 32 32 32
32 32 30 30 16 2
Tth111I GACn'n_nGTC
Cuts at: 1114 2239 1114
Size: 1125 3317
XbaI T'CTAG_A
Cuts at: 1473 1473
Size: 4442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 822 1937 822
Size: 1115 3327
XmaI C'CCGG_G
Cuts at: 192 486 1462 192
Size: 294 976 3172
Fragments arranged by size:
3172 976 294
ZraI GAC'GTC
Cuts at: 467 588 1052 467
Size: 121 464 3857
Fragments arranged by size:
3857 464 121
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PsiI PspXI PsrI SacII SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI