Restriction map for
pF1KB8367
(Circular) (Six-base) MAPSORT of: pF1KB8367.seq Check: 666 from: 1 to: 4007
pF1KB8367 4007 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 767 767
Size: 4007
AccI GT'mk_AC
Cuts at: 326 1045 326
Size: 719 3288
Acc65I G'GTAC_C
Cuts at: 1023 1023
Size: 4007
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2264 3011 3279 1832
Size: 432 747 268 2560
Fragments arranged by size:
2560 747 432 268
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 646 646
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 598 1312 2879 598
Size: 714 1567 1726
Fragments arranged by size:
1726 1567 714
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 1011 3360 3616 1011
Size: 2349 256 1402
Fragments arranged by size:
2349 1402 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 1023 1437 1685 1720 1023
Size: 414 248 35 3310
Fragments arranged by size:
3310 414 248 35
BanII G_rGCy'C
Cuts at: 1021 2051 1021
Size: 1030 2977
BbeI G_GCGC'C
Cuts at: 1441 1689 1441
Size: 248 3759
BbsI GAAGACnn'nnnn_
Cuts at: 277 277
Size: 4007
BciVI GTATCCnnnnn_n'
Cuts at: 471 1905 2666 4007 471
Size: 1434 761 1341 471
Fragments arranged by size:
1434 1341 761 471
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 447 1632 1725 2781 447
Size: 1185 93 1056 1673
Fragments arranged by size:
1673 1185 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 313 1219 1300 1635 3779 313
Size: 906 81 335 2144 541
Fragments arranged by size:
2144 906 541 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 491 755 267
Size: 224 264 3519
Fragments arranged by size:
3519 264 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 1360 1360
Size: 4007
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 1438 1686 2388 3884 1438
Size: 248 702 1496 1561
Fragments arranged by size:
1561 1496 702 248
BsaWI w'CCGG_w
Cuts at: 389 1196 1717 2395 2669 2816 3227 389
Size: 807 521 678 274 147 411 1169
Fragments arranged by size:
1169 807 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 124 129 132 124
Size: 5 3 3999
Fragments arranged by size:
3999 5 3
BseYI C'CCAG_C
Cuts at: 1432 2190 2767 3263 1432
Size: 758 577 496 2176
Fragments arranged by size:
2176 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1549 1595 2803 104
Size: 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1021 1799 1989 2781 1021
Size: 778 190 792 2247
Fragments arranged by size:
2247 792 778 190
BsmI GAATG_Cn'
Cuts at: 800 800
Size: 4007
Bsp1286I G_dGCh'C
Cuts at: 447 1021 1632 1725 1799 1989 2051 2781 447
Size: 574 611 93 74 190 62 730 1673
Fragments arranged by size:
1673 730 611 574 190 93 74 62
BspEI T'CCGG_A
Cuts at: 389 3227 389
Size: 2838 1169
BspHI T'CATG_A
Cuts at: 3183 3183
Size: 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 767 1057 1271 1573 1954 767
Size: 290 214 302 381 2820
Fragments arranged by size:
2820 381 302 290 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstYI r'GATC_y
Cuts at: 1 1032 1499 1522 1857 2103 3115 3201 3213
Size: 1031 467 23 335 246 1012 86 12
Cuts at: 3213 3559 1
Size: 346 449
Fragments arranged by size:
1031 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 1250 1250
Size: 4007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 801 836 801
Size: 35 3972
DraI TTT'AAA
Cuts at: 357 1006 357
Size: 649 3358
DrdI GACnn_nn'nnGTC
Cuts at: 1713 2571 1713
Size: 858 3149
EaeI y'GGCC_r
Cuts at: 1592 1766 2157 2184 3529 1592
Size: 174 391 27 1345 2070
Fragments arranged by size:
2070 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1592 1592
Size: 4007
EarI CTCTTCn'nnn_
Cuts at: 347 467 540 925 2030 2240 347
Size: 120 73 385 1105 210 2114
Fragments arranged by size:
2114 1105 385 210 120 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1019
Size: 4007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 491 755 1832 2264 3011 3279 267
Size: 224 264 1077 432 747 268 995
Fragments arranged by size:
1077 995 747 432 268 264 224
EcoNI CCTnn'n_nnAGG
Cuts at: 472 472
Size: 4007
EcoO109I rG'GnC_Cy
Cuts at: 1146 1146
Size: 4007
EcoRI G'AATT_C
Cuts at: 1011 1011
Size: 4007
EcoRV GAT'ATC
Cuts at: 772 772
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 4007
FspI TGC'GCA
Cuts at: 1788 1788
Size: 4007
HaeII r_GCGC'y
Cuts at: 1441 1689 2711 3690 1441
Size: 248 1022 979 1758
Fragments arranged by size:
1758 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1849 1879 1881 1911 1849
Size: 30 2 30 3945
Fragments arranged by size:
3945 30 30 2
HincII GTy'rAC
Cuts at: 1046 1046
Size: 4007
KasI G'GCGC_C
Cuts at: 1437 1685 1437
Size: 248 3759
KpnI G_GTAC'C
Cuts at: 1027 1027
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 289 747 2678 2862 289
Size: 458 1931 184 1434
Fragments arranged by size:
1931 1434 458 184
MscI TGG'CCA
Cuts at: 1768 1768
Size: 4007
MslI CAynn'nnrTG
Cuts at: 958 1996 2123 958
Size: 1038 127 2842
Fragments arranged by size:
2842 1038 127
MspA1I CmG'CkG
Cuts at: 1118 1432 1792 2805 3050 1118
Size: 314 360 1013 245 2075
Fragments arranged by size:
2075 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 1438 1686 1438
Size: 248 3759
NcoI C'CATG_G
Cuts at: 107 107
Size: 4007
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PasI CC'CwG_GG
Cuts at: 168 168
Size: 4007
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PflMI CCAn_nnn'nTGG
Cuts at: 468 468
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PsiI TTA'TAA
Cuts at: 997 997
Size: 4007
PstI C_TGCA'G
Cuts at: 1054 1739 1054
Size: 685 3322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 1432 1792 1432
Size: 360 3647
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 1021 1021
Size: 4007
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 2030 2240 2030
Size: 210 3797
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SexAI A'CCwGG_T
Cuts at: 155 155
Size: 4007
SfcI C'TryA_G
Cuts at: 33 1050 1735 2728 2919 33
Size: 1017 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 191
SfoI GGC'GCC
Cuts at: 1439 1687 1439
Size: 248 3759
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 1162 2077 2569 2831 1162
Size: 915 492 262 2338
Fragments arranged by size:
2338 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
SspI AAT'ATT
Cuts at: 742 742
Size: 4007
StuI AGG'CCT
Cuts at: 184 184
Size: 4007
StyI C'CwwG_G
Cuts at: 107 873 1141 1495 107
Size: 766 268 354 2619
Fragments arranged by size:
2619 766 354 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1590 1723 2182 2391 1590
Size: 133 459 209 3206
Fragments arranged by size:
3206 459 209 133
TatI w'GTAC_w
Cuts at: 3522 3522
Size: 4007
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 973 1005 3524 3556 973
Size: 32 2519 32 1424
Fragments arranged by size:
2519 1424 32 32
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 1038 1038
Size: 4007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1502 1502
Size: 4007
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI