Restriction map for
pF1KB8369
(Circular) (Six-base) MAPSORT of: pF1KB8369.seq Check: 1583 from: 1 to: 3707
pF1KB8369 3707 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 696 696
Size: 3707
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1532 1964 2711 2979 1532
Size: 432 747 268 2260
Fragments arranged by size:
2260 747 432 268
AflIII A'CryG_T
Cuts at: 1059 2163 3140 1059
Size: 1104 977 1626
Fragments arranged by size:
1626 1104 977
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 288 711 3060 3316 288
Size: 423 2349 256 679
Fragments arranged by size:
2349 679 423 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3707
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 233 361 727 233
Size: 128 366 3213
Fragments arranged by size:
3213 366 128
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 397 408 453 723 1137 1385 1420 397
Size: 11 45 270 414 248 35 2684
Fragments arranged by size:
2684 414 270 248 45 35 11
BanII G_rGCy'C
Cuts at: 139 471 721 1751 139
Size: 332 250 1030 2095
Fragments arranged by size:
2095 1030 332 250
BbeI G_GCGC'C
Cuts at: 457 1141 1389 457
Size: 684 248 2775
Fragments arranged by size:
2775 684 248
BbsI GAAGACnn'nnnn_
Cuts at: 696 696
Size: 3707
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglI GCCn_nnn'nGGC
Cuts at: 166 431 166
Size: 265 3442
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 1332 1425 2481 1332
Size: 93 1056 2558
Fragments arranged by size:
2558 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 919 1000 1335 3479 919
Size: 81 335 2144 1147
Fragments arranged by size:
2144 1147 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 166
Size: 3707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 1762 2254 2552 883
Size: 879 492 298 2038
Fragments arranged by size:
2038 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 158 454 667 693 1138 1386 2088 3584 158
Size: 296 213 26 445 248 702 1496 281
Fragments arranged by size:
1496 702 445 296 281 248 213 26
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1132 1890 2467 2963 1132
Size: 758 577 496 1876
Fragments arranged by size:
1876 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 176 721 1499 1689 2481 176
Size: 545 778 190 792 1402
Fragments arranged by size:
1402 792 778 545 190
Bsp1286I G_dGCh'C
Cuts at: 139 176 471 721 1332 1425 1499 1689 1751
Size: 37 295 250 611 93 74 190 62
Cuts at: 1751 2481 139
Size: 730 1365
Fragments arranged by size:
1365 730 611 295 250 190 93 74
62 37
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 579 757 971 1273 1654 579
Size: 178 214 302 381 2632
Fragments arranged by size:
2632 381 302 214 178
BsrBI CCG'CTC
Cuts at: 50 150 1999 2053 3537 3702 50
Size: 100 1849 54 1484 165 55
Fragments arranged by size:
1849 1484 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 553 1619 553
Size: 1066 2641
BsrFI r'CCGG_y
Cuts at: 411 649 1705 1886 2095 411
Size: 238 1056 181 209 2023
Fragments arranged by size:
2023 1056 238 209 181
BssSI C'ACGA_G
Cuts at: 1978 2336 1978
Size: 358 3349
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1084
Size: 3707
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstYI r'GATC_y
Cuts at: 1 732 1199 1222 1557 1803 2815 2901 2913
Size: 731 467 23 335 246 1012 86 12
Cuts at: 2913 3259 1
Size: 346 449
Fragments arranged by size:
1012 731 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 382 382
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 249 950 249
Size: 701 3006
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 516 1413 2271 516
Size: 897 858 1952
Fragments arranged by size:
1952 897 858
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 208 218 260 1730 1940 208
Size: 10 42 1470 210 1975
Fragments arranged by size:
1975 1470 210 42 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 184 1915 2225 2371 3517 184
Size: 1731 310 146 1146 374
Fragments arranged by size:
1731 1146 374 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1532 1964 2711 2979 166
Size: 1366 432 747 268 894
Fragments arranged by size:
1366 894 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 846 846
Size: 3707
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 372 1726 372
Size: 1354 2353
FspI TGC'GCA
Cuts at: 552 1488 552
Size: 936 2771
HaeII r_GCGC'y
Cuts at: 457 1141 1389 2411 3390 457
Size: 684 248 1022 979 774
Fragments arranged by size:
1022 979 774 684 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1579 1581 1611 1549
Size: 30 2 30 3645
Fragments arranged by size:
3645 30 30 2
HincII GTy'rAC
Cuts at: 230 746 230
Size: 516 3191
HpaI GTT'AAC
Cuts at: 230 230
Size: 3707
KasI G'GCGC_C
Cuts at: 453 1137 1385 453
Size: 684 248 2775
Fragments arranged by size:
2775 684 248
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 500 2378 2562 500
Size: 1878 184 1645
Fragments arranged by size:
1878 1645 184
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 1696 1823 1696
Size: 127 3580
MspA1I CmG'CkG
Cuts at: 384 437 818 1132 1492 2505 2750 384
Size: 53 381 314 360 1013 245 1341
Fragments arranged by size:
1341 1013 381 360 314 245 53
NaeI GCC'GGC
Cuts at: 413 1888 413
Size: 1475 2232
NarI GG'CG_CC
Cuts at: 454 1138 1386 454
Size: 684 248 2775
Fragments arranged by size:
2775 684 248
NgoMIV G'CCGG_C
Cuts at: 411 1886 411
Size: 1475 2232
NspI r_CATG'y
Cuts at: 760 1260 2167 760
Size: 500 907 2300
Fragments arranged by size:
2300 907 500
PciI A'CATG_T
Cuts at: 2163 2163
Size: 3707
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PstI C_TGCA'G
Cuts at: 196 256 754 1439 196
Size: 60 498 685 2464
Fragments arranged by size:
2464 685 498 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SacII CC_GC'GG
Cuts at: 385 385
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 1730 1940 1730
Size: 210 3497
SbfI CC_TGCA'GG
Cuts at: 196 754 196
Size: 558 3149
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 192 252 372 750 1435 2428 2619 33
Size: 159 60 120 378 685 993 191 1121
Fragments arranged by size:
1121 993 685 378 191 159 120 60
SfoI GGC'GCC
Cuts at: 455 1139 1387 455
Size: 684 248 2775
Fragments arranged by size:
2775 684 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 235 729 235
Size: 494 3213
SmlI C'TyrA_G
Cuts at: 862 1777 2269 2531 862
Size: 915 492 262 2038
Fragments arranged by size:
2038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 841 1195 841
Size: 354 3353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 3222 3222
Size: 3707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 233 727 233
Size: 494 3213
XmnI GAAnn'nnTTC
Cuts at: 202 202
Size: 3707
ZraI GAC'GTC
Cuts at: 694 694
Size: 3707
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI