Restriction map for
pF1KB8372
(Circular) (Six-base) MAPSORT of: pF1KB8372.seq Check: 8530 from: 1 to: 3608
pF1KB8372 3608 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 254 646 254
Size: 392 3216
Acc65I G'GTAC_C
Cuts at: 624 624
Size: 3608
AclI AA'CG_TT
Cuts at: 3448 3448
Size: 3608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 1865 2612 2880 1433
Size: 432 747 268 2161
Fragments arranged by size:
2161 747 432 268
AflII C'TTAA_G
Cuts at: 525 525
Size: 3608
AflIII A'CryG_T
Cuts at: 960 2064 3041 960
Size: 1104 977 1527
Fragments arranged by size:
1527 1104 977
AgeI A'CCGG_T
Cuts at: 1996 1996
Size: 3608
AlwNI CAG_nnn'CTG
Cuts at: 913 2480 913
Size: 1567 2041
ApaLI G'TGCA_C
Cuts at: 2378 2378
Size: 3608
ApoI r'AATT_y
Cuts at: 121 612 2961 3217 121
Size: 491 2349 256 512
Fragments arranged by size:
2349 512 491 256
AseI AT'TA_AT
Cuts at: 20 587 20
Size: 567 3041
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
AvaI C'yCGr_G
Cuts at: 390 628 390
Size: 238 3370
BamHI G'GATC_C
Cuts at: 516 633 516
Size: 117 3491
BanI G'GyrC_C
Cuts at: 451 624 1038 1286 1321 451
Size: 173 414 248 35 2738
Fragments arranged by size:
2738 414 248 173 35
BanII G_rGCy'C
Cuts at: 410 622 1652 410
Size: 212 1030 2366
Fragments arranged by size:
2366 1030 212
BbeI G_GCGC'C
Cuts at: 455 1042 1290 455
Size: 587 248 2773
Fragments arranged by size:
2773 587 248
BbsI GAAGACnn'nnnn_
Cuts at: 148 148
Size: 3608
BciVI GTATCCnnnnn_n'
Cuts at: 1506 2267 3608 1506
Size: 761 1341 1506
Fragments arranged by size:
1506 1341 761
BclI T'GATC_A
Cuts at: 1128 1128
Size: 3608
BglII A'GATC_T
Cuts at: 1123 3160 1123
Size: 2037 1571
BlpI GC'TnA_GC
Cuts at: 187 720 187
Size: 533 3075
Bme1580I G_kGCm'C
Cuts at: 1233 1326 2382 1233
Size: 93 1056 2459
Fragments arranged by size:
2459 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 820 901 1236 3380 820
Size: 81 335 2144 1048
Fragments arranged by size:
2144 1048 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 141 1975 141
Size: 1834 1774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 196 152
Size: 44 3564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1663 2155 2453 784
Size: 879 492 298 1939
Fragments arranged by size:
1939 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 415 3312 415
Size: 2897 711
BsaAI yAC'GTr
Cuts at: 961 961
Size: 3608
BsaBI GATnn'nnATC
Cuts at: 6 1122 3159 6
Size: 1116 2037 455
Fragments arranged by size:
2037 1116 455
BsaHI Gr'CG_yC
Cuts at: 452 1039 1287 1989 3485 452
Size: 587 248 702 1496 575
Fragments arranged by size:
1496 702 587 575 248
BsaWI w'CCGG_w
Cuts at: 797 1318 1996 2270 2417 2828 797
Size: 521 678 274 147 411 1577
Fragments arranged by size:
1577 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1033 1791 2368 2864 1033
Size: 758 577 496 1777
Fragments arranged by size:
1777 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 457
Size: 3608
BsiEI CG_ry'CG
Cuts at: 104 1150 1196 2404 104
Size: 1046 46 1208 1308
Fragments arranged by size:
1308 1208 1046 46
BsiHKAI G_wGCw'C
Cuts at: 188 410 622 1400 1590 2382 188
Size: 222 212 778 190 792 1414
Fragments arranged by size:
1414 792 778 222 212 190
Bsp1286I G_dGCh'C
Cuts at: 188 410 622 1233 1326 1400 1590 1652 2382
Size: 222 212 611 93 74 190 62 730
Cuts at: 2382 188
Size: 1414
Fragments arranged by size:
1414 730 611 222 212 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2828 2828
Size: 3608
BspHI T'CATG_A
Cuts at: 2784 2784
Size: 3608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 479 658 872 1174 1555 479
Size: 179 214 302 381 2532
Fragments arranged by size:
2532 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 1900 1954 3438 3603 50
Size: 1850 54 1484 165 55
Fragments arranged by size:
1850 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1520 1520
Size: 3608
BsrFI r'CCGG_y
Cuts at: 395 1606 1787 1996 395
Size: 1211 181 209 2007
Fragments arranged by size:
2007 1211 209 181
BssSI C'ACGA_G
Cuts at: 1879 2237 1879
Size: 358 3250
BstAPI GCAn_nnn'nTGC
Cuts at: 480 985 480
Size: 505 3103
BstBI TT'CG_AA
Cuts at: 1969 1969
Size: 3608
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3608
BstYI r'GATC_y
Cuts at: 1 323 516 633 1100 1123 1458 1704 2716
Size: 322 193 117 467 23 335 246 1012
Cuts at: 2716 2802 2814 3160 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 322 246 193
117 86 23 12
BtgI C'CryG_G
Cuts at: 437 437
Size: 3608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1558 1739 1902 1558
Size: 181 163 3264
Fragments arranged by size:
3264 181 163
BtsI GCAGTG_nn'
Cuts at: 474 851 474
Size: 377 3231
DraI TTT'AAA
Cuts at: 607 607
Size: 3608
DraIII CAC_nnn'GTG
Cuts at: 480 480
Size: 3608
DrdI GACnn_nn'nnGTC
Cuts at: 1314 2172 1314
Size: 858 2750
EaeI y'GGCC_r
Cuts at: 236 1193 1367 1758 1785 3130 236
Size: 957 174 391 27 1345 714
Fragments arranged by size:
1345 957 714 391 174 27
EagI C'GGCC_G
Cuts at: 1193 1193
Size: 3608
EarI CTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 304 418 1816 2126 2272 3418 304
Size: 114 1398 310 146 1146 494
Fragments arranged by size:
1398 1146 494 310 146 114
EcoICRI GAG'CTC
Cuts at: 408 620 408
Size: 212 3396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 196 1433 1865 2612 2880 152
Size: 44 1237 432 747 268 880
Fragments arranged by size:
1237 880 747 432 268 44
EcoNI CCTnn'n_nnAGG
Cuts at: 340 340
Size: 3608
EcoO109I rG'GnC_Cy
Cuts at: 747 747
Size: 3608
EcoRI G'AATT_C
Cuts at: 121 612 121
Size: 491 3117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1627 1627
Size: 3608
FspI TGC'GCA
Cuts at: 1389 1389
Size: 3608
HaeII r_GCGC'y
Cuts at: 455 1042 1290 2312 3291 455
Size: 587 248 1022 979 772
Fragments arranged by size:
1022 979 772 587 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1480 1482 1512 1450
Size: 30 2 30 3546
Fragments arranged by size:
3546 30 30 2
HincII GTy'rAC
Cuts at: 255 532 647 255
Size: 277 115 3216
Fragments arranged by size:
3216 277 115
KasI G'GCGC_C
Cuts at: 451 1038 1286 451
Size: 587 248 2773
Fragments arranged by size:
2773 587 248
KpnI G_GTAC'C
Cuts at: 628 628
Size: 3608
MluI A'CGCG_T
Cuts at: 3041 3041
Size: 3608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2463 2279
Size: 184 3424
MscI TGG'CCA
Cuts at: 1369 1369
Size: 3608
MslI CAynn'nnrTG
Cuts at: 507 1597 1724 507
Size: 1090 127 2391
Fragments arranged by size:
2391 1090 127
MspA1I CmG'CkG
Cuts at: 439 719 1033 1393 2406 2651 439
Size: 280 314 360 1013 245 1396
Fragments arranged by size:
1396 1013 360 314 280 245
NaeI GCC'GGC
Cuts at: 397 1789 397
Size: 1392 2216
NarI GG'CG_CC
Cuts at: 452 1039 1287 452
Size: 587 248 2773
Fragments arranged by size:
2773 587 248
NgoMIV G'CCGG_C
Cuts at: 395 1787 395
Size: 1392 2216
NspI r_CATG'y
Cuts at: 661 1161 2068 661
Size: 500 907 2201
Fragments arranged by size:
2201 907 500
PciI A'CATG_T
Cuts at: 2064 2064
Size: 3608
PmeI GTTT'AAAC
Cuts at: 607 607
Size: 3608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2779 2747
Size: 32 3576
PstI C_TGCA'G
Cuts at: 323 655 1340 323
Size: 332 685 2591
Fragments arranged by size:
2591 685 332
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3608
PvuII CAG'CTG
Cuts at: 1033 1393 1033
Size: 360 3248
RsrII CG'GwC_CG
Cuts at: 1803 1803
Size: 3608
SacI G_AGCT'C
Cuts at: 410 622 410
Size: 212 3396
SacII CC_GC'GG
Cuts at: 440 440
Size: 3608
SalI G'TCGA_C
Cuts at: 253 645 253
Size: 392 3216
SapI GCTCTTCn'nnn_
Cuts at: 1631 1841 1631
Size: 210 3398
SbfI CC_TGCA'GG
Cuts at: 655 655
Size: 3608
ScaI AGT'ACT
Cuts at: 3125 3125
Size: 3608
SfcI C'TryA_G
Cuts at: 33 319 651 1336 2329 2520 33
Size: 286 332 685 993 191 1121
Fragments arranged by size:
1121 993 685 332 286 191
SfoI GGC'GCC
Cuts at: 453 1040 1288 453
Size: 587 248 2773
Fragments arranged by size:
2773 587 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3608
SmaI CCC'GGG
Cuts at: 392 630 392
Size: 238 3370
SmlI C'TyrA_G
Cuts at: 525 763 1678 2170 2432 525
Size: 238 915 492 262 1701
Fragments arranged by size:
1701 915 492 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3608
SphI G_CATG'C
Cuts at: 661 1161 661
Size: 500 3108
StyI C'CwwG_G
Cuts at: 742 1096 742
Size: 354 3254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 160 439 1191 1324 1783 1992 160
Size: 279 752 133 459 209 1776
Fragments arranged by size:
1776 752 459 279 209 133
TatI w'GTAC_w
Cuts at: 3123 3123
Size: 3608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 3576
Tth111I GACn'n_nGTC
Cuts at: 1405 1405
Size: 3608
XbaI T'CTAG_A
Cuts at: 639 639
Size: 3608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1103 1103
Size: 3608
XmaI C'CCGG_G
Cuts at: 390 628 390
Size: 238 3370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI