Restriction map for
pF1KB8373
(Circular) (Six-base) MAPSORT of: pF1KB8373.seq Check: 2485 from: 1 to: 3626
pF1KB8373 3626 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 664 664
Size: 3626
Acc65I G'GTAC_C
Cuts at: 642 642
Size: 3626
AclI AA'CG_TT
Cuts at: 3466 3466
Size: 3626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1451 1883 2630 2898 457
Size: 994 432 747 268 1185
Fragments arranged by size:
1185 994 747 432 268
AflIII A'CryG_T
Cuts at: 978 2082 3059 978
Size: 1104 977 1545
Fragments arranged by size:
1545 1104 977
AgeI A'CCGG_T
Cuts at: 2014 2014
Size: 3626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 496 496
Size: 3626
AlwNI CAG_nnn'CTG
Cuts at: 136 931 2498 136
Size: 795 1567 1264
Fragments arranged by size:
1567 1264 795
ApaLI G'TGCA_C
Cuts at: 2396 2396
Size: 3626
ApoI r'AATT_y
Cuts at: 115 431 630 2979 3235 115
Size: 316 199 2349 256 506
Fragments arranged by size:
2349 506 316 256 199
AseI AT'TA_AT
Cuts at: 20 20
Size: 3626
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
AvaI C'yCGr_G
Cuts at: 646 646
Size: 3626
BamHI G'GATC_C
Cuts at: 651 651
Size: 3626
BanI G'GyrC_C
Cuts at: 642 1056 1304 1339 642
Size: 414 248 35 2929
Fragments arranged by size:
2929 414 248 35
BanII G_rGCy'C
Cuts at: 640 1670 640
Size: 1030 2596
BbeI G_GCGC'C
Cuts at: 1060 1308 1060
Size: 248 3378
BciVI GTATCCnnnnn_n'
Cuts at: 1524 2285 3626 1524
Size: 761 1341 1524
Fragments arranged by size:
1524 1341 761
BclI T'GATC_A
Cuts at: 167 1146 167
Size: 979 2647
BglII A'GATC_T
Cuts at: 225 1141 3178 225
Size: 916 2037 673
Fragments arranged by size:
2037 916 673
BlpI GC'TnA_GC
Cuts at: 738 738
Size: 3626
Bme1580I G_kGCm'C
Cuts at: 1251 1344 2400 1251
Size: 93 1056 2477
Fragments arranged by size:
2477 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 838 919 1254 3398 838
Size: 81 335 2144 1066
Fragments arranged by size:
2144 1066 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 802 1681 2173 2471 400
Size: 402 879 492 298 1555
Fragments arranged by size:
1555 879 492 402 298
BsaI GGTCTCn'nnnn_
Cuts at: 3330 3330
Size: 3626
BsaAI yAC'GTr
Cuts at: 979 979
Size: 3626
BsaBI GATnn'nnATC
Cuts at: 6 1140 3177 6
Size: 1134 2037 455
Fragments arranged by size:
2037 1134 455
BsaHI Gr'CG_yC
Cuts at: 1057 1305 2007 3503 1057
Size: 248 702 1496 1180
Fragments arranged by size:
1496 1180 702 248
BsaWI w'CCGG_w
Cuts at: 303 815 1336 2014 2288 2435 2846 303
Size: 512 521 678 274 147 411 1083
Fragments arranged by size:
1083 678 521 512 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 631 631
Size: 3626
BseYI C'CCAG_C
Cuts at: 129 1051 1809 2386 2882 129
Size: 922 758 577 496 873
Fragments arranged by size:
922 873 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1168 1214 2422 104
Size: 1064 46 1208 1308
Fragments arranged by size:
1308 1208 1064 46
BsiHKAI G_wGCw'C
Cuts at: 254 296 640 1418 1608 2400 254
Size: 42 344 778 190 792 1480
Fragments arranged by size:
1480 792 778 344 190 42
Bsp1286I G_dGCh'C
Cuts at: 254 296 640 1251 1344 1418 1608 1670 2400
Size: 42 344 611 93 74 190 62 730
Cuts at: 2400 254
Size: 1480
Fragments arranged by size:
1480 730 611 344 190 93 74 62
42
BspEI T'CCGG_A
Cuts at: 303 2846 303
Size: 2543 1083
BspHI T'CATG_A
Cuts at: 2802 2802
Size: 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 676 890 1192 1573 676
Size: 214 302 381 2729
Fragments arranged by size:
2729 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1918 1972 3456 3621 50
Size: 1868 54 1484 165 55
Fragments arranged by size:
1868 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 464 1538 464
Size: 1074 2552
BsrFI r'CCGG_y
Cuts at: 1624 1805 2014 1624
Size: 181 209 3236
Fragments arranged by size:
3236 209 181
BssSI C'ACGA_G
Cuts at: 1897 2255 1897
Size: 358 3268
BstAPI GCAn_nnn'nTGC
Cuts at: 1003 1003
Size: 3626
BstBI TT'CG_AA
Cuts at: 1987 1987
Size: 3626
BstYI r'GATC_y
Cuts at: 1 225 651 1118 1141 1476 1722 2734 2820
Size: 224 426 467 23 335 246 1012 86
Cuts at: 2820 2832 3178 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 426 346 335 246 224
86 23 12
BtgI C'CryG_G
Cuts at: 107 355 107
Size: 248 3378
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1576 1757 1920 1576
Size: 181 163 3282
Fragments arranged by size:
3282 181 163
BtsI GCAGTG_nn'
Cuts at: 134 156 264 869 134
Size: 22 108 605 2891
Fragments arranged by size:
2891 605 108 22
DraI TTT'AAA
Cuts at: 625 625
Size: 3626
DrdI GACnn_nn'nnGTC
Cuts at: 1332 2190 1332
Size: 858 2768
EaeI y'GGCC_r
Cuts at: 1211 1385 1776 1803 3148 1211
Size: 174 391 27 1345 1689
Fragments arranged by size:
1689 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1211 1211
Size: 3626
EarI CTCTTCn'nnn_
Cuts at: 528 554 1649 1859 528
Size: 26 1095 210 2295
Fragments arranged by size:
2295 1095 210 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 115 1834 2144 2290 3436 115
Size: 1719 310 146 1146 305
Fragments arranged by size:
1719 1146 310 305 146
EcoICRI GAG'CTC
Cuts at: 638 638
Size: 3626
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1451 1883 2630 2898 457
Size: 994 432 747 268 1185
Fragments arranged by size:
1185 994 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 765 765
Size: 3626
EcoRI G'AATT_C
Cuts at: 630 630
Size: 3626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 557 612 1645 557
Size: 55 1033 2538
Fragments arranged by size:
2538 1033 55
FspI TGC'GCA
Cuts at: 1407 1407
Size: 3626
HaeII r_GCGC'y
Cuts at: 1060 1308 2330 3309 1060
Size: 248 1022 979 1377
Fragments arranged by size:
1377 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1468 1498 1500 1530 1468
Size: 30 2 30 3564
Fragments arranged by size:
3564 30 30 2
HincII GTy'rAC
Cuts at: 665 665
Size: 3626
KasI G'GCGC_C
Cuts at: 1056 1304 1056
Size: 248 3378
KpnI G_GTAC'C
Cuts at: 646 646
Size: 3626
MluI A'CGCG_T
Cuts at: 3059 3059
Size: 3626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 547 2297 2481 547
Size: 1750 184 1692
Fragments arranged by size:
1750 1692 184
MscI TGG'CCA
Cuts at: 1387 1387
Size: 3626
MslI CAynn'nnrTG
Cuts at: 1615 1742 1615
Size: 127 3499
MspA1I CmG'CkG
Cuts at: 737 1051 1411 2424 2669 737
Size: 314 360 1013 245 1694
Fragments arranged by size:
1694 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1807 1807
Size: 3626
NarI GG'CG_CC
Cuts at: 1057 1305 1057
Size: 248 3378
NcoI C'CATG_G
Cuts at: 107 107
Size: 3626
NgoMIV G'CCGG_C
Cuts at: 1805 1805
Size: 3626
NspI r_CATG'y
Cuts at: 483 679 1179 2086 483
Size: 196 500 907 2023
Fragments arranged by size:
2023 907 500 196
PciI A'CATG_T
Cuts at: 2082 2082
Size: 3626
PflMI CCAn_nnn'nTGG
Cuts at: 270 270
Size: 3626
PmeI GTTT'AAAC
Cuts at: 625 625
Size: 3626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 3594
PstI C_TGCA'G
Cuts at: 151 475 673 1358 151
Size: 324 198 685 2419
Fragments arranged by size:
2419 685 324 198
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3626
PvuII CAG'CTG
Cuts at: 1051 1411 1051
Size: 360 3266
RsrII CG'GwC_CG
Cuts at: 1821 1821
Size: 3626
SacI G_AGCT'C
Cuts at: 640 640
Size: 3626
SalI G'TCGA_C
Cuts at: 663 663
Size: 3626
SapI GCTCTTCn'nnn_
Cuts at: 1649 1859 1649
Size: 210 3416
SbfI CC_TGCA'GG
Cuts at: 673 673
Size: 3626
ScaI AGT'ACT
Cuts at: 595 3143 595
Size: 2548 1078
SfcI C'TryA_G
Cuts at: 33 147 471 669 1354 2347 2538 33
Size: 114 324 198 685 993 191 1121
Fragments arranged by size:
1121 993 685 324 198 191 114
SfoI GGC'GCC
Cuts at: 1058 1306 1058
Size: 248 3378
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3626
SmaI CCC'GGG
Cuts at: 648 648
Size: 3626
SmlI C'TyrA_G
Cuts at: 379 781 1696 2188 2450 379
Size: 402 915 492 262 1555
Fragments arranged by size:
1555 915 492 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3626
SphI G_CATG'C
Cuts at: 483 679 1179 483
Size: 196 500 2930
Fragments arranged by size:
2930 500 196
StyI C'CwwG_G
Cuts at: 107 760 1114 107
Size: 653 354 2619
Fragments arranged by size:
2619 653 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1209 1342 1801 2010 1209
Size: 133 459 209 2825
Fragments arranged by size:
2825 459 209 133
TatI w'GTAC_w
Cuts at: 335 593 3141 335
Size: 258 2548 820
Fragments arranged by size:
2548 820 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3594
Tth111I GACn'n_nGTC
Cuts at: 1423 1423
Size: 3626
XbaI T'CTAG_A
Cuts at: 657 657
Size: 3626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1121 1121
Size: 3626
XmaI C'CCGG_G
Cuts at: 646 646
Size: 3626
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI