Restriction map for
pF1KB8376
(Circular) (Six-base) MAPSORT of: pF1KB8376.seq Check: 4965 from: 1 to: 4991
pF1KB8376 4991 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 690 690
Size: 4991
AatII G_ACGT'C
Cuts at: 815 815
Size: 4991
AccI GT'mk_AC
Cuts at: 2029 2029
Size: 4991
Acc65I G'GTAC_C
Cuts at: 2007 2007
Size: 4991
AclI AA'CG_TT
Cuts at: 4831 4831
Size: 4991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 3248 3995 4263 2816
Size: 432 747 268 3544
Fragments arranged by size:
3544 747 432 268
AflIII A'CryG_T
Cuts at: 2343 3447 4424 2343
Size: 1104 977 2910
Fragments arranged by size:
2910 1104 977
AgeI A'CCGG_T
Cuts at: 3379 3379
Size: 4991
AleI CACnn'nnGTG
Cuts at: 173 568 694 173
Size: 395 126 4470
Fragments arranged by size:
4470 395 126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 430 712 430
Size: 282 4709
AlwNI CAG_nnn'CTG
Cuts at: 1203 1398 2296 3863 1203
Size: 195 898 1567 2331
Fragments arranged by size:
2331 1567 898 195
ApaLI G'TGCA_C
Cuts at: 3761 3761
Size: 4991
ApoI r'AATT_y
Cuts at: 1995 4344 4600 1995
Size: 2349 256 2386
Fragments arranged by size:
2386 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 158 433 1258 1379 2011 158
Size: 275 825 121 632 3138
Fragments arranged by size:
3138 825 632 275 121
BamHI G'GATC_C
Cuts at: 2016 2016
Size: 4991
BanI G'GyrC_C
Cuts at: 175 540 588 688 1591 1660 1806 1832 2007
Size: 365 48 100 903 69 146 26 175
Cuts at: 2007 2421 2669 2704 175
Size: 414 248 35 2462
Fragments arranged by size:
2462 903 414 365 248 175 146 100
69 48 35 26
BanII G_rGCy'C
Cuts at: 656 871 1067 1626 1744 1877 2005 3035 656
Size: 215 196 559 118 133 128 1030 2612
Fragments arranged by size:
2612 1030 559 215 196 133 128 118
BbeI G_GCGC'C
Cuts at: 1595 1664 1836 2425 2673 1595
Size: 69 172 589 248 3913
Fragments arranged by size:
3913 589 248 172 69
BbsI GAAGACnn'nnnn_
Cuts at: 318 1010 318
Size: 692 4299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1491 1525 1491
Size: 34 4957
BciVI GTATCCnnnnn_n'
Cuts at: 2889 3650 4991 2889
Size: 761 1341 2889
Fragments arranged by size:
2889 1341 761
BclI T'GATC_A
Cuts at: 2511 2511
Size: 4991
BglI GCCn_nnn'nGGC
Cuts at: 1024 1024
Size: 4991
BglII A'GATC_T
Cuts at: 2506 4543 2506
Size: 2037 2954
BlpI GC'TnA_GC
Cuts at: 418 622 2103 418
Size: 204 1481 3306
Fragments arranged by size:
3306 1481 204
Bme1580I G_kGCm'C
Cuts at: 545 691 725 1809 2616 2709 3765 545
Size: 146 34 1084 807 93 1056 1771
Fragments arranged by size:
1771 1084 1056 807 146 93 34
BmrI ACTGGGnnnn_n'
Cuts at: 180 2203 2284 2619 4763 180
Size: 2023 81 335 2144 408
Fragments arranged by size:
2144 2023 408 335 81
BmtI G_CTAG'C
Cuts at: 1960 1960
Size: 4991
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 861 1152 1916 3358 861
Size: 291 764 1442 2494
Fragments arranged by size:
2494 1442 764 291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1003 1258 1378 1624 997
Size: 6 255 120 246 4364
Fragments arranged by size:
4364 255 246 120 6
Bpu10I CC'TnA_GC
Cuts at: 617 1891 617
Size: 1274 3717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 3046 3538 3836 2167
Size: 879 492 298 3322
Fragments arranged by size:
3322 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1140 4695 1140
Size: 3555 1436
BsaAI yAC'GTr
Cuts at: 215 876 2344 215
Size: 661 1468 2862
Fragments arranged by size:
2862 1468 661
BsaBI GATnn'nnATC
Cuts at: 6 1690 2505 4542 6
Size: 1684 815 2037 455
Fragments arranged by size:
2037 1684 815 455
BsaHI Gr'CG_yC
Cuts at: 812 1010 1592 1661 1833 2422 2670 3372 4868
Size: 198 582 69 172 589 248 702 1496
Cuts at: 4868 812
Size: 935
Fragments arranged by size:
1496 935 702 589 582 248 198 172
69
BsaWI w'CCGG_w
Cuts at: 2180 2701 3379 3653 3800 4211 2180
Size: 521 678 274 147 411 2960
Fragments arranged by size:
2960 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1474 1504 1474
Size: 30 4961
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 837 1000 1342 1863 1966 837
Size: 163 342 521 103 3862
Fragments arranged by size:
3862 521 342 163 103
BseYI C'CCAG_C
Cuts at: 438 630 645 663 750 1744 1857 2416 3174
Size: 192 15 18 87 994 113 559 758
Cuts at: 3174 3751 4247 438
Size: 577 496 1182
Fragments arranged by size:
1182 994 758 577 559 496 192 113
87 18 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 697 718 1411 1554 390
Size: 307 21 693 143 3827
Fragments arranged by size:
3827 693 307 143 21
BsiEI CG_ry'CG
Cuts at: 104 1250 2533 2579 3787 104
Size: 1146 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 1146 46
BsiHKAI G_wGCw'C
Cuts at: 503 1150 1352 1517 2005 2783 2973 3765 503
Size: 647 202 165 488 778 190 792 1729
Fragments arranged by size:
1729 792 778 647 488 202 190 165
BsmI GAATG_Cn'
Cuts at: 1038 1038
Size: 4991
BsmBI CGTCTCn'nnnn_
Cuts at: 225 1123 225
Size: 898 4093
Bsp1286I G_dGCh'C
Cuts at: 503 545 656 691 725 871 1067 1150 1352
Size: 42 111 35 34 146 196 83 202
Cuts at: 1352 1517 1626 1744 1809 1877 2005 2616 2709
Size: 165 109 118 65 68 128 611 93
Cuts at: 2709 2783 2973 3035 3765 503
Size: 74 190 62 730 1729
Fragments arranged by size:
1729 730 611 202 196 190 165 146
128 118 111 109 93 83 74 68
65 62 42 35 34
BspEI T'CCGG_A
Cuts at: 4211 4211
Size: 4991
BspHI T'CATG_A
Cuts at: 4167 4167
Size: 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 690 2041 2255 2557 2938 690
Size: 1351 214 302 381 2743
Fragments arranged by size:
2743 1351 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1479 3283 3337 4821 4986 50
Size: 1429 1804 54 1484 165 55
Fragments arranged by size:
1804 1484 1429 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1461 2903 1461
Size: 1442 3549
BsrFI r'CCGG_y
Cuts at: 668 1245 2989 3170 3379 668
Size: 577 1744 181 209 2280
Fragments arranged by size:
2280 1744 577 209 181
BssSI C'ACGA_G
Cuts at: 352 637 726 1510 3262 3620 352
Size: 285 89 784 1752 358 1723
Fragments arranged by size:
1752 1723 784 358 285 89
BstAPI GCAn_nnn'nTGC
Cuts at: 720 2368 720
Size: 1648 3343
BstBI TT'CG_AA
Cuts at: 206 3352 206
Size: 3146 1845
BstEII G'GTnAC_C
Cuts at: 990 990
Size: 4991
BstXI CCAn_nnnn'nTGG
Cuts at: 537 1602 537
Size: 1065 3926
BstYI r'GATC_y
Cuts at: 1 1199 1840 2016 2483 2506 2841 3087 4099
Size: 1198 641 176 467 23 335 246 1012
Cuts at: 4099 4185 4197 4543 1
Size: 86 12 346 449
Fragments arranged by size:
1198 1012 641 467 449 346 335 246
176 86 23 12
BtgI C'CryG_G
Cuts at: 459 1058 1208 1430 1664 459
Size: 599 150 222 234 3786
Fragments arranged by size:
3786 599 234 222 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1433 2941 3122 3285 1433
Size: 1508 181 163 3139
Fragments arranged by size:
3139 1508 181 163
BtsI GCAGTG_nn'
Cuts at: 124 706 1365 1399 2234 124
Size: 582 659 34 835 2881
Fragments arranged by size:
2881 835 659 582 34
DraI TTT'AAA
Cuts at: 1990 1990
Size: 4991
DraIII CAC_nnn'GTG
Cuts at: 720 720
Size: 4991
DrdI GACnn_nn'nnGTC
Cuts at: 940 2697 3555 940
Size: 1757 858 2376
Fragments arranged by size:
2376 1757 858
EaeI y'GGCC_r
Cuts at: 741 959 1025 1041 1115 1247 1302 2576 2750
Size: 218 66 16 74 132 55 1274 174
Cuts at: 2750 3141 3168 4513 741
Size: 391 27 1345 1219
Fragments arranged by size:
1345 1274 1219 391 218 174 132 74
66 55 27 16
EagI C'GGCC_G
Cuts at: 1247 2576 1247
Size: 1329 3662
EarI CTCTTCn'nnn_
Cuts at: 3014 3224 3014
Size: 210 4781
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3199 3509 3655 4801 3199
Size: 310 146 1146 3389
Fragments arranged by size:
3389 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2003 2003
Size: 4991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 997 1003 1258 1378 1624 2816 3248 3995 4263
Size: 6 255 120 246 1192 432 747 268
Cuts at: 4263 997
Size: 1725
Fragments arranged by size:
1725 1192 747 432 268 255 246 120
6
EcoNI CCTnn'n_nnAGG
Cuts at: 949 949
Size: 4991
EcoO109I rG'GnC_Cy
Cuts at: 387 465 1551 2130 387
Size: 78 1086 579 3248
Fragments arranged by size:
3248 1086 579 78
EcoRI G'AATT_C
Cuts at: 1995 1995
Size: 4991
EcoRV GAT'ATC
Cuts at: 516 516
Size: 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 471 3010 471
Size: 2539 2452
FspI TGC'GCA
Cuts at: 2772 2772
Size: 4991
HaeII r_GCGC'y
Cuts at: 1595 1664 1836 2425 2673 3695 4674 1595
Size: 69 172 589 248 1022 979 1912
Fragments arranged by size:
1912 1022 979 589 248 172 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1432 1464 2833 2863 2865 2895 1432
Size: 32 1369 30 2 30 3528
Fragments arranged by size:
3528 1369 32 30 30 2
HincII GTy'rAC
Cuts at: 522 2030 522
Size: 1508 3483
KasI G'GCGC_C
Cuts at: 1591 1660 1832 2421 2669 1591
Size: 69 172 589 248 3913
Fragments arranged by size:
3913 589 248 172 69
KpnI G_GTAC'C
Cuts at: 2011 2011
Size: 4991
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1439 3662 3846 1439
Size: 2223 184 2584
Fragments arranged by size:
2584 2223 184
MscI TGG'CCA
Cuts at: 1043 1304 2752 1043
Size: 261 1448 3282
Fragments arranged by size:
3282 1448 261
MslI CAynn'nnrTG
Cuts at: 173 538 568 694 2980 3107 173
Size: 365 30 126 2286 127 2057
Fragments arranged by size:
2286 2057 365 127 126 30
MspA1I CmG'CkG
Cuts at: 265 581 658 915 1236 1505 2102 2416 2776
Size: 316 77 257 321 269 597 314 360
Cuts at: 2776 3789 4034 265
Size: 1013 245 1222
Fragments arranged by size:
1222 1013 597 360 321 316 314 269
257 245 77
NaeI GCC'GGC
Cuts at: 670 1247 3172 670
Size: 577 1925 2489
Fragments arranged by size:
2489 1925 577
NarI GG'CG_CC
Cuts at: 1592 1661 1833 2422 2670 1592
Size: 69 172 589 248 3913
Fragments arranged by size:
3913 589 248 172 69
NgoMIV G'CCGG_C
Cuts at: 668 1245 3170 668
Size: 577 1925 2489
Fragments arranged by size:
2489 1925 577
NheI G'CTAG_C
Cuts at: 1956 1956
Size: 4991
NspI r_CATG'y
Cuts at: 2044 2544 3451 2044
Size: 500 907 3584
Fragments arranged by size:
3584 907 500
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4991
PflMI CCAn_nnn'nTGG
Cuts at: 750 750
Size: 4991
PfoI T'CCnGG_A
Cuts at: 245 509 947 974 1355 245
Size: 264 438 27 381 3881
Fragments arranged by size:
3881 438 381 264 27
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PmlI CAC'GTG
Cuts at: 215 876 215
Size: 661 4330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1468 1500 4130 4162 1468
Size: 32 2630 32 2297
Fragments arranged by size:
2630 2297 32 32
PpuMI rG'GwC_Cy
Cuts at: 387 465 387
Size: 78 4913
PstI C_TGCA'G
Cuts at: 788 1235 2038 2723 788
Size: 447 803 685 3056
Fragments arranged by size:
3056 803 685 447
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 581 1236 2416 2776 581
Size: 655 1180 360 2796
Fragments arranged by size:
2796 1180 655 360
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 2005 2005
Size: 4991
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 3014 3224 3014
Size: 210 4781
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 451 1276 4508 451
Size: 825 3232 934
Fragments arranged by size:
3232 934 825
SfcI C'TryA_G
Cuts at: 33 784 1231 2034 2719 3712 3903 33
Size: 751 447 803 685 993 191 1121
Fragments arranged by size:
1121 993 803 751 685 447 191
SfoI GGC'GCC
Cuts at: 1593 1662 1834 2423 2671 1593
Size: 69 172 589 248 3913
Fragments arranged by size:
3913 589 248 172 69
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 2013 2013
Size: 4991
SmlI C'TyrA_G
Cuts at: 2146 3061 3553 3815 2146
Size: 915 492 262 3322
Fragments arranged by size:
3322 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4991
SphI G_CATG'C
Cuts at: 2044 2544 2044
Size: 500 4491
StuI AGG'CCT
Cuts at: 763 763
Size: 4991
StyI C'CwwG_G
Cuts at: 1928 2125 2479 1928
Size: 197 354 4440
Fragments arranged by size:
4440 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 684 2574 2707 3166 3375 355
Size: 329 1890 133 459 209 1971
Fragments arranged by size:
1971 1890 459 329 209 133
TatI w'GTAC_w
Cuts at: 449 485 1274 1490 4506 449
Size: 36 789 216 3016 934
Fragments arranged by size:
3016 934 789 216 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4508 4540 4508
Size: 32 4959
Tth111I GACn'n_nGTC
Cuts at: 1009 2788 1009
Size: 1779 3212
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2486 2486
Size: 4991
XmaI C'CCGG_G
Cuts at: 2011 2011
Size: 4991
ZraI GAC'GTC
Cuts at: 813 813
Size: 4991
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI BsiWI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NotI NruI NsiI PacI PasI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI