Restriction map for
pF1KB8377
(Circular) (Six-base) MAPSORT of: pF1KB8377.seq Check: 8727 from: 1 to: 4979
pF1KB8377 4979 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 337 1154 1484 1529 1775 337
Size: 817 330 45 246 3541
Fragments arranged by size:
3541 817 330 246 45
AccI GT'mk_AC
Cuts at: 2017 2017
Size: 4979
Acc65I G'GTAC_C
Cuts at: 1995 1995
Size: 4979
AclI AA'CG_TT
Cuts at: 4819 4819
Size: 4979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 1756 1801 2804 3236 3983 4251 1465
Size: 291 45 1003 432 747 268 2193
Fragments arranged by size:
2193 1003 747 432 291 268 45
AflIII A'CryG_T
Cuts at: 2331 3435 4412 2331
Size: 1104 977 2898
Fragments arranged by size:
2898 1104 977
AgeI A'CCGG_T
Cuts at: 3367 3367
Size: 4979
AleI CACnn'nnGTG
Cuts at: 127 1818 127
Size: 1691 3288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1182 1182
Size: 4979
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1234 1266 1234
Size: 32 4947
AlwNI CAG_nnn'CTG
Cuts at: 765 984 1779 2284 3851 765
Size: 219 795 505 1567 1893
Fragments arranged by size:
1893 1567 795 505 219
ApaI G_GGCC'C
Cuts at: 443 1280 443
Size: 837 4142
ApaLI G'TGCA_C
Cuts at: 3749 3749
Size: 4979
ApoI r'AATT_y
Cuts at: 1531 1939 1983 4332 4588 1531
Size: 408 44 2349 256 1922
Fragments arranged by size:
2349 1922 408 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
AvaI C'yCGr_G
Cuts at: 1546 1999 1546
Size: 453 4526
BamHI G'GATC_C
Cuts at: 1904 2004 1904
Size: 100 4879
BanI G'GyrC_C
Cuts at: 1166 1507 1541 1995 2409 2657 2692 1166
Size: 341 34 454 414 248 35 3453
Fragments arranged by size:
3453 454 414 341 248 35 34
BanII G_rGCy'C
Cuts at: 136 257 266 287 443 461 1280 1338 1993
Size: 121 9 21 156 18 819 58 655
Cuts at: 1993 3023 136
Size: 1030 2092
Fragments arranged by size:
2092 1030 819 655 156 121 58 21
18 9
BbeI G_GCGC'C
Cuts at: 2413 2661 2413
Size: 248 4731
BbvCI CC'TCA_GC
Cuts at: 671 671
Size: 4979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1527 1561 1624 1658 1527
Size: 34 63 34 4848
Fragments arranged by size:
4848 63 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2877 3638 4979 1689
Size: 1188 761 1341 1689
Fragments arranged by size:
1689 1341 1188 761
BclI T'GATC_A
Cuts at: 767 2499 767
Size: 1732 3247
BglI GCCn_nnn'nGGC
Cuts at: 193 536 193
Size: 343 4636
BglII A'GATC_T
Cuts at: 2494 4531 2494
Size: 2037 2942
BlpI GC'TnA_GC
Cuts at: 1369 2091 1369
Size: 722 4257
Bme1580I G_kGCm'C
Cuts at: 443 1280 1512 1741 2604 2697 3753 443
Size: 837 232 229 863 93 1056 1669
Fragments arranged by size:
1669 1056 863 837 232 229 93
BmrI ACTGGGnnnn_n'
Cuts at: 871 994 1513 2191 2272 2607 4751 871
Size: 123 519 678 81 335 2144 1099
Fragments arranged by size:
2144 1099 678 519 335 123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 283 3346 283
Size: 3063 1916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 439 742 979 1027 1036 1256 1849 120
Size: 319 303 237 48 9 220 593 3250
Fragments arranged by size:
3250 593 319 303 237 220 48 9
Bpu10I CC'TnA_GC
Cuts at: 236 460 671 236
Size: 224 211 4544
Fragments arranged by size:
4544 224 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 3034 3526 3824 2155
Size: 879 492 298 3310
Fragments arranged by size:
3310 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 592 760 1467 1874 4683 592
Size: 168 707 407 2809 888
Fragments arranged by size:
2809 888 707 407 168
BsaAI yAC'GTr
Cuts at: 2332 2332
Size: 4979
BsaBI GATnn'nnATC
Cuts at: 6 2493 4530 6
Size: 2487 2037 455
Fragments arranged by size:
2487 2037 455
BsaHI Gr'CG_yC
Cuts at: 1925 2410 2658 3360 4856 1925
Size: 485 248 702 1496 2048
Fragments arranged by size:
2048 1496 702 485 248
BsaWI w'CCGG_w
Cuts at: 205 2168 2689 3367 3641 3788 4199 205
Size: 1963 521 678 274 147 411 985
Fragments arranged by size:
1963 985 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 1234 1264 499
Size: 30 705 30 4214
Fragments arranged by size:
4214 705 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 1375 1711 256
Size: 1119 336 3524
Fragments arranged by size:
3524 1119 336
BseYI C'CCAG_C
Cuts at: 666 2404 3162 3739 4235 666
Size: 1738 758 577 496 1410
Fragments arranged by size:
1738 1410 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1750 1796 508
Size: 1242 46 3691
Fragments arranged by size:
3691 1242 46
BsiEI CG_ry'CG
Cuts at: 104 2521 2567 3775 104
Size: 2417 46 1208 1308
Fragments arranged by size:
2417 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 903 1993 2771 2961 3753 903
Size: 1090 778 190 792 2129
Fragments arranged by size:
2129 1090 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1184 1184
Size: 4979
Bsp1286I G_dGCh'C
Cuts at: 136 257 266 287 443 461 903 1280 1338
Size: 121 9 21 156 18 442 377 58
Cuts at: 1338 1512 1741 1993 2604 2697 2771 2961 3023
Size: 174 229 252 611 93 74 190 62
Cuts at: 3023 3753 136
Size: 730 1362
Fragments arranged by size:
1362 730 611 442 377 252 229 190
174 156 121 93 74 62 58 21
18 9
BspEI T'CCGG_A
Cuts at: 4199 4199
Size: 4979
BspHI T'CATG_A
Cuts at: 804 4155 804
Size: 3351 1628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 1154 1223 1484 1529 1775 2029 2243 2545
Size: 817 69 261 45 246 254 214 302
Cuts at: 2545 2926 337
Size: 381 2390
Fragments arranged by size:
2390 817 381 302 261 254 246 214
69 45
BsrBI CCG'CTC
Cuts at: 50 3271 3325 4809 4974 50
Size: 3221 54 1484 165 55
Fragments arranged by size:
3221 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2891 2891
Size: 4979
BsrFI r'CCGG_y
Cuts at: 170 2977 3158 3367 170
Size: 2807 181 209 1782
Fragments arranged by size:
2807 1782 209 181
BsrGI T'GTAC_A
Cuts at: 851 851
Size: 4979
BssSI C'ACGA_G
Cuts at: 3250 3608 3250
Size: 358 4621
BstAPI GCAn_nnn'nTGC
Cuts at: 2356 2356
Size: 4979
BstBI TT'CG_AA
Cuts at: 3340 3340
Size: 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 129 144 129
Size: 15 4964
BstYI r'GATC_y
Cuts at: 1 633 1904 2004 2471 2494 2829 3075 4087
Size: 632 1271 100 467 23 335 246 1012
Cuts at: 4087 4173 4185 4531 1
Size: 86 12 346 449
Fragments arranged by size:
1271 1012 632 467 449 346 335 246
100 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 346 816 1289 1571 346
Size: 470 473 282 3754
Fragments arranged by size:
3754 473 470 282
BtgI C'CryG_G
Cuts at: 829 829
Size: 4979
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2929 3110 3273 2929
Size: 181 163 4635
Fragments arranged by size:
4635 181 163
BtsI GCAGTG_nn'
Cuts at: 1630 1808 2222 1630
Size: 178 414 4387
Fragments arranged by size:
4387 414 178
DraI TTT'AAA
Cuts at: 1978 1978
Size: 4979
DrdI GACnn_nn'nnGTC
Cuts at: 2685 3543 2685
Size: 858 4121
EaeI y'GGCC_r
Cuts at: 1689 2564 2738 3129 3156 4501 1689
Size: 875 174 391 27 1345 2167
Fragments arranged by size:
2167 1345 875 391 174 27
EagI C'GGCC_G
Cuts at: 2564 2564
Size: 4979
EarI CTCTTCn'nnn_
Cuts at: 1561 1777 3002 3212 1561
Size: 216 1225 210 3328
Fragments arranged by size:
3328 1225 216 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3187 3497 3643 4789 3187
Size: 310 146 1146 3377
Fragments arranged by size:
3377 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1991 1991
Size: 4979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 439 742 979 1027 1036 1256 1465 1756
Size: 319 303 237 48 9 220 209 291
Cuts at: 1756 1801 1849 2804 3236 3983 4251 120
Size: 45 48 955 432 747 268 848
Fragments arranged by size:
955 848 747 432 319 303 291 268
237 220 209 48 48 45 9
EcoNI CCTnn'n_nnAGG
Cuts at: 928 1480 928
Size: 552 4427
EcoO109I rG'GnC_Cy
Cuts at: 439 579 866 946 1276 1277 2118 439
Size: 140 287 80 330 1 841 3300
Fragments arranged by size:
3300 841 330 287 140 80 1
EcoRI G'AATT_C
Cuts at: 1983 1983
Size: 4979
EcoRV GAT'ATC
Cuts at: 393 393
Size: 4979
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 4979
FspI TGC'GCA
Cuts at: 2760 2760
Size: 4979
HaeII r_GCGC'y
Cuts at: 2413 2661 3683 4662 2413
Size: 248 1022 979 2730
Fragments arranged by size:
2730 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 499 531 873 905 1096 1128 1776 1808 2821
Size: 32 342 32 191 32 648 32 1013
Cuts at: 2821 2851 2853 2883 499
Size: 30 2 30 2595
Fragments arranged by size:
2595 1013 648 342 191 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2018 2018
Size: 4979
HindIII A'AGCT_T
Cuts at: 354 354
Size: 4979
KasI G'GCGC_C
Cuts at: 2409 2657 2409
Size: 248 4731
KpnI G_GTAC'C
Cuts at: 1999 1999
Size: 4979
MluI A'CGCG_T
Cuts at: 4412 4412
Size: 4979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 428 3650 3834 428
Size: 3222 184 1573
Fragments arranged by size:
3222 1573 184
MscI TGG'CCA
Cuts at: 2740 2740
Size: 4979
MslI CAynn'nnrTG
Cuts at: 127 1818 1856 2968 3095 127
Size: 1691 38 1112 127 2011
Fragments arranged by size:
2011 1691 1112 127 38
MspA1I CmG'CkG
Cuts at: 140 295 399 670 1309 1688 2090 2404 2764
Size: 155 104 271 639 379 402 314 360
Cuts at: 2764 3777 4022 140
Size: 1013 245 1097
Fragments arranged by size:
1097 1013 639 402 379 360 314 271
245 155 104
NaeI GCC'GGC
Cuts at: 3160 3160
Size: 4979
NarI GG'CG_CC
Cuts at: 2410 2658 2410
Size: 248 4731
NgoMIV G'CCGG_C
Cuts at: 3158 3158
Size: 4979
NspI r_CATG'y
Cuts at: 2032 2532 3439 2032
Size: 500 907 3572
Fragments arranged by size:
3572 907 500
PasI CC'CwG_GG
Cuts at: 363 708 363
Size: 345 4634
PciI A'CATG_T
Cuts at: 3435 3435
Size: 4979
PflMI CCAn_nnn'nTGG
Cuts at: 1518 1518
Size: 4979
PfoI T'CCnGG_A
Cuts at: 758 758
Size: 4979
PmeI GTTT'AAAC
Cuts at: 1978 1978
Size: 4979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 443 475 1234 1266 4118 4150 443
Size: 32 759 32 2852 32 1272
Fragments arranged by size:
2852 1272 759 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 579 866 946 579
Size: 287 80 4612
Fragments arranged by size:
4612 287 80
PspOMI G'GGCC_C
Cuts at: 439 1276 439
Size: 837 4142
PspXI vC'TCGA_Gb
Cuts at: 1546 1546
Size: 4979
PstI C_TGCA'G
Cuts at: 294 545 701 1200 1637 2026 2711 294
Size: 251 156 499 437 389 685 2562
Fragments arranged by size:
2562 685 499 437 389 251 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4979
PvuII CAG'CTG
Cuts at: 140 295 670 2404 2764 140
Size: 155 375 1734 360 2355
Fragments arranged by size:
2355 1734 375 360 155
RsrII CG'GwC_CG
Cuts at: 3174 3174
Size: 4979
SacI G_AGCT'C
Cuts at: 1993 1993
Size: 4979
SalI G'TCGA_C
Cuts at: 2016 2016
Size: 4979
SapI GCTCTTCn'nnn_
Cuts at: 1561 3002 3212 1561
Size: 1441 210 3328
Fragments arranged by size:
3328 1441 210
SbfI CC_TGCA'GG
Cuts at: 545 701 2026 545
Size: 156 1325 3498
Fragments arranged by size:
3498 1325 156
ScaI AGT'ACT
Cuts at: 4496 4496
Size: 4979
SexAI A'CCwGG_T
Cuts at: 1970 1970
Size: 4979
SfcI C'TryA_G
Cuts at: 33 290 541 697 1171 1196 1633 2022 2707
Size: 257 251 156 474 25 437 389 685
Cuts at: 2707 3700 3891 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 474 437 389 257 251
191 156 25
SfoI GGC'GCC
Cuts at: 2411 2659 2411
Size: 248 4731
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4979
SmaI CCC'GGG
Cuts at: 2001 2001
Size: 4979
SmlI C'TyrA_G
Cuts at: 1546 2134 3049 3541 3803 1546
Size: 588 915 492 262 2722
Fragments arranged by size:
2722 915 588 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4979
SphI G_CATG'C
Cuts at: 2032 2532 2032
Size: 500 4479
StuI AGG'CCT
Cuts at: 821 964 1033 1492 821
Size: 143 69 459 4308
Fragments arranged by size:
4308 459 143 69
StyI C'CwwG_G
Cuts at: 188 311 750 1246 1433 2113 2467 188
Size: 123 439 496 187 680 354 2700
Fragments arranged by size:
2700 680 496 439 354 187 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1486 1495 2562 2695 3154 3363 1486
Size: 9 1067 133 459 209 3102
Fragments arranged by size:
3102 1067 459 209 133 9
TatI w'GTAC_w
Cuts at: 851 908 4494 851
Size: 57 3586 1336
Fragments arranged by size:
3586 1336 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4496 4528 4496
Size: 32 4947
Tth111I GACn'n_nGTC
Cuts at: 478 2776 478
Size: 2298 2681
XbaI T'CTAG_A
Cuts at: 2010 2010
Size: 4979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2474 2474
Size: 4979
XhoI C'TCGA_G
Cuts at: 1546 1546
Size: 4979
XmaI C'CCGG_G
Cuts at: 1999 1999
Size: 4979
XmnI GAAnn'nnTTC
Cuts at: 1940 1940
Size: 4979
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BaeI BbsI
BfrBI BmgBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
ClaI CspCI DraIII FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI