Restriction map for
pF1KB8379
(Circular) (Six-base) MAPSORT of: pF1KB8379.seq Check: 3105 from: 1 to: 4955
pF1KB8379 4955 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 845 1164 845
Size: 319 4636
AccI GT'mk_AC
Cuts at: 380 1475 1993 380
Size: 1095 518 3342
Fragments arranged by size:
3342 1095 518
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 1221 2780 3212 3959 4227 286
Size: 935 1559 432 747 268 1014
Fragments arranged by size:
1559 1014 935 747 432 268
AflIII A'CryG_T
Cuts at: 500 593 2307 3411 4388 500
Size: 93 1714 1104 977 1067
Fragments arranged by size:
1714 1104 1067 977 93
AgeI A'CCGG_T
Cuts at: 3343 3343
Size: 4955
AhdI GACnn_n'nnGTC
Cuts at: 1251 1251
Size: 4955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 853 905 853
Size: 52 4903
AlwNI CAG_nnn'CTG
Cuts at: 126 732 1041 1179 1626 2260 3827 126
Size: 606 309 138 447 634 1567 1254
Fragments arranged by size:
1567 1254 634 606 447 309 138
ApaI G_GGCC'C
Cuts at: 283 1230 283
Size: 947 4008
ApaLI G'TGCA_C
Cuts at: 1894 3725 1894
Size: 1831 3124
ApoI r'AATT_y
Cuts at: 1959 4308 4564 1959
Size: 2349 256 2350
Fragments arranged by size:
2350 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 274 702 1363 1469 1676 1975 274
Size: 428 661 106 207 299 3254
Fragments arranged by size:
3254 661 428 299 207 106
BamHI G'GATC_C
Cuts at: 1568 1662 1980 1568
Size: 94 318 4543
Fragments arranged by size:
4543 318 94
BanI G'GyrC_C
Cuts at: 445 889 1465 1533 1971 2385 2633 2668 445
Size: 444 576 68 438 414 248 35 2732
Fragments arranged by size:
2732 576 444 438 414 248 68 35
BanII G_rGCy'C
Cuts at: 283 356 1207 1230 1676 1722 1885 1969 2999
Size: 73 851 23 446 46 163 84 1030
Cuts at: 2999 283
Size: 2239
Fragments arranged by size:
2239 1030 851 446 163 84 73 46
23
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BbsI GAAGACnn'nnnn_
Cuts at: 274 1074 274
Size: 800 4155
BbvCI CC'TCA_GC
Cuts at: 200 200
Size: 4955
BciVI GTATCCnnnnn_n'
Cuts at: 2853 3614 4955 2853
Size: 761 1341 2853
Fragments arranged by size:
2853 1341 761
BclI T'GATC_A
Cuts at: 2475 2475
Size: 4955
BglI GCCn_nnn'nGGC
Cuts at: 1441 1441
Size: 4955
BglII A'GATC_T
Cuts at: 2470 4507 2470
Size: 2037 2918
BlpI GC'TnA_GC
Cuts at: 475 1721 1819 2067 475
Size: 1246 98 248 3363
Fragments arranged by size:
3363 1246 248 98
Bme1580I G_kGCm'C
Cuts at: 283 448 894 1230 1429 1470 1538 1898 2580
Size: 165 446 336 199 41 68 360 682
Cuts at: 2580 2673 3729 283
Size: 93 1056 1509
Fragments arranged by size:
1509 1056 682 446 360 336 199 165
93 68 41
BmgBI CAC'GTC
Cuts at: 594 594
Size: 4955
BmrI ACTGGGnnnn_n'
Cuts at: 1647 2167 2248 2583 4727 1647
Size: 520 81 335 2144 1875
Fragments arranged by size:
2144 1875 520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1138 3322 1138
Size: 2184 2771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 959 1774 1899 959
Size: 815 125 4015
Fragments arranged by size:
4015 815 125
Bpu10I CC'TnA_GC
Cuts at: 200 1393 200
Size: 1193 3762
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 2131 3010 3502 3800 1055
Size: 1076 879 492 298 2210
Fragments arranged by size:
2210 1076 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 721 1673 4659 721
Size: 952 2986 1017
Fragments arranged by size:
2986 1017 952
BsaAI yAC'GTr
Cuts at: 1062 2308 1062
Size: 1246 3709
BsaBI GATnn'nnATC
Cuts at: 6 2469 4506 6
Size: 2463 2037 455
Fragments arranged by size:
2463 2037 455
BsaHI Gr'CG_yC
Cuts at: 2386 2634 3336 4832 2386
Size: 248 702 1496 2509
Fragments arranged by size:
2509 1496 702 248
BsaWI w'CCGG_w
Cuts at: 348 1085 2144 2665 3343 3617 3764 4175 348
Size: 737 1059 521 678 274 147 411 1128
Fragments arranged by size:
1128 1059 737 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 176 1840 1870 146
Size: 30 1664 30 3231
Fragments arranged by size:
3231 1664 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 157 160 203 573 1618 154
Size: 3 3 43 370 1045 3491
Fragments arranged by size:
3491 1045 370 43 3 3
BseYI C'CCAG_C
Cuts at: 850 1436 1444 1619 2380 3138 3715 4211 850
Size: 586 8 175 761 758 577 496 1594
Fragments arranged by size:
1594 761 758 586 577 496 175 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 769 115
Size: 654 4301
BsiEI CG_ry'CG
Cuts at: 104 1750 2497 2543 3751 104
Size: 1646 747 46 1208 1308
Fragments arranged by size:
1646 1308 1208 747 46
BsiHKAI G_wGCw'C
Cuts at: 1885 1898 1969 2747 2937 3729 1885
Size: 13 71 778 190 792 3111
Fragments arranged by size:
3111 792 778 190 71 13
BsmI GAATG_Cn'
Cuts at: 257 257
Size: 4955
Bsp1286I G_dGCh'C
Cuts at: 283 356 448 894 1207 1230 1429 1470 1538
Size: 73 92 446 313 23 199 41 68
Cuts at: 1538 1676 1722 1885 1898 1969 2580 2673 2747
Size: 138 46 163 13 71 611 93 74
Cuts at: 2747 2937 2999 3729 283
Size: 190 62 730 1509
Fragments arranged by size:
1509 730 611 446 313 199 190 163
138 93 92 74 73 71 68 62
46 41 23 13
BspEI T'CCGG_A
Cuts at: 4175 4175
Size: 4955
BspHI T'CATG_A
Cuts at: 4131 4131
Size: 4955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 845 1026 1164 1751 2005 2219 2521 2902 845
Size: 181 138 587 254 214 302 381 2898
Fragments arranged by size:
2898 587 381 302 254 214 181 138
BsrBI CCG'CTC
Cuts at: 50 887 3247 3301 4785 4950 50
Size: 837 2360 54 1484 165 55
Fragments arranged by size:
2360 1484 837 165 55 54
BsrDI GCAATG_nn'
Cuts at: 714 2867 714
Size: 2153 2802
BsrFI r'CCGG_y
Cuts at: 245 2953 3134 3343 245
Size: 2708 181 209 1857
Fragments arranged by size:
2708 1857 209 181
BssHII G'CGCG_C
Cuts at: 1773 1773
Size: 4955
BssSI C'ACGA_G
Cuts at: 3226 3584 3226
Size: 358 4597
BstAPI GCAn_nnn'nTGC
Cuts at: 126 747 1065 2332 126
Size: 621 318 1267 2749
Fragments arranged by size:
2749 1267 621 318
BstBI TT'CG_AA
Cuts at: 3316 3316
Size: 4955
BstEII G'GTnAC_C
Cuts at: 775 775
Size: 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 951 1444 951
Size: 493 4462
BstYI r'GATC_y
Cuts at: 1 551 1568 1662 1980 2447 2470 2805 3051
Size: 550 1017 94 318 467 23 335 246
Cuts at: 3051 4063 4149 4161 4507 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1017 1012 550 467 449 346 335 318
246 94 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1381 1381
Size: 4955
BtgI C'CryG_G
Cuts at: 323 911 1164 1430 1539 323
Size: 588 253 266 109 3739
Fragments arranged by size:
3739 588 266 253 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1412 2905 3086 3249 1412
Size: 1493 181 163 3118
Fragments arranged by size:
3118 1493 181 163
BtsI GCAGTG_nn'
Cuts at: 127 748 2198 127
Size: 621 1450 2884
Fragments arranged by size:
2884 1450 621
DraI TTT'AAA
Cuts at: 1954 1954
Size: 4955
DraIII CAC_nnn'GTG
Cuts at: 963 963
Size: 4955
DrdI GACnn_nn'nnGTC
Cuts at: 1417 2661 3519 1417
Size: 1244 858 2853
Fragments arranged by size:
2853 1244 858
EaeI y'GGCC_r
Cuts at: 603 1280 1747 2540 2714 3105 3132 4477 603
Size: 677 467 793 174 391 27 1345 1081
Fragments arranged by size:
1345 1081 793 677 467 391 174 27
EagI C'GGCC_G
Cuts at: 1747 2540 1747
Size: 793 4162
EarI CTCTTCn'nnn_
Cuts at: 2978 3188 2978
Size: 210 4745
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 3163 3473 3619 4765 1861
Size: 1302 310 146 1146 2051
Fragments arranged by size:
2051 1302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1883 1967 1883
Size: 84 4871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 959 1221 1774 1899 2780 3212 3959 4227
Size: 673 262 553 125 881 432 747 268
Cuts at: 4227 286
Size: 1014
Fragments arranged by size:
1014 881 747 673 553 432 268 262
125
EcoNI CCTnn'n_nnAGG
Cuts at: 368 1384 368
Size: 1016 3939
EcoO109I rG'GnC_Cy
Cuts at: 113 279 280 328 578 699 858 1038 1140
Size: 166 1 48 250 121 159 180 102
Cuts at: 1140 1226 1227 1368 1389 1689 2094 113
Size: 86 1 141 21 300 405 2974
Fragments arranged by size:
2974 405 300 250 180 166 159 141
121 102 86 48 21 1 1
EcoRI G'AATT_C
Cuts at: 1959 1959
Size: 4955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4955
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 1826 1854 2389 2637 3659 4638 1826
Size: 28 535 248 1022 979 2143
Fragments arranged by size:
2143 1022 979 535 248 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 769 801 1189 1221 1237 1269 1924 1956 2797
Size: 32 388 32 16 32 655 32 841
Cuts at: 2797 2827 2829 2859 769
Size: 30 2 30 2865
Fragments arranged by size:
2865 841 655 388 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 1994 1994
Size: 4955
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3626 3810 3626
Size: 184 4771
MscI TGG'CCA
Cuts at: 2716 2716
Size: 4955
MslI CAynn'nnrTG
Cuts at: 451 2944 3071 451
Size: 2493 127 2335
Fragments arranged by size:
2493 2335 127
MspA1I CmG'CkG
Cuts at: 951 1440 1752 1877 2066 2380 2740 3753 3998
Size: 489 312 125 189 314 360 1013 245
Cuts at: 3998 951
Size: 1908
Fragments arranged by size:
1908 1013 489 360 314 312 245 189
125
NaeI GCC'GGC
Cuts at: 247 3136 247
Size: 2889 2066
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NcoI C'CATG_G
Cuts at: 323 323
Size: 4955
NgoMIV G'CCGG_C
Cuts at: 245 3134 245
Size: 2889 2066
NotI GC'GGCC_GC
Cuts at: 1747 1747
Size: 4955
NspI r_CATG'y
Cuts at: 834 2008 2508 3415 834
Size: 1174 500 907 2374
Fragments arranged by size:
2374 1174 907 500
PasI CC'CwG_GG
Cuts at: 905 1461 905
Size: 556 4399
PciI A'CATG_T
Cuts at: 3411 3411
Size: 4955
PflMI CCAn_nnn'nTGG
Cuts at: 417 963 417
Size: 546 4409
PfoI T'CCnGG_A
Cuts at: 719 719
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PmlI CAC'GTG
Cuts at: 1062 1062
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1540 1572 4094 4126 1540
Size: 32 2522 32 2369
Fragments arranged by size:
2522 2369 32 32
PpuMI rG'GwC_Cy
Cuts at: 328 1038 1368 328
Size: 710 330 3915
Fragments arranged by size:
3915 710 330
PspOMI G'GGCC_C
Cuts at: 279 1226 279
Size: 947 4008
PstI C_TGCA'G
Cuts at: 743 884 1175 2002 2687 743
Size: 141 291 827 685 3011
Fragments arranged by size:
3011 827 685 291 141
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 951 1440 2380 2740 951
Size: 489 940 360 3166
Fragments arranged by size:
3166 940 489 360
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1885 1969 1885
Size: 84 4871
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SanDI GG'GwC_CC
Cuts at: 328 328
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 2978 3188 2978
Size: 210 4745
SbfI CC_TGCA'GG
Cuts at: 1175 2002 1175
Size: 827 4128
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SexAI A'CCwGG_T
Cuts at: 1946 1946
Size: 4955
SfcI C'TryA_G
Cuts at: 33 570 739 880 1171 1920 1998 2683 3676
Size: 537 169 141 291 749 78 685 993
Cuts at: 3676 3867 33
Size: 191 1121
Fragments arranged by size:
1121 993 749 685 537 291 191 169
141 78
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 276 704 1471 1977 276
Size: 428 767 506 3254
Fragments arranged by size:
3254 767 506 428
SmlI C'TyrA_G
Cuts at: 1070 2110 3025 3517 3779 1070
Size: 1040 915 492 262 2246
Fragments arranged by size:
2246 1040 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4955
SphI G_CATG'C
Cuts at: 834 2008 2508 834
Size: 1174 500 3281
Fragments arranged by size:
3281 1174 500
StuI AGG'CCT
Cuts at: 1783 1783
Size: 4955
StyI C'CwwG_G
Cuts at: 323 364 587 1685 2089 2443 323
Size: 41 223 1098 404 354 2835
Fragments arranged by size:
2835 1098 404 354 223 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 336 735 1174 2538 2671 3130 3339 172
Size: 164 399 439 1364 133 459 209 1788
Fragments arranged by size:
1788 1364 459 439 399 209 164 133
TatI w'GTAC_w
Cuts at: 4470 4470
Size: 4955
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 124 146 156 178 826 858 1486 1518 1647
Size: 22 10 22 648 32 628 32 129
Cuts at: 1647 1679 1840 1872 4472 4504 124
Size: 32 161 32 2600 32 575
Fragments arranged by size:
2600 648 628 575 161 129 32 32
32 32 32 22 22 10
Tth111I GACn'n_nGTC
Cuts at: 332 2752 332
Size: 2420 2535
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 918 2450 918
Size: 1532 3423
XmaI C'CCGG_G
Cuts at: 274 702 1469 1975 274
Size: 428 767 506 3254
Fragments arranged by size:
3254 767 506 428
XmnI GAAnn'nnTTC
Cuts at: 1612 1801 1909 1612
Size: 189 108 4658
Fragments arranged by size:
4658 189 108
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmBI BsrGI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI