Restriction map for
pF1KB8381
(Circular) (Six-base) MAPSORT of: pF1KB8381.seq Check: 5811 from: 1 to: 4619
pF1KB8381 4619 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1657 1657
Size: 4619
Acc65I G'GTAC_C
Cuts at: 1635 1635
Size: 4619
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 478 1630 2444 2876 3623 3891 142
Size: 336 1152 814 432 747 268 870
Fragments arranged by size:
1152 870 814 747 432 336 268
AflIII A'CryG_T
Cuts at: 1971 3075 4052 1971
Size: 1104 977 2538
Fragments arranged by size:
2538 1104 977
AgeI A'CCGG_T
Cuts at: 3007 3007
Size: 4619
AlwNI CAG_nnn'CTG
Cuts at: 204 1924 3491 204
Size: 1720 1567 1332
Fragments arranged by size:
1720 1567 1332
ApaLI G'TGCA_C
Cuts at: 673 3389 673
Size: 2716 1903
ApoI r'AATT_y
Cuts at: 395 551 1502 1623 3972 4228 395
Size: 156 951 121 2349 256 786
Fragments arranged by size:
2349 951 786 256 156 121
AseI AT'TA_AT
Cuts at: 20 440 20
Size: 420 4199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 949 1639 949
Size: 690 3929
AvrII C'CTAG_G
Cuts at: 595 595
Size: 4619
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 214 726 1635 2049 2297 2332 214
Size: 512 909 414 248 35 2501
Fragments arranged by size:
2501 909 512 414 248 35
BanII G_rGCy'C
Cuts at: 120 1633 2663 120
Size: 1513 1030 2076
Fragments arranged by size:
2076 1513 1030
BbeI G_GCGC'C
Cuts at: 2053 2301 2053
Size: 248 4371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1108 1142 1108
Size: 34 4585
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3278 4619 2517
Size: 761 1341 2517
Fragments arranged by size:
2517 1341 761
BclI T'GATC_A
Cuts at: 2139 2139
Size: 4619
BfrBI ATG'CAT
Cuts at: 1246 1246
Size: 4619
BglII A'GATC_T
Cuts at: 2134 4171 2134
Size: 2037 2582
BlpI GC'TnA_GC
Cuts at: 1106 1442 1731 1106
Size: 336 289 3994
Fragments arranged by size:
3994 336 289
Bme1580I G_kGCm'C
Cuts at: 677 2244 2337 3393 677
Size: 1567 93 1056 1903
Fragments arranged by size:
1903 1567 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 214 1831 1912 2247 4391 214
Size: 1617 81 335 2144 442
Fragments arranged by size:
2144 1617 442 335 81
BmtI G_CTAG'C
Cuts at: 182 182
Size: 4619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 293 2986 293
Size: 2693 1926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 546 563 1046 320
Size: 226 17 483 3893
Fragments arranged by size:
3893 483 226 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1795 2674 3166 3464 1795
Size: 879 492 298 2950
Fragments arranged by size:
2950 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4323 4323
Size: 4619
BsaAI yAC'GTr
Cuts at: 959 1972 959
Size: 1013 3606
BsaBI GATnn'nnATC
Cuts at: 6 1207 1595 2133 4170 6
Size: 1201 388 538 2037 455
Fragments arranged by size:
2037 1201 538 455 388
BsaHI Gr'CG_yC
Cuts at: 2050 2298 3000 4496 2050
Size: 248 702 1496 2173
Fragments arranged by size:
2173 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1808 2329 3007 3281 3428 3839 1808
Size: 521 678 274 147 411 2588
Fragments arranged by size:
2588 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 619 649 619
Size: 30 4589
BseYI C'CCAG_C
Cuts at: 2044 2802 3379 3875 2044
Size: 758 577 496 2788
Fragments arranged by size:
2788 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 854 184
Size: 670 3949
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3415 104
Size: 2057 46 1208 1308
Fragments arranged by size:
2057 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 677 788 1514 1633 2411 2601 3393 677
Size: 111 726 119 778 190 792 1903
Fragments arranged by size:
1903 792 778 726 190 119 111
BsmI GAATG_Cn'
Cuts at: 407 407
Size: 4619
Bsp1286I G_dGCh'C
Cuts at: 120 677 788 1514 1633 2244 2337 2411 2601
Size: 557 111 726 119 611 93 74 190
Cuts at: 2601 2663 3393 120
Size: 62 730 1346
Fragments arranged by size:
1346 730 726 611 557 190 119 111
93 74 62
BspEI T'CCGG_A
Cuts at: 3839 3839
Size: 4619
BspHI T'CATG_A
Cuts at: 885 3795 885
Size: 2910 1709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1669 1883 2185 2566 1669
Size: 214 302 381 3722
Fragments arranged by size:
3722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 241 2531 241
Size: 2290 2329
BsrFI r'CCGG_y
Cuts at: 2617 2798 3007 2617
Size: 181 209 4229
Fragments arranged by size:
4229 209 181
BssSI C'ACGA_G
Cuts at: 2890 3248 2890
Size: 358 4261
BstAPI GCAn_nnn'nTGC
Cuts at: 1116 1996 1116
Size: 880 3739
BstBI TT'CG_AA
Cuts at: 399 2980 399
Size: 2581 2038
BstXI CCAn_nnnn'nTGG
Cuts at: 1213 1213
Size: 4619
BstYI r'GATC_y
Cuts at: 1 1644 2111 2134 2469 2715 3727 3813 3825
Size: 1643 467 23 335 246 1012 86 12
Cuts at: 3825 4171 1
Size: 346 449
Fragments arranged by size:
1643 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1097 1097
Size: 4619
BtgI C'CryG_G
Cuts at: 107 943 107
Size: 836 3783
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 347 1117 1300 1862 347
Size: 770 183 562 3104
Fragments arranged by size:
3104 770 562 183
ClaI AT'CG_AT
Cuts at: 590 590
Size: 4619
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DraIII CAC_nnn'GTG
Cuts at: 935 935
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3183 2325
Size: 858 3761
EaeI y'GGCC_r
Cuts at: 134 195 797 2204 2378 2769 2796 4141 134
Size: 61 602 1407 174 391 27 1345 612
Fragments arranged by size:
1407 1345 612 602 391 174 61 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 257 451 2642 2852 257
Size: 194 2191 210 2024
Fragments arranged by size:
2191 2024 210 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2827 3137 3283 4429 2827
Size: 310 146 1146 3017
Fragments arranged by size:
3017 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1631 1631
Size: 4619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 320 478 546 563 1046 1630 2444 2876
Size: 178 158 68 17 483 584 814 432
Cuts at: 2876 3623 3891 142
Size: 747 268 870
Fragments arranged by size:
870 814 747 584 483 432 268 178
158 68 17
EcoO109I rG'GnC_Cy
Cuts at: 639 1758 639
Size: 1119 3500
EcoRI G'AATT_C
Cuts at: 551 1502 1623 551
Size: 951 121 3547
Fragments arranged by size:
3547 951 121
EcoRV GAT'ATC
Cuts at: 567 567
Size: 4619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4619
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 2053 2301 3323 4302 2053
Size: 248 1022 979 2370
Fragments arranged by size:
2370 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1251 1283 1348 1380 2461 2491 2493 2523 1251
Size: 32 65 32 1081 30 2 30 3347
Fragments arranged by size:
3347 1081 65 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1277 1658 1277
Size: 381 4238
HpaI GTT'AAC
Cuts at: 1277 1277
Size: 4619
KasI G'GCGC_C
Cuts at: 2049 2297 2049
Size: 248 4371
KpnI G_GTAC'C
Cuts at: 1639 1639
Size: 4619
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 631 3290 3474 631
Size: 2659 184 1776
Fragments arranged by size:
2659 1776 184
MscI TGG'CCA
Cuts at: 2380 2380
Size: 4619
MslI CAynn'nnrTG
Cuts at: 1355 2608 2735 1355
Size: 1253 127 3239
Fragments arranged by size:
3239 1253 127
MspA1I CmG'CkG
Cuts at: 803 1417 1730 2044 2404 3417 3662 803
Size: 614 313 314 360 1013 245 1760
Fragments arranged by size:
1760 1013 614 360 314 313 245
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 2050 2298 2050
Size: 248 4371
NcoI C'CATG_G
Cuts at: 107 943 107
Size: 836 3783
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NheI G'CTAG_C
Cuts at: 178 178
Size: 4619
NsiI A_TGCA'T
Cuts at: 1248 1248
Size: 4619
NspI r_CATG'y
Cuts at: 1556 1672 2172 3079 1556
Size: 116 500 907 3096
Fragments arranged by size:
3096 907 500 116
PciI A'CATG_T
Cuts at: 3075 3075
Size: 4619
PflMI CCAn_nnn'nTGG
Cuts at: 113 204 982 113
Size: 91 778 3750
Fragments arranged by size:
3750 778 91
PfoI T'CCnGG_A
Cuts at: 187 187
Size: 4619
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4587
PpuMI rG'GwC_Cy
Cuts at: 639 639
Size: 4619
PstI C_TGCA'G
Cuts at: 1224 1307 1666 2351 1224
Size: 83 359 685 3492
Fragments arranged by size:
3492 685 359 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 803 1417 2044 2404 803
Size: 614 627 360 3018
Fragments arranged by size:
3018 627 614 360
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 1633 1633
Size: 4619
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SanDI GG'GwC_CC
Cuts at: 639 639
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 2642 2852 2642
Size: 210 4409
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 4136 4136
Size: 4619
SfcI C'TryA_G
Cuts at: 33 219 1220 1303 1662 2347 3340 3531 33
Size: 186 1001 83 359 685 993 191 1121
Fragments arranged by size:
1121 1001 993 685 359 191 186 83
SfoI GGC'GCC
Cuts at: 2051 2299 2051
Size: 248 4371
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1641 1641
Size: 4619
SmlI C'TyrA_G
Cuts at: 1774 2689 3181 3443 1774
Size: 915 492 262 2950
Fragments arranged by size:
2950 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
StyI C'CwwG_G
Cuts at: 107 595 943 1270 1753 2107 107
Size: 488 348 327 483 354 2619
Fragments arranged by size:
2619 488 483 354 348 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 2202 2335 2794 3003 196
Size: 2006 133 459 209 1812
Fragments arranged by size:
2006 1812 459 209 133
TatI w'GTAC_w
Cuts at: 497 738 4134 497
Size: 241 3396 982
Fragments arranged by size:
3396 982 241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 696 728 1493 1525 4136 4168 619
Size: 32 45 32 765 32 2611 32 1070
Fragments arranged by size:
2611 1070 765 45 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2416 2416
Size: 4619
XbaI T'CTAG_A
Cuts at: 1401 1650 1401
Size: 249 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2114
Size: 4619
XmaI C'CCGG_G
Cuts at: 1639 1639
Size: 4619
XmnI GAAnn'nnTTC
Cuts at: 1029 1029
Size: 4619
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BglI BmgBI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I CspCI
EcoNI FseI FspAI HindIII MfeI NdeI NotI NruI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI