Restriction map for
pF1KB8384
(Circular) (Six-base) MAPSORT of: pF1KB8384.seq Check: 6342 from: 1 to: 4961
pF1KB8384 4961 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1862 1999 1862
Size: 137 4824
Acc65I G'GTAC_C
Cuts at: 214 1977 214
Size: 1763 3198
AclI AA'CG_TT
Cuts at: 4801 4801
Size: 4961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 289 750 884 1160 1430 2786 3218 3965
Size: 105 461 134 276 270 1356 432 747
Cuts at: 3965 4233 184
Size: 268 912
Fragments arranged by size:
1356 912 747 461 432 276 270 268
134 105
AflII C'TTAA_G
Cuts at: 844 844
Size: 4961
AflIII A'CryG_T
Cuts at: 374 2313 3417 4394 374
Size: 1939 1104 977 941
Fragments arranged by size:
1939 1104 977 941
AgeI A'CCGG_T
Cuts at: 3349 3349
Size: 4961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 4961
AlwNI CAG_nnn'CTG
Cuts at: 1237 1570 2266 3833 1237
Size: 333 696 1567 2365
Fragments arranged by size:
2365 1567 696 333
ApaI G_GGCC'C
Cuts at: 159 676 1116 1744 159
Size: 517 440 628 3376
Fragments arranged by size:
3376 628 517 440
ApaLI G'TGCA_C
Cuts at: 3731 3731
Size: 4961
ApoI r'AATT_y
Cuts at: 271 1545 1778 1965 4314 4570 271
Size: 1274 233 187 2349 256 662
Fragments arranged by size:
2349 1274 662 256 233 187
AseI AT'TA_AT
Cuts at: 20 20
Size: 4961
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4961
AvaI C'yCGr_G
Cuts at: 695 884 1109 1334 1981 695
Size: 189 225 225 647 3675
Fragments arranged by size:
3675 647 225 225 189
BamHI G'GATC_C
Cuts at: 251 392 749 1986 251
Size: 141 357 1237 3226
Fragments arranged by size:
3226 1237 357 141
BanI G'GyrC_C
Cuts at: 214 1180 1224 1508 1977 2391 2639 2674 214
Size: 966 44 284 469 414 248 35 2501
Fragments arranged by size:
2501 966 469 414 284 248 44 35
BanII G_rGCy'C
Cuts at: 139 159 676 939 1045 1116 1311 1621 1744
Size: 20 517 263 106 71 195 310 123
Cuts at: 1744 1975 3005 139
Size: 231 1030 2095
Fragments arranged by size:
2095 1030 517 310 263 231 195 123
106 71 20
BbeI G_GCGC'C
Cuts at: 1512 2395 2643 1512
Size: 883 248 3830
Fragments arranged by size:
3830 883 248
BbsI GAAGACnn'nnnn_
Cuts at: 613 613
Size: 4961
BbvCI CC'TCA_GC
Cuts at: 173 173
Size: 4961
BciVI GTATCCnnnnn_n'
Cuts at: 1470 2859 3620 4961 1470
Size: 1389 761 1341 1470
Fragments arranged by size:
1470 1389 1341 761
BclI T'GATC_A
Cuts at: 2481 2481
Size: 4961
BglI GCCn_nnn'nGGC
Cuts at: 1084 1084
Size: 4961
BglII A'GATC_T
Cuts at: 530 2476 4513 530
Size: 1946 2037 978
Fragments arranged by size:
2037 1946 978
BlpI GC'TnA_GC
Cuts at: 2073 2073
Size: 4961
Bme1580I G_kGCm'C
Cuts at: 159 676 726 1116 1185 1227 1244 1342 1744
Size: 517 50 390 69 42 17 98 402
Cuts at: 1744 2586 2679 3735 159
Size: 842 93 1056 1385
Fragments arranged by size:
1385 1056 842 517 402 390 98 93
69 50 42 17
BmrI ACTGGGnnnn_n'
Cuts at: 155 747 1001 1135 1581 1948 2173 2254 2589
Size: 592 254 134 446 367 225 81 335
Cuts at: 2589 4733 155
Size: 2144 383
Fragments arranged by size:
2144 592 446 383 367 335 254 225
134 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3328 3328
Size: 4961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 1664 350
Size: 1314 3647
Bpu10I CC'TnA_GC
Cuts at: 173 1620 173
Size: 1447 3514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 1578 2137 3016 3508 3806 1543
Size: 35 559 879 492 298 2698
Fragments arranged by size:
2698 879 559 492 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 4665 4665
Size: 4961
BsaAI yAC'GTr
Cuts at: 1012 2314 1012
Size: 1302 3659
BsaBI GATnn'nnATC
Cuts at: 6 455 2475 4512 6
Size: 449 2020 2037 455
Fragments arranged by size:
2037 2020 455 449
BsaHI Gr'CG_yC
Cuts at: 1509 2392 2640 3342 4838 1509
Size: 883 248 702 1496 1632
Fragments arranged by size:
1632 1496 883 702 248
BsaWI w'CCGG_w
Cuts at: 2150 2671 3349 3623 3770 4181 2150
Size: 521 678 274 147 411 2930
Fragments arranged by size:
2930 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 703 733 361
Size: 30 312 30 4589
Fragments arranged by size:
4589 312 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 382
Size: 4961
BseYI C'CCAG_C
Cuts at: 178 687 962 1230 1751 2386 3144 3721 4217
Size: 509 275 268 521 635 758 577 496
Cuts at: 4217 178
Size: 922
Fragments arranged by size:
922 758 635 577 521 509 496 275
268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 812 1853 253
Size: 559 1041 3361
Fragments arranged by size:
3361 1041 559
BsiEI CG_ry'CG
Cuts at: 104 2503 2549 3757 104
Size: 2399 46 1208 1308
Fragments arranged by size:
2399 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 1045 1311 1975 2753 2943 3735 416
Size: 629 266 664 778 190 792 1642
Fragments arranged by size:
1642 792 778 664 629 266 190
Bsp1286I G_dGCh'C
Cuts at: 139 159 416 676 726 939 1045 1116 1185
Size: 20 257 260 50 213 106 71 69
Cuts at: 1185 1227 1244 1311 1342 1621 1744 1975 2586
Size: 42 17 67 31 279 123 231 611
Cuts at: 2586 2679 2753 2943 3005 3735 139
Size: 93 74 190 62 730 1365
Fragments arranged by size:
1365 730 611 279 260 257 231 213
190 123 106 93 74 71 69 67
62 50 42 31 20 17
BspEI T'CCGG_A
Cuts at: 4181 4181
Size: 4961
BspHI T'CATG_A
Cuts at: 4137 4137
Size: 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1059 1073 2011 2225 2527 2908 1059
Size: 14 938 214 302 381 3112
Fragments arranged by size:
3112 938 381 302 214 14
BsrBI CCG'CTC
Cuts at: 50 747 1020 3253 3307 4791 4956 50
Size: 697 273 2233 54 1484 165 55
Fragments arranged by size:
2233 1484 697 273 165 55 54
BsrDI GCAATG_nn'
Cuts at: 369 2873 369
Size: 2504 2457
BsrFI r'CCGG_y
Cuts at: 997 2959 3140 3349 997
Size: 1962 181 209 2609
Fragments arranged by size:
2609 1962 209 181
BsrGI T'GTAC_A
Cuts at: 264 264
Size: 4961
BssSI C'ACGA_G
Cuts at: 3232 3590 3232
Size: 358 4603
BstAPI GCAn_nnn'nTGC
Cuts at: 721 1489 1696 2338 721
Size: 768 207 642 3344
Fragments arranged by size:
3344 768 642 207
BstBI TT'CG_AA
Cuts at: 3322 3322
Size: 4961
BstEII G'GTnAC_C
Cuts at: 1369 1598 1369
Size: 229 4732
BstYI r'GATC_y
Cuts at: 1 251 392 530 749 975 1986 2453 2476
Size: 250 141 138 219 226 1011 467 23
Cuts at: 2476 2811 3057 4069 4155 4167 4513 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 1011 467 449 346 335 250 246
226 219 141 138 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 315 971 315
Size: 656 4305
BtgI C'CryG_G
Cuts at: 107 851 1574 1603 107
Size: 744 723 29 3465
Fragments arranged by size:
3465 744 723 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2911 3092 3255 2911
Size: 181 163 4617
Fragments arranged by size:
4617 181 163
BtsI GCAGTG_nn'
Cuts at: 1198 1865 2204 1198
Size: 667 339 3955
Fragments arranged by size:
3955 667 339
DraI TTT'AAA
Cuts at: 1960 1960
Size: 4961
DrdI GACnn_nn'nnGTC
Cuts at: 615 1393 2667 3525 615
Size: 778 1274 858 2051
Fragments arranged by size:
2051 1274 858 778
EaeI y'GGCC_r
Cuts at: 564 2546 2720 3111 3138 4483 564
Size: 1982 174 391 27 1345 1042
Fragments arranged by size:
1982 1345 1042 391 174 27
EagI C'GGCC_G
Cuts at: 2546 2546
Size: 4961
EarI CTCTTCn'nnn_
Cuts at: 404 860 1021 2984 3194 404
Size: 456 161 1963 210 2171
Fragments arranged by size:
2171 1963 456 210 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1121 3169 3479 3625 4771 1121
Size: 2048 310 146 1146 1311
Fragments arranged by size:
2048 1311 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1309 1973 1043
Size: 266 664 4031
Fragments arranged by size:
4031 664 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 289 350 750 884 1160 1430 1664 2786
Size: 105 61 400 134 276 270 234 1122
Cuts at: 2786 3218 3965 4233 184
Size: 432 747 268 912
Fragments arranged by size:
1122 912 747 432 400 276 270 268
234 134 105 61
EcoO109I rG'GnC_Cy
Cuts at: 155 156 319 1113 1740 1741 2100 155
Size: 1 163 794 627 1 359 3016
Fragments arranged by size:
3016 794 627 359 163 1 1
EcoRI G'AATT_C
Cuts at: 1545 1965 1545
Size: 420 4541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4961
FspI TGC'GCA
Cuts at: 2742 2742
Size: 4961
HaeII r_GCGC'y
Cuts at: 1512 2395 2643 3665 4644 1512
Size: 883 248 1022 979 1829
Fragments arranged by size:
1829 1022 979 883 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 2803 2833 2835 2865 325
Size: 32 2446 30 2 30 2421
Fragments arranged by size:
2446 2421 32 30 30 2
HincII GTy'rAC
Cuts at: 2000 2000
Size: 4961
KasI G'GCGC_C
Cuts at: 1508 2391 2639 1508
Size: 883 248 3830
Fragments arranged by size:
3830 883 248
KpnI G_GTAC'C
Cuts at: 218 1981 218
Size: 1763 3198
MluI A'CGCG_T
Cuts at: 4394 4394
Size: 4961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3632 3816 3632
Size: 184 4777
MscI TGG'CCA
Cuts at: 2722 2722
Size: 4961
MslI CAynn'nnrTG
Cuts at: 836 1252 2950 3077 836
Size: 416 1698 127 2720
Fragments arranged by size:
2720 1698 416 127
MspA1I CmG'CkG
Cuts at: 1237 1408 1570 1642 2072 2386 2746 3759 4004
Size: 171 162 72 430 314 360 1013 245
Cuts at: 4004 1237
Size: 2194
Fragments arranged by size:
2194 1013 430 360 314 245 171 162
72
NaeI GCC'GGC
Cuts at: 3142 3142
Size: 4961
NarI GG'CG_CC
Cuts at: 1509 2392 2640 1509
Size: 883 248 3830
Fragments arranged by size:
3830 883 248
NcoI C'CATG_G
Cuts at: 107 851 1574 1603 107
Size: 744 723 29 3465
Fragments arranged by size:
3465 744 723 29
NgoMIV G'CCGG_C
Cuts at: 3140 3140
Size: 4961
NspI r_CATG'y
Cuts at: 378 835 880 1903 2014 2514 3421 378
Size: 457 45 1023 111 500 907 1918
Fragments arranged by size:
1918 1023 907 500 457 111 45
PasI CC'CwG_GG
Cuts at: 1701 1701
Size: 4961
PciI A'CATG_T
Cuts at: 374 3417 374
Size: 3043 1918
PflMI CCAn_nnn'nTGG
Cuts at: 910 931 910
Size: 21 4940
PmeI GTTT'AAAC
Cuts at: 1960 1960
Size: 4961
PmlI CAC'GTG
Cuts at: 1012 1012
Size: 4961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4929
PpuMI rG'GwC_Cy
Cuts at: 319 319
Size: 4961
PshAI GACnn'nnGTC
Cuts at: 425 425
Size: 4961
PspOMI G'GGCC_C
Cuts at: 155 672 1112 1740 155
Size: 517 440 628 3376
Fragments arranged by size:
3376 628 517 440
PstI C_TGCA'G
Cuts at: 1070 2008 2693 1070
Size: 938 685 3338
Fragments arranged by size:
3338 938 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4961
PvuII CAG'CTG
Cuts at: 1237 1408 1570 1642 2386 2746 1237
Size: 171 162 72 744 360 3452
Fragments arranged by size:
3452 744 360 171 162 72
RsrII CG'GwC_CG
Cuts at: 3156 3156
Size: 4961
SacI G_AGCT'C
Cuts at: 1045 1311 1975 1045
Size: 266 664 4031
Fragments arranged by size:
4031 664 266
SalI G'TCGA_C
Cuts at: 1998 1998
Size: 4961
SapI GCTCTTCn'nnn_
Cuts at: 404 2984 3194 404
Size: 2580 210 2171
Fragments arranged by size:
2580 2171 210
SbfI CC_TGCA'GG
Cuts at: 1070 2008 1070
Size: 938 4023
ScaI AGT'ACT
Cuts at: 466 1694 4478 466
Size: 1228 2784 949
Fragments arranged by size:
2784 1228 949
SfcI C'TryA_G
Cuts at: 33 384 588 799 1066 2004 2689 3682 3873
Size: 351 204 211 267 938 685 993 191
Cuts at: 3873 33
Size: 1121
Fragments arranged by size:
1121 993 938 685 351 267 211 204
191
SfoI GGC'GCC
Cuts at: 1510 2393 2641 1510
Size: 883 248 3830
Fragments arranged by size:
3830 883 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4961
SmaI CCC'GGG
Cuts at: 1111 1336 1983 1111
Size: 225 647 4089
Fragments arranged by size:
4089 647 225
SmlI C'TyrA_G
Cuts at: 695 844 1558 1593 2116 3031 3523 3785 695
Size: 149 714 35 523 915 492 262 1871
Fragments arranged by size:
1871 915 714 523 492 262 149 35
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4961
SphI G_CATG'C
Cuts at: 2014 2514 2014
Size: 500 4461
SspI AAT'ATT
Cuts at: 583 583
Size: 4961
StyI C'CwwG_G
Cuts at: 107 224 611 851 1374 1574 1603 1784 2095
Size: 117 387 240 523 200 29 181 311
Cuts at: 2095 2449 107
Size: 354 2619
Fragments arranged by size:
2619 523 387 354 311 240 200 181
117 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2544 2677 3136 3345 2544
Size: 133 459 209 4160
Fragments arranged by size:
4160 459 209 133
TatI w'GTAC_w
Cuts at: 264 352 371 464 1692 4476 264
Size: 88 19 93 1228 2784 749
Fragments arranged by size:
2784 1228 749 93 88 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 453 485 703 735 1014 1046 4478 4510 453
Size: 32 218 32 279 32 3432 32 904
Fragments arranged by size:
3432 904 279 218 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2758 2758
Size: 4961
XbaI T'CTAG_A
Cuts at: 1992 1992
Size: 4961
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 2456 1610
Size: 846 4115
XhoI C'TCGA_G
Cuts at: 695 695
Size: 4961
XmaI C'CCGG_G
Cuts at: 1109 1334 1981 1109
Size: 225 647 4089
Fragments arranged by size:
4089 647 225
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PfoI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XmnI ZraI