Restriction map for
pF1KB8387
(Circular) (Six-base) MAPSORT of: pF1KB8387.seq Check: 4395 from: 1 to: 4817
pF1KB8387 4817 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 608 1855 608
Size: 1247 3570
Acc65I G'GTAC_C
Cuts at: 283 1085 1833 283
Size: 802 748 3267
Fragments arranged by size:
3267 802 748
AclI AA'CG_TT
Cuts at: 4657 4657
Size: 4817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1193 1700 1760 1802 2642 3074 3821 4089
Size: 513 507 60 42 840 432 747 268
Cuts at: 4089 680
Size: 1408
Fragments arranged by size:
1408 840 747 513 507 432 268 60
42
AflII C'TTAA_G
Cuts at: 415 454 415
Size: 39 4778
AflIII A'CryG_T
Cuts at: 1791 2169 3273 4250 1791
Size: 378 1104 977 2358
Fragments arranged by size:
2358 1104 977 378
AgeI A'CCGG_T
Cuts at: 889 3205 889
Size: 2316 2501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1350 1350
Size: 4817
AlwNI CAG_nnn'CTG
Cuts at: 2122 3689 2122
Size: 1567 3250
ApaLI G'TGCA_C
Cuts at: 3587 3587
Size: 4817
ApoI r'AATT_y
Cuts at: 225 868 1658 1821 4170 4426 225
Size: 643 790 163 2349 256 616
Fragments arranged by size:
2349 790 643 616 256 163
AseI AT'TA_AT
Cuts at: 20 20
Size: 4817
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
AvaI C'yCGr_G
Cuts at: 1220 1837 1220
Size: 617 4200
AvrII C'CTAG_G
Cuts at: 1380 1380
Size: 4817
BamHI G'GATC_C
Cuts at: 1842 1842
Size: 4817
BanI G'GyrC_C
Cuts at: 283 1085 1399 1833 2247 2495 2530 283
Size: 802 314 434 414 248 35 2570
Fragments arranged by size:
2570 802 434 414 314 248 35
BanII G_rGCy'C
Cuts at: 1831 2861 1831
Size: 1030 3787
BbeI G_GCGC'C
Cuts at: 2251 2499 2251
Size: 248 4569
BciVI GTATCCnnnnn_n'
Cuts at: 1290 2715 3476 4817 1290
Size: 1425 761 1341 1290
Fragments arranged by size:
1425 1341 1290 761
BclI T'GATC_A
Cuts at: 2337 2337
Size: 4817
BglI GCCn_nnn'nGGC
Cuts at: 133 133
Size: 4817
BglII A'GATC_T
Cuts at: 1551 2332 4369 1551
Size: 781 2037 1999
Fragments arranged by size:
2037 1999 781
BlpI GC'TnA_GC
Cuts at: 1929 1929
Size: 4817
Bme1580I G_kGCm'C
Cuts at: 1402 2442 2535 3591 1402
Size: 1040 93 1056 2628
Fragments arranged by size:
2628 1056 1040 93
BmrI ACTGGGnnnn_n'
Cuts at: 2029 2110 2445 4589 2029
Size: 81 335 2144 2257
Fragments arranged by size:
2257 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3184 3184
Size: 4817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 768 1525 524
Size: 244 757 3816
Fragments arranged by size:
3816 757 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 633 1774 1993 2872 3364 3662 633
Size: 1141 219 879 492 298 1788
Fragments arranged by size:
1788 1141 879 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 1422 1684 4521 1422
Size: 262 2837 1718
Fragments arranged by size:
2837 1718 262
BsaAI yAC'GTr
Cuts at: 2170 2170
Size: 4817
BsaBI GATnn'nnATC
Cuts at: 6 2331 4368 6
Size: 2325 2037 455
Fragments arranged by size:
2325 2037 455
BsaHI Gr'CG_yC
Cuts at: 2248 2496 3198 4694 2248
Size: 248 702 1496 2371
Fragments arranged by size:
2371 1496 702 248
BsaWI w'CCGG_w
Cuts at: 889 2006 2527 3205 3479 3626 4037 889
Size: 1117 521 678 274 147 411 1669
Fragments arranged by size:
1669 1117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1727 1757 1727
Size: 30 4787
BseYI C'CCAG_C
Cuts at: 202 2242 3000 3577 4073 202
Size: 2040 758 577 496 946
Fragments arranged by size:
2040 946 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 4817
BsiEI CG_ry'CG
Cuts at: 104 210 2359 2405 3613 104
Size: 106 2149 46 1208 1308
Fragments arranged by size:
2149 1308 1208 106 46
BsiHKAI G_wGCw'C
Cuts at: 1831 2609 2799 3591 1831
Size: 778 190 792 3057
Fragments arranged by size:
3057 792 778 190
BsiWI C'GTAC_G
Cuts at: 1004 1004
Size: 4817
BsmI GAATG_Cn'
Cuts at: 1221 1423 1221
Size: 202 4615
Bsp1286I G_dGCh'C
Cuts at: 1402 1831 2442 2535 2609 2799 2861 3591 1402
Size: 429 611 93 74 190 62 730 2628
Fragments arranged by size:
2628 730 611 429 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4037 4037
Size: 4817
BspHI T'CATG_A
Cuts at: 1061 1533 3993 1061
Size: 472 2460 1885
Fragments arranged by size:
2460 1885 472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1867 2081 2383 2764 1867
Size: 214 302 381 3920
Fragments arranged by size:
3920 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3109 3163 4647 4812 50
Size: 3059 54 1484 165 55
Fragments arranged by size:
3059 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2729 2729
Size: 4817
BsrFI r'CCGG_y
Cuts at: 164 889 2815 2996 3205 164
Size: 725 1926 181 209 1776
Fragments arranged by size:
1926 1776 725 209 181
BssSI C'ACGA_G
Cuts at: 3088 3446 3088
Size: 358 4459
BstAPI GCAn_nnn'nTGC
Cuts at: 2194 2194
Size: 4817
BstBI TT'CG_AA
Cuts at: 558 3178 558
Size: 2620 2197
BstXI CCAn_nnnn'nTGG
Cuts at: 1338 1554 1338
Size: 216 4601
BstYI r'GATC_y
Cuts at: 1 990 1551 1842 2309 2332 2667 2913 3925
Size: 989 561 291 467 23 335 246 1012
Cuts at: 3925 4011 4023 4369 1
Size: 86 12 346 449
Fragments arranged by size:
1012 989 561 467 449 346 335 291
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 609 609
Size: 4817
BtgI C'CryG_G
Cuts at: 131 1089 131
Size: 958 3859
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 192 2767 2948 3111 192
Size: 2575 181 163 1898
Fragments arranged by size:
2575 1898 181 163
BtsI GCAGTG_nn'
Cuts at: 2060 2060
Size: 4817
DraI TTT'AAA
Cuts at: 867 1816 867
Size: 949 3868
DrdI GACnn_nn'nnGTC
Cuts at: 575 2523 3381 575
Size: 1948 858 2011
Fragments arranged by size:
2011 1948 858
EaeI y'GGCC_r
Cuts at: 122 2402 2576 2967 2994 4339 122
Size: 2280 174 391 27 1345 600
Fragments arranged by size:
2280 1345 600 391 174 27
EagI C'GGCC_G
Cuts at: 2402 2402
Size: 4817
EarI CTCTTCn'nnn_
Cuts at: 2840 3050 2840
Size: 210 4607
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3025 3335 3481 4627 3025
Size: 310 146 1146 3215
Fragments arranged by size:
3215 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1829 1829
Size: 4817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 680 768 1193 1525 1700 1760 1802 2642
Size: 156 88 425 332 175 60 42 840
Cuts at: 2642 3074 3821 4089 524
Size: 432 747 268 1252
Fragments arranged by size:
1252 840 747 432 425 332 268 175
156 88 60 42
EcoO109I rG'GnC_Cy
Cuts at: 1956 1956
Size: 4817
EcoRI G'AATT_C
Cuts at: 1821 1821
Size: 4817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 430 469 2836 430
Size: 39 2367 2411
Fragments arranged by size:
2411 2367 39
FspI TGC'GCA
Cuts at: 2598 2598
Size: 4817
HaeII r_GCGC'y
Cuts at: 2251 2499 3521 4500 2251
Size: 248 1022 979 2568
Fragments arranged by size:
2568 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 580 612 2659 2689 2691 2721 457
Size: 32 91 32 2047 30 2 30 2553
Fragments arranged by size:
2553 2047 91 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1856 1856
Size: 4817
HindIII A'AGCT_T
Cuts at: 243 243
Size: 4817
KasI G'GCGC_C
Cuts at: 2247 2495 2247
Size: 248 4569
KpnI G_GTAC'C
Cuts at: 287 1089 1837 287
Size: 802 748 3267
Fragments arranged by size:
3267 802 748
MluI A'CGCG_T
Cuts at: 4250 4250
Size: 4817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3488 3672 3488
Size: 184 4633
MscI TGG'CCA
Cuts at: 2578 2578
Size: 4817
MslI CAynn'nnrTG
Cuts at: 1336 2806 2933 1336
Size: 1470 127 3220
Fragments arranged by size:
3220 1470 127
MspA1I CmG'CkG
Cuts at: 133 1928 2242 2602 3615 3860 133
Size: 1795 314 360 1013 245 1090
Fragments arranged by size:
1795 1090 1013 360 314 245
NaeI GCC'GGC
Cuts at: 166 2998 166
Size: 2832 1985
NarI GG'CG_CC
Cuts at: 2248 2496 2248
Size: 248 4569
NcoI C'CATG_G
Cuts at: 1089 1089
Size: 4817
NgoMIV G'CCGG_C
Cuts at: 164 2996 164
Size: 2832 1985
NspI r_CATG'y
Cuts at: 1795 1870 2370 3277 1795
Size: 75 500 907 3335
Fragments arranged by size:
3335 907 500 75
PciI A'CATG_T
Cuts at: 1791 3273 1791
Size: 1482 3335
PflMI CCAn_nnn'nTGG
Cuts at: 280 280
Size: 4817
PfoI T'CCnGG_A
Cuts at: 1502 1502
Size: 4817
PmeI GTTT'AAAC
Cuts at: 1816 1816
Size: 4817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4785
PsiI TTA'TAA
Cuts at: 1321 1321
Size: 4817
PstI C_TGCA'G
Cuts at: 1308 1864 2549 1308
Size: 556 685 3576
Fragments arranged by size:
3576 685 556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4817
PvuII CAG'CTG
Cuts at: 2242 2602 2242
Size: 360 4457
RsrII CG'GwC_CG
Cuts at: 3012 3012
Size: 4817
SacI G_AGCT'C
Cuts at: 1831 1831
Size: 4817
SacII CC_GC'GG
Cuts at: 134 134
Size: 4817
SalI G'TCGA_C
Cuts at: 1854 1854
Size: 4817
SapI GCTCTTCn'nnn_
Cuts at: 2840 3050 2840
Size: 210 4607
SbfI CC_TGCA'GG
Cuts at: 1864 1864
Size: 4817
ScaI AGT'ACT
Cuts at: 4334 4334
Size: 4817
SfcI C'TryA_G
Cuts at: 33 264 1304 1860 2545 3538 3729 33
Size: 231 1040 556 685 993 191 1121
Fragments arranged by size:
1121 1040 993 685 556 231 191
SfoI GGC'GCC
Cuts at: 2249 2497 2249
Size: 248 4569
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
SmaI CCC'GGG
Cuts at: 1839 1839
Size: 4817
SmlI C'TyrA_G
Cuts at: 415 454 612 1753 1972 2887 3379 3641 415
Size: 39 158 1141 219 915 492 262 1591
Fragments arranged by size:
1591 1141 915 492 262 219 158 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4817
SphI G_CATG'C
Cuts at: 1870 2370 1870
Size: 500 4317
SspI AAT'ATT
Cuts at: 740 1719 740
Size: 979 3838
StyI C'CwwG_G
Cuts at: 1089 1380 1951 2305 1089
Size: 291 571 354 3601
Fragments arranged by size:
3601 571 354 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1057 1381 2400 2533 2992 3201 1057
Size: 324 1019 133 459 209 2673
Fragments arranged by size:
2673 1019 459 324 209 133
TatI w'GTAC_w
Cuts at: 305 479 1273 1482 4332 305
Size: 174 794 209 2850 790
Fragments arranged by size:
2850 794 790 209 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 809 841 4334 4366 809
Size: 32 3493 32 1260
Fragments arranged by size:
3493 1260 32 32
Tth111I GACn'n_nGTC
Cuts at: 575 2614 575
Size: 2039 2778
XbaI T'CTAG_A
Cuts at: 546 1848 546
Size: 1302 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 2312
Size: 4817
XmaI C'CCGG_G
Cuts at: 1837 1837
Size: 4817
XmnI GAAnn'nnTTC
Cuts at: 246 493 554 1130 246
Size: 247 61 576 3933
Fragments arranged by size:
3933 576 247 61
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI