Restriction map for
pF1KB8390
(Circular) (Six-base) MAPSORT of: pF1KB8390.seq Check: 8917 from: 1 to: 4307
pF1KB8390 4307 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 264 416 637 1064 1154 264
Size: 152 221 427 90 3417
Fragments arranged by size:
3417 427 221 152 90
AatII G_ACGT'C
Cuts at: 1039 1039
Size: 4307
AccI GT'mk_AC
Cuts at: 1345 1345
Size: 4307
Acc65I G'GTAC_C
Cuts at: 1323 1323
Size: 4307
AclI AA'CG_TT
Cuts at: 4147 4147
Size: 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1066 2132 2564 3311 3579 1066
Size: 1066 432 747 268 1794
Fragments arranged by size:
1794 1066 747 432 268
AfeI AGC'GCT
Cuts at: 1171 1198 1171
Size: 27 4280
AflIII A'CryG_T
Cuts at: 995 1659 2763 3740 995
Size: 664 1104 977 1562
Fragments arranged by size:
1562 1104 977 664
AgeI A'CCGG_T
Cuts at: 2695 2695
Size: 4307
AlwNI CAG_nnn'CTG
Cuts at: 1612 3179 1612
Size: 1567 2740
ApaI G_GGCC'C
Cuts at: 756 838 756
Size: 82 4225
ApaLI G'TGCA_C
Cuts at: 3077 3077
Size: 4307
ApoI r'AATT_y
Cuts at: 1311 3660 3916 1311
Size: 2349 256 1702
Fragments arranged by size:
2349 1702 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
AvaI C'yCGr_G
Cuts at: 504 964 1183 1327 504
Size: 460 219 144 3484
Fragments arranged by size:
3484 460 219 144
BamHI G'GATC_C
Cuts at: 1332 1332
Size: 4307
BanI G'GyrC_C
Cuts at: 255 276 694 739 917 958 1142 1323 1737
Size: 21 418 45 178 41 184 181 414
Cuts at: 1737 1985 2020 255
Size: 248 35 2542
Fragments arranged by size:
2542 418 414 248 184 181 178 45
41 35 21
BanII G_rGCy'C
Cuts at: 301 756 838 1321 2351 301
Size: 455 82 483 1030 2257
Fragments arranged by size:
2257 1030 483 455 82
BbeI G_GCGC'C
Cuts at: 259 698 743 962 1741 1989 259
Size: 439 45 219 779 248 2577
Fragments arranged by size:
2577 779 439 248 219 45
BbsI GAAGACnn'nnnn_
Cuts at: 406 1039 406
Size: 633 3674
BbvCI CC'TCA_GC
Cuts at: 1265 1265
Size: 4307
BciVI GTATCCnnnnn_n'
Cuts at: 2205 2966 4307 2205
Size: 761 1341 2205
Fragments arranged by size:
2205 1341 761
BclI T'GATC_A
Cuts at: 1827 1827
Size: 4307
BglI GCCn_nnn'nGGC
Cuts at: 118 236 245 737 784 898 967 118
Size: 118 9 492 47 114 69 3458
Fragments arranged by size:
3458 492 118 114 69 47 9
BglII A'GATC_T
Cuts at: 1822 3859 1822
Size: 2037 2270
BlpI GC'TnA_GC
Cuts at: 1419 1419
Size: 4307
Bme1580I G_kGCm'C
Cuts at: 281 551 756 838 1932 2025 3081 281
Size: 270 205 82 1094 93 1056 1507
Fragments arranged by size:
1507 1094 1056 270 205 93 82
BmgBI CAC'GTC
Cuts at: 1230 1230
Size: 4307
BmrI ACTGGGnnnn_n'
Cuts at: 1519 1600 1935 4079 1519
Size: 81 335 2144 1747
Fragments arranged by size:
2144 1747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 291 139
Size: 152 4155
Bpu10I CC'TnA_GC
Cuts at: 1265 1265
Size: 4307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 2362 2854 3152 1483
Size: 879 492 298 2638
Fragments arranged by size:
2638 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4011 4011
Size: 4307
BsaAI yAC'GTr
Cuts at: 546 996 1005 1660 546
Size: 450 9 655 3193
Fragments arranged by size:
3193 655 450 9
BsaBI GATnn'nnATC
Cuts at: 6 1821 3858 6
Size: 1815 2037 455
Fragments arranged by size:
2037 1815 455
BsaHI Gr'CG_yC
Cuts at: 256 391 695 740 959 1036 1738 1986 2688
Size: 135 304 45 219 77 702 248 702
Cuts at: 2688 4184 256
Size: 1496 379
Fragments arranged by size:
1496 702 702 379 304 248 219 135
77 45
BsaWI w'CCGG_w
Cuts at: 128 1496 2017 2695 2969 3116 3527 128
Size: 1368 521 678 274 147 411 908
Fragments arranged by size:
1368 908 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 169 294 376 1732 2490 3067 3563 169
Size: 125 82 1356 758 577 496 913
Fragments arranged by size:
1356 913 758 577 496 125 82
BsiEI CG_ry'CG
Cuts at: 104 788 803 1849 1895 3103 104
Size: 684 15 1046 46 1208 1308
Fragments arranged by size:
1308 1208 1046 684 46 15
BsiHKAI G_wGCw'C
Cuts at: 1321 2099 2289 3081 1321
Size: 778 190 792 2547
Fragments arranged by size:
2547 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 281 301 551 756 838 1321 1932 2025 2099
Size: 20 250 205 82 483 611 93 74
Cuts at: 2099 2289 2351 3081 281
Size: 190 62 730 1507
Fragments arranged by size:
1507 730 611 483 250 205 190 93
82 74 62 20
BspEI T'CCGG_A
Cuts at: 128 3527 128
Size: 3399 908
BspHI T'CATG_A
Cuts at: 3483 3483
Size: 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 264 416 599 637 1064 1154 1357 1571 1873
Size: 152 183 38 427 90 203 214 302
Cuts at: 1873 2254 264
Size: 381 2317
Fragments arranged by size:
2317 427 381 302 214 203 183 152
90 38
BsrBI CCG'CTC
Cuts at: 50 2599 2653 4137 4302 50
Size: 2549 54 1484 165 55
Fragments arranged by size:
2549 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 208 2219 208
Size: 2011 2296
BsrFI r'CCGG_y
Cuts at: 339 486 2305 2486 2695 339
Size: 147 1819 181 209 1951
Fragments arranged by size:
1951 1819 209 181 147
BssHII G'CGCG_C
Cuts at: 533 533
Size: 4307
BssSI C'ACGA_G
Cuts at: 433 2578 2936 433
Size: 2145 358 1804
Fragments arranged by size:
2145 1804 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1684 1684
Size: 4307
BstBI TT'CG_AA
Cuts at: 2668 2668
Size: 4307
BstYI r'GATC_y
Cuts at: 1 1332 1799 1822 2157 2403 3415 3501 3513
Size: 1331 467 23 335 246 1012 86 12
Cuts at: 3513 3859 1
Size: 346 449
Fragments arranged by size:
1331 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 113 322 670 905 107
Size: 6 209 348 235 3509
Fragments arranged by size:
3509 348 235 209 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2257 2438 2601 2257
Size: 181 163 3963
Fragments arranged by size:
3963 181 163
BtsI GCAGTG_nn'
Cuts at: 358 1550 358
Size: 1192 3115
DraI TTT'AAA
Cuts at: 1306 1306
Size: 4307
DrdI GACnn_nn'nnGTC
Cuts at: 2013 2871 2013
Size: 858 3449
EaeI y'GGCC_r
Cuts at: 110 119 215 237 785 800 902 908 1892
Size: 9 96 22 548 15 102 6 984
Cuts at: 1892 2066 2457 2484 3829 110
Size: 174 391 27 1345 588
Fragments arranged by size:
1345 984 588 548 391 174 102 96
27 22 15 9 6
EagI C'GGCC_G
Cuts at: 785 800 1892 785
Size: 15 1092 3200
Fragments arranged by size:
3200 1092 15
EarI CTCTTCn'nnn_
Cuts at: 319 1105 2330 2540 319
Size: 786 1225 210 2086
Fragments arranged by size:
2086 1225 786 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 590 985 2515 2825 2971 4117 590
Size: 395 1530 310 146 1146 780
Fragments arranged by size:
1530 1146 780 395 310 146
EcoICRI GAG'CTC
Cuts at: 1319 1319
Size: 4307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 291 1066 2132 2564 3311 3579 139
Size: 152 775 1066 432 747 268 867
Fragments arranged by size:
1066 867 775 747 432 268 152
EcoO109I rG'GnC_Cy
Cuts at: 707 752 753 891 1296 1446 707
Size: 45 1 138 405 150 3568
Fragments arranged by size:
3568 405 150 138 45 1
EcoRI G'AATT_C
Cuts at: 1311 1311
Size: 4307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 4307
FspI TGC'GCA
Cuts at: 431 851 1207 2088 431
Size: 420 356 881 2650
Fragments arranged by size:
2650 881 420 356
HaeII r_GCGC'y
Cuts at: 176 259 698 743 962 1173 1200 1741 1989
Size: 83 439 45 219 211 27 541 248
Cuts at: 1989 3011 3990 176
Size: 1022 979 493
Fragments arranged by size:
1022 979 541 493 439 248 219 211
83 45 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2149 2179 2181 2211 2149
Size: 30 2 30 4245
Fragments arranged by size:
4245 30 30 2
HincII GTy'rAC
Cuts at: 1346 1346
Size: 4307
KasI G'GCGC_C
Cuts at: 255 694 739 958 1737 1985 255
Size: 439 45 219 779 248 2577
Fragments arranged by size:
2577 779 439 248 219 45
KpnI G_GTAC'C
Cuts at: 1327 1327
Size: 4307
MluI A'CGCG_T
Cuts at: 3740 3740
Size: 4307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 611 821 2978 3162 611
Size: 210 2157 184 1756
Fragments arranged by size:
2157 1756 210 184
MscI TGG'CCA
Cuts at: 2068 2068
Size: 4307
MslI CAynn'nnrTG
Cuts at: 2296 2423 2296
Size: 127 4180
MspA1I CmG'CkG
Cuts at: 115 530 790 926 932 1140 1418 1732 2092
Size: 415 260 136 6 208 278 314 360
Cuts at: 2092 3105 3350 115
Size: 1013 245 1072
Fragments arranged by size:
1072 1013 415 360 314 278 260 245
208 136 6
NaeI GCC'GGC
Cuts at: 341 2488 341
Size: 2147 2160
NarI GG'CG_CC
Cuts at: 256 695 740 959 1738 1986 256
Size: 439 45 219 779 248 2577
Fragments arranged by size:
2577 779 439 248 219 45
NcoI C'CATG_G
Cuts at: 107 107
Size: 4307
NgoMIV G'CCGG_C
Cuts at: 339 2486 339
Size: 2147 2160
NotI GC'GGCC_GC
Cuts at: 785 800 785
Size: 15 4292
NruI TCG'CGA
Cuts at: 1222 1222
Size: 4307
NspI r_CATG'y
Cuts at: 339 1360 1860 2767 339
Size: 1021 500 907 1879
Fragments arranged by size:
1879 1021 907 500
PciI A'CATG_T
Cuts at: 2763 2763
Size: 4307
PfoI T'CCnGG_A
Cuts at: 155 720 155
Size: 565 3742
PmeI GTTT'AAAC
Cuts at: 1306 1306
Size: 4307
PmlI CAC'GTG
Cuts at: 996 1005 996
Size: 9 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 4275
PpuMI rG'GwC_Cy
Cuts at: 1296 1296
Size: 4307
PspOMI G'GGCC_C
Cuts at: 752 834 752
Size: 82 4225
PspXI vC'TCGA_Gb
Cuts at: 504 504
Size: 4307
PstI C_TGCA'G
Cuts at: 146 275 413 884 1139 1354 2039 146
Size: 129 138 471 255 215 685 2414
Fragments arranged by size:
2414 685 471 255 215 138 129
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4307
PvuII CAG'CTG
Cuts at: 530 1732 2092 530
Size: 1202 360 2745
Fragments arranged by size:
2745 1202 360
RsrII CG'GwC_CG
Cuts at: 2502 2502
Size: 4307
SacI G_AGCT'C
Cuts at: 1321 1321
Size: 4307
SacII CC_GC'GG
Cuts at: 116 116
Size: 4307
SalI G'TCGA_C
Cuts at: 1344 1344
Size: 4307
SapI GCTCTTCn'nnn_
Cuts at: 319 1105 2330 2540 319
Size: 786 1225 210 2086
Fragments arranged by size:
2086 1225 786 210
SbfI CC_TGCA'GG
Cuts at: 275 1354 275
Size: 1079 3228
ScaI AGT'ACT
Cuts at: 3824 3824
Size: 4307
SexAI A'CCwGG_T
Cuts at: 1298 1298
Size: 4307
SfcI C'TryA_G
Cuts at: 33 142 225 271 409 880 1135 1350 2035
Size: 109 83 46 138 471 255 215 685
Cuts at: 2035 3028 3219 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 471 255 215 191 138
109 83 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 118 118
Size: 4307
SfoI GGC'GCC
Cuts at: 257 696 741 960 1739 1987 257
Size: 439 45 219 779 248 2577
Fragments arranged by size:
2577 779 439 248 219 45
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
SmaI CCC'GGG
Cuts at: 1329 1329
Size: 4307
SmlI C'TyrA_G
Cuts at: 504 1462 2377 2869 3131 504
Size: 958 915 492 262 1680
Fragments arranged by size:
1680 958 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4307
SphI G_CATG'C
Cuts at: 1360 1860 1360
Size: 500 3807
StuI AGG'CCT
Cuts at: 148 824 148
Size: 676 3631
StyI C'CwwG_G
Cuts at: 107 887 1441 1795 107
Size: 780 554 354 2619
Fragments arranged by size:
2619 780 554 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 660 1890 2023 2482 2691 660
Size: 1230 133 459 209 2276
Fragments arranged by size:
2276 1230 459 209 133
TatI w'GTAC_w
Cuts at: 3822 3822
Size: 4307
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4275
Tth111I GACn'n_nGTC
Cuts at: 2104 2104
Size: 4307
XbaI T'CTAG_A
Cuts at: 1338 1338
Size: 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 894 1802 114
Size: 780 908 2619
Fragments arranged by size:
2619 908 780
XhoI C'TCGA_G
Cuts at: 504 504
Size: 4307
XmaI C'CCGG_G
Cuts at: 1327 1327
Size: 4307
XmnI GAAnn'nnTTC
Cuts at: 1114 1114
Size: 4307
ZraI GAC'GTC
Cuts at: 1037 1037
Size: 4307
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NspI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NsiI PacI PasI PflMI PshAI PsiI
PsrI SanDI SgrAI SnaBI SrfI SspI SwaI