Restriction map for
pF1KB8397
(Circular) (Six-base) MAPSORT of: pF1KB8397.seq Check: 9914 from: 1 to: 4499
pF1KB8397 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1088 1537 1088
Size: 449 4050
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 1493 4339 1493
Size: 2846 1653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1202 2324 2756 3109 3771 664
Size: 538 1122 432 353 662 1392
Fragments arranged by size:
1392 1122 662 538 432 353
AflIII A'CryG_T
Cuts at: 850 1851 3651 3932 850
Size: 1001 1800 281 1417
Fragments arranged by size:
1800 1417 1001 281
AlwNI CAG_nnn'CTG
Cuts at: 1804 3242 1804
Size: 1438 3061
ApaLI G'TGCA_C
Cuts at: 3337 3337
Size: 4499
ApoI r'AATT_y
Cuts at: 1198 1420 1503 3852 4108 1198
Size: 222 83 2349 256 1589
Fragments arranged by size:
2349 1589 256 222 83
AseI AT'TA_AT
Cuts at: 20 1177 1183 20
Size: 1157 6 3336
Fragments arranged by size:
3336 1157 6
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 1519 1519
Size: 4499
AvrII C'CTAG_G
Cuts at: 1101 1101
Size: 4499
BamHI G'GATC_C
Cuts at: 1257 1524 1257
Size: 267 4232
BanI G'GyrC_C
Cuts at: 1047 1515 1929 2177 2212 1047
Size: 468 414 248 35 3334
Fragments arranged by size:
3334 468 414 248 35
BanII G_rGCy'C
Cuts at: 1247 1513 2543 1247
Size: 266 1030 3203
Fragments arranged by size:
3203 1030 266
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 202 1024 1196 202
Size: 822 172 3505
Fragments arranged by size:
3505 822 172
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3453 4499 2397
Size: 1056 1046 2397
Fragments arranged by size:
2397 1056 1046
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglII A'GATC_T
Cuts at: 2014 4051 2014
Size: 2037 2462
BlpI GC'TnA_GC
Cuts at: 1611 1611
Size: 4499
Bme1580I G_kGCm'C
Cuts at: 2124 2217 3341 2124
Size: 93 1124 3282
Fragments arranged by size:
3282 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 322 365 1019 1346 192
Size: 130 43 654 327 3345
Fragments arranged by size:
3345 654 327 130 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 907 1364 1675 2554 3268 3566 218
Size: 689 457 311 879 714 298 1151
Fragments arranged by size:
1151 879 714 689 457 311 298
BsaI GGTCTCn'nnnn_
Cuts at: 4203 4203
Size: 4499
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 1930 2178 2880 4376 1930
Size: 248 702 1496 2053
Fragments arranged by size:
2053 1496 702 248
BsaWI w'CCGG_w
Cuts at: 898 1688 2209 2887 3298 3445 3719 898
Size: 790 521 678 411 147 274 1678
Fragments arranged by size:
1678 790 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 463 463
Size: 4499
BseYI C'CCAG_C
Cuts at: 1485 1924 2682 3347 3755 1485
Size: 439 758 665 408 2229
Fragments arranged by size:
2229 758 665 439 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 884
Size: 4499
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3317 104
Size: 1937 46 1230 1286
Fragments arranged by size:
1937 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1513 2291 2481 3341 1513
Size: 778 190 860 2671
Fragments arranged by size:
2671 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1247 1513 2124 2217 2291 2481 2543 3341 1247
Size: 266 611 93 74 190 62 798 2405
Fragments arranged by size:
2405 798 611 266 190 93 74 62
BspEI T'CCGG_A
Cuts at: 898 898
Size: 4499
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 828 2791 2845 4329 4494 50
Size: 778 1963 54 1484 165 55
Fragments arranged by size:
1963 1484 778 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1231 2411 1231
Size: 1180 3319
BsrFI r'CCGG_y
Cuts at: 2497 2678 2497
Size: 181 4318
BssSI C'ACGA_G
Cuts at: 2770 3478 2770
Size: 708 3791
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstEII G'GTnAC_C
Cuts at: 1136 1136
Size: 4499
BstYI r'GATC_y
Cuts at: 1 1257 1524 1991 2014 2349 2595 2901 2913
Size: 1256 267 467 23 335 246 306 12
Cuts at: 2913 2999 4051 1
Size: 86 1052 449
Fragments arranged by size:
1256 1052 467 449 335 306 267 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 128 2449 2630 2793 128
Size: 2321 181 163 1834
Fragments arranged by size:
2321 1834 181 163
BtsI GCAGTG_nn'
Cuts at: 1742 1742
Size: 4499
DraI TTT'AAA
Cuts at: 1374 1498 1374
Size: 124 4375
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3549 2205
Size: 1344 3155
EaeI y'GGCC_r
Cuts at: 954 1077 2084 2258 2649 2676 4021 954
Size: 123 1007 174 391 27 1345 1432
Fragments arranged by size:
1432 1345 1007 391 174 123 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 142 440 494 1221 2522 2732 142
Size: 298 54 727 1301 210 1909
Fragments arranged by size:
1909 1301 727 298 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3449 3595 4309 2707
Size: 742 146 714 2897
Fragments arranged by size:
2897 742 714 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 322 365 664 1019 1202 1346 2324 2756
Size: 130 43 299 355 183 144 978 432
Cuts at: 2756 3109 3771 192
Size: 353 662 920
Fragments arranged by size:
978 920 662 432 355 353 299 183
144 130 43
EcoO109I rG'GnC_Cy
Cuts at: 1098 1638 1098
Size: 540 3959
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4499
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3411 4182 1933
Size: 248 1230 771 2250
Fragments arranged by size:
2250 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1213 1242 1245 1274 2341 2371 2373 2403 1213
Size: 29 3 29 1067 30 2 30 3309
Fragments arranged by size:
3309 1067 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 1538 1538
Size: 4499
HindIII A'AGCT_T
Cuts at: 1357 1357
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 493 908 3258 3442 493
Size: 415 2350 184 1550
Fragments arranged by size:
2350 1550 415 184
MscI TGG'CCA
Cuts at: 956 2260 956
Size: 1304 3195
MslI CAynn'nnrTG
Cuts at: 2488 2615 2488
Size: 127 4372
MspA1I CmG'CkG
Cuts at: 1610 1924 2284 3068 3313 1610
Size: 314 360 784 245 2796
Fragments arranged by size:
2796 784 360 314 245
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NspI r_CATG'y
Cuts at: 1485 1552 2052 3655 1485
Size: 67 500 1603 2329
Fragments arranged by size:
2329 1603 500 67
PciI A'CATG_T
Cuts at: 3651 3651
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 849 2945 2977 817
Size: 32 2096 32 2339
Fragments arranged by size:
2339 2096 32 32
PsiI TTA'TAA
Cuts at: 325 325
Size: 4499
PstI C_TGCA'G
Cuts at: 437 1546 2231 437
Size: 1109 685 2705
Fragments arranged by size:
2705 1109 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1924 2284 1924
Size: 360 4139
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 419 433 1542 2227 3195 3386 33
Size: 386 14 1109 685 968 191 1146
Fragments arranged by size:
1146 1109 968 685 386 191 14
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 233 922 1379 1654 2569 3283 3545 233
Size: 689 457 275 915 714 262 1187
Fragments arranged by size:
1187 915 714 689 457 275 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1485 1552 2052 1485
Size: 67 500 3932
Fragments arranged by size:
3932 500 67
SspI AAT'ATT
Cuts at: 745 1181 745
Size: 436 4063
StyI C'CwwG_G
Cuts at: 881 1101 1272 1633 1987 881
Size: 220 171 361 354 3393
Fragments arranged by size:
3393 361 354 220 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2082 2215 2674 2883 2082
Size: 133 459 209 3698
Fragments arranged by size:
3698 459 209 133
TatI w'GTAC_w
Cuts at: 4014 4014
Size: 4499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 705 737 893 925 4016 4048 705
Size: 32 156 32 3091 32 1156
Fragments arranged by size:
3091 1156 156 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
XmnI GAAnn'nnTTC
Cuts at: 1021 1021
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI