Restriction map for
pF1KB8398
(Circular) (Six-base) MAPSORT of: pF1KB8398.seq Check: 104 from: 1 to: 4997
pF1KB8398 4997 bp
With 182 enzymes: SgfI *
February 20, 2009 16:35 ..
AccI GT'mk_AC
Cuts at: 2035 2035
Size: 4997
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1565 2822 3254 3607 4269 1565
Size: 1257 432 353 662 2293
Fragments arranged by size:
2293 1257 662 432 353
AflIII A'CryG_T
Cuts at: 1535 2349 4149 4430 1535
Size: 814 1800 281 2102
Fragments arranged by size:
2102 1800 814 281
AhdI GACnn_n'nnGTC
Cuts at: 807 807
Size: 4997
AleI CACnn'nnGTG
Cuts at: 1036 1036
Size: 4997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 739 739
Size: 4997
AlwNI CAG_nnn'CTG
Cuts at: 1007 1360 1732 1968 2302 3740 1007
Size: 353 372 236 334 1438 2264
Fragments arranged by size:
2264 1438 372 353 334 236
ApaI G_GGCC'C
Cuts at: 1870 1870
Size: 4997
ApaLI G'TGCA_C
Cuts at: 595 3835 595
Size: 3240 1757
ApoI r'AATT_y
Cuts at: 2001 4350 4606 2001
Size: 2349 256 2392
Fragments arranged by size:
2392 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 327 1034 1495 1709 2017 327
Size: 707 461 214 308 3307
Fragments arranged by size:
3307 707 461 308 214
BamHI G'GATC_C
Cuts at: 1551 1899 2022 1551
Size: 348 123 4526
Fragments arranged by size:
4526 348 123
BanI G'GyrC_C
Cuts at: 233 257 292 332 844 1070 2013 2427 2675
Size: 24 35 40 512 226 943 414 248
Cuts at: 2675 2710 233
Size: 35 2520
Fragments arranged by size:
2520 943 512 414 248 226 40 35
35 24
BanII G_rGCy'C
Cuts at: 328 745 1394 1414 1502 1520 1652 1805 1856
Size: 417 649 20 88 18 132 153 51
Cuts at: 1856 1870 2011 3041 328
Size: 14 141 1030 2284
Fragments arranged by size:
2284 1030 649 417 153 141 132 88
51 20 18 14
BbeI G_GCGC'C
Cuts at: 336 2431 2679 336
Size: 2095 248 2654
Fragments arranged by size:
2654 2095 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 462 496 462
Size: 34 4963
BciVI GTATCCnnnnn_n'
Cuts at: 2895 3951 4997 2895
Size: 1056 1046 2895
Fragments arranged by size:
2895 1056 1046
BclI T'GATC_A
Cuts at: 188 2517 188
Size: 2329 2668
BglI GCCn_nnn'nGGC
Cuts at: 1102 1791 1102
Size: 689 4308
BglII A'GATC_T
Cuts at: 384 630 2512 4549 384
Size: 246 1882 2037 832
Fragments arranged by size:
2037 1882 832 246
BlpI GC'TnA_GC
Cuts at: 1393 2109 1393
Size: 716 4281
Bme1580I G_kGCm'C
Cuts at: 599 1073 1870 2622 2715 3839 599
Size: 474 797 752 93 1124 1757
Fragments arranged by size:
1757 1124 797 752 474 93
BmgBI CAC'GTC
Cuts at: 1536 1536
Size: 4997
BmrI ACTGGGnnnn_n'
Cuts at: 1029 1254 1496 2209 2290 2625 4769 1029
Size: 225 242 713 81 335 2144 1257
Fragments arranged by size:
2144 1257 713 335 242 225 81
BmtI G_CTAG'C
Cuts at: 166 166
Size: 4997
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1875 3364 1875
Size: 1489 3508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 661 1009 1159 1231 295
Size: 366 348 150 72 4061
Fragments arranged by size:
4061 366 348 150 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1213 1303 1324 1438 1867 2173 3052 3766
Size: 594 90 21 114 429 306 879 714
Cuts at: 3766 4064 619
Size: 298 1552
Fragments arranged by size:
1552 879 714 594 429 306 298 114
90 21
BsaI GGTCTCn'nnnn_
Cuts at: 271 4701 271
Size: 4430 567
BsaAI yAC'GTr
Cuts at: 2350 2350
Size: 4997
BsaBI GATnn'nnATC
Cuts at: 6 412 484 2511 4548 6
Size: 406 72 2027 2037 455
Fragments arranged by size:
2037 2027 455 406 72
BsaHI Gr'CG_yC
Cuts at: 333 2428 2676 3378 4874 333
Size: 2095 248 702 1496 456
Fragments arranged by size:
2095 1496 702 456 248
BsaWI w'CCGG_w
Cuts at: 356 947 1386 2186 2707 3385 3796 3943 4217
Size: 591 439 800 521 678 411 147 274
Cuts at: 4217 356
Size: 1136
Fragments arranged by size:
1136 800 678 591 521 439 411 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 814 1479 1582 1864 814
Size: 665 103 282 3947
Fragments arranged by size:
3947 665 282 103
BseYI C'CCAG_C
Cuts at: 2422 3180 3845 4253 2422
Size: 758 665 408 3166
Fragments arranged by size:
3166 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1179
Size: 4997
BsiEI CG_ry'CG
Cuts at: 104 155 172 2539 2585 3815 104
Size: 51 17 2367 46 1230 1286
Fragments arranged by size:
2367 1286 1230 51 46 17
BsiHKAI G_wGCw'C
Cuts at: 328 599 1394 1520 1652 2011 2789 2979 3839
Size: 271 795 126 132 359 778 190 860
Cuts at: 3839 328
Size: 1486
Fragments arranged by size:
1486 860 795 778 359 271 190 132
126
BsmI GAATG_Cn'
Cuts at: 1221 1334 1448 1221
Size: 113 114 4770
Fragments arranged by size:
4770 114 113
BsmBI CGTCTCn'nnnn_
Cuts at: 591 591
Size: 4997
Bsp1286I G_dGCh'C
Cuts at: 328 599 745 1073 1394 1414 1502 1520 1652
Size: 271 146 328 321 20 88 18 132
Cuts at: 1652 1805 1856 1870 2011 2622 2715 2789 2979
Size: 153 51 14 141 611 93 74 190
Cuts at: 2979 3041 3839 328
Size: 62 798 1486
Fragments arranged by size:
1486 798 611 328 321 271 190 153
146 141 132 93 88 74 62 51
20 18 14
BspEI T'CCGG_A
Cuts at: 356 1386 356
Size: 1030 3967
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 260 2047 2261 2563 2944 260
Size: 1787 214 302 381 2313
Fragments arranged by size:
2313 1787 381 302 214
BsrBI CCG'CTC
Cuts at: 50 537 3289 3343 4827 4992 50
Size: 487 2752 54 1484 165 55
Fragments arranged by size:
2752 1484 487 165 55 54
BsrDI GCAATG_nn'
Cuts at: 443 2909 443
Size: 2466 2531
BsrFI r'CCGG_y
Cuts at: 2995 3176 2995
Size: 181 4816
BssSI C'ACGA_G
Cuts at: 3268 3976 3268
Size: 708 4289
BstAPI GCAn_nnn'nTGC
Cuts at: 252 2374 252
Size: 2122 2875
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstXI CCAn_nnnn'nTGG
Cuts at: 1038 1303 1038
Size: 265 4732
BstYI r'GATC_y
Cuts at: 1 384 630 875 1551 1899 2022 2489 2512
Size: 383 246 245 676 348 123 467 23
Cuts at: 2512 2847 3093 3399 3411 3497 4549 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 676 467 449 383 348 335 306
246 246 245 123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1725 1882 1725
Size: 157 4840
BtgI C'CryG_G
Cuts at: 107 1100 107
Size: 993 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1282 1582 2947 3128 3291 1282
Size: 300 1365 181 163 2988
Fragments arranged by size:
2988 1365 300 181 163
BtsI GCAGTG_nn'
Cuts at: 1086 1289 1807 2240 1086
Size: 203 518 433 3843
Fragments arranged by size:
3843 518 433 203
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4997
DraIII CAC_nnn'GTG
Cuts at: 1186 1186
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 2703 4047 2703
Size: 1344 3653
EaeI y'GGCC_r
Cuts at: 152 169 341 998 2582 2756 3147 3174 4519
Size: 17 172 657 1584 174 391 27 1345
Cuts at: 4519 152
Size: 630
Fragments arranged by size:
1584 1345 657 630 391 174 172 27
17
EagI C'GGCC_G
Cuts at: 152 169 2582 152
Size: 17 2413 2567
Fragments arranged by size:
2567 2413 17
EarI CTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 3205 3947 4093 4807 193
Size: 3012 742 146 714 383
Fragments arranged by size:
3012 742 714 383 146
EcoICRI GAG'CTC
Cuts at: 326 1392 1518 1650 2009 326
Size: 1066 126 132 359 3314
Fragments arranged by size:
3314 1066 359 132 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 661 1009 1159 1231 1565 2822 3254 3607
Size: 366 348 150 72 334 1257 432 353
Cuts at: 3607 4269 295
Size: 662 1023
Fragments arranged by size:
1257 1023 662 432 366 353 348 334
150 72
EcoO109I rG'GnC_Cy
Cuts at: 902 1380 1866 1867 2136 902
Size: 478 486 1 269 3763
Fragments arranged by size:
3763 486 478 269 1
EcoRI G'AATT_C
Cuts at: 2001 2001
Size: 4997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4997
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 135 336 1218 1890 2431 2679 3909 4680 135
Size: 201 882 672 541 248 1230 771 452
Fragments arranged by size:
1230 882 771 672 541 452 248 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 793 825 1536 1568 2839 2869 2871
Size: 32 358 32 711 32 1271 30 2
Cuts at: 2871 2901 403
Size: 30 2499
Fragments arranged by size:
2499 1271 711 358 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1017 2036 1017
Size: 1019 3978
HindIII A'AGCT_T
Cuts at: 1577 1577
Size: 4997
KasI G'GCGC_C
Cuts at: 332 2427 2675 332
Size: 2095 248 2654
Fragments arranged by size:
2654 2095 248
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 3756 3940 722
Size: 3034 184 1779
Fragments arranged by size:
3034 1779 184
MscI TGG'CCA
Cuts at: 343 1000 2758 343
Size: 657 1758 2582
Fragments arranged by size:
2582 1758 657
MslI CAynn'nnrTG
Cuts at: 835 1036 1064 2986 3113 835
Size: 201 28 1922 127 2719
Fragments arranged by size:
2719 1922 201 127 28
MspA1I CmG'CkG
Cuts at: 174 806 1431 1776 1916 2108 2422 2782 3566
Size: 632 625 345 140 192 314 360 784
Cuts at: 3566 3811 174
Size: 245 1360
Fragments arranged by size:
1360 784 632 625 360 345 314 245
192 140
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 333 2428 2676 333
Size: 2095 248 2654
Fragments arranged by size:
2654 2095 248
NcoI C'CATG_G
Cuts at: 107 1100 107
Size: 993 4004
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NheI G'CTAG_C
Cuts at: 162 162
Size: 4997
NspI r_CATG'y
Cuts at: 1180 2050 2550 4153 1180
Size: 870 500 1603 2024
Fragments arranged by size:
2024 1603 870 500
PasI CC'CwG_GG
Cuts at: 282 813 1414 282
Size: 531 601 3865
Fragments arranged by size:
3865 601 531
PciI A'CATG_T
Cuts at: 4149 4149
Size: 4997
PflMI CCAn_nnn'nTGG
Cuts at: 1007 1007
Size: 4997
PfoI T'CCnGG_A
Cuts at: 272 1136 272
Size: 864 4133
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 3443
Size: 32 4965
PpuMI rG'GwC_Cy
Cuts at: 1380 1380
Size: 4997
PspOMI G'GGCC_C
Cuts at: 1866 1866
Size: 4997
PstI C_TGCA'G
Cuts at: 728 1365 2044 2729 728
Size: 637 679 685 2996
Fragments arranged by size:
2996 685 679 637
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 806 1776 1916 2422 2782 806
Size: 970 140 506 360 3021
Fragments arranged by size:
3021 970 506 360 140
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 4997
SacI G_AGCT'C
Cuts at: 328 1394 1520 1652 2011 328
Size: 1066 126 132 359 3314
Fragments arranged by size:
3314 1066 359 132 126
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 4514 4514
Size: 4997
SexAI A'CCwGG_T
Cuts at: 1182 1182
Size: 4997
SfcI C'TryA_G
Cuts at: 33 724 1361 2040 2725 3693 3884 33
Size: 691 637 679 685 968 191 1146
Fragments arranged by size:
1146 968 691 685 679 637 191
SfoI GGC'GCC
Cuts at: 334 2429 2677 334
Size: 2095 248 2654
Fragments arranged by size:
2654 2095 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 1711 2019 1711
Size: 308 4689
SmlI C'TyrA_G
Cuts at: 634 1228 1318 1339 1453 1846 2152 3067 3781
Size: 594 90 21 114 393 306 915 714
Cuts at: 3781 4043 634
Size: 262 1588
Fragments arranged by size:
1588 915 714 594 393 306 262 114
90 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4997
SphI G_CATG'C
Cuts at: 1180 2050 2550 1180
Size: 870 500 3627
Fragments arranged by size:
3627 870 500
SrfI GCCC'GGGC
Cuts at: 1711 1711
Size: 4997
StyI C'CwwG_G
Cuts at: 107 1100 1151 1556 1643 2131 2485 107
Size: 993 51 405 87 488 354 2619
Fragments arranged by size:
2619 993 488 405 354 87 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 2580 2713 3172 3381 805
Size: 1775 133 459 209 2421
Fragments arranged by size:
2421 1775 459 209 133
TatI w'GTAC_w
Cuts at: 4512 4512
Size: 4997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 4965
Tth111I GACn'n_nGTC
Cuts at: 2794 2794
Size: 4997
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1206 2492 114
Size: 1092 1286 2619
Fragments arranged by size:
2619 1286 1092
XmaI C'CCGG_G
Cuts at: 1709 2017 1709
Size: 308 4689
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI Bpu10I BsaXI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI